MD00G1208500.v1.1

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005101
Physical Location & Seq
Reverse (-)
50799687 .. 50800213
527 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1208500.v1.1.491

Sequence Viewer

Length: 432 bp
ATGGATCAATCGACCGTGGAAAATTGCGCATCTGCCACCGTAGAGGACGCAGAAGCTTGCGCTCCTCCCCATCCTCTTCCTCCTCCGCCGCCGACTGCTCAAAGGCGGCCAAGAGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGCGGCTTCCACCGGTGATCTCCCTCATCCCTTGCCTGCCAAATCGCCCCTTCCTAAATATCAACAAACCATCTTAACTCCATCCTCGACCCTGGCCGAAATTCAGTCGAGGCAGCGGTCTTCCTCTGTGAATAGGCGGCGGAGGCACTTGGACTTGGAGCCCTTGCGCTGCTCCGACGAGAACAGGCCCACCGGCCCCTCCTCCATTCACACTCAGAATCAGATCTGGGAAACCCCTCTCCCACCGGACCAGATGCTTAATTATAGAAAACAGCGTCCCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

144

Amino Acids

15.96

Weight (kDa)

9.35

Isoelectric Point (pI)

91.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 28
AciI CCGC 8 cut(s) 86, 89, 106, 149, 262, 283, 286, 426
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 2 cut(s) 107, 240
AcsI RAATTY 1 cut(s) 246
AgeI ACCGGT 1 cut(s) 158
AjnI CCWGG 1 cut(s) 237
AluBI AGCT 2 cut(s) 56, 129
AluI AGCT 2 cut(s) 56, 129
Alw21I GWGCWC 1 cut(s) 131
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 1 cut(s) 12
AoxI GGCC 4 cut(s) 107, 240, 332, 340
ApeKI GCWGC 2 cut(s) 259, 315
ApoI RAATTY 1 cut(s) 246
AsiGI ACCGGT 1 cut(s) 158
AspLEI GCGC 3 cut(s) 29, 62, 315
AspS9I GGNCC 3 cut(s) 333, 341, 394
AsuHPI GGTGA 2 cut(s) 136, 173
AvaII GGWCC 1 cut(s) 394
BanII GRGCYC 2 cut(s) 131, 309
BbsI GAAGAC 1 cut(s) 258
Bbv12I GWGCWC 1 cut(s) 131
BbvI GCAGC 2 cut(s) 271, 302
BccI CCATC 3 cut(s) 78, 224, 235
BciT130I CCWGG 1 cut(s) 239
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 2 cut(s) 132, 430
BglII AGATCT 1 cut(s) 369
BisI GCNGC 6 cut(s) 89, 107, 150, 260, 284, 316
BlsI GCNGC 6 cut(s) 90, 108, 151, 261, 285, 317
Bme1390I CCNGG 1 cut(s) 239
Bme18I GGWCC 1 cut(s) 394
BmgT120I GGNCC 3 cut(s) 333, 341, 394
BmiI GGNNCC 2 cut(s) 306, 343
BmrFI CCNGG 1 cut(s) 239
BmsI GCATC 2 cut(s) 38, 390
BpiI GAAGAC 1 cut(s) 258
BplI GAGNNNNNCTC 2 cut(s) 113, 145
BsaJI CCNNGG 2 cut(s) 15, 237
BsaWI WCCGGW 2 cut(s) 158, 391
Bse118I RCCGGY 2 cut(s) 158, 338
BseBI CCWGG 1 cut(s) 239
BseDI CCNNGG 2 cut(s) 15, 237
BseGI GGATG 3 cut(s) 70, 172, 227
BseMII CTCAG 1 cut(s) 374
BseRI GAGGAG 3 cut(s) 54, 72, 337
BseXI GCAGC 2 cut(s) 271, 302
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 4 cut(s) 109, 242, 334, 342
BshTI ACCGGT 1 cut(s) 158
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 131
BsiSI CCGG 3 cut(s) 159, 339, 392
BslFI GGGAC 1 cut(s) 408
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 408
BsnI GGCC 4 cut(s) 109, 242, 334, 342
Bsp1286I GDGCHC 2 cut(s) 131, 309
Bsp143I GATC 3 cut(s) 4, 163, 369
BspACI CCGC 8 cut(s) 86, 89, 106, 149, 262, 283, 286, 426
BspANI GGCC 4 cut(s) 109, 242, 334, 342
BspCNI CTCAG 1 cut(s) 373
BspLI GGNNCC 2 cut(s) 306, 343
BspPI GGATC 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 158, 338
BssAI RCCGGY 2 cut(s) 158, 338
BssECI CCNNGG 2 cut(s) 15, 237
BssMI GATC 3 cut(s) 4, 163, 369
Bst2UI CCWGG 1 cut(s) 239
Bst4CI ACNGT 2 cut(s) 16, 40
Bst6I CTCTTC 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 58, 183
BstDEI CTNAG 1 cut(s) 360
BstDSI CCRYGG 1 cut(s) 15
BstF5I GGATG 3 cut(s) 70, 172, 227
BstHHI GCGC 3 cut(s) 29, 62, 315
BstKTI GATC 3 cut(s) 7, 166, 372
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 3 cut(s) 4, 163, 369
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 1 cut(s) 289
BstNI CCWGG 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 237
BstV1I GCAGC 2 cut(s) 271, 302
BstV2I GAAGAC 1 cut(s) 258
BstX2I RGATCY 1 cut(s) 369
BstYI RGATCY 1 cut(s) 369
BsuRI GGCC 4 cut(s) 109, 242, 334, 342
BtgI CCRYGG 1 cut(s) 15
BtsCI GGATG 3 cut(s) 70, 172, 227
Cac8I GCNNGC 2 cut(s) 58, 183
CfoI GCGC 3 cut(s) 29, 62, 315
Cfr10I RCCGGY 2 cut(s) 158, 338
Cfr13I GGNCC 3 cut(s) 333, 341, 394
CseI GACGC 2 cut(s) 56, 410
CspAI ACCGGT 1 cut(s) 158
CviAII CATG 1 cut(s) 139
CviJI RGCY 8 cut(s) 56, 109, 129, 152, 242, 307, 334, 342
CviKI_1 RGCY 8 cut(s) 56, 109, 129, 152, 242, 307, 334, 342
DdeI CTNAG 1 cut(s) 360
DpnI GATC 3 cut(s) 6, 165, 371
DpnII GATC 3 cut(s) 4, 163, 369
EaeI YGGCCR 2 cut(s) 107, 240
Eam1104I CTCTTC 1 cut(s) 81
EarI CTCTTC 1 cut(s) 81
EciI GGCGGA 2 cut(s) 75, 301
Ecl136II GAGCTC 1 cut(s) 129
Eco24I GRGCYC 2 cut(s) 131, 309
Eco47I GGWCC 1 cut(s) 394
Eco53kI GAGCTC 1 cut(s) 129
EcoICRI GAGCTC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 237
EcoT38I GRGCYC 2 cut(s) 131, 309
FaeI CATG 1 cut(s) 142
FaiI YATR 2 cut(s) 140, 411
FaqI GGGAC 1 cut(s) 408
FatI CATG 1 cut(s) 138
Fnu4HI GCNGC 6 cut(s) 89, 107, 150, 260, 284, 316
FokI GGATG 3 cut(s) 57, 159, 214
FriOI GRGCYC 2 cut(s) 131, 309
Fsp4HI GCNGC 6 cut(s) 89, 107, 150, 260, 284, 316
FspBI CTAG 2 cut(s) 132, 430
FspI TGCGCA 1 cut(s) 28
GlaI GCGC 3 cut(s) 28, 61, 314
GluI GCNGC 6 cut(s) 89, 107, 150, 260, 284, 316
HaeIII GGCC 4 cut(s) 109, 242, 334, 342
HapII CCGG 3 cut(s) 159, 339, 392
HgaI GACGC 2 cut(s) 56, 410
HhaI GCGC 3 cut(s) 29, 62, 315
Hin1II CATG 1 cut(s) 142
Hin6I GCGC 3 cut(s) 27, 60, 313
HinP1I GCGC 3 cut(s) 27, 60, 313
HindIII AAGCTT 1 cut(s) 54
HinfI GANTC 1 cut(s) 364
HpaII CCGG 3 cut(s) 159, 339, 392
HphI GGTGA 2 cut(s) 136, 173
Hpy188I TCNGA 3 cut(s) 322, 363, 369
Hpy99I CGWCG 1 cut(s) 326
HpyAV CCTTC 1 cut(s) 206
HpyCH4III ACNGT 2 cut(s) 16, 40
HpyF10VI GCNNNNNNNGC 1 cut(s) 289
HpyF3I CTNAG 1 cut(s) 360
Hsp92II CATG 1 cut(s) 142
HspAI GCGC 3 cut(s) 27, 60, 313
Kzo9I GATC 3 cut(s) 4, 163, 369
LmnI GCTCC 3 cut(s) 67, 304, 323
Lsp1109I GCAGC 2 cut(s) 271, 302
LweI GCATC 2 cut(s) 38, 390
MaeI CTAG 2 cut(s) 132, 430
MalI GATC 3 cut(s) 6, 165, 371
MboI GATC 3 cut(s) 4, 163, 369
MboII GAAGA 2 cut(s) 68, 258
MflI RGATCY 1 cut(s) 369
MhlI GDGCHC 2 cut(s) 131, 309
MluCI AATT 3 cut(s) 22, 246, 406
MmeI TCCRAC 1 cut(s) 345
MseI TTAA 2 cut(s) 221, 405
MspA1I CMGCKG 1 cut(s) 262
MspI CCGG 3 cut(s) 159, 339, 392
MspR9I CCNGG 1 cut(s) 239
MvaI CCWGG 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 289
NdeII GATC 3 cut(s) 4, 163, 369
NlaIII CATG 1 cut(s) 142
NlaIV GGNNCC 2 cut(s) 306, 343
NsbI TGCGCA 1 cut(s) 28
PfeI GAWTC 1 cut(s) 364
PinAI ACCGGT 1 cut(s) 158
PkrI GCNGC 6 cut(s) 90, 108, 151, 261, 285, 317
Psp124BI GAGCTC 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 237
PspGI CCWGG 1 cut(s) 237
PspN4I GGNNCC 2 cut(s) 306, 343
PspPI GGNCC 3 cut(s) 333, 341, 394
PsuI RGATCY 1 cut(s) 369
SacI GAGCTC 1 cut(s) 131
SaqAI TTAA 2 cut(s) 221, 405
SatI GCNGC 6 cut(s) 89, 107, 150, 260, 284, 316
Sau3AI GATC 3 cut(s) 4, 163, 369
Sau96I GGNCC 3 cut(s) 333, 341, 394
ScrFI CCNGG 1 cut(s) 239
SduI GDGCHC 2 cut(s) 131, 309
SetI ASST 4 cut(s) 58, 126, 131, 137
SfaNI GCATC 2 cut(s) 38, 390
SgrAI CRCCGGYG 1 cut(s) 158
SinI GGWCC 1 cut(s) 394
Sse9I AATT 3 cut(s) 22, 246, 406
SsiI CCGC 8 cut(s) 86, 89, 106, 149, 262, 283, 286, 426
SspMI CTAG 2 cut(s) 132, 430
SstI GAGCTC 1 cut(s) 131
StyD4I CCNGG 1 cut(s) 237
TaaI ACNGT 2 cut(s) 16, 40
TaqI TCGA 3 cut(s) 11, 233, 254
TasI AATT 3 cut(s) 22, 246, 406
TauI GCSGC 4 cut(s) 91, 109, 152, 286
TfiI GAWTC 1 cut(s) 364
Tru1I TTAA 2 cut(s) 221, 405
Tru9I TTAA 2 cut(s) 221, 405
TseI GCWGC 2 cut(s) 259, 315
TspDTI ATGAA 1 cut(s) 127
VpaK11BI GGWCC 1 cut(s) 394
XapI RAATTY 1 cut(s) 246
XspI CTAG 2 cut(s) 132, 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.