Rroxscaffold_1G00076040

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005101
Physical Location & Seq
Forward (+)
96591556 .. 96596132
4577 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00076040.1

Sequence Viewer

Length: 1563 bp
ATGGATCAATTGATGGTGGAAAAGTTGGCATCGGGCACGGGTGAGGGCGCAGCAGCCCCTCATCCTCAGCGGCGGCCAAGGGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGTGGCCGGTGATCCCTTTTCTACCAGATCACCTCTTCCTAAACATCAACAGGGACAGGGTGTCTCAACTCCAAGCCCGTACCTTGCAGAAAGACAGAGGTCATCCTCCGTGAATAGGCCACGGAGGCACTTGGACTTGGAGCCATTCCGCTGCTCTGACGAGAACAGGCCCGCCCGCCCCGAGTCTCCTCTGCCGCCAGATCAGGCAAGGAAACAGCGTCCTGTCCCTGTCCCTGTCCCTTTTAAAGAGAACGGAGGAGGAGGAGGCAAACCCCAACAGCATCATCTTCCTAAAACTTGCCCTGGGAAAGGAAGCAATAATGCTTTTACTACACCCTCACGACCTGACACACCCATGGTGACTGCTAGTCTCGATAGGACAAGAATCAGACAGCGGTCTTCCAATTCCAATGTCACTAGTGCCACAGCAGCTACTAAGCTGTTGCAATCTACCACTGAGGCCACCTCCAGTGACACTAATCACACTCAAATCACCAGAAGGTGTCTTCCTGATGTTCGCTCCTCCATGCCCGAGGCCGATATGTTGTTGCCGACTGTTTCCAGTAGACAGACGACCGAAAAAAGTTGTAACAGGCCTAATGCAACTGTCAATGGAAGCGATTTTTCAAAATTTTCTGCTTCCCCTTGCTCCCGTTCTCTCAACTTGCCACTGTCAAGCTCCGAGCACTTACTCTTCCAGTCAATCAAAGGCAGTGACAAATTAGAATCTGCTTTCTCCAAGCCATATGCAAATGCAGTGAAGATGGGCGGCCTCTGTCTGCCCCCAGTCCCACCATGTGCTAGTGCAAAGCCAGGTTCAGAAATAAGGAAAGGAAAGAAAGTTTCTAGTCTCCGAGAAGATGCTCATTCCTTGAGATTGCTTCAAAACCGCTATTTGCAGTGGAGATATGCCAATGCAACAGCACAGGCTTCCATGCTAGCTCAGCAGAGAGAGACTGAGAGAACACATTATTCTCTTGCATTAAAGATAACAGAACTATATGATTCTGTGAAGAGAAAACGGATAGACCTTGGCGTTTTGCAAAGAACAAAGACTTTATCAACAATTCTTGAAGCTCAAATTCCATATTTGGATCAGTGGTTTTCTCTTCAAGAGGATTATTCAATTTCTCTGGCAGAAGCAACTCAAGCTTTGTTGAATGCATCACTCCGGCTTCCAATCAGCACCAATGTTAAGGCTAATAAACAAGAAGTAGAAGAGGCACTGAACTCAGCAATGGAAGTGATGGAGAAAATAGTTTTCCATGTTCAGCAATCTATGCCGAAGGCAGAGGAAACAGACGATTTGATTTCGGATCTAGCAAGAGTGATTGGTGGAGAAAGAGCTTTCGTTGAAGAATGTGGAAATCTGTTGTCCAAGACATATACAAAACAGGTGGAAGAGTGTAGCTTAAGGGGCCATATAATTCAGTTGAAACAACACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

520

Amino Acids

57.46

Weight (kDa)

9.46

Isoelectric Point (pI)

64.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 177 - 489 9.5e-50 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 679
AciI CCGC 9 cut(s) 70, 73, 262, 285, 289, 308, 508, 882, 1003
AclWI GGATC 4 cut(s) 12, 119, 1215, 1437
AcoI YGGCCR 2 cut(s) 74, 117
AcsI RAATTY 2 cut(s) 743, 1194
AdeI CACNNNGTG 1 cut(s) 911
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 119, 228, 422
AflII CTTAAG 1 cut(s) 1525
AgsI TTSAA 8 cut(s) 741, 998, 1187, 1226, 1239, 1273, 1469, 1549
AhdI GACNNNNNGTC 2 cut(s) 173, 480
AhlI ACTAGT 1 cut(s) 530
AjnI CCWGG 2 cut(s) 415, 925
AluBI AGCT 9 cut(s) 96, 545, 553, 792, 1055, 1190, 1265, 1460, 1524
AluI AGCT 9 cut(s) 96, 545, 553, 792, 1055, 1190, 1265, 1460, 1524
Alw21I GWGCWC 2 cut(s) 98, 801
Alw26I GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
AlwI GGATC 4 cut(s) 12, 119, 1215, 1437
Ama87I CYCGRG 2 cut(s) 293, 644
AoxI GGCC 9 cut(s) 74, 117, 230, 281, 573, 648, 707, 883, 1531
ApeKI GCWGC 4 cut(s) 50, 53, 264, 542
ApoI RAATTY 2 cut(s) 743, 1194
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 282, 1531
AsuHPI GGTGA 7 cut(s) 53, 94, 103, 134, 135, 484, 598
AsuNHI GCTAGC 1 cut(s) 1051
AvaI CYCGRG 2 cut(s) 293, 644
BaeGI GKGCMC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 504, 611
Bbv12I GWGCWC 2 cut(s) 98, 801
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 4 cut(s) 62, 65, 251, 554
BccI CCATC 3 cut(s) 7, 871, 1354
BciT130I CCWGG 2 cut(s) 417, 927
BcoDI GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
BcuI ACTAGT 1 cut(s) 530
BfaI CTAG 7 cut(s) 99, 480, 531, 915, 960, 1052, 1433
BfrI CTTAAG 1 cut(s) 1525
BglI GCCNNNNNGGC 1 cut(s) 238
BisI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 308, 543, 883
BlpI GCTNAGC 1 cut(s) 1056
BlsI GCNGC 8 cut(s) 52, 55, 72, 75, 266, 309, 544, 884
Bme1390I CCNGG 2 cut(s) 417, 927
BmeRI GACNNNNNGTC 2 cut(s) 173, 480
BmeT110I CYCGRG 2 cut(s) 293, 644
BmgT120I GGNCC 2 cut(s) 282, 1531
BmiI GGNNCC 2 cut(s) 255, 1532
BmrFI CCNGG 2 cut(s) 417, 927
BmrI ACTGGG 1 cut(s) 893
BmsI GCATC 4 cut(s) 38, 403, 964, 1286
BmtI GCTAGC 1 cut(s) 1055
BmuI ACTGGG 1 cut(s) 893
BoxI GACNNNNGTC 2 cut(s) 211, 508
BpiI GAAGAC 2 cut(s) 504, 611
BplI GAGNNNNNCTC 4 cut(s) 80, 112, 563, 595
BpmI CTGGAG 1 cut(s) 565
Bpu10I CCTNAGC 1 cut(s) 66
Bpu1102I GCTNAGC 1 cut(s) 1056
BpuEI CTTGAG 2 cut(s) 1006, 1245
BsaJI CCNNGG 7 cut(s) 77, 233, 415, 416, 468, 645, 1144
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 228, 422
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 4 cut(s) 582, 675, 811, 899
Bse3DI GCAATG 1 cut(s) 1356
BseBI CCWGG 2 cut(s) 417, 927
BseDI CCNNGG 7 cut(s) 77, 233, 415, 416, 468, 645, 1144
BseGI GGATG 2 cut(s) 61, 215
BseLI CCNNNNNNNGG 3 cut(s) 119, 228, 422
BseMI GCAATG 1 cut(s) 1356
BseMII CTCAG 5 cut(s) 80, 561, 1062, 1070, 1359
BseNI ACTGG 4 cut(s) 582, 675, 811, 899
BseRI GAGGAG 5 cut(s) 291, 384, 387, 390, 625
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 4 cut(s) 62, 65, 251, 554
Bsh1285I CGRYCG 1 cut(s) 690
BshFI GGCC 9 cut(s) 76, 119, 232, 283, 575, 650, 709, 885, 1533
BsiEI CGRYCG 1 cut(s) 690
BsiHKAI GWGCWC 2 cut(s) 98, 801
BsiHKCI CYCGRG 2 cut(s) 293, 644
BsiSI CCGG 2 cut(s) 120, 1285
BslFI GGGAC 5 cut(s) 180, 323, 329, 335, 887
BslI CCNNNNNNNGG 3 cut(s) 119, 228, 422
BsmAI GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
BsmFI GGGAC 5 cut(s) 180, 323, 329, 335, 887
BsmI GAATGC 1 cut(s) 1279
BsnI GGCC 9 cut(s) 76, 119, 232, 283, 575, 650, 709, 885, 1533
BsoBI CYCGRG 2 cut(s) 293, 644
Bsp1286I GDGCHC 3 cut(s) 38, 98, 801
Bsp143I GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
Bsp1720I GCTNAGC 1 cut(s) 1056
Bsp19I CCATGG 1 cut(s) 468
BspACI CCGC 9 cut(s) 70, 73, 262, 285, 289, 308, 508, 882, 1003
BspANI GGCC 9 cut(s) 76, 119, 232, 283, 575, 650, 709, 885, 1533
BspCNI CTCAG 5 cut(s) 79, 562, 1063, 1069, 1358
BspLI GGNNCC 2 cut(s) 255, 1532
BspOI GCTAGC 1 cut(s) 1055
BspPI GGATC 4 cut(s) 12, 119, 1215, 1437
BspTI CTTAAG 1 cut(s) 1525
BsrDI GCAATG 1 cut(s) 1356
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 4 cut(s) 582, 675, 811, 899
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 7 cut(s) 77, 233, 415, 416, 468, 645, 1144
BssMI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
BssT1I CCWWGG 3 cut(s) 77, 468, 1144
Bst2UI CCWGG 2 cut(s) 417, 927
Bst4CI ACNGT 4 cut(s) 670, 721, 786, 1559
Bst6I CTCTTC 6 cut(s) 153, 812, 1121, 1227, 1326, 1509
BstAFI CTTAAG 1 cut(s) 1525
BstAPI GCANNNNNTGC 1 cut(s) 1393
BstC8I GCNNGC 3 cut(s) 285, 289, 1053
BstDEI CTNAG 6 cut(s) 66, 549, 570, 1056, 1071, 1345
BstDSI CCRYGG 2 cut(s) 233, 468
BstENI CCTNNNNNAGG 1 cut(s) 420
BstF5I GGATG 2 cut(s) 61, 215
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 6 cut(s) 7, 127, 143, 316, 1210, 1432
BstMAI GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
BstMBI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
BstMCI CGRYCG 1 cut(s) 690
BstMWI GCNNNNNNNGC 6 cut(s) 238, 542, 1057, 1262, 1393, 1530
BstNI CCWGG 2 cut(s) 417, 927
BstPAI GACNNNNGTC 2 cut(s) 211, 508
BstSCI CCNGG 2 cut(s) 415, 925
BstSLI GKGCMC 1 cut(s) 38
BstV1I GCAGC 4 cut(s) 62, 65, 251, 554
BstV2I GAAGAC 2 cut(s) 504, 611
BstX2I RGATCY 1 cut(s) 1429
BstYI RGATCY 1 cut(s) 1429
BsuRI GGCC 9 cut(s) 76, 119, 232, 283, 575, 650, 709, 885, 1533
BtgI CCRYGG 2 cut(s) 233, 468
BtsCI GGATG 2 cut(s) 61, 215
BtsI GCAGTG 3 cut(s) 832, 876, 1019
BtsIMutI CAGTG 9 cut(s) 567, 589, 782, 832, 876, 1019, 1217, 1337, 1555
Cac8I GCNNGC 3 cut(s) 285, 289, 1053
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 2 cut(s) 282, 1531
CseI GACGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 193
CspCI CAANNNNNGTGG 2 cut(s) 1491, 1526
CviAII CATG 6 cut(s) 106, 469, 640, 909, 1048, 1379
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 6 cut(s) 66, 549, 570, 1056, 1071, 1345
DpnI GATC 6 cut(s) 6, 126, 142, 315, 1209, 1431
DpnII GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
DraI TTTAAA 1 cut(s) 358
DraIII CACNNNGTG 1 cut(s) 911
DriI GACNNNNNGTC 2 cut(s) 173, 480
EaeI YGGCCR 2 cut(s) 74, 117
Eam1104I CTCTTC 6 cut(s) 153, 812, 1121, 1227, 1326, 1509
Eam1105I GACNNNNNGTC 2 cut(s) 173, 480
EarI CTCTTC 6 cut(s) 153, 812, 1121, 1227, 1326, 1509
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 3 cut(s) 77, 468, 1144
Eco147I AGGCCT 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 293, 644
EcoICRI GAGCTC 1 cut(s) 96
EcoNI CCTNNNNNAGG 1 cut(s) 420
EcoRII CCWGG 2 cut(s) 415, 925
EcoT14I CCWWGG 3 cut(s) 77, 468, 1144
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 3 cut(s) 77, 468, 1144
FaeI CATG 6 cut(s) 109, 472, 643, 912, 1051, 1382
FaqI GGGAC 5 cut(s) 180, 323, 329, 335, 887
FatI CATG 6 cut(s) 105, 468, 639, 908, 1047, 1378
FauI CCCGC 2 cut(s) 292, 296
FauNDI CATATG 1 cut(s) 859
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 308, 543, 883
FokI GGATG 2 cut(s) 48, 202
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 308, 543, 883
FspBI CTAG 7 cut(s) 99, 480, 531, 915, 960, 1052, 1433
GlaI GCGC 1 cut(s) 49
GluI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 308, 543, 883
GsuI CTGGAG 1 cut(s) 565
HaeIII GGCC 9 cut(s) 76, 119, 232, 283, 575, 650, 709, 885, 1533
HapII CCGG 2 cut(s) 120, 1285
HgaI GACGC 1 cut(s) 320
HhaI GCGC 1 cut(s) 50
Hin1II CATG 6 cut(s) 109, 472, 643, 912, 1051, 1382
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HindIII AAGCTT 1 cut(s) 1263
HinfI GANTC 4 cut(s) 296, 498, 839, 1118
HpaII CCGG 2 cut(s) 120, 1285
HphI GGTGA 7 cut(s) 53, 94, 103, 134, 135, 484, 598
Hpy166II GTNNAC 1 cut(s) 680
Hpy188I TCNGA 6 cut(s) 271, 503, 796, 934, 968, 1429
Hpy188III TCNNGA 5 cut(s) 453, 485, 623, 1184, 1226
Hpy8I GTNNAC 1 cut(s) 680
HpyAV CCTTC 2 cut(s) 606, 1393
HpyCH4III ACNGT 4 cut(s) 670, 721, 786, 1559
HpyF10VI GCNNNNNNNGC 6 cut(s) 238, 542, 1057, 1262, 1393, 1530
HpyF3I CTNAG 6 cut(s) 66, 549, 570, 1056, 1071, 1345
Hsp92II CATG 6 cut(s) 109, 472, 643, 912, 1051, 1382
HspAI GCGC 1 cut(s) 48
Kzo9I GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
LmnI GCTCC 4 cut(s) 253, 638, 767, 797
Lsp1109I GCAGC 4 cut(s) 62, 65, 251, 554
LweI GCATC 4 cut(s) 38, 403, 964, 1286
MaeI CTAG 7 cut(s) 99, 480, 531, 915, 960, 1052, 1433
MaeIII GTNAC 5 cut(s) 472, 526, 584, 701, 827
MalI GATC 6 cut(s) 6, 126, 142, 315, 1209, 1431
MboI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 1 cut(s) 1429
MhlI GDGCHC 3 cut(s) 38, 98, 801
MluCI AATT 8 cut(s) 8, 517, 743, 833, 1179, 1194, 1239, 1539
MlyI GAGTC 1 cut(s) 305
Mph1103I ATGCAT 1 cut(s) 1279
MseI TTAA 4 cut(s) 357, 1097, 1308, 1526
MslI CAYNNNNRTG 1 cut(s) 467
MspA1I CMGCKG 3 cut(s) 70, 264, 508
MspCI CTTAAG 1 cut(s) 1525
MspI CCGG 2 cut(s) 120, 1285
MspR9I CCNGG 2 cut(s) 417, 927
MunI CAATTG 1 cut(s) 8
Mva1269I GAATGC 1 cut(s) 1279
MvaI CCWGG 2 cut(s) 417, 927
MwoI GCNNNNNNNGC 6 cut(s) 238, 542, 1057, 1262, 1393, 1530
NcoI CCATGG 1 cut(s) 468
NdeI CATATG 1 cut(s) 859
NdeII GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
NheI GCTAGC 1 cut(s) 1051
NlaIII CATG 6 cut(s) 109, 472, 643, 912, 1051, 1382
NlaIV GGNNCC 2 cut(s) 255, 1532
NmuCI GTSAC 4 cut(s) 472, 526, 584, 827
NsiI ATGCAT 1 cut(s) 1279
PasI CCCWGGG 1 cut(s) 416
PceI AGGCCT 1 cut(s) 709
PctI GAATGC 1 cut(s) 1279
PfeI GAWTC 3 cut(s) 498, 839, 1118
PkrI GCNGC 8 cut(s) 52, 55, 72, 75, 266, 309, 544, 884
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PshAI GACNNNNGTC 2 cut(s) 211, 508
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 415, 925
PspGI CCWGG 2 cut(s) 415, 925
PspN4I GGNNCC 2 cut(s) 255, 1532
PspPI GGNCC 2 cut(s) 282, 1531
PsuI RGATCY 1 cut(s) 1429
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 467
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 4 cut(s) 357, 1097, 1308, 1526
SatI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 308, 543, 883
Sau3AI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
Sau96I GGNCC 2 cut(s) 282, 1531
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 417, 927
SduI GDGCHC 3 cut(s) 38, 98, 801
SfaNI GCATC 4 cut(s) 38, 403, 964, 1286
SmiMI CAYNNNNRTG 1 cut(s) 467
SmlI CTYRAG 3 cut(s) 985, 1260, 1525
SmoI CTYRAG 3 cut(s) 985, 1260, 1525
SpeI ACTAGT 1 cut(s) 530
Sse9I AATT 8 cut(s) 8, 517, 743, 833, 1179, 1194, 1239, 1539
SseBI AGGCCT 1 cut(s) 709
SsiI CCGC 9 cut(s) 70, 73, 262, 285, 289, 308, 508, 882, 1003
SspMI CTAG 7 cut(s) 99, 480, 531, 915, 960, 1052, 1433
SstI GAGCTC 1 cut(s) 98
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 2 cut(s) 415, 925
StyI CCWWGG 3 cut(s) 77, 468, 1144
TaaI ACNGT 4 cut(s) 670, 721, 786, 1559
TaqI TCGA 1 cut(s) 486
TaqII GACCGA 1 cut(s) 704
TasI AATT 8 cut(s) 8, 517, 743, 833, 1179, 1194, 1239, 1539
TauI GCSGC 4 cut(s) 73, 76, 310, 885
TfiI GAWTC 3 cut(s) 498, 839, 1118
Tru1I TTAA 4 cut(s) 357, 1097, 1308, 1526
Tru9I TTAA 4 cut(s) 357, 1097, 1308, 1526
TscAI CASTG 9 cut(s) 574, 589, 789, 832, 876, 1019, 1217, 1344, 1562
TseFI GTSAC 4 cut(s) 472, 526, 584, 827
TseI GCWGC 4 cut(s) 50, 53, 264, 542
Tsp45I GTSAC 4 cut(s) 472, 526, 584, 827
TspDTI ATGAA 1 cut(s) 94
TspGWI ACGGA 4 cut(s) 211, 250, 381, 1150
TspRI CASTG 9 cut(s) 574, 589, 789, 832, 876, 1019, 1217, 1344, 1562
Vha464I CTTAAG 1 cut(s) 1525
XagI CCTNNNNNAGG 1 cut(s) 420
XapI RAATTY 2 cut(s) 743, 1194
XmiI GTMKAC 1 cut(s) 679
XspI CTAG 7 cut(s) 99, 480, 531, 915, 960, 1052, 1433
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.