Rw4G009510

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0008755
Physical Location & Seq
Forward (+)
21444358 .. 21448096
3739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G009510.1

Sequence Viewer

Length: 648 bp
ATGGAGGACAACGCGTCGAGCCCAACACGGCGCTATTTAATCTCGTCCTCAAGGCTATGCACGGCGGTAAAGAGTCCGAAGCCGCTAATGTTGAGTACAGTGAGTCATGAGAGGAAGGAGCGTTCCTCTTTTGCTCGAACTGCGGCTGTTCGGCATCTGGTAGGTTCCTTTACCACAGCTCACGGCCTTCGTTTCGCAGTGGTTGCAGCACGGTTTAATGAGATTGTCACAAAGCCACTGCTAGAGGGAGCCTTAACTACTTTGAACAATTATCCTGTTCAAGAAGAGGACATTGATGTGGTATGGGTTCCTGGTAGCTTTGAGATTGGTGTAGTTGCACAGAGGCTTGGAAGTTCGGGGAGATATCATGCAGTTTTATGCATTGGAGTTGTGATAAGAGGTGATACAACCCACTACGATGCTGTTGCTAATTCAGCTGCATTCGGAGTGCTTTCAGCTGGTTTAAAATCAGGTGTTCCATGCATATTCGGTGTCTTAACTTGTGATAATATGGAGCAGGCTTTAAATCGAGGTGGTGGTAAATCTGGAAATAAGGGTGCAGAGACTACTTTGACAGCTGTAAGTTTGGACCAACTAGTTTCTATGCTTTGTTCTGACTATAGCTTAATTAAACATTGGCTTAATTAA

Protein Analysis

215

Amino Acids

23.04

Weight (kDa)

8.63

Isoelectric Point (pI)

36.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 62 - 196 4.5e-46 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 14
AciI CCGC 3 cut(s) 65, 83, 143
AfaI GTAC 1 cut(s) 97
AflIII ACRYGT 1 cut(s) 12
AgsI TTSAA 2 cut(s) 265, 281
AhdI GACNNNNNGTC 1 cut(s) 13
AhlI ACTAGT 1 cut(s) 595
AjnI CCWGG 1 cut(s) 310
AloI GAACNNNNNNTCC 2 cut(s) 106, 138
AluBI AGCT 6 cut(s) 179, 318, 437, 458, 578, 624
AluI AGCT 6 cut(s) 179, 318, 437, 458, 578, 624
Alw26I GTCTC 1 cut(s) 557
AoxI GGCC 1 cut(s) 184
ApeKI GCWGC 2 cut(s) 206, 437
AspLEI GCGC 1 cut(s) 33
AspS9I GGNCC 1 cut(s) 589
AsuHPI GGTGA 1 cut(s) 413
AvaII GGWCC 1 cut(s) 589
BanII GRGCYC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 218, 424
BceAI ACGGC 3 cut(s) 44, 78, 199
BcgI CGANNNNNNTGC 2 cut(s) 407, 441
BciT130I CCWGG 1 cut(s) 312
BcoDI GTCTC 1 cut(s) 557
BcuI ACTAGT 1 cut(s) 595
BfaI CTAG 2 cut(s) 242, 596
BfmI CTRYAG 1 cut(s) 619
BfoI RGCGCY 1 cut(s) 34
BisI GCNGC 4 cut(s) 83, 144, 207, 438
BlsI GCNGC 4 cut(s) 84, 145, 208, 439
Bme1390I CCNGG 1 cut(s) 312
Bme18I GGWCC 1 cut(s) 589
BmeRI GACNNNNNGTC 1 cut(s) 13
BmgT120I GGNCC 1 cut(s) 589
BmiI GGNNCC 3 cut(s) 166, 250, 309
BmrFI CCNGG 1 cut(s) 312
BmsI GCATC 2 cut(s) 163, 409
BplI GAGNNNNNCTC 2 cut(s) 110, 142
BpuEI CTTGAG 1 cut(s) 34
BseBI CCWGG 1 cut(s) 312
BseXI GCAGC 2 cut(s) 218, 424
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 186
BsmAI GTCTC 1 cut(s) 557
BsmI GAATGC 1 cut(s) 440
BsnI GGCC 1 cut(s) 186
Bsp1286I GDGCHC 1 cut(s) 23
BspACI CCGC 3 cut(s) 65, 83, 143
BspANI GGCC 1 cut(s) 186
BspFNI CGCG 1 cut(s) 14
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 3 cut(s) 166, 250, 309
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 100, 213
Bst6I CTCTTC 1 cut(s) 279
BstAPI GCANNNNNTGC 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 519
BstFNI CGCG 1 cut(s) 14
BstH2I RGCGCY 1 cut(s) 34
BstHHI GCGC 1 cut(s) 33
BstMAI GTCTC 1 cut(s) 557
BstMWI GCNNNNNNNGC 3 cut(s) 140, 203, 434
BstNI CCWGG 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 310
BstSFI CTRYAG 1 cut(s) 619
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 2 cut(s) 218, 424
BsuRI GGCC 1 cut(s) 186
BtsI GCAGTG 2 cut(s) 204, 236
BtsIMutI CAGTG 3 cut(s) 105, 204, 236
Cac8I GCNNGC 1 cut(s) 519
CciI TCATGA 1 cut(s) 106
CfoI GCGC 1 cut(s) 33
Cfr13I GGNCC 1 cut(s) 589
CseI GACGC 1 cut(s) 3
Csp6I GTAC 1 cut(s) 96
CviAII CATG 3 cut(s) 107, 368, 480
CviQI GTAC 1 cut(s) 96
DraI TTTAAA 2 cut(s) 465, 525
DriI GACNNNNNGTC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 279
Eam1105I GACNNNNNGTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 279
Eco24I GRGCYC 1 cut(s) 23
Eco32I GATATC 1 cut(s) 365
Eco47I GGWCC 1 cut(s) 589
EcoRII CCWGG 1 cut(s) 310
EcoRV GATATC 1 cut(s) 365
EcoT22I ATGCAT 2 cut(s) 383, 485
EcoT38I GRGCYC 1 cut(s) 23
FaeI CATG 3 cut(s) 110, 371, 483
FatI CATG 3 cut(s) 106, 367, 479
Fnu4HI GCNGC 4 cut(s) 83, 144, 207, 438
FriOI GRGCYC 1 cut(s) 23
Fsp4HI GCNGC 4 cut(s) 83, 144, 207, 438
FspBI CTAG 2 cut(s) 242, 596
GlaI GCGC 1 cut(s) 32
GluI GCNGC 4 cut(s) 83, 144, 207, 438
HaeII RGCGCY 1 cut(s) 34
HaeIII GGCC 1 cut(s) 186
HgaI GACGC 1 cut(s) 3
HhaI GCGC 1 cut(s) 33
Hin1II CATG 3 cut(s) 110, 371, 483
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HinfI GANTC 2 cut(s) 73, 103
HphI GGTGA 1 cut(s) 413
Hpy188I TCNGA 3 cut(s) 78, 446, 616
Hpy188III TCNNGA 3 cut(s) 107, 281, 546
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 2 cut(s) 109, 197
HpyCH4III ACNGT 2 cut(s) 100, 213
HpyCH4V TGCA 8 cut(s) 60, 206, 338, 371, 381, 440, 483, 560
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 203, 434
Hsp92II CATG 3 cut(s) 110, 371, 483
HspAI GCGC 1 cut(s) 31
LmnI GCTCC 3 cut(s) 118, 248, 514
LpnPI CCDG 8 cut(s) 143, 288, 297, 324, 444, 456, 503, 531
Lsp1109I GCAGC 2 cut(s) 218, 424
LweI GCATC 2 cut(s) 163, 409
MaeI CTAG 2 cut(s) 242, 596
MaeIII GTNAC 1 cut(s) 226
MboII GAAGA 1 cut(s) 296
MhlI GDGCHC 1 cut(s) 23
MluCI AATT 4 cut(s) 268, 430, 627, 643
MluI ACGCGT 1 cut(s) 12
MlyI GAGTC 2 cut(s) 82, 112
MnlI CCTC 8 cut(s) 58, 105, 136, 238, 280, 336, 392, 524
Mph1103I ATGCAT 2 cut(s) 383, 485
MslI CAYNNNNRTG 2 cut(s) 296, 417
MspA1I CMGCKG 3 cut(s) 437, 458, 578
MspR9I CCNGG 1 cut(s) 312
Mva1269I GAATGC 1 cut(s) 440
MvaI CCWGG 1 cut(s) 312
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 3 cut(s) 140, 203, 434
NlaIII CATG 3 cut(s) 110, 371, 483
NlaIV GGNNCC 3 cut(s) 166, 250, 309
NmuCI GTSAC 1 cut(s) 226
NsiI ATGCAT 2 cut(s) 383, 485
PacI TTAATTAA 2 cut(s) 630, 646
PagI TCATGA 1 cut(s) 106
PctI GAATGC 1 cut(s) 440
PkrI GCNGC 4 cut(s) 84, 145, 208, 439
PleI GAGTC 2 cut(s) 81, 111
PpsI GAGTC 2 cut(s) 81, 111
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PspN4I GGNNCC 3 cut(s) 166, 250, 309
PspPI GGNCC 1 cut(s) 589
PvuII CAGCTG 3 cut(s) 437, 458, 578
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 2 cut(s) 296, 417
SatI GCNGC 4 cut(s) 83, 144, 207, 438
Sau96I GGNCC 1 cut(s) 589
SchI GAGTC 2 cut(s) 82, 112
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 1 cut(s) 23
SfaNI GCATC 2 cut(s) 163, 409
SfcI CTRYAG 1 cut(s) 619
SinI GGWCC 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 296, 417
SmlI CTYRAG 1 cut(s) 49
SmoI CTYRAG 1 cut(s) 49
SpeI ACTAGT 1 cut(s) 595
Sse9I AATT 4 cut(s) 268, 430, 627, 643
SsiI CCGC 3 cut(s) 65, 83, 143
SspMI CTAG 2 cut(s) 242, 596
StyD4I CCNGG 1 cut(s) 310
TaaI ACNGT 2 cut(s) 100, 213
TaqI TCGA 3 cut(s) 17, 136, 529
TasI AATT 4 cut(s) 268, 430, 627, 643
TatI WGTACW 1 cut(s) 95
TauI GCSGC 2 cut(s) 85, 146
TscAI CASTG 3 cut(s) 105, 204, 243
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 2 cut(s) 206, 437
Tsp45I GTSAC 1 cut(s) 226
TspRI CASTG 3 cut(s) 105, 204, 243
VpaK11BI GGWCC 1 cut(s) 589
XspI CTAG 2 cut(s) 242, 596
Zsp2I ATGCAT 2 cut(s) 383, 485
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.