FvH4_1g00110

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0008755
Physical Location & Seq
Reverse (-)
71489 .. 75981
4493 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00110.t1

Sequence Viewer

Length: 657 bp
ATGGCTTCTGCTTCAGCTTCCGCTTCAACCCGGTGGTTCGTTCAGTCACGGCTCCCTCCTCCTCGTAATAACGGGCTGCACCGGTTACCCAATACCCTCCTCTCTCTTTCTTCTTTTTCTTCTTCCAAAGTTGCAATTGCAATTTCAAATCCAACTGAGAGGAAGAGGTTTGCTCAAACCGCGGCTGTTCGGCATCTCGTGGGTTCCGTTACCACCGCTCACGGCCTTCGTTTTGCAGTCGTTGTTGCACGGTTTAATGAGATTATCACAAAGCCACTGCTAGAGGGAGCTTTGACTACTTTCCACAATTATTCTGTTCAAGAAGAAGACATTGATGTGGTCTGGGTTCCTGGTAGCTTTGAAATTGGTGTAGTTGCACAAAAGCTTGGAAGTTCGGGGAGATATCATGCTGTTTTATGCATTGGAGCTGTGATAAGAGGTGATACAACCCACTATGATGCTGTTGCTAATTCAGCAGCTTCTGGAGTACTTTCAGCTGGCTTAAAGTCAGGTGTTCCATGCATCTTTGGTGTGTTGACTTGTGATAATATGGAGCAGGCTTTAAATCGAGCTGGTGGTAAATCCGGAAATAAGGGGGCGGAGGCTGCCTTGACAGCTATTGAGATGGCGTCTTTGTTTGTACACCAATTGAAGTAG

Protein Analysis

219

Amino Acids

23.0

Weight (kDa)

9.51

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 75 - 212 8.1e-52 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 218
AccII CGCG 1 cut(s) 182
AccIII TCCGGA 1 cut(s) 584
AciI CCGC 5 cut(s) 21, 180, 182, 216, 599
AcyI GRCGYC 1 cut(s) 629
AfaI GTAC 2 cut(s) 489, 642
AgeI ACCGGT 1 cut(s) 81
AgsI TTSAA 5 cut(s) 27, 147, 320, 362, 652
AjnI CCWGG 1 cut(s) 349
AluBI AGCT 9 cut(s) 17, 290, 357, 385, 428, 479, 497, 572, 617
AluI AGCT 9 cut(s) 17, 290, 357, 385, 428, 479, 497, 572, 617
AlwNI CAGNNNCTG 1 cut(s) 482
Aor13HI TCCGGA 1 cut(s) 584
AoxI GGCC 1 cut(s) 223
ApeKI GCWGC 3 cut(s) 76, 476, 605
AsiGI ACCGGT 1 cut(s) 81
AsuC2I CCSGG 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 452
BauI CACGAG 1 cut(s) 197
BbsI GAAGAC 1 cut(s) 333
BbvI GCAGC 3 cut(s) 63, 488, 592
BccI CCATC 1 cut(s) 619
BceAI ACGGC 2 cut(s) 65, 238
BciT130I CCWGG 1 cut(s) 351
BcnI CCSGG 1 cut(s) 31
BfaI CTAG 1 cut(s) 281
BisI GCNGC 4 cut(s) 77, 183, 477, 606
BlsI GCNGC 4 cut(s) 78, 184, 478, 607
BmcAI AGTACT 1 cut(s) 489
Bme1390I CCNGG 2 cut(s) 31, 351
BmiI GGNNCC 3 cut(s) 53, 205, 348
BmrFI CCNGG 2 cut(s) 31, 351
BmsI GCATC 3 cut(s) 202, 448, 531
BpiI GAAGAC 1 cut(s) 333
BplI GAGNNNNNCTC 2 cut(s) 157, 189
BpmI CTGGAG 1 cut(s) 504
BpuMI CCSGG 1 cut(s) 31
BsaHI GRCGYC 1 cut(s) 629
BsaJI CCNNGG 1 cut(s) 180
BsaWI WCCGGW 2 cut(s) 81, 584
Bse118I RCCGGY 1 cut(s) 81
BseAI TCCGGA 1 cut(s) 584
BseBI CCWGG 1 cut(s) 351
BseDI CCNNGG 1 cut(s) 180
BseMII CTCAG 1 cut(s) 147
BseRI GAGGAG 3 cut(s) 48, 51, 89
BseXI GCAGC 3 cut(s) 63, 488, 592
BsgI GTGCAG 1 cut(s) 62
Bsh1236I CGCG 1 cut(s) 182
BshFI GGCC 1 cut(s) 225
BshTI ACCGGT 1 cut(s) 81
BsiSI CCGG 3 cut(s) 31, 82, 585
BsnI GGCC 1 cut(s) 225
Bsp13I TCCGGA 1 cut(s) 584
Bsp1407I TGTACA 1 cut(s) 640
BspACI CCGC 5 cut(s) 21, 180, 182, 216, 599
BspANI GGCC 1 cut(s) 225
BspCNI CTCAG 1 cut(s) 148
BspEI TCCGGA 1 cut(s) 584
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 3 cut(s) 53, 205, 348
BsrBI CCGCTC 1 cut(s) 218
BsrFI RCCGGY 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 640
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 1 cut(s) 180
BssNI GRCGYC 1 cut(s) 629
BssSI CACGAG 1 cut(s) 197
Bst2BI CACGAG 1 cut(s) 197
Bst2UI CCWGG 1 cut(s) 351
Bst4CI ACNGT 1 cut(s) 252
Bst6I CTCTTC 1 cut(s) 158
BstACI GRCGYC 1 cut(s) 629
BstAUI TGTACA 1 cut(s) 640
BstC8I GCNNGC 2 cut(s) 499, 558
BstDEI CTNAG 1 cut(s) 156
BstDSI CCRYGG 1 cut(s) 180
BstEII GGTNACC 1 cut(s) 84
BstFNI CGCG 1 cut(s) 182
BstMWI GCNNNNNNNGC 4 cut(s) 179, 473, 605, 614
BstNI CCWGG 1 cut(s) 351
BstPI GGTNACC 1 cut(s) 84
BstSCI CCNGG 2 cut(s) 29, 349
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 3 cut(s) 63, 488, 592
BstV2I GAAGAC 1 cut(s) 333
BsuRI GGCC 1 cut(s) 225
BtgI CCRYGG 1 cut(s) 180
BtsI GCAGTG 1 cut(s) 275
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 2 cut(s) 499, 558
CaiI CAGNNNCTG 1 cut(s) 482
Cfr10I RCCGGY 1 cut(s) 81
Cfr42I CCGCGG 1 cut(s) 183
CseI GACGC 1 cut(s) 618
Csp6I GTAC 2 cut(s) 488, 641
CspAI ACCGGT 1 cut(s) 81
CviAII CATG 2 cut(s) 407, 519
CviQI GTAC 2 cut(s) 488, 641
DdeI CTNAG 1 cut(s) 156
DraI TTTAAA 1 cut(s) 564
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
EciI GGCGGA 1 cut(s) 614
Eco32I GATATC 1 cut(s) 404
Eco91I GGTNACC 1 cut(s) 84
EcoO65I GGTNACC 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 349
EcoRV GATATC 1 cut(s) 404
EcoT22I ATGCAT 2 cut(s) 422, 524
FaeI CATG 2 cut(s) 410, 522
FaiI YATR 5 cut(s) 408, 418, 456, 520, 551
FatI CATG 2 cut(s) 406, 518
Fnu4HI GCNGC 4 cut(s) 77, 183, 477, 606
Fsp4HI GCNGC 4 cut(s) 77, 183, 477, 606
FspBI CTAG 1 cut(s) 281
GluI GCNGC 4 cut(s) 77, 183, 477, 606
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 1 cut(s) 225
HapII CCGG 3 cut(s) 31, 82, 585
HgaI GACGC 1 cut(s) 618
Hin1I GRCGYC 1 cut(s) 629
Hin1II CATG 2 cut(s) 410, 522
HincII GTYRAC 1 cut(s) 537
HindII GTYRAC 1 cut(s) 537
HindIII AAGCTT 1 cut(s) 383
HpaII CCGG 3 cut(s) 31, 82, 585
HphI GGTGA 1 cut(s) 452
Hpy166II GTNNAC 2 cut(s) 537, 643
Hpy188III TCNNGA 3 cut(s) 320, 483, 585
Hpy8I GTNNAC 2 cut(s) 537, 643
HpyAV CCTTC 1 cut(s) 236
HpyCH4III ACNGT 1 cut(s) 252
HpyCH4V TGCA 8 cut(s) 79, 134, 140, 236, 248, 377, 420, 522
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 473, 605, 614
HpyF3I CTNAG 1 cut(s) 156
Hsp92I GRCGYC 1 cut(s) 629
Hsp92II CATG 2 cut(s) 410, 522
Kpn2I TCCGGA 1 cut(s) 584
KspI CCGCGG 1 cut(s) 183
LmnI GCTCC 4 cut(s) 57, 287, 425, 553
Lsp1109I GCAGC 3 cut(s) 63, 488, 592
LweI GCATC 3 cut(s) 202, 448, 531
MaeI CTAG 1 cut(s) 281
MaeIII GTNAC 3 cut(s) 45, 84, 208
MbiI CCGCTC 1 cut(s) 218
MboII GAAGA 6 cut(s) 102, 111, 114, 175, 335, 338
MfeI CAATTG 2 cut(s) 135, 647
MluCI AATT 6 cut(s) 135, 141, 307, 363, 469, 647
MmeI TCCRAC 1 cut(s) 176
Mph1103I ATGCAT 2 cut(s) 422, 524
MroI TCCGGA 1 cut(s) 584
MseI TTAA 3 cut(s) 255, 503, 563
MslI CAYNNNNRTG 2 cut(s) 335, 456
MspA1I CMGCKG 2 cut(s) 182, 497
MspI CCGG 3 cut(s) 31, 82, 585
MspR9I CCNGG 2 cut(s) 31, 351
MunI CAATTG 2 cut(s) 135, 647
MvaI CCWGG 1 cut(s) 351
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 4 cut(s) 179, 473, 605, 614
NciI CCSGG 1 cut(s) 31
NlaIII CATG 2 cut(s) 410, 522
NlaIV GGNNCC 3 cut(s) 53, 205, 348
NmuCI GTSAC 1 cut(s) 45
NsiI ATGCAT 2 cut(s) 422, 524
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PinAI ACCGGT 1 cut(s) 81
PkrI GCNGC 4 cut(s) 78, 184, 478, 607
Psp6I CCWGG 1 cut(s) 349
PspEI GGTNACC 1 cut(s) 84
PspGI CCWGG 1 cut(s) 349
PspN4I GGNNCC 3 cut(s) 53, 205, 348
PstNI CAGNNNCTG 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 497
RsaI GTAC 2 cut(s) 489, 642
RsaNI GTAC 2 cut(s) 488, 641
RseI CAYNNNNRTG 2 cut(s) 335, 456
SacII CCGCGG 1 cut(s) 183
SaqAI TTAA 3 cut(s) 255, 503, 563
SatI GCNGC 4 cut(s) 77, 183, 477, 606
ScaI AGTACT 1 cut(s) 489
ScrFI CCNGG 2 cut(s) 31, 351
SfaNI GCATC 3 cut(s) 202, 448, 531
Sfr303I CCGCGG 1 cut(s) 183
SgrBI CCGCGG 1 cut(s) 183
SmiMI CAYNNNNRTG 2 cut(s) 335, 456
Sse9I AATT 6 cut(s) 135, 141, 307, 363, 469, 647
SsiI CCGC 5 cut(s) 21, 180, 182, 216, 599
SspMI CTAG 1 cut(s) 281
StyD4I CCNGG 2 cut(s) 29, 349
TaaI ACNGT 1 cut(s) 252
TaqI TCGA 1 cut(s) 568
TasI AATT 6 cut(s) 135, 141, 307, 363, 469, 647
TatI WGTACW 2 cut(s) 487, 640
TauI GCSGC 1 cut(s) 185
Tru1I TTAA 3 cut(s) 255, 503, 563
Tru9I TTAA 3 cut(s) 255, 503, 563
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 45
TseI GCWGC 3 cut(s) 76, 476, 605
Tsp45I GTSAC 1 cut(s) 45
TspGWI ACGGA 1 cut(s) 196
TspRI CASTG 1 cut(s) 282
XspI CTAG 1 cut(s) 281
ZrmI AGTACT 1 cut(s) 489
Zsp2I ATGCAT 2 cut(s) 422, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.