FvH4_2g10600

Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0008755
Physical Location & Seq
Forward (+)
9380892 .. 9385745
4854 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g10600.t1

Sequence Viewer

Length: 663 bp
ATGGCTTCTGCTTCCGCTTCAACCCAGTGCTTTGTTCAGTCACGCTTCCCATTCAGGGAATCTCATCATCATAATAACAGGATTGTACAGCTTCACCAGTTACCAAAAACCCTGCTCTCTCTTTCTTCTGCCAAAGCTGCTGCATTTGCAATTGAGAGGAAGGAGCATTCCTGTTTTGCGCAAACTGCGGCTGTTCGCCATCTCGTGGGTTCCGTTACCACTGCTCACGGCCTTCGTTTCGCAGTGGTTGTAGCGCGATTTAATGAGATTATCACAAAGCCACTGCTAGAGGGAGCTTTAGCTACTTTCAAGGATTATTCAGTTCAAGAAGAGGACATTGATGTGGTATGGGTTCCTGGTAGCTTTGAAATTGGTGTAGTTGCACAGAGGCTTGGAAGTTCGGGGAGATATGATGCAGTTTTATGCATTGGAGCTGTGATAAGAGGTGATACAACCCACTATGATGCTGTTGCAAATTCAGCGGCATCTGGAGTACTATCAGCTGGTTTAAAATCAGGTGTTCCATGCATATTTGGTGTCTTAACTTGTGATAATATGGAGCAGGCTTTAAACCGAGCTGGTGGTAAATCTGGAAATAAGGGTGCGGAGACTGCCTTGACAGCTATTGAAATGGCTTCTCTGTTTGTGCACCAATTGAAGTAG

Protein Analysis

221

Amino Acids

23.41

Weight (kDa)

8.31

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DMRL_synthase PF00885 77 - 214 2.2e-52 6,7-dimethyl-8-ribityllumazine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 180
AccB7I CCANNNNNTGG 1 cut(s) 205
AccII CGCG 1 cut(s) 256
AciI CCGC 4 cut(s) 15, 188, 482, 605
AcsI RAATTY 1 cut(s) 475
AfaI GTAC 2 cut(s) 87, 495
AfiI CCNNNNNNNGG 2 cut(s) 55, 205
AgsI TTSAA 6 cut(s) 21, 310, 326, 368, 629, 658
AjnI CCWGG 1 cut(s) 355
AluBI AGCT 9 cut(s) 91, 137, 296, 302, 363, 434, 503, 578, 623
AluI AGCT 9 cut(s) 91, 137, 296, 302, 363, 434, 503, 578, 623
Alw21I GWGCWC 1 cut(s) 651
Alw26I GTCTC 1 cut(s) 602
Alw44I GTGCAC 1 cut(s) 647
AoxI GGCC 1 cut(s) 229
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 2 cut(s) 137, 140
ApoI RAATTY 1 cut(s) 475
AspLEI GCGC 2 cut(s) 181, 256
AsuHPI GGTGA 2 cut(s) 86, 458
BaeGI GKGCMC 1 cut(s) 651
BauI CACGAG 1 cut(s) 203
Bbv12I GWGCWC 1 cut(s) 651
BbvI GCAGC 2 cut(s) 124, 127
BccI CCATC 1 cut(s) 207
BceAI ACGGC 1 cut(s) 244
BciT130I CCWGG 1 cut(s) 357
BcoDI GTCTC 1 cut(s) 602
BfaI CTAG 1 cut(s) 287
BisI GCNGC 4 cut(s) 138, 141, 189, 483
BlsI GCNGC 4 cut(s) 139, 142, 190, 484
BmcAI AGTACT 1 cut(s) 495
Bme1390I CCNGG 1 cut(s) 357
BmiI GGNNCC 2 cut(s) 211, 354
BmrFI CCNGG 1 cut(s) 357
BmrI ACTGGG 1 cut(s) 19
BmsI GCATC 3 cut(s) 403, 454, 494
BmuI ACTGGG 1 cut(s) 19
BpmI CTGGAG 1 cut(s) 510
Bsc4I CCNNNNNNNGG 2 cut(s) 55, 205
Bse1I ACTGG 2 cut(s) 25, 97
BseBI CCWGG 1 cut(s) 357
BseLI CCNNNNNNNGG 2 cut(s) 55, 205
BseNI ACTGG 2 cut(s) 25, 97
BseSI GKGCMC 1 cut(s) 651
BseXI GCAGC 2 cut(s) 124, 127
Bsh1236I CGCG 1 cut(s) 256
BshFI GGCC 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 651
BslI CCNNNNNNNGG 2 cut(s) 55, 205
BsmAI GTCTC 1 cut(s) 602
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 651
Bsp1407I TGTACA 1 cut(s) 85
BspACI CCGC 4 cut(s) 15, 188, 482, 605
BspANI GGCC 1 cut(s) 231
BspFNI CGCG 1 cut(s) 256
BspLI GGNNCC 2 cut(s) 211, 354
BsrGI TGTACA 1 cut(s) 85
BsrI ACTGG 2 cut(s) 25, 97
BssSI CACGAG 1 cut(s) 203
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 357
Bst6I CTCTTC 1 cut(s) 324
BstAUI TGTACA 1 cut(s) 85
BstC8I GCNNGC 1 cut(s) 564
BstFNI CGCG 1 cut(s) 256
BstHHI GCGC 2 cut(s) 181, 256
BstMAI GTCTC 1 cut(s) 602
BstMWI GCNNNNNNNGC 6 cut(s) 137, 146, 185, 479, 611, 620
BstNI CCWGG 1 cut(s) 357
BstSCI CCNGG 1 cut(s) 355
BstSLI GKGCMC 1 cut(s) 651
BstUI CGCG 1 cut(s) 256
BstV1I GCAGC 2 cut(s) 124, 127
BsuRI GGCC 1 cut(s) 231
BtsI GCAGTG 3 cut(s) 219, 249, 281
BtsIMutI CAGTG 4 cut(s) 32, 219, 249, 281
Cac8I GCNNGC 1 cut(s) 564
CfoI GCGC 2 cut(s) 181, 256
Csp6I GTAC 2 cut(s) 86, 494
CviAII CATG 1 cut(s) 525
CviQI GTAC 2 cut(s) 86, 494
DraI TTTAAA 2 cut(s) 510, 570
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
EcoRII CCWGG 1 cut(s) 355
EcoT22I ATGCAT 2 cut(s) 428, 530
FaeI CATG 1 cut(s) 528
FaiI YATR 8 cut(s) 72, 349, 411, 424, 462, 526, 530, 557
FatI CATG 1 cut(s) 524
Fnu4HI GCNGC 4 cut(s) 138, 141, 189, 483
Fsp4HI GCNGC 4 cut(s) 138, 141, 189, 483
FspBI CTAG 1 cut(s) 287
FspI TGCGCA 1 cut(s) 180
GlaI GCGC 2 cut(s) 180, 255
GluI GCNGC 4 cut(s) 138, 141, 189, 483
GsuI CTGGAG 1 cut(s) 510
HaeIII GGCC 1 cut(s) 231
HhaI GCGC 2 cut(s) 181, 256
Hin1II CATG 1 cut(s) 528
Hin6I GCGC 2 cut(s) 179, 254
HinP1I GCGC 2 cut(s) 179, 254
HinfI GANTC 1 cut(s) 59
HphI GGTGA 2 cut(s) 86, 458
Hpy166II GTNNAC 1 cut(s) 649
Hpy188III TCNNGA 3 cut(s) 326, 489, 591
Hpy8I GTNNAC 1 cut(s) 649
HpyAV CCTTC 2 cut(s) 154, 242
HpyCH4V TGCA 8 cut(s) 143, 149, 383, 416, 426, 473, 528, 649
HpyF10VI GCNNNNNNNGC 6 cut(s) 137, 146, 185, 479, 611, 620
Hsp92II CATG 1 cut(s) 528
HspAI GCGC 2 cut(s) 179, 254
LmnI GCTCC 4 cut(s) 163, 293, 431, 559
Lsp1109I GCAGC 2 cut(s) 124, 127
LweI GCATC 3 cut(s) 403, 454, 494
MaeI CTAG 1 cut(s) 287
MaeIII GTNAC 3 cut(s) 39, 99, 214
MboII GAAGA 2 cut(s) 117, 341
MfeI CAATTG 2 cut(s) 150, 653
MhlI GDGCHC 1 cut(s) 651
MluCI AATT 4 cut(s) 150, 369, 475, 653
MnlI CCTC 5 cut(s) 150, 283, 325, 381, 437
Mph1103I ATGCAT 2 cut(s) 428, 530
MseI TTAA 4 cut(s) 261, 509, 542, 569
MslI CAYNNNNRTG 2 cut(s) 341, 462
MspA1I CMGCKG 2 cut(s) 482, 503
MspR9I CCNGG 1 cut(s) 357
MunI CAATTG 2 cut(s) 150, 653
Mva1269I GAATGC 1 cut(s) 166
MvaI CCWGG 1 cut(s) 357
MvnI CGCG 1 cut(s) 256
MwoI GCNNNNNNNGC 6 cut(s) 137, 146, 185, 479, 611, 620
NlaIII CATG 1 cut(s) 528
NlaIV GGNNCC 2 cut(s) 211, 354
NmuCI GTSAC 1 cut(s) 39
NsbI TGCGCA 1 cut(s) 180
NsiI ATGCAT 2 cut(s) 428, 530
PcsI WCGNNNNNNNCGW 1 cut(s) 210
PctI GAATGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 4 cut(s) 139, 142, 190, 484
Psp6I CCWGG 1 cut(s) 355
PspGI CCWGG 1 cut(s) 355
PspN4I GGNNCC 2 cut(s) 211, 354
PvuII CAGCTG 1 cut(s) 503
RsaI GTAC 2 cut(s) 87, 495
RsaNI GTAC 2 cut(s) 86, 494
RseI CAYNNNNRTG 2 cut(s) 341, 462
SaqAI TTAA 4 cut(s) 261, 509, 542, 569
SatI GCNGC 4 cut(s) 138, 141, 189, 483
ScaI AGTACT 1 cut(s) 495
ScrFI CCNGG 1 cut(s) 357
SduI GDGCHC 1 cut(s) 651
SfaNI GCATC 3 cut(s) 403, 454, 494
SmiMI CAYNNNNRTG 2 cut(s) 341, 462
Sse9I AATT 4 cut(s) 150, 369, 475, 653
SsiI CCGC 4 cut(s) 15, 188, 482, 605
SspMI CTAG 1 cut(s) 287
StyD4I CCNGG 1 cut(s) 355
TasI AATT 4 cut(s) 150, 369, 475, 653
TatI WGTACW 2 cut(s) 85, 493
TauI GCSGC 2 cut(s) 191, 485
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 4 cut(s) 261, 509, 542, 569
Tru9I TTAA 4 cut(s) 261, 509, 542, 569
TscAI CASTG 4 cut(s) 32, 226, 249, 288
TseFI GTSAC 1 cut(s) 39
TseI GCWGC 2 cut(s) 137, 140
Tsp45I GTSAC 1 cut(s) 39
TspGWI ACGGA 1 cut(s) 202
TspRI CASTG 4 cut(s) 32, 226, 249, 288
Van91I CCANNNNNTGG 1 cut(s) 205
VneI GTGCAC 1 cut(s) 647
XapI RAATTY 1 cut(s) 475
XspI CTAG 1 cut(s) 287
ZrmI AGTACT 1 cut(s) 495
Zsp2I ATGCAT 2 cut(s) 428, 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.