RchiOBHm_Chr7g0214271

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0008755
Physical Location & Seq
Forward (+)
31518126 .. 31522535
4410 bp
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UTR
Exon/CDS
Intron
PRQ19174

Sequence Viewer

Length: 324 bp
ATGTATGTGGTATGGGTTCCTGGTAGCTTTGAGATTGGTGTAGTTGCACAGAGGCTTGGAAGTTCGGGGAGATATCATGCAGTTTTATGCATTGGAGCTGTGATAAGAGGTGATACAACCCACTACGATGCTGTTGCTAATTCAGCTGCATCCGGAGTGCTTTCAGCTGGTTTAAAATCAGATGTTCCATGCATATTCGGTGTCTTAACTTGTGATAATATGGAGCAGGCTTTAAATCGAGCTGGTGGTAAATCTGGAAATAAGGGTGTAGAGACTGCCTTGACAGCTGTAAAATTATATGATGAAGTACTTCAAAGAGGATGA

Protein Analysis

107

Amino Acids

11.13

Weight (kDa)

6.7

Isoelectric Point (pI)

28.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 2 - 100 4e-36 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 152
AfaI GTAC 1 cut(s) 309
AgsI TTSAA 1 cut(s) 314
AjnI CCWGG 1 cut(s) 19
AluBI AGCT 6 cut(s) 27, 98, 146, 167, 242, 287
AluI AGCT 6 cut(s) 27, 98, 146, 167, 242, 287
Alw26I GTCTC 1 cut(s) 266
Aor13HI TCCGGA 1 cut(s) 152
ApeKI GCWGC 1 cut(s) 146
Asp700I GAANNNNTTC 1 cut(s) 309
AsuHPI GGTGA 1 cut(s) 122
BbvI GCAGC 1 cut(s) 133
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BciT130I CCWGG 1 cut(s) 21
BcoDI GTCTC 1 cut(s) 266
BisI GCNGC 1 cut(s) 147
BlsI GCNGC 1 cut(s) 148
BmcAI AGTACT 1 cut(s) 309
Bme1390I CCNGG 1 cut(s) 21
BmiI GGNNCC 1 cut(s) 18
BmrFI CCNGG 1 cut(s) 21
BmsI GCATC 2 cut(s) 118, 158
BsaWI WCCGGW 1 cut(s) 152
BseAI TCCGGA 1 cut(s) 152
BseBI CCWGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 149
BseXI GCAGC 1 cut(s) 133
BsiSI CCGG 1 cut(s) 153
BsmAI GTCTC 1 cut(s) 266
Bsp13I TCCGGA 1 cut(s) 152
BspEI TCCGGA 1 cut(s) 152
BspLI GGNNCC 1 cut(s) 18
Bst2UI CCWGG 1 cut(s) 21
BstC8I GCNNGC 1 cut(s) 228
BstF5I GGATG 1 cut(s) 149
BstMAI GTCTC 1 cut(s) 266
BstMWI GCNNNNNNNGC 2 cut(s) 143, 284
BstNI CCWGG 1 cut(s) 21
BstSCI CCNGG 1 cut(s) 19
BstV1I GCAGC 1 cut(s) 133
BtsCI GGATG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 308
CviAII CATG 2 cut(s) 77, 189
CviJI RGCY 8 cut(s) 27, 55, 98, 146, 167, 230, 242, 287
CviKI_1 RGCY 8 cut(s) 27, 55, 98, 146, 167, 230, 242, 287
CviQI GTAC 1 cut(s) 308
DraI TTTAAA 2 cut(s) 174, 234
Eco32I GATATC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 19
EcoRV GATATC 1 cut(s) 74
EcoT22I ATGCAT 2 cut(s) 92, 194
FaeI CATG 2 cut(s) 80, 192
FaiI YATR 9 cut(s) 6, 13, 78, 88, 190, 194, 221, 298, 300
FatI CATG 2 cut(s) 76, 188
Fnu4HI GCNGC 1 cut(s) 147
FokI GGATG 1 cut(s) 136
Fsp4HI GCNGC 1 cut(s) 147
GluI GCNGC 1 cut(s) 147
HapII CCGG 1 cut(s) 153
Hin1II CATG 2 cut(s) 80, 192
HpaII CCGG 1 cut(s) 153
HphI GGTGA 1 cut(s) 122
Hpy188I TCNGA 1 cut(s) 181
Hpy188III TCNNGA 2 cut(s) 153, 255
HpyCH4V TGCA 5 cut(s) 47, 80, 90, 149, 192
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 284
Hsp92II CATG 2 cut(s) 80, 192
Kpn2I TCCGGA 1 cut(s) 152
LmnI GCTCC 2 cut(s) 95, 223
LpnPI CCDG 7 cut(s) 6, 33, 153, 166, 212, 228, 240
Lsp1109I GCAGC 1 cut(s) 133
LweI GCATC 2 cut(s) 118, 158
MluCI AATT 2 cut(s) 139, 293
MnlI CCTC 3 cut(s) 45, 101, 311
Mph1103I ATGCAT 2 cut(s) 92, 194
MroI TCCGGA 1 cut(s) 152
MroXI GAANNNNTTC 1 cut(s) 309
MseI TTAA 3 cut(s) 173, 206, 233
MslI CAYNNNNRTG 1 cut(s) 126
MspA1I CMGCKG 3 cut(s) 146, 167, 287
MspI CCGG 1 cut(s) 153
MspR9I CCNGG 1 cut(s) 21
MvaI CCWGG 1 cut(s) 21
MwoI GCNNNNNNNGC 2 cut(s) 143, 284
NlaIII CATG 2 cut(s) 80, 192
NlaIV GGNNCC 1 cut(s) 18
NsiI ATGCAT 2 cut(s) 92, 194
PdmI GAANNNNTTC 1 cut(s) 309
PkrI GCNGC 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 19
PspGI CCWGG 1 cut(s) 19
PspN4I GGNNCC 1 cut(s) 18
PvuII CAGCTG 3 cut(s) 146, 167, 287
RsaI GTAC 1 cut(s) 309
RsaNI GTAC 1 cut(s) 308
RseI CAYNNNNRTG 1 cut(s) 126
SaqAI TTAA 3 cut(s) 173, 206, 233
SatI GCNGC 1 cut(s) 147
ScaI AGTACT 1 cut(s) 309
ScrFI CCNGG 1 cut(s) 21
SetI ASST 7 cut(s) 29, 100, 112, 148, 169, 244, 289
SfaNI GCATC 2 cut(s) 118, 158
SmiMI CAYNNNNRTG 1 cut(s) 126
Sse9I AATT 2 cut(s) 139, 293
StyD4I CCNGG 1 cut(s) 19
TaqI TCGA 1 cut(s) 238
TasI AATT 2 cut(s) 139, 293
TatI WGTACW 1 cut(s) 307
Tru1I TTAA 3 cut(s) 173, 206, 233
Tru9I TTAA 3 cut(s) 173, 206, 233
TseI GCWGC 1 cut(s) 146
TspDTI ATGAA 1 cut(s) 318
XmnI GAANNNNTTC 1 cut(s) 309
ZrmI AGTACT 1 cut(s) 309
Zsp2I ATGCAT 2 cut(s) 92, 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.