Rroxscaffold_2G00156120

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0008755
Physical Location & Seq
Forward (+)
92573906 .. 92576638
2733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00156120.1

Sequence Viewer

Length: 687 bp
ATGGCTTCTGCTGCTTCGGCTTCAAGCACTGGGTGCTTAGTTCGGTCCCGCCTCCCAGTCGGCGAATGTCAGCATCATCGTAATAACGGGATTGTACTGCTTCCCCAGTTACCCAAACCCCTCATCTCTATTTCTTCTTCTTCTTCTTCCAAAGCTGCTGCAATTCCAACTGCAAGTGAGAGGAAGGAGCGTTCCTCTTTTGCTCGAACTGCGGCTGTTCGGCATCTCGTGGGTTCCGTTACCACAGCTCACGGCCTTCGCTTCGCAGTGGTTGTAGCACGGTTTAATGAGATTATCACCAAGCCACTGCTAGAGGGAGCTTTAACTACTTTCAACAATTATTCTGTTCAAGAAGAGGACATTGATGTGGTATGGGTTCCTGGTAGCTTTGAAATTGGTGTAGTTGCACAGAGGCTTGGAAGTTCAGGGAGATATCATGCAGTTTTATGCATAGGAGCTGTGATAAGAGGTGATACAACCCACTATGATGCTGTTGCTAATTCAGCTGCATCCGGAGTGCTTTCAGCTGGTTTAAAATCAGGTGTTCCATGCATATTTGGTGTCTTAACTTGTGATAATATGGAGCAGGCTTTAAATCGAGCTGGTGGTAAATCTGGAAATAAGGGTGCAGAGACTGCCTTGACAGCTATTGAGATGGCATCTTTGTTTGTGCACCAATTGAAGTAA

Protein Analysis

228

Amino Acids

23.8

Weight (kDa)

9.1

Isoelectric Point (pI)

38.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 85 - 222 5.5e-51 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 512
AciI CCGC 2 cut(s) 49, 212
AdeI CACNNNGTG 1 cut(s) 33
AfaI GTAC 1 cut(s) 96
AgsI TTSAA 5 cut(s) 24, 334, 350, 392, 682
AjnI CCWGG 1 cut(s) 379
AloI GAACNNNNNNTCC 2 cut(s) 175, 207
AluBI AGCT 9 cut(s) 155, 248, 320, 387, 458, 506, 527, 602, 647
AluI AGCT 9 cut(s) 155, 248, 320, 387, 458, 506, 527, 602, 647
Alw21I GWGCWC 1 cut(s) 675
Alw26I GTCTC 1 cut(s) 626
Alw44I GTGCAC 1 cut(s) 671
AlwNI CAGNNNCTG 1 cut(s) 635
Aor13HI TCCGGA 1 cut(s) 512
AoxI GGCC 1 cut(s) 253
ApaLI GTGCAC 1 cut(s) 671
ApeKI GCWGC 4 cut(s) 11, 155, 158, 506
AspS9I GGNCC 1 cut(s) 45
AsuHPI GGTGA 2 cut(s) 289, 482
AvaII GGWCC 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 675
BauI CACGAG 1 cut(s) 227
Bbv12I GWGCWC 1 cut(s) 675
BbvI GCAGC 3 cut(s) 142, 145, 493
BccI CCATC 1 cut(s) 649
BceAI ACGGC 1 cut(s) 268
BciT130I CCWGG 1 cut(s) 381
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 1 cut(s) 311
BisI GCNGC 5 cut(s) 12, 156, 159, 213, 507
BlsI GCNGC 5 cut(s) 13, 157, 160, 214, 508
Bme1390I CCNGG 1 cut(s) 381
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 3 cut(s) 47, 235, 378
BmrFI CCNGG 1 cut(s) 381
BmrI ACTGGG 3 cut(s) 39, 50, 100
BmsI GCATC 5 cut(s) 82, 232, 478, 518, 668
BmuI ACTGGG 3 cut(s) 39, 50, 100
BplI GAGNNNNNCTC 2 cut(s) 179, 211
BsaWI WCCGGW 1 cut(s) 512
Bse1I ACTGG 3 cut(s) 34, 56, 106
BseAI TCCGGA 1 cut(s) 512
BseBI CCWGG 1 cut(s) 381
BseGI GGATG 1 cut(s) 509
BseNI ACTGG 3 cut(s) 34, 56, 106
BseSI GKGCMC 1 cut(s) 675
BseXI GCAGC 3 cut(s) 142, 145, 493
BsgI GTGCAG 1 cut(s) 648
BshFI GGCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 675
BsiSI CCGG 1 cut(s) 513
BslFI GGGAC 1 cut(s) 31
BsmAI GTCTC 1 cut(s) 626
BsmFI GGGAC 1 cut(s) 31
BsnI GGCC 1 cut(s) 255
Bsp1286I GDGCHC 1 cut(s) 675
Bsp13I TCCGGA 1 cut(s) 512
BspACI CCGC 2 cut(s) 49, 212
BspANI GGCC 1 cut(s) 255
BspEI TCCGGA 1 cut(s) 512
BspLI GGNNCC 3 cut(s) 47, 235, 378
BsrI ACTGG 3 cut(s) 34, 56, 106
BssSI CACGAG 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 1 cut(s) 282
Bst6I CTCTTC 1 cut(s) 348
BstAPI GCANNNNNTGC 2 cut(s) 33, 635
BstC8I GCNNGC 1 cut(s) 588
BstDEI CTNAG 1 cut(s) 37
BstF5I GGATG 1 cut(s) 509
BstMAI GTCTC 1 cut(s) 626
BstMWI GCNNNNNNNGC 7 cut(s) 11, 17, 33, 209, 503, 635, 644
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 379
BstSLI GKGCMC 1 cut(s) 675
BstV1I GCAGC 3 cut(s) 142, 145, 493
BsuRI GGCC 1 cut(s) 255
BtsCI GGATG 1 cut(s) 509
BtsI GCAGTG 2 cut(s) 273, 305
BtsIMutI CAGTG 3 cut(s) 27, 273, 305
Cac8I GCNNGC 1 cut(s) 588
CaiI CAGNNNCTG 1 cut(s) 635
Cfr13I GGNCC 1 cut(s) 45
Csp6I GTAC 1 cut(s) 95
CviAII CATG 2 cut(s) 437, 549
CviQI GTAC 1 cut(s) 95
DdeI CTNAG 1 cut(s) 37
DraI TTTAAA 2 cut(s) 534, 594
DraIII CACNNNGTG 1 cut(s) 33
Eam1104I CTCTTC 1 cut(s) 348
EarI CTCTTC 1 cut(s) 348
Eco32I GATATC 1 cut(s) 434
Eco47I GGWCC 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 379
EcoRV GATATC 1 cut(s) 434
EcoT22I ATGCAT 2 cut(s) 452, 554
FaeI CATG 2 cut(s) 440, 552
FaiI YATR 8 cut(s) 373, 438, 448, 452, 486, 550, 554, 581
FaqI GGGAC 1 cut(s) 31
FatI CATG 2 cut(s) 436, 548
FauI CCCGC 1 cut(s) 56
Fnu4HI GCNGC 5 cut(s) 12, 156, 159, 213, 507
FokI GGATG 1 cut(s) 496
Fsp4HI GCNGC 5 cut(s) 12, 156, 159, 213, 507
FspBI CTAG 1 cut(s) 311
GluI GCNGC 5 cut(s) 12, 156, 159, 213, 507
HaeIII GGCC 1 cut(s) 255
HapII CCGG 1 cut(s) 513
Hin1II CATG 2 cut(s) 440, 552
HpaII CCGG 1 cut(s) 513
HphI GGTGA 2 cut(s) 289, 482
Hpy166II GTNNAC 1 cut(s) 673
Hpy188III TCNNGA 3 cut(s) 350, 513, 615
Hpy8I GTNNAC 1 cut(s) 673
HpyAV CCTTC 2 cut(s) 178, 266
HpyCH4III ACNGT 1 cut(s) 282
HpyCH4V TGCA 9 cut(s) 161, 173, 407, 440, 450, 509, 552, 629, 673
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 17, 33, 209, 503, 635, 644
HpyF3I CTNAG 1 cut(s) 37
Hsp92II CATG 2 cut(s) 440, 552
Kpn2I TCCGGA 1 cut(s) 512
LmnI GCTCC 4 cut(s) 187, 317, 455, 583
Lsp1109I GCAGC 3 cut(s) 142, 145, 493
LweI GCATC 5 cut(s) 82, 232, 478, 518, 668
MaeI CTAG 1 cut(s) 311
MaeIII GTNAC 2 cut(s) 108, 238
MboII GAAGA 6 cut(s) 126, 129, 132, 135, 138, 365
MfeI CAATTG 1 cut(s) 677
MhlI GDGCHC 1 cut(s) 675
MluCI AATT 5 cut(s) 162, 337, 393, 499, 677
MmeI TCCRAC 1 cut(s) 191
MnlI CCTC 8 cut(s) 62, 131, 174, 205, 307, 349, 405, 461
Mph1103I ATGCAT 2 cut(s) 452, 554
MroI TCCGGA 1 cut(s) 512
MseI TTAA 5 cut(s) 285, 323, 533, 566, 593
MslI CAYNNNNRTG 2 cut(s) 365, 486
MspA1I CMGCKG 2 cut(s) 506, 527
MspI CCGG 1 cut(s) 513
MspR9I CCNGG 1 cut(s) 381
MunI CAATTG 1 cut(s) 677
MvaI CCWGG 1 cut(s) 381
MwoI GCNNNNNNNGC 7 cut(s) 11, 17, 33, 209, 503, 635, 644
NlaIII CATG 2 cut(s) 440, 552
NlaIV GGNNCC 3 cut(s) 47, 235, 378
NsiI ATGCAT 2 cut(s) 452, 554
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PkrI GCNGC 5 cut(s) 13, 157, 160, 214, 508
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PspN4I GGNNCC 3 cut(s) 47, 235, 378
PspPI GGNCC 1 cut(s) 45
PstNI CAGNNNCTG 1 cut(s) 635
PvuII CAGCTG 2 cut(s) 506, 527
RsaI GTAC 1 cut(s) 96
RsaNI GTAC 1 cut(s) 95
RseI CAYNNNNRTG 2 cut(s) 365, 486
SaqAI TTAA 5 cut(s) 285, 323, 533, 566, 593
SatI GCNGC 5 cut(s) 12, 156, 159, 213, 507
Sau96I GGNCC 1 cut(s) 45
ScrFI CCNGG 1 cut(s) 381
SduI GDGCHC 1 cut(s) 675
SfaNI GCATC 5 cut(s) 82, 232, 478, 518, 668
SinI GGWCC 1 cut(s) 45
SmiMI CAYNNNNRTG 2 cut(s) 365, 486
Sse9I AATT 5 cut(s) 162, 337, 393, 499, 677
SsiI CCGC 2 cut(s) 49, 212
SspMI CTAG 1 cut(s) 311
StyD4I CCNGG 1 cut(s) 379
TaaI ACNGT 1 cut(s) 282
TaqI TCGA 2 cut(s) 205, 598
TaqII GACCGA 1 cut(s) 33
TasI AATT 5 cut(s) 162, 337, 393, 499, 677
TatI WGTACW 1 cut(s) 94
TauI GCSGC 1 cut(s) 215
Tru1I TTAA 5 cut(s) 285, 323, 533, 566, 593
Tru9I TTAA 5 cut(s) 285, 323, 533, 566, 593
TscAI CASTG 3 cut(s) 34, 273, 312
TseI GCWGC 4 cut(s) 11, 155, 158, 506
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 3 cut(s) 34, 273, 312
VneI GTGCAC 1 cut(s) 671
VpaK11BI GGWCC 1 cut(s) 45
XspI CTAG 1 cut(s) 311
Zsp2I ATGCAT 2 cut(s) 452, 554
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.