Rh2DG001600

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008755
Physical Location & Seq
Reverse (-)
145383 .. 147771
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG001600.1

Sequence Viewer

Length: 708 bp
ATGGCTTCTGCTGCTTCGGCTTCAAGCACTGGGTGCTTAGTTCGTTCACGCCTCCCACTCGGCGAATGTCAGCATCATCGTAATAACGGGATTGCACTGCTTCCCCAGTTACCTAAACCCCTCATCTCTCTTTCTTCTTCTTCTTCCAAAGCTGCTGCAATTGCAATTCCAATTCCAATTCCAACTGCAACTGCAAGTGAGAGGAAGGAGCGTTCCTCTTTTGCTCGAACTGCGGCTGTTCGGCATCTCGTGGGTTCCGTTACCACAGCTCACGGCCTTCGTTTTGCAGTGGTTGTAGCACGGTTTAATGAGATTGTCACAAAGCCACTGCTAGAGGGAGCCTTAACTACTTTCAACAATTATTCTGTTCAAGAAGAGGACATTGATGTGGTATGGGTTCCTGGTAGCTTTGAAATTGGTGTAGTTGCACAGAGGCTTGGAAGTTCGGGGAGATATCATGCAGTTTTATGCATTGGAGCTGTGATAAGAGGTGATACAACCCACTACGATGCTGTTGCTAATTCAGCTGCATCCGGAGTGCTTTCAGCTGGTTTAAAATCAGGTGTTCCATGCATATTCGGTGTCTTAACTTGTGATAATATGGAGCAGGCTTTAAATCGAGCTGGTGGTAAATCTGGAAATAAGGGTGCAGAGACTGCCTTGACAGCTATTGAGATGGCATCTTTGTTTGTGCACCAATTGAAGTAA

Protein Analysis

235

Amino Acids

24.46

Weight (kDa)

9.1

Isoelectric Point (pI)

35.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 92 - 229 1.1e-50 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 533
AciI CCGC 1 cut(s) 233
AdeI CACNNNGTG 1 cut(s) 33
AgsI TTSAA 5 cut(s) 24, 355, 371, 413, 703
AjnI CCWGG 1 cut(s) 400
AloI GAACNNNNNNTCC 2 cut(s) 196, 228
AluBI AGCT 8 cut(s) 152, 269, 408, 479, 527, 548, 623, 668
AluI AGCT 8 cut(s) 152, 269, 408, 479, 527, 548, 623, 668
Alw21I GWGCWC 1 cut(s) 696
Alw26I GTCTC 1 cut(s) 647
Alw44I GTGCAC 1 cut(s) 692
AlwNI CAGNNNCTG 1 cut(s) 656
Aor13HI TCCGGA 1 cut(s) 533
AoxI GGCC 1 cut(s) 274
ApaLI GTGCAC 1 cut(s) 692
ApeKI GCWGC 4 cut(s) 11, 152, 155, 527
AsuHPI GGTGA 1 cut(s) 503
BaeGI GKGCMC 1 cut(s) 696
BauI CACGAG 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 696
BbvI GCAGC 3 cut(s) 139, 142, 514
BccI CCATC 1 cut(s) 670
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 497, 531
BciT130I CCWGG 1 cut(s) 402
BcoDI GTCTC 1 cut(s) 647
BfaI CTAG 1 cut(s) 332
BisI GCNGC 5 cut(s) 12, 153, 156, 234, 528
BlsI GCNGC 5 cut(s) 13, 154, 157, 235, 529
Bme1390I CCNGG 1 cut(s) 402
BmiI GGNNCC 3 cut(s) 256, 340, 399
BmrFI CCNGG 1 cut(s) 402
BmrI ACTGGG 2 cut(s) 39, 100
BmsI GCATC 5 cut(s) 82, 253, 499, 539, 689
BmuI ACTGGG 2 cut(s) 39, 100
BplI GAGNNNNNCTC 2 cut(s) 200, 232
BsaWI WCCGGW 1 cut(s) 533
Bse1I ACTGG 2 cut(s) 34, 106
BseAI TCCGGA 1 cut(s) 533
BseBI CCWGG 1 cut(s) 402
BseGI GGATG 1 cut(s) 530
BseNI ACTGG 2 cut(s) 34, 106
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 3 cut(s) 139, 142, 514
BsgI GTGCAG 1 cut(s) 669
BshFI GGCC 1 cut(s) 276
BsiHKAI GWGCWC 1 cut(s) 696
BsiSI CCGG 1 cut(s) 534
BsmAI GTCTC 1 cut(s) 647
BsnI GGCC 1 cut(s) 276
Bsp1286I GDGCHC 1 cut(s) 696
Bsp13I TCCGGA 1 cut(s) 533
BspACI CCGC 1 cut(s) 233
BspANI GGCC 1 cut(s) 276
BspEI TCCGGA 1 cut(s) 533
BspLI GGNNCC 3 cut(s) 256, 340, 399
BsrI ACTGG 2 cut(s) 34, 106
BssSI CACGAG 1 cut(s) 248
Bst2BI CACGAG 1 cut(s) 248
Bst2UI CCWGG 1 cut(s) 402
Bst4CI ACNGT 1 cut(s) 303
Bst6I CTCTTC 1 cut(s) 369
BstAPI GCANNNNNTGC 2 cut(s) 33, 656
BstC8I GCNNGC 1 cut(s) 609
BstDEI CTNAG 1 cut(s) 37
BstF5I GGATG 1 cut(s) 530
BstMAI GTCTC 1 cut(s) 647
BstMWI GCNNNNNNNGC 8 cut(s) 11, 17, 33, 161, 230, 524, 656, 665
BstNI CCWGG 1 cut(s) 402
BstSCI CCNGG 1 cut(s) 400
BstSLI GKGCMC 1 cut(s) 696
BstV1I GCAGC 3 cut(s) 139, 142, 514
BsuRI GGCC 1 cut(s) 276
BtsCI GGATG 1 cut(s) 530
BtsI GCAGTG 3 cut(s) 95, 294, 326
BtsIMutI CAGTG 4 cut(s) 27, 95, 294, 326
Cac8I GCNNGC 1 cut(s) 609
CaiI CAGNNNCTG 1 cut(s) 656
CviAII CATG 2 cut(s) 458, 570
DdeI CTNAG 1 cut(s) 37
DraI TTTAAA 2 cut(s) 555, 615
DraIII CACNNNGTG 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 369
EarI CTCTTC 1 cut(s) 369
Eco32I GATATC 1 cut(s) 455
EcoRII CCWGG 1 cut(s) 400
EcoRV GATATC 1 cut(s) 455
EcoT22I ATGCAT 2 cut(s) 473, 575
FaeI CATG 2 cut(s) 461, 573
FaiI YATR 6 cut(s) 394, 459, 469, 571, 575, 602
FatI CATG 2 cut(s) 457, 569
Fnu4HI GCNGC 5 cut(s) 12, 153, 156, 234, 528
FokI GGATG 1 cut(s) 517
Fsp4HI GCNGC 5 cut(s) 12, 153, 156, 234, 528
FspBI CTAG 1 cut(s) 332
GluI GCNGC 5 cut(s) 12, 153, 156, 234, 528
HaeIII GGCC 1 cut(s) 276
HapII CCGG 1 cut(s) 534
Hin1II CATG 2 cut(s) 461, 573
HpaII CCGG 1 cut(s) 534
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 2 cut(s) 47, 694
Hpy188III TCNNGA 3 cut(s) 371, 534, 636
Hpy8I GTNNAC 2 cut(s) 47, 694
HpyAV CCTTC 2 cut(s) 199, 287
HpyCH4III ACNGT 1 cut(s) 303
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 17, 33, 161, 230, 524, 656, 665
HpyF3I CTNAG 1 cut(s) 37
Hsp92II CATG 2 cut(s) 461, 573
Kpn2I TCCGGA 1 cut(s) 533
LmnI GCTCC 4 cut(s) 208, 338, 476, 604
Lsp1109I GCAGC 3 cut(s) 139, 142, 514
LweI GCATC 5 cut(s) 82, 253, 499, 539, 689
MaeI CTAG 1 cut(s) 332
MaeIII GTNAC 3 cut(s) 108, 259, 316
MboII GAAGA 5 cut(s) 126, 129, 132, 135, 386
MfeI CAATTG 2 cut(s) 159, 698
MhlI GDGCHC 1 cut(s) 696
MluCI AATT 8 cut(s) 159, 165, 171, 177, 358, 414, 520, 698
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 8 cut(s) 62, 131, 195, 226, 328, 370, 426, 482
Mph1103I ATGCAT 2 cut(s) 473, 575
MroI TCCGGA 1 cut(s) 533
MseI TTAA 5 cut(s) 306, 344, 554, 587, 614
MslI CAYNNNNRTG 2 cut(s) 386, 507
MspA1I CMGCKG 2 cut(s) 527, 548
MspI CCGG 1 cut(s) 534
MspR9I CCNGG 1 cut(s) 402
MunI CAATTG 2 cut(s) 159, 698
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 8 cut(s) 11, 17, 33, 161, 230, 524, 656, 665
NlaIII CATG 2 cut(s) 461, 573
NlaIV GGNNCC 3 cut(s) 256, 340, 399
NmeAIII GCCGAG 1 cut(s) 39
NmuCI GTSAC 1 cut(s) 316
NsiI ATGCAT 2 cut(s) 473, 575
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PkrI GCNGC 5 cut(s) 13, 154, 157, 235, 529
Psp6I CCWGG 1 cut(s) 400
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 3 cut(s) 256, 340, 399
PstNI CAGNNNCTG 1 cut(s) 656
PvuII CAGCTG 2 cut(s) 527, 548
RseI CAYNNNNRTG 2 cut(s) 386, 507
SaqAI TTAA 5 cut(s) 306, 344, 554, 587, 614
SatI GCNGC 5 cut(s) 12, 153, 156, 234, 528
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 1 cut(s) 696
SfaNI GCATC 5 cut(s) 82, 253, 499, 539, 689
SmiMI CAYNNNNRTG 2 cut(s) 386, 507
Sse9I AATT 8 cut(s) 159, 165, 171, 177, 358, 414, 520, 698
SsiI CCGC 1 cut(s) 233
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 1 cut(s) 400
TaaI ACNGT 1 cut(s) 303
TaqI TCGA 2 cut(s) 226, 619
TasI AATT 8 cut(s) 159, 165, 171, 177, 358, 414, 520, 698
TauI GCSGC 1 cut(s) 236
Tru1I TTAA 5 cut(s) 306, 344, 554, 587, 614
Tru9I TTAA 5 cut(s) 306, 344, 554, 587, 614
TscAI CASTG 4 cut(s) 34, 102, 294, 333
TseFI GTSAC 1 cut(s) 316
TseI GCWGC 4 cut(s) 11, 152, 155, 527
Tsp45I GTSAC 1 cut(s) 316
TspGWI ACGGA 1 cut(s) 247
TspRI CASTG 4 cut(s) 34, 102, 294, 333
VneI GTGCAC 1 cut(s) 692
XspI CTAG 1 cut(s) 332
Zsp2I ATGCAT 2 cut(s) 473, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.