AT2G44190

endosperm defective

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0005101
Physical Location & Seq
Forward (+)
18272119 .. 18274679
2561 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44190.1

Sequence Viewer

Length: 1425 bp
ATGGAGGCGAGAATCGGCCGATCCATGGAACACCCATCTACACCTGCGATTAATGCTCCGGCGCCGGTTCCTCCTCCTTCTACGAGGCGGCCGAGAGTAAGAGAAGTGAGCTCGAGATTCATGTCTCCCATTTCTTCTTCGTCATCGTCGTCTTCCTCTTCTTCCGCCGGAGATCTCCATCAATTGACTTCAAATTCTCCTAGACATCATCACCAACATCAGAATCAGAGATCGACTTCGGCTCAGAGGATGAGACGGCAATTGAAAATGCAAGAGGGAGACGAGAATCGTCCTTCCGAGACGGCGCGTAGCTTAGACTCGCCTTTTCCTCTTCAACAAGTCGACGGAGGTAAAAACCCTAAGCAGCATATTCGATCTAAGCCGTTGAAAGAGAACGGTCACCGTCTCGACACACCAACGACGGCTATGTTGCCGCCACCGTCTAGATCGCGGCTGAACCAACAGCGTTTACTCACTGCCTCCGCTGCGACTAGGCTTCTCCGATCAAGCGGGATCTCGTTATCATCTTCTACAGACGGTGAAGAAGATAACAACAATAGAGAGATTTTCAAATCCAACGGCCCAGATCTGTTGCCAACAATTAGAACCCAAGCAAAGGCCTTTAACACTCCCACCGCCTCACCACTTTCTCGATCCCTGAGTTCCGATGATGCGTCGATGTTTCGAGATGTTAGAGCTTCATTGTCGCTTAAAAATGGTGTTGGTTTGTCATTGCCTCCGGTGGCTCCTAATTCGAAGATTCAAGCCGATACAAAGAAGCAGAAGAAAGCTTTAGGACAACAAGCGGATGTGCATTCTCTGAAACTGCTCCATAACCGTTACTTGCAATGGCGATTTGCAAATGCAAATGCTGAGGTCAAAACACAATCTCAGAAAGCACAAGCTGAGAGAATGTTTTATTCACTTGGTTTGAAAATGTCGGAGTTGTCTGATTCTGTACAGAGGAAACGCATAGAACTCCAACATTTACAGAGAGTTAAAGCTGTTACGGAGATTGTAGAGTCACAAACTCCTTCTTTGGAGCAATGGGCTGTTCTTGAAGATGAATTCTCAACTTCACTATTGGAAACAACTGAAGCTTTGTTGAATGCTTCATTGCGGTTGCCTCTCGATTCAAAAATCAAGGTTGAGACTAAAGAGTTAGCAGAAGCACTTGTTGTCGCTTCAAAATCTATGGAAGGCATTGTGCAAAACATTGGAAATCTTGTGCCTAAGACACAAGAGATGGAGACTTTGATGTCGGAATTAGCTAGAGTAAGCGGCATAGAGAAAGCATCAGTAGAAGATTGCAGAGTTGCACTGCTTAAGACACATTCATCACAGATGGAAGAATGCTATCTTAGAAGTCAGCTTATTCAACACCAGAAGAAGTGCCATCAGCAAGAGTGCACAACTTCTGTTTGA

Protein Analysis

474

Amino Acids

52.59

Weight (kDa)

9.65

Isoelectric Point (pI)

65.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 80 - 433 3.4e-45 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 52
AasI GACNNNNNNGTC 1 cut(s) 1258
Acc36I ACCTGC 1 cut(s) 52
AccB1I GGYRCC 1 cut(s) 61
AccI GTMKAC 1 cut(s) 342
AccII CGCG 2 cut(s) 307, 451
AclWI GGATC 3 cut(s) 15, 521, 648
AcoI YGGCCR 2 cut(s) 16, 89
AcsI RAATTY 2 cut(s) 193, 1067
AcuI CTGAAG 1 cut(s) 1116
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 1 cut(s) 960
AfiI CCNNNNNNNGG 2 cut(s) 25, 616
AflII CTTAAG 1 cut(s) 1325
AluBI AGCT 9 cut(s) 111, 312, 698, 791, 905, 1004, 1100, 1271, 1372
AluI AGCT 9 cut(s) 111, 312, 698, 791, 905, 1004, 1100, 1271, 1372
Alw21I GWGCWC 2 cut(s) 113, 1412
Alw26I GTCTC 7 cut(s) 129, 247, 273, 293, 410, 1145, 1244
Alw44I GTGCAC 1 cut(s) 1408
AlwI GGATC 3 cut(s) 15, 521, 648
Ama87I CYCGRG 1 cut(s) 112
AoxI GGCC 4 cut(s) 16, 89, 580, 618
ApaLI GTGCAC 1 cut(s) 1408
ApeKI GCWGC 2 cut(s) 364, 485
ApoI RAATTY 2 cut(s) 193, 1067
AseI ATTAAT 1 cut(s) 51
AspLEI GCGC 2 cut(s) 64, 307
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 4 cut(s) 203, 392, 551, 633
AsuII TTCGAA 1 cut(s) 755
AvaI CYCGRG 1 cut(s) 112
BaeGI GKGCMC 1 cut(s) 1412
BanI GGYRCC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 2 cut(s) 113, 1412
BbvCI CCTCAGC 1 cut(s) 873
BbvI GCAGC 2 cut(s) 376, 472
BccI CCATC 5 cut(s) 43, 186, 1240, 1339, 1404
BceAI ACGGC 5 cut(s) 272, 318, 367, 438, 595
BcgI CGANNNNNNTGC 2 cut(s) 353, 387
BcoDI GTCTC 7 cut(s) 129, 247, 273, 293, 410, 1145, 1244
BfaI CTAG 4 cut(s) 201, 444, 492, 1272
BfmI CTRYAG 1 cut(s) 531
BfoI RGCGCY 1 cut(s) 65
BfrI CTTAAG 1 cut(s) 1325
BfuAI ACCTGC 1 cut(s) 52
BglII AGATCT 2 cut(s) 172, 586
BisI GCNGC 6 cut(s) 89, 365, 434, 452, 486, 1282
BlsI GCNGC 6 cut(s) 90, 366, 435, 453, 487, 1283
BmeT110I CYCGRG 1 cut(s) 112
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 3 cut(s) 63, 69, 747
BmsI GCATC 2 cut(s) 661, 1304
BpiI GAAGAC 1 cut(s) 144
Bpu10I CCTNAGC 2 cut(s) 360, 873
Bpu14I TTCGAA 1 cut(s) 755
BsaBI GATNNNNATC 1 cut(s) 177
BsaHI GRCGYC 1 cut(s) 62
BsaJI CCNNGG 1 cut(s) 24
BsaWI WCCGGW 1 cut(s) 739
Bsc4I CCNNNNNNNGG 2 cut(s) 25, 616
Bse118I RCCGGY 1 cut(s) 64
Bse3DI GCAATG 4 cut(s) 731, 854, 1052, 1115
Bse8I GATNNNNATC 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 2 cut(s) 255, 814
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 2 cut(s) 25, 616
BseMI GCAATG 4 cut(s) 731, 854, 1052, 1115
BseMII CTCAG 5 cut(s) 257, 650, 864, 897, 905
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 1412
BseX3I CGGCCG 2 cut(s) 16, 89
BseXI GCAGC 2 cut(s) 376, 472
Bsh1236I CGCG 2 cut(s) 307, 451
Bsh1285I CGRYCG 2 cut(s) 19, 92
BshFI GGCC 4 cut(s) 18, 91, 582, 620
BshNI GGYRCC 1 cut(s) 61
BsiEI CGRYCG 2 cut(s) 19, 92
BsiHKAI GWGCWC 2 cut(s) 113, 1412
BsiHKCI CYCGRG 1 cut(s) 112
BsiSI CCGG 4 cut(s) 59, 65, 168, 740
BslI CCNNNNNNNGG 2 cut(s) 25, 616
BsmAI GTCTC 7 cut(s) 129, 247, 273, 293, 410, 1145, 1244
BsmBI CGTCTC 4 cut(s) 247, 273, 293, 410
BsmI GAATGC 3 cut(s) 814, 1114, 1358
BsnI GGCC 4 cut(s) 18, 91, 582, 620
BsoBI CYCGRG 1 cut(s) 112
Bsp119I TTCGAA 1 cut(s) 755
Bsp1286I GDGCHC 2 cut(s) 113, 1412
Bsp1407I TGTACA 1 cut(s) 958
Bsp143I GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
Bsp19I CCATGG 1 cut(s) 24
BspANI GGCC 4 cut(s) 18, 91, 582, 620
BspCNI CTCAG 5 cut(s) 256, 651, 865, 898, 904
BspFNI CGCG 2 cut(s) 307, 451
BspLI GGNNCC 3 cut(s) 63, 69, 747
BspMI ACCTGC 1 cut(s) 52
BspPI GGATC 3 cut(s) 15, 521, 648
BspT104I TTCGAA 1 cut(s) 755
BspT107I GGYRCC 1 cut(s) 61
BspTI CTTAAG 1 cut(s) 1325
BsrDI GCAATG 4 cut(s) 731, 854, 1052, 1115
BsrFI RCCGGY 1 cut(s) 64
BsrGI TGTACA 1 cut(s) 958
BssAI RCCGGY 1 cut(s) 64
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 24
Bst4CI ACNGT 5 cut(s) 398, 404, 441, 539, 839
Bst6I CTCTTC 2 cut(s) 163, 336
BstACI GRCGYC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 1325
BstAUI TGTACA 1 cut(s) 958
BstBI TTCGAA 1 cut(s) 755
BstDSI CCRYGG 1 cut(s) 24
BstEII GGTNACC 1 cut(s) 398
BstF5I GGATG 2 cut(s) 255, 814
BstFNI CGCG 2 cut(s) 307, 451
BstH2I RGCGCY 1 cut(s) 65
BstHHI GCGC 2 cut(s) 64, 307
BstKTI GATC 9 cut(s) 23, 175, 233, 377, 449, 506, 516, 589, 656
BstMAI GTCTC 7 cut(s) 129, 247, 273, 293, 410, 1145, 1244
BstMBI GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
BstMCI CGRYCG 2 cut(s) 19, 92
BstMWI GCNNNNNNNGC 2 cut(s) 53, 485
BstPI GGTNACC 1 cut(s) 398
BstSFI CTRYAG 1 cut(s) 531
BstSLI GKGCMC 1 cut(s) 1412
BstUI CGCG 2 cut(s) 307, 451
BstV1I GCAGC 2 cut(s) 376, 472
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 3 cut(s) 172, 513, 586
BstYI RGATCY 3 cut(s) 172, 513, 586
BstZI CGGCCG 2 cut(s) 16, 89
BsuRI GGCC 4 cut(s) 18, 91, 582, 620
BtgI CCRYGG 1 cut(s) 24
BtsCI GGATG 2 cut(s) 255, 814
BtsI GCAGTG 2 cut(s) 474, 1319
BtsIMutI CAGTG 2 cut(s) 474, 1319
BveI ACCTGC 1 cut(s) 52
CfoI GCGC 2 cut(s) 64, 307
Cfr10I RCCGGY 1 cut(s) 64
Cfr13I GGNCC 1 cut(s) 581
CseI GACGC 1 cut(s) 663
Csp6I GTAC 1 cut(s) 959
CviAII CATG 2 cut(s) 25, 121
CviQI GTAC 1 cut(s) 959
DinI GGCGCC 1 cut(s) 63
DpnI GATC 9 cut(s) 22, 174, 232, 376, 448, 505, 515, 588, 655
DpnII GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
DrdI GACNNNNNNGTC 1 cut(s) 1258
DseDI GACNNNNNNGTC 1 cut(s) 1258
EaeI YGGCCR 2 cut(s) 16, 89
EagI CGGCCG 2 cut(s) 16, 89
Eam1104I CTCTTC 2 cut(s) 163, 336
EarI CTCTTC 2 cut(s) 163, 336
EciI GGCGGA 1 cut(s) 154
Ecl136II GAGCTC 1 cut(s) 111
EclXI CGGCCG 2 cut(s) 16, 89
Eco130I CCWWGG 1 cut(s) 24
Eco147I AGGCCT 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 113
Eco52I CGGCCG 2 cut(s) 16, 89
Eco53kI GAGCTC 1 cut(s) 111
Eco57I CTGAAG 1 cut(s) 1116
Eco88I CYCGRG 1 cut(s) 112
Eco91I GGTNACC 1 cut(s) 398
EcoICRI GAGCTC 1 cut(s) 111
EcoO65I GGTNACC 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 1067
EcoT14I CCWWGG 1 cut(s) 24
EcoT38I GRGCYC 1 cut(s) 113
EgeI GGCGCC 1 cut(s) 63
EheI GGCGCC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 24
Esp3I CGTCTC 4 cut(s) 247, 273, 293, 410
FaeI CATG 2 cut(s) 28, 124
FaiI YATR 8 cut(s) 26, 122, 369, 428, 834, 974, 1196, 1286
FatI CATG 2 cut(s) 24, 120
FauI CCCGC 1 cut(s) 503
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 6 cut(s) 89, 365, 434, 452, 486, 1282
FokI GGATG 2 cut(s) 262, 821
FriOI GRGCYC 1 cut(s) 113
Fsp4HI GCNGC 6 cut(s) 89, 365, 434, 452, 486, 1282
FspBI CTAG 4 cut(s) 201, 444, 492, 1272
GlaI GCGC 2 cut(s) 63, 306
GluI GCNGC 6 cut(s) 89, 365, 434, 452, 486, 1282
HaeII RGCGCY 1 cut(s) 65
HaeIII GGCC 4 cut(s) 18, 91, 582, 620
HapII CCGG 4 cut(s) 59, 65, 168, 740
HgaI GACGC 1 cut(s) 663
HhaI GCGC 2 cut(s) 64, 307
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 2 cut(s) 28, 124
Hin6I GCGC 2 cut(s) 62, 305
HinP1I GCGC 2 cut(s) 62, 305
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HindIII AAGCTT 2 cut(s) 789, 1098
HinfI GANTC 9 cut(s) 12, 117, 223, 286, 317, 760, 953, 1022, 1133
HpaII CCGG 4 cut(s) 59, 65, 168, 740
HphI GGTGA 4 cut(s) 203, 392, 551, 633
Hpy166II GTNNAC 3 cut(s) 343, 470, 1410
Hpy188III TCNNGA 7 cut(s) 114, 407, 444, 651, 686, 1058, 1130
Hpy8I GTNNAC 3 cut(s) 343, 470, 1410
Hpy99I CGWCG 4 cut(s) 151, 347, 424, 679
HpyAV CCTTC 4 cut(s) 87, 303, 1044, 1193
HpyCH4III ACNGT 5 cut(s) 398, 404, 441, 539, 839
HpyCH4V TGCA 9 cut(s) 271, 814, 847, 860, 866, 1210, 1311, 1319, 1410
HpyF10VI GCNNNNNNNGC 2 cut(s) 53, 485
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 2 cut(s) 28, 124
HspAI GCGC 2 cut(s) 62, 305
KasI GGCGCC 1 cut(s) 61
Kzo9I GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
LmnI GCTCC 4 cut(s) 61, 751, 834, 1042
LpnPI CCDG 8 cut(s) 57, 72, 78, 181, 597, 671, 753, 1397
Lsp1109I GCAGC 2 cut(s) 376, 472
LweI GCATC 2 cut(s) 661, 1304
MaeI CTAG 4 cut(s) 201, 444, 492, 1272
MaeIII GTNAC 4 cut(s) 398, 839, 1006, 1023
MalI GATC 9 cut(s) 22, 174, 232, 376, 448, 505, 515, 588, 655
MboI GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
MfeI CAATTG 2 cut(s) 182, 260
MflI RGATCY 3 cut(s) 172, 513, 586
MhlI GDGCHC 2 cut(s) 113, 1412
MluCI AATT 7 cut(s) 182, 193, 260, 600, 751, 1067, 1265
Mly113I GGCGCC 1 cut(s) 62
MlyI GAGTC 2 cut(s) 311, 1031
MmeI TCCRAC 4 cut(s) 600, 921, 1006, 1242
MseI TTAA 5 cut(s) 51, 624, 711, 999, 1326
MspA1I CMGCKG 1 cut(s) 485
MspCI CTTAAG 1 cut(s) 1325
MspI CCGG 4 cut(s) 59, 65, 168, 740
MunI CAATTG 2 cut(s) 182, 260
Mva1269I GAATGC 3 cut(s) 814, 1114, 1358
MvnI CGCG 2 cut(s) 307, 451
MwoI GCNNNNNNNGC 2 cut(s) 53, 485
NarI GGCGCC 1 cut(s) 62
NcoI CCATGG 1 cut(s) 24
NdeII GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
NlaIII CATG 2 cut(s) 28, 124
NlaIV GGNNCC 3 cut(s) 63, 69, 747
NmeAIII GCCGAG 1 cut(s) 117
NmuCI GTSAC 2 cut(s) 398, 1023
NspV TTCGAA 1 cut(s) 755
PaeR7I CTCGAG 1 cut(s) 112
PaqCI CACCTGC 1 cut(s) 52
PceI AGGCCT 1 cut(s) 620
PcsI WCGNNNNNNNCGW 2 cut(s) 89, 146
PctI GAATGC 3 cut(s) 814, 1114, 1358
PfeI GAWTC 7 cut(s) 12, 117, 223, 286, 760, 953, 1133
PkrI GCNGC 6 cut(s) 90, 366, 435, 453, 487, 1283
PleI GAGTC 2 cut(s) 311, 1030
PluTI GGCGCC 1 cut(s) 65
PpsI GAGTC 2 cut(s) 311, 1030
PshBI ATTAAT 1 cut(s) 51
Psp124BI GAGCTC 1 cut(s) 113
PspEI GGTNACC 1 cut(s) 398
PspN4I GGNNCC 3 cut(s) 63, 69, 747
PspPI GGNCC 1 cut(s) 581
PsuI RGATCY 3 cut(s) 172, 513, 586
RsaI GTAC 1 cut(s) 960
RsaNI GTAC 1 cut(s) 959
SacI GAGCTC 1 cut(s) 113
SalI GTCGAC 1 cut(s) 341
SaqAI TTAA 5 cut(s) 51, 624, 711, 999, 1326
SatI GCNGC 6 cut(s) 89, 365, 434, 452, 486, 1282
Sau3AI GATC 9 cut(s) 20, 172, 230, 374, 446, 503, 513, 586, 653
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 2 cut(s) 311, 1031
SduI GDGCHC 2 cut(s) 113, 1412
SfaNI GCATC 2 cut(s) 661, 1304
SfcI CTRYAG 1 cut(s) 531
SfoI GGCGCC 1 cut(s) 63
Sfr274I CTCGAG 1 cut(s) 112
SfuI TTCGAA 1 cut(s) 755
SlaI CTCGAG 1 cut(s) 112
SmlI CTYRAG 2 cut(s) 112, 1325
SmoI CTYRAG 2 cut(s) 112, 1325
Sse9I AATT 7 cut(s) 182, 193, 260, 600, 751, 1067, 1265
SseBI AGGCCT 1 cut(s) 620
SspDI GGCGCC 1 cut(s) 61
SspMI CTAG 4 cut(s) 201, 444, 492, 1272
SstI GAGCTC 1 cut(s) 113
StuI AGGCCT 1 cut(s) 620
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 5 cut(s) 398, 404, 441, 539, 839
TasI AATT 7 cut(s) 182, 193, 260, 600, 751, 1067, 1265
TatI WGTACW 1 cut(s) 958
TauI GCSGC 4 cut(s) 91, 436, 454, 1284
TfiI GAWTC 7 cut(s) 12, 117, 223, 286, 760, 953, 1133
Tru1I TTAA 5 cut(s) 51, 624, 711, 999, 1326
Tru9I TTAA 5 cut(s) 51, 624, 711, 999, 1326
TscAI CASTG 2 cut(s) 481, 1326
TseFI GTSAC 2 cut(s) 398, 1023
TseI GCWGC 2 cut(s) 364, 485
Tsp45I GTSAC 2 cut(s) 398, 1023
TspDTI ATGAA 5 cut(s) 109, 690, 1080, 1104, 1326
TspGWI ACGGA 2 cut(s) 360, 1025
TspRI CASTG 2 cut(s) 481, 1326
Vha464I CTTAAG 1 cut(s) 1325
VneI GTGCAC 1 cut(s) 1408
VspI ATTAAT 1 cut(s) 51
XapI RAATTY 2 cut(s) 193, 1067
XbaI TCTAGA 1 cut(s) 443
XhoI CTCGAG 1 cut(s) 112
XmiI GTMKAC 1 cut(s) 342
XspI CTAG 4 cut(s) 201, 444, 492, 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.