RchiOBHm_Chr7g0227511

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008755
Physical Location & Seq
Reverse (-)
50742100 .. 50745429
3330 bp
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UTR
Exon/CDS
Intron
PRQ20378

Sequence Viewer

Length: 447 bp
ATGATTTTGTTTTCTTCTGTCTTCAAGGTTGTAGCACGGTTTAATGAGATTGTCACAAAGCCAATGCTAGAGGGAGCCTTAACTACTTTCAACAATTATTCTGTTCAAGAAGAGGACATTGATGTGGTATGGGTTCCTGGTAGCTTTGAGATTGGTGTAGTTGCACAGAGGCTTGGAAGTTCGGGGAGATATCATGCAGTTTTATGCATTGGAGCTGTGATAAGAGGTGATACAACGCACTACGATGCTGTTGCTAATTCAGCTGCATCCAGAGTGCTTTCAGCTGGTTTAAAATCAGGTGTTCCATGCATATTCGGTGTCTTAACTTGTGATAATATGGAGCAGGCTTTAAATCGAGCTGGTGGTAAATCTGGAAATAAGGGTGCAAAGACTGCCTTGACAGCTGTAAGTTTGGACCAACTAGTTTCTGTGCTTTGTTCTGACTAG

Protein Analysis

148

Amino Acids

15.59

Weight (kDa)

6.08

Isoelectric Point (pI)

25.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 10 - 138 2.2e-45 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 3 cut(s) 25, 91, 107
AhlI ACTAGT 1 cut(s) 421
AjnI CCWGG 1 cut(s) 136
AluBI AGCT 6 cut(s) 144, 215, 263, 284, 359, 404
AluI AGCT 6 cut(s) 144, 215, 263, 284, 359, 404
ApeKI GCWGC 1 cut(s) 263
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 1 cut(s) 239
AvaII GGWCC 1 cut(s) 415
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 250
BcgI CGANNNNNNTGC 2 cut(s) 233, 267
BciT130I CCWGG 1 cut(s) 138
BcuI ACTAGT 1 cut(s) 421
BfaI CTAG 3 cut(s) 68, 422, 445
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 1 cut(s) 138
Bme18I GGWCC 1 cut(s) 415
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 2 cut(s) 76, 135
BmrFI CCNGG 1 cut(s) 138
BmsI GCATC 2 cut(s) 235, 275
BpiI GAAGAC 1 cut(s) 13
BseBI CCWGG 1 cut(s) 138
BseGI GGATG 1 cut(s) 266
BseXI GCAGC 1 cut(s) 250
BspLI GGNNCC 2 cut(s) 76, 135
Bst2UI CCWGG 1 cut(s) 138
Bst4CI ACNGT 1 cut(s) 39
Bst6I CTCTTC 1 cut(s) 105
BstAPI GCANNNNNTGC 1 cut(s) 392
BstC8I GCNNGC 1 cut(s) 345
BstF5I GGATG 1 cut(s) 266
BstMWI GCNNNNNNNGC 3 cut(s) 260, 392, 401
BstNI CCWGG 1 cut(s) 138
BstSCI CCNGG 1 cut(s) 136
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 13
BtsCI GGATG 1 cut(s) 266
Cac8I GCNNGC 1 cut(s) 345
Cfr13I GGNCC 1 cut(s) 415
CviAII CATG 2 cut(s) 194, 306
DraI TTTAAA 2 cut(s) 291, 351
Eam1104I CTCTTC 1 cut(s) 105
EarI CTCTTC 1 cut(s) 105
Eco32I GATATC 1 cut(s) 191
Eco47I GGWCC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 136
EcoRV GATATC 1 cut(s) 191
EcoT22I ATGCAT 2 cut(s) 209, 311
FaeI CATG 2 cut(s) 197, 309
FaiI YATR 6 cut(s) 130, 195, 205, 307, 311, 338
FalI AAGNNNNNCTT 2 cut(s) 380, 412
FatI CATG 2 cut(s) 193, 305
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 253
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 3 cut(s) 68, 422, 445
GluI GCNGC 1 cut(s) 264
Hin1II CATG 2 cut(s) 197, 309
HphI GGTGA 1 cut(s) 239
Hpy188I TCNGA 1 cut(s) 442
Hpy188III TCNNGA 3 cut(s) 107, 270, 372
HpyCH4III ACNGT 1 cut(s) 39
HpyCH4V TGCA 6 cut(s) 164, 197, 207, 266, 309, 386
HpyF10VI GCNNNNNNNGC 3 cut(s) 260, 392, 401
Hsp92II CATG 2 cut(s) 197, 309
LmnI GCTCC 3 cut(s) 74, 212, 340
LpnPI CCDG 8 cut(s) 123, 150, 270, 282, 283, 329, 345, 357
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 2 cut(s) 235, 275
MaeI CTAG 3 cut(s) 68, 422, 445
MaeIII GTNAC 1 cut(s) 52
MboII GAAGA 3 cut(s) 6, 13, 122
MluCI AATT 2 cut(s) 94, 256
MnlI CCTC 4 cut(s) 64, 106, 162, 218
Mph1103I ATGCAT 2 cut(s) 209, 311
MseI TTAA 5 cut(s) 42, 80, 290, 323, 350
MslI CAYNNNNRTG 2 cut(s) 122, 243
MspA1I CMGCKG 3 cut(s) 263, 284, 404
MspR9I CCNGG 1 cut(s) 138
MvaI CCWGG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 260, 392, 401
NlaIII CATG 2 cut(s) 197, 309
NlaIV GGNNCC 2 cut(s) 76, 135
NmuCI GTSAC 1 cut(s) 52
NsiI ATGCAT 2 cut(s) 209, 311
PkrI GCNGC 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 136
PspN4I GGNNCC 2 cut(s) 76, 135
PspPI GGNCC 1 cut(s) 415
PvuII CAGCTG 3 cut(s) 263, 284, 404
RseI CAYNNNNRTG 2 cut(s) 122, 243
SaqAI TTAA 5 cut(s) 42, 80, 290, 323, 350
SatI GCNGC 1 cut(s) 264
Sau96I GGNCC 1 cut(s) 415
ScrFI CCNGG 1 cut(s) 138
SetI ASST 9 cut(s) 30, 146, 217, 229, 265, 286, 301, 361, 406
SfaNI GCATC 2 cut(s) 235, 275
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 2 cut(s) 122, 243
SpeI ACTAGT 1 cut(s) 421
Sse9I AATT 2 cut(s) 94, 256
SspMI CTAG 3 cut(s) 68, 422, 445
StyD4I CCNGG 1 cut(s) 136
TaaI ACNGT 1 cut(s) 39
TaqI TCGA 1 cut(s) 355
TasI AATT 2 cut(s) 94, 256
Tru1I TTAA 5 cut(s) 42, 80, 290, 323, 350
Tru9I TTAA 5 cut(s) 42, 80, 290, 323, 350
TseFI GTSAC 1 cut(s) 52
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 52
VpaK11BI GGWCC 1 cut(s) 415
XspI CTAG 3 cut(s) 68, 422, 445
Zsp2I ATGCAT 2 cut(s) 209, 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.