MD10G1342500.v1.1

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005101
Physical Location & Seq
Reverse (-)
41738790 .. 41742255
3466 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1342500.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGGATCAATTGACCGTAGAGGACGCAGAAGTTTGCGCTCCTCCCCATCCTCCTCCTCCTCCACCACCGACTGCTCAAAGGCGGCCAAGAGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGCGGCTTTCACCGGCGATCTCCCTCATCCCTTGCCTTCCAAATCGCCACTTCCTAAACATCAACACACCATCTTAACTCCATCCTCGACCCTGGCGGAAATTCAGTCGAGGCAGCGGTCTTCGTCTGTGAATAGGCGGCAGAGGCACTTGGACTTGGAGCCCTTGCGCTCCTCCGACGAGAACAGGCCCACCGGATCCTCCTCCATTCACACTCAGAGTCAGATCTGGGGAACCCCTCTCCCACCGGACCAGATGCTTAATTTTAGGAAACAGCGTCCTGCCAAGCCTTTGAAAGAGAACGGAGGAGGAGGAGGAGGCAAAACCCAGCAGCAGCAGAAGCAGCATCTGCCAAAAACCGCACCCTCAAGACCTGACACACCAGTGGCGAGTGCTAGTCTAGATAGGATGTCGAGAGTCAAACTCACTCAGCAGCGGTCTACCAACATAACTGCCACAGCTGCTGCTAAGCTCTTGCAGGCCAGCGGGATGTCTTTACCCGCTCCGCCTGCCACTCCAGTTACCGAGAGTGACACCACTTGCACGGATGTCAATAATCATAGTCAAACGCCAAGCTGTACCGTACGGTGTCTTCCTGACATTCGTTCTTCCATGCCTGAGGCCGATTTGTTGCCAACCGTTTCTAGCAGGCAGCTCTCTGAGAAAAGTAGCAGTAGGGGTAATGCTACTGTCGTTGCCAGTGGCGATTTTTCAAAGTATTCTGCTTCCCCTTGCTCCCGTTCTCTGAATTTGTCATTATCAAGTTCCGACCGCTTATTCTTCCTGACAGACAAAGGCAGTGAAAGAGGGAAGGTGGGTGGCCTATGCCTTCCCCCAGTCCCACCATGTGCTACTGCAAAGCTGGGCCCAGATATAAGGAAAGGAAAGAAAGTTTCCAGTCATCTAGAAGATGTACATTCCTTGAGAGTGCTCCACAACCGCTATCTGCAGTGGAGATATGCCAATGCAAGAGCAGAGGCTTCCGTGCGAGCTCAGCAGAGAGAGACCCAGAGAACACTTTATTCTCTCGCGGTAAAGATTGCTGAACTGTATGATTCTGTGAAGAGGAAACGGATAGAACTTGGCATTTTGCAAAGAACAAAGACTTTATCCACAATTCTTGAAGCTCAAATTCCATATTTGGACCAGTGGTTTTCTCTTGAAGGGGATTATTCAGTTTCTCTGGCAGAAGCAACTCAAGCATTGTTGAATGCCTCAGTTCAACTTCCAAGCAGTGGCAGTGTTAGGGCCAATCTACGAGAGTTAGAAGAGGCACTGAGCTCAGCAATAGAAGTGATGGACATAATAGTTTTCCATGTTCACCGATCTTTGCCAAAGGCAGAAGATACAGAACATTTGATTTCGGAACTAGCCAGAGTGATTGGTGGAGAAAGAGCTCTCATTGAAGAATGCGGAAATCTGCTGTCTAAGACCTATACAACGCAGGTGGAAGAGTGGAGCTTAAGAAGCCAGATAATCCAATCGAAATGTTGCTGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

541

Amino Acids

59.21

Weight (kDa)

9.31

Isoelectric Point (pI)

66.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 236 - 509 8.9e-52 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1555
Acc36I ACCTGC 1 cut(s) 1555
AccB7I CCANNNNNTGG 1 cut(s) 1355
AccBSI CCGCTC 1 cut(s) 623
AccI GTMKAC 1 cut(s) 560
AccII CGCG 1 cut(s) 1151
AclWI GGATC 3 cut(s) 12, 312, 325
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 3 cut(s) 222, 868, 1251
AdeI CACNNNGTG 1 cut(s) 968
AfaI GTAC 3 cut(s) 700, 705, 1035
AfiI CCNNNNNNNGG 1 cut(s) 1355
AflII CTTAAG 1 cut(s) 1582
AgsI TTSAA 7 cut(s) 415, 834, 1244, 1283, 1330, 1343, 1526
AjnI CCWGG 1 cut(s) 213
AleI CACNNNNGTG 1 cut(s) 503
Alw21I GWGCWC 5 cut(s) 107, 1053, 1114, 1403, 1519
Alw26I GTCTC 2 cut(s) 123, 1118
AlwI GGATC 3 cut(s) 12, 312, 325
AlwNI CAGNNNCTG 2 cut(s) 469, 584
AoxI GGCC 7 cut(s) 83, 308, 600, 741, 940, 985, 1368
ApaI GGGCCC 1 cut(s) 989
ApeKI GCWGC 8 cut(s) 235, 451, 454, 463, 553, 581, 584, 772
ApoI RAATTY 3 cut(s) 222, 868, 1251
AspLEI GCGC 2 cut(s) 38, 291
AspS9I GGNCC 6 cut(s) 309, 370, 985, 986, 1264, 1368
AsuHPI GGTGA 3 cut(s) 112, 124, 1433
AvaII GGWCC 2 cut(s) 370, 1264
AxyI CCTNAGG 1 cut(s) 738
BaeGI GKGCMC 1 cut(s) 989
BamHI GGATCC 1 cut(s) 317
BanII GRGCYC 6 cut(s) 107, 285, 989, 1114, 1403, 1519
BarI GAAGNNNNNNTAC 2 cut(s) 696, 728
BbsI GAAGAC 2 cut(s) 234, 704
Bbv12I GWGCWC 5 cut(s) 107, 1053, 1114, 1403, 1519
BbvI GCAGC 8 cut(s) 247, 463, 466, 475, 565, 568, 571, 784
BccI CCATC 4 cut(s) 54, 200, 211, 1411
BciT130I CCWGG 1 cut(s) 215
BcoDI GTCTC 2 cut(s) 123, 1118
BfaI CTAG 6 cut(s) 108, 516, 521, 765, 1025, 1490
BfmI CTRYAG 1 cut(s) 1067
BfrI CTTAAG 1 cut(s) 1582
BfuAI ACCTGC 1 cut(s) 1555
BglII AGATCT 1 cut(s) 345
BlpI GCTNAGC 3 cut(s) 588, 1113, 1402
Bme1390I CCNGG 1 cut(s) 215
Bme18I GGWCC 2 cut(s) 370, 1264
BmgT120I GGNCC 6 cut(s) 309, 370, 985, 986, 1264, 1368
BmiI GGNNCC 4 cut(s) 282, 319, 355, 987
BmrFI CCNGG 1 cut(s) 215
BmrI ACTGGG 1 cut(s) 950
BmsI GCATC 2 cut(s) 366, 475
BmuI ACTGGG 1 cut(s) 950
BpiI GAAGAC 2 cut(s) 234, 704
BplI GAGNNNNNCTC 4 cut(s) 89, 121, 528, 560
BpmI CTGGAG 1 cut(s) 621
Bpu1102I GCTNAGC 3 cut(s) 588, 1113, 1402
BpuEI CTTGAG 3 cut(s) 472, 1063, 1302
BsaI GGTCTC 1 cut(s) 1118
BsaJI CCNNGG 1 cut(s) 213
BsaWI WCCGGW 2 cut(s) 314, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 1355
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 6 cut(s) 503, 638, 819, 956, 1017, 1267
Bse21I CCTNAGG 1 cut(s) 738
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 1 cut(s) 213
BseGI GGATG 6 cut(s) 46, 148, 203, 534, 615, 673
BseLI CCNNNNNNNGG 1 cut(s) 1355
BseMII CTCAG 8 cut(s) 350, 563, 729, 771, 1127, 1350, 1388, 1416
BseNI ACTGG 6 cut(s) 503, 638, 819, 956, 1017, 1267
BseSI GKGCMC 1 cut(s) 989
BseXI GCAGC 8 cut(s) 247, 463, 466, 475, 565, 568, 571, 784
BseYI CCCAGC 2 cut(s) 447, 982
Bsh1236I CGCG 1 cut(s) 1151
Bsh1285I CGRYCG 1 cut(s) 892
BshFI GGCC 7 cut(s) 85, 310, 602, 743, 942, 987, 1370
BsiEI CGRYCG 1 cut(s) 892
BsiHKAI GWGCWC 5 cut(s) 107, 1053, 1114, 1403, 1519
BsiSI CCGG 3 cut(s) 135, 315, 368
BsiWI CGTACG 1 cut(s) 703
BslFI GGGAC 1 cut(s) 944
BslI CCNNNNNNNGG 1 cut(s) 1355
BsmAI GTCTC 2 cut(s) 123, 1118
BsmFI GGGAC 1 cut(s) 944
BsmI GAATGC 2 cut(s) 1336, 1535
BsnI GGCC 7 cut(s) 85, 310, 602, 743, 942, 987, 1370
Bso31I GGTCTC 1 cut(s) 1118
Bsp120I GGGCCC 1 cut(s) 985
Bsp1286I GDGCHC 7 cut(s) 107, 285, 989, 1053, 1114, 1403, 1519
Bsp1407I TGTACA 1 cut(s) 1033
Bsp143I GATC 5 cut(s) 4, 139, 317, 345, 1445
Bsp1720I GCTNAGC 3 cut(s) 588, 1113, 1402
BspANI GGCC 7 cut(s) 85, 310, 602, 743, 942, 987, 1370
BspCNI CTCAG 8 cut(s) 349, 562, 730, 772, 1126, 1349, 1389, 1415
BspFNI CGCG 1 cut(s) 1151
BspLI GGNNCC 4 cut(s) 282, 319, 355, 987
BspMAI CTGCAG 1 cut(s) 1071
BspMI ACCTGC 1 cut(s) 1555
BspPI GGATC 3 cut(s) 12, 312, 325
BspTI CTTAAG 1 cut(s) 1582
BspTNI GGTCTC 1 cut(s) 1118
BsrBI CCGCTC 1 cut(s) 623
BsrFI RCCGGY 1 cut(s) 134
BsrGI TGTACA 1 cut(s) 1033
BsrI ACTGG 6 cut(s) 503, 638, 819, 956, 1017, 1267
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 213
BssMI GATC 5 cut(s) 4, 139, 317, 345, 1445
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 6 cut(s) 16, 703, 708, 760, 811, 1170
Bst6I CTCTTC 3 cut(s) 1178, 1383, 1566
BstAFI CTTAAG 1 cut(s) 1582
BstAPI GCANNNNNTGC 1 cut(s) 469
BstAUI TGTACA 1 cut(s) 1033
BstC8I GCNNGC 5 cut(s) 600, 604, 630, 770, 1110
BstF5I GGATG 6 cut(s) 46, 148, 203, 534, 615, 673
BstFNI CGCG 1 cut(s) 1151
BstHHI GCGC 2 cut(s) 38, 291
BstKTI GATC 5 cut(s) 7, 142, 320, 348, 1448
BstMAI GTCTC 2 cut(s) 123, 1118
BstMBI GATC 5 cut(s) 4, 139, 317, 345, 1445
BstMCI CGRYCG 1 cut(s) 892
BstMWI GCNNNNNNNGC 9 cut(s) 265, 460, 463, 469, 581, 629, 1114, 1319, 1587
BstNI CCWGG 1 cut(s) 215
BstSCI CCNGG 1 cut(s) 213
BstSFI CTRYAG 1 cut(s) 1067
BstSLI GKGCMC 1 cut(s) 989
BstUI CGCG 1 cut(s) 1151
BstV1I GCAGC 8 cut(s) 247, 463, 466, 475, 565, 568, 571, 784
BstV2I GAAGAC 2 cut(s) 234, 704
BstX2I RGATCY 2 cut(s) 317, 345
BstYI RGATCY 2 cut(s) 317, 345
Bsu36I CCTNAGG 1 cut(s) 738
BsuRI GGCC 7 cut(s) 85, 310, 602, 743, 942, 987, 1370
BtsCI GGATG 6 cut(s) 46, 148, 203, 534, 615, 673
BtsI GCAGTG 4 cut(s) 925, 1076, 1360, 1366
BtsIMutI CAGTG 8 cut(s) 510, 826, 925, 1076, 1274, 1360, 1366, 1394
BveI ACCTGC 1 cut(s) 1555
Cac8I GCNNGC 5 cut(s) 600, 604, 630, 770, 1110
CaiI CAGNNNCTG 2 cut(s) 469, 584
CfoI GCGC 2 cut(s) 38, 291
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 6 cut(s) 309, 370, 985, 986, 1264, 1368
CseI GACGC 2 cut(s) 32, 386
Csp6I GTAC 3 cut(s) 699, 704, 1034
CspCI CAANNNNNGTGG 4 cut(s) 1222, 1257, 1548, 1583
CviAII CATG 4 cut(s) 115, 733, 966, 1436
CviQI GTAC 3 cut(s) 699, 704, 1034
DpnI GATC 5 cut(s) 6, 141, 319, 347, 1447
DpnII GATC 5 cut(s) 4, 139, 317, 345, 1445
DraIII CACNNNGTG 1 cut(s) 968
EaeI YGGCCR 1 cut(s) 83
Eam1104I CTCTTC 3 cut(s) 1178, 1383, 1566
EarI CTCTTC 3 cut(s) 1178, 1383, 1566
EciI GGCGGA 2 cut(s) 233, 615
Ecl136II GAGCTC 4 cut(s) 105, 1112, 1401, 1517
Eco24I GRGCYC 6 cut(s) 107, 285, 989, 1114, 1403, 1519
Eco31I GGTCTC 1 cut(s) 1118
Eco47I GGWCC 2 cut(s) 370, 1264
Eco53kI GAGCTC 4 cut(s) 105, 1112, 1401, 1517
Eco81I CCTNAGG 1 cut(s) 738
EcoICRI GAGCTC 4 cut(s) 105, 1112, 1401, 1517
EcoRII CCWGG 1 cut(s) 213
EcoT38I GRGCYC 6 cut(s) 107, 285, 989, 1114, 1403, 1519
FaeI CATG 4 cut(s) 118, 736, 969, 1439
FaqI GGGAC 1 cut(s) 944
FatI CATG 4 cut(s) 114, 732, 965, 1435
FauI CCCGC 2 cut(s) 599, 628
FblI GTMKAC 1 cut(s) 560
FokI GGATG 6 cut(s) 33, 135, 190, 541, 622, 680
FriOI GRGCYC 6 cut(s) 107, 285, 989, 1114, 1403, 1519
FspBI CTAG 6 cut(s) 108, 516, 521, 765, 1025, 1490
GlaI GCGC 2 cut(s) 37, 290
GsaI CCCAGC 2 cut(s) 451, 986
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 7 cut(s) 85, 310, 602, 743, 942, 987, 1370
HapII CCGG 3 cut(s) 135, 315, 368
HgaI GACGC 2 cut(s) 32, 386
HhaI GCGC 2 cut(s) 38, 291
Hin1II CATG 4 cut(s) 118, 736, 969, 1439
Hin6I GCGC 2 cut(s) 36, 289
HinP1I GCGC 2 cut(s) 36, 289
HinfI GANTC 3 cut(s) 340, 537, 1175
HpaII CCGG 3 cut(s) 135, 315, 368
HphI GGTGA 3 cut(s) 112, 124, 1433
Hpy166II GTNNAC 2 cut(s) 561, 1441
Hpy188I TCNGA 7 cut(s) 298, 339, 345, 781, 867, 889, 1486
Hpy188III TCNNGA 9 cut(s) 489, 521, 534, 716, 904, 1025, 1241, 1280, 1620
Hpy8I GTNNAC 2 cut(s) 561, 1441
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 4 cut(s) 168, 925, 959, 1277
HpyCH4III ACNGT 6 cut(s) 16, 703, 708, 760, 811, 1170
HpyCH4V TGCA 6 cut(s) 598, 663, 977, 1069, 1088, 1213
HpyF10VI GCNNNNNNNGC 9 cut(s) 265, 460, 463, 469, 581, 629, 1114, 1319, 1587
Hsp92II CATG 4 cut(s) 118, 736, 969, 1439
HspAI GCGC 2 cut(s) 36, 289
Kzo9I GATC 5 cut(s) 4, 139, 317, 345, 1445
LmnI GCTCC 7 cut(s) 43, 280, 296, 628, 860, 1056, 1578
Lsp1109I GCAGC 8 cut(s) 247, 463, 466, 475, 565, 568, 571, 784
LweI GCATC 2 cut(s) 366, 475
MaeI CTAG 6 cut(s) 108, 516, 521, 765, 1025, 1490
MaeIII GTNAC 2 cut(s) 640, 650
MalI GATC 5 cut(s) 6, 141, 319, 347, 1447
MbiI CCGCTC 1 cut(s) 623
MboI GATC 5 cut(s) 4, 139, 317, 345, 1445
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 2 cut(s) 317, 345
MhlI GDGCHC 7 cut(s) 107, 285, 989, 1053, 1114, 1403, 1519
MluCI AATT 6 cut(s) 8, 222, 382, 868, 1236, 1251
MlyI GAGTC 2 cut(s) 349, 546
MmeI TCCRAC 2 cut(s) 321, 912
MseI TTAA 3 cut(s) 197, 381, 1583
MslI CAYNNNNRTG 1 cut(s) 503
MspA1I CMGCKG 4 cut(s) 238, 556, 581, 606
MspCI CTTAAG 1 cut(s) 1582
MspI CCGG 3 cut(s) 135, 315, 368
MspR9I CCNGG 1 cut(s) 215
MunI CAATTG 1 cut(s) 8
Mva1269I GAATGC 2 cut(s) 1336, 1535
MvaI CCWGG 1 cut(s) 215
MvnI CGCG 1 cut(s) 1151
MwoI GCNNNNNNNGC 9 cut(s) 265, 460, 463, 469, 581, 629, 1114, 1319, 1587
NdeII GATC 5 cut(s) 4, 139, 317, 345, 1445
NlaIII CATG 4 cut(s) 118, 736, 969, 1439
NlaIV GGNNCC 4 cut(s) 282, 319, 355, 987
NmuCI GTSAC 1 cut(s) 650
OliI CACNNNNGTG 1 cut(s) 503
PaqCI CACCTGC 1 cut(s) 1555
PctI GAATGC 2 cut(s) 1336, 1535
PfeI GAWTC 1 cut(s) 1175
Pfl23II CGTACG 1 cut(s) 703
PflMI CCANNNNNTGG 1 cut(s) 1355
PleI GAGTC 2 cut(s) 348, 545
PpsI GAGTC 2 cut(s) 348, 545
Psp124BI GAGCTC 4 cut(s) 107, 1114, 1403, 1519
Psp6I CCWGG 1 cut(s) 213
PspFI CCCAGC 2 cut(s) 447, 982
PspGI CCWGG 1 cut(s) 213
PspLI CGTACG 1 cut(s) 703
PspN4I GGNNCC 4 cut(s) 282, 319, 355, 987
PspOMI GGGCCC 1 cut(s) 985
PspPI GGNCC 6 cut(s) 309, 370, 985, 986, 1264, 1368
PstI CTGCAG 1 cut(s) 1071
PstNI CAGNNNCTG 2 cut(s) 469, 584
PsuI RGATCY 2 cut(s) 317, 345
PvuII CAGCTG 1 cut(s) 581
RsaI GTAC 3 cut(s) 700, 705, 1035
RsaNI GTAC 3 cut(s) 699, 704, 1034
RseI CAYNNNNRTG 1 cut(s) 503
SacI GAGCTC 4 cut(s) 107, 1114, 1403, 1519
SaqAI TTAA 3 cut(s) 197, 381, 1583
Sau3AI GATC 5 cut(s) 4, 139, 317, 345, 1445
Sau96I GGNCC 6 cut(s) 309, 370, 985, 986, 1264, 1368
SchI GAGTC 2 cut(s) 349, 546
ScrFI CCNGG 1 cut(s) 215
SduI GDGCHC 7 cut(s) 107, 285, 989, 1053, 1114, 1403, 1519
SfaNI GCATC 2 cut(s) 366, 475
SfcI CTRYAG 1 cut(s) 1067
SgrAI CRCCGGYG 1 cut(s) 134
SinI GGWCC 2 cut(s) 370, 1264
SmiMI CAYNNNNRTG 1 cut(s) 503
SmlI CTYRAG 4 cut(s) 487, 1042, 1317, 1582
SmoI CTYRAG 4 cut(s) 487, 1042, 1317, 1582
Sse9I AATT 6 cut(s) 8, 222, 382, 868, 1236, 1251
SspMI CTAG 6 cut(s) 108, 516, 521, 765, 1025, 1490
SstI GAGCTC 4 cut(s) 107, 1114, 1403, 1519
StyD4I CCNGG 1 cut(s) 213
TaaI ACNGT 6 cut(s) 16, 703, 708, 760, 811, 1170
TaqI TCGA 4 cut(s) 209, 230, 533, 1604
TasI AATT 6 cut(s) 8, 222, 382, 868, 1236, 1251
TatI WGTACW 1 cut(s) 1033
TauI GCSGC 3 cut(s) 85, 128, 262
TfiI GAWTC 1 cut(s) 1175
Tru1I TTAA 3 cut(s) 197, 381, 1583
Tru9I TTAA 3 cut(s) 197, 381, 1583
TscAI CASTG 8 cut(s) 510, 826, 925, 1076, 1274, 1360, 1366, 1401
TseFI GTSAC 1 cut(s) 650
TseI GCWGC 8 cut(s) 235, 451, 454, 463, 553, 581, 584, 772
Tsp45I GTSAC 1 cut(s) 650
TspDTI ATGAA 1 cut(s) 103
TspGWI ACGGA 4 cut(s) 438, 680, 1093, 1207
TspRI CASTG 8 cut(s) 510, 826, 925, 1076, 1274, 1360, 1366, 1401
Van91I CCANNNNNTGG 1 cut(s) 1355
Vha464I CTTAAG 1 cut(s) 1582
VpaK11BI GGWCC 2 cut(s) 370, 1264
XapI RAATTY 3 cut(s) 222, 868, 1251
XbaI TCTAGA 2 cut(s) 520, 1024
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 6 cut(s) 108, 516, 521, 765, 1025, 1490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.