Rorug04G0380700

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0005101
Physical Location & Seq
Reverse (-)
55130816 .. 55132851
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0380700.1

Sequence Viewer

Length: 1536 bp
ATGATCAGCTTTAGCCCGGTGATCATCGTTTCAAGCTTTGTTTCTGTGCTCCTTCTCTTAGCTGTCATCAAAATCTTTCACAAACTATGGTGGACTCCAACTCGCATACAGAATCTGATGGCTTTGCAAGGAATCAGAGGCCCTTCTTACAGACTTGTCCATGGGAATTCCAGAGAAATCAACAACATGAGAAAGGAAGCTATGAGCAGACCCAAGAGTTTATCACACAACATATTTCCTGAAGTTCATCCTCATATTCATTCATGGACCAAGACATATGGCAAAACATTTCTTCAGTGGCATGGTGGTAAAGCACAGTTGGTCATTATGGAACCTGAGCTGTGCAAAGAGATACTGAATGGAGCTTTTCCAAAACCGGAGACCAAACGTTTTGTAAAGAAGCTGTTAGGAGATGGCCTCACACGATCCGAAGGTGAAAAATGGGTAAAAATGCGAAAACTGGCTAACCATGCCTTCCATGGAGAGAGCTTAAGAACTATGATTCCGGCGATGATTACTAGCGCTGAGACGATGCTAGAAAGGTGGACAAGTCATAATGGCAAAGAGATTGAGGTGTTTGAAGAGTTTAGACTGTACACTTCAGAAGTGATTTCTAGGACAGCATTTGGAAGCAGCTATATAGAAGGAAAACAGATTTTTGAGATGTTAATAAAGATGGGATTCTTAACATTCAAAAATGCTTTCAACCTCAGGTTTCCTGGCATCAGTAAAATTTATAAGACCAGCGACGAGATTGAATCAGACAAGCTTGAAAAAGGAATAAGGGACTCCATAATAGAAATTGTTAAGAAAAGAGAAGAGACGGCAATGAGAAGACAAGAAGACTACTTTGGGAGTGATTTTCTTGGATTACTGCTAAAGGCTTATCATGATCCCAATGAGAGGCAGAGGATTTCGATAGACGATTTAGTTGATGAGTGCAAGGCGTTTTACTTTGCTGGACAAGAAACTAGTACTAGTTTGCTTGCTTGGACTGTCTTTCTTCTGGCTCTCCATACTGATTGGCAAGAAGAAGCAAGAAATGAAGTCCTACAATTATTTGGCAAACAAACACCAAATCCTGATGGCTTTTCCAAACTGAAAACAATGAGTATGATCATCAATGAATCTCTAAGGTTATATCCTCCTGTTCTTGCTGTAGAGAGGAGAGTTGAAAAGGAAGTTAGACTGGGAAACCTCGTCATTCCTGCCAATGTTGAATTGCATATTTCAAGTCTAGCACTTCACCATGAGCCTCAATTCTGGGGACAAGATGTGCAACTTTTCAAGCCAGAGCGATTCTCAGAAGGGGTTGCTAAGGCTACTGGGAATAACATAGTCGCATTCTTACCATTTGGAATGGGGCCTCGAACTTGTGTGGGCTTCAACTTTGCCACCGTTGAAGTAAAGATTGCTCTTTCAATGATTCTACAACGCTACTCGTTCACCCTTTCACCGGGTTATGTTCACTCTCCCTTTCATTTTCTTACACTTCGTCCACAGCATGGAGTTCAAGTAATGCTACAGACACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

511

Amino Acids

58.65

Weight (kDa)

9.2

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 82 - 492 6.9e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 738
AccB7I CCANNNNNTGG 1 cut(s) 1505
AclI AACGTT 1 cut(s) 388
AclWI GGATC 2 cut(s) 420, 887
AcsI RAATTY 2 cut(s) 166, 732
AcuI CTGAAG 3 cut(s) 261, 278, 585
AfaI GTAC 2 cut(s) 596, 976
AfeI AGCGCT 1 cut(s) 523
AfiI CCNNNNNNNGG 3 cut(s) 903, 1456, 1505
AflII CTTAAG 1 cut(s) 490
AhlI ACTAGT 2 cut(s) 971, 977
AjnI CCWGG 1 cut(s) 718
AjuI GAANNNNNNNTTGG 2 cut(s) 834, 866
AloI GAACNNNNNNTCC 2 cut(s) 487, 519
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 3 cut(s) 374, 521, 815
AlwI GGATC 2 cut(s) 420, 887
AlwNI CAGNNNCTG 2 cut(s) 115, 1531
Aor51HI AGCGCT 1 cut(s) 523
AoxI GGCC 3 cut(s) 139, 415, 1364
ApeKI GCWGC 1 cut(s) 633
ApoI RAATTY 2 cut(s) 166, 732
AspLEI GCGC 1 cut(s) 524
AspS9I GGNCC 3 cut(s) 140, 267, 1364
AsuC2I CCSGG 2 cut(s) 17, 1458
AsuHPI GGTGA 5 cut(s) 31, 446, 1238, 1438, 1446
AvaII GGWCC 1 cut(s) 267
AxyI CCTNAGG 1 cut(s) 710
BbsI GAAGAC 2 cut(s) 841, 849
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 1 cut(s) 645
BccI CCATC 4 cut(s) 112, 407, 670, 1079
BceAI ACGGC 1 cut(s) 840
BciT130I CCWGG 1 cut(s) 720
BclI TGATCA 3 cut(s) 3, 21, 1116
BcnI CCSGG 2 cut(s) 17, 1458
BcoDI GTCTC 3 cut(s) 374, 521, 815
BcuI ACTAGT 2 cut(s) 971, 977
BfaI CTAG 6 cut(s) 519, 536, 615, 972, 978, 1238
BfmI CTRYAG 2 cut(s) 1158, 1523
BfoI RGCGCY 1 cut(s) 525
BfrI CTTAAG 1 cut(s) 490
BisI GCNGC 1 cut(s) 634
BlsI GCNGC 1 cut(s) 635
BmcAI AGTACT 1 cut(s) 976
Bme1390I CCNGG 3 cut(s) 17, 720, 1458
Bme18I GGWCC 1 cut(s) 267
BmgT120I GGNCC 3 cut(s) 140, 267, 1364
BmiI GGNNCC 2 cut(s) 333, 1365
BmrFI CCNGG 3 cut(s) 17, 720, 1458
BmrI ACTGGG 2 cut(s) 1199, 1335
BmsI GCATC 2 cut(s) 522, 732
BmuI ACTGGG 2 cut(s) 1199, 1335
BpiI GAAGAC 2 cut(s) 841, 849
BplI GAGNNNNNCTC 4 cut(s) 402, 434, 1286, 1318
Bpu10I CCTNAGC 2 cut(s) 336, 1317
BpuMI CCSGG 2 cut(s) 17, 1458
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 160, 478
BsaWI WCCGGW 1 cut(s) 376
BsaXI ACNNNNNCTCC 2 cut(s) 1500, 1530
Bsc4I CCNNNNNNNGG 3 cut(s) 903, 1456, 1505
Bse1I ACTGG 3 cut(s) 465, 1194, 1330
Bse21I CCTNAGG 1 cut(s) 710
Bse3DI GCAATG 1 cut(s) 834
BseBI CCWGG 1 cut(s) 720
BseDI CCNNGG 2 cut(s) 160, 478
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 3 cut(s) 903, 1456, 1505
BseMI GCAATG 1 cut(s) 834
BseMII CTCAG 4 cut(s) 327, 516, 724, 1317
BseNI ACTGG 3 cut(s) 465, 1194, 1330
BseRI GAGGAG 1 cut(s) 1180
BseXI GCAGC 1 cut(s) 645
BshFI GGCC 3 cut(s) 141, 417, 1366
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 4 cut(s) 17, 377, 506, 1457
BslFI GGGAC 2 cut(s) 800, 1281
BslI CCNNNNNNNGG 3 cut(s) 903, 1456, 1505
BsmAI GTCTC 3 cut(s) 374, 521, 815
BsmBI CGTCTC 2 cut(s) 521, 815
BsmFI GGGAC 2 cut(s) 800, 1281
BsmI GAATGC 1 cut(s) 1343
BsnI GGCC 3 cut(s) 141, 417, 1366
Bso31I GGTCTC 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 51
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 5 cut(s) 3, 21, 425, 892, 1116
Bsp19I CCATGG 2 cut(s) 160, 478
BspANI GGCC 3 cut(s) 141, 417, 1366
BspCNI CTCAG 4 cut(s) 328, 517, 723, 1316
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 2 cut(s) 333, 1365
BspPI GGATC 2 cut(s) 420, 887
BspTI CTTAAG 1 cut(s) 490
BspTNI GGTCTC 1 cut(s) 374
BsrDI GCAATG 1 cut(s) 834
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 3 cut(s) 465, 1194, 1330
BssECI CCNNGG 2 cut(s) 160, 478
BssMI GATC 5 cut(s) 3, 21, 425, 892, 1116
BssT1I CCWWGG 2 cut(s) 160, 478
Bst2UI CCWGG 1 cut(s) 720
Bst4CI ACNGT 5 cut(s) 318, 594, 997, 1399, 1533
Bst6I CTCTTC 2 cut(s) 576, 813
BstAFI CTTAAG 1 cut(s) 490
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 1 cut(s) 987
BstDEI CTNAG 7 cut(s) 58, 336, 525, 710, 1133, 1303, 1317
BstDSI CCRYGG 2 cut(s) 160, 478
BstF5I GGATG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 525
BstHHI GCGC 1 cut(s) 524
BstKTI GATC 5 cut(s) 6, 24, 428, 895, 1119
BstMAI GTCTC 3 cut(s) 374, 521, 815
BstMBI GATC 5 cut(s) 3, 21, 425, 892, 1116
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstNI CCWGG 1 cut(s) 720
BstSCI CCNGG 3 cut(s) 15, 718, 1456
BstSFI CTRYAG 2 cut(s) 1158, 1523
BstV1I GCAGC 1 cut(s) 645
BstV2I GAAGAC 2 cut(s) 841, 849
Bsu36I CCTNAGG 1 cut(s) 710
BsuRI GGCC 3 cut(s) 141, 417, 1366
BtgI CCRYGG 2 cut(s) 160, 478
BtgZI GCGATG 1 cut(s) 524
BtsCI GGATG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 302, 1529
Cac8I GCNNGC 1 cut(s) 987
CaiI CAGNNNCTG 2 cut(s) 115, 1531
CciI TCATGA 1 cut(s) 889
CfoI GCGC 1 cut(s) 524
Cfr13I GGNCC 3 cut(s) 140, 267, 1364
Csp6I GTAC 2 cut(s) 595, 975
CviAII CATG 9 cut(s) 161, 187, 264, 302, 470, 479, 890, 1250, 1505
CviQI GTAC 2 cut(s) 595, 975
DdeI CTNAG 7 cut(s) 58, 336, 525, 710, 1133, 1303, 1317
DpnI GATC 5 cut(s) 5, 23, 427, 894, 1118
DpnII GATC 5 cut(s) 3, 21, 425, 892, 1116
Eam1104I CTCTTC 2 cut(s) 576, 813
EarI CTCTTC 2 cut(s) 576, 813
Eco130I CCWWGG 2 cut(s) 160, 478
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 267
Eco47III AGCGCT 1 cut(s) 523
Eco57I CTGAAG 3 cut(s) 261, 278, 585
Eco81I CCTNAGG 1 cut(s) 710
EcoO109I RGGNCCY 2 cut(s) 140, 1364
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 160, 478
ErhI CCWWGG 2 cut(s) 160, 478
Esp3I CGTCTC 2 cut(s) 521, 815
FaeI CATG 9 cut(s) 164, 190, 267, 305, 473, 482, 893, 1253, 1508
FaqI GGGAC 2 cut(s) 800, 1281
FatI CATG 9 cut(s) 160, 186, 263, 301, 469, 478, 889, 1249, 1504
FauNDI CATATG 1 cut(s) 277
FbaI TGATCA 3 cut(s) 3, 21, 1116
Fnu4HI GCNGC 1 cut(s) 634
FokI GGATG 1 cut(s) 234
Fsp4HI GCNGC 1 cut(s) 634
FspBI CTAG 6 cut(s) 519, 536, 615, 972, 978, 1238
GlaI GCGC 1 cut(s) 523
GluI GCNGC 1 cut(s) 634
HaeII RGCGCY 1 cut(s) 525
HaeIII GGCC 3 cut(s) 141, 417, 1366
HapII CCGG 4 cut(s) 17, 377, 506, 1457
HhaI GCGC 1 cut(s) 524
Hin1II CATG 9 cut(s) 164, 190, 267, 305, 473, 482, 893, 1253, 1508
Hin6I GCGC 1 cut(s) 522
HinP1I GCGC 1 cut(s) 522
HindIII AAGCTT 2 cut(s) 34, 767
HpaII CCGG 4 cut(s) 17, 377, 506, 1457
HphI GGTGA 5 cut(s) 31, 446, 1238, 1438, 1446
Hpy166II GTNNAC 6 cut(s) 93, 546, 597, 1446, 1468, 1499
Hpy188I TCNGA 6 cut(s) 117, 137, 430, 604, 763, 1306
Hpy188III TCNNGA 4 cut(s) 171, 239, 890, 1082
Hpy8I GTNNAC 6 cut(s) 93, 546, 597, 1446, 1468, 1499
Hpy99I CGWCG 1 cut(s) 752
HpyAV CCTTC 6 cut(s) 62, 153, 425, 484, 638, 1301
HpyCH4III ACNGT 5 cut(s) 318, 594, 997, 1399, 1533
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 5 cut(s) 127, 345, 942, 1225, 1279
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 7 cut(s) 58, 336, 525, 710, 1133, 1303, 1317
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 9 cut(s) 164, 190, 267, 305, 473, 482, 893, 1253, 1508
HspAI GCGC 1 cut(s) 522
Ksp22I TGATCA 3 cut(s) 3, 21, 1116
Kzo9I GATC 5 cut(s) 3, 21, 425, 892, 1116
LmnI GCTCC 2 cut(s) 54, 362
Lsp1109I GCAGC 1 cut(s) 645
LweI GCATC 2 cut(s) 522, 732
MaeI CTAG 6 cut(s) 519, 536, 615, 972, 978, 1238
MaeII ACGT 1 cut(s) 388
MalI GATC 5 cut(s) 5, 23, 427, 894, 1118
MboI GATC 5 cut(s) 3, 21, 425, 892, 1116
MboII GAAGA 7 cut(s) 284, 593, 830, 846, 854, 995, 1043
MhlI GDGCHC 1 cut(s) 51
MluCI AATT 6 cut(s) 166, 732, 801, 1055, 1220, 1259
MlyI GAGTC 2 cut(s) 88, 782
MmeI TCCRAC 1 cut(s) 122
MseI TTAA 4 cut(s) 491, 668, 686, 807
MspCI CTTAAG 1 cut(s) 490
MspI CCGG 4 cut(s) 17, 377, 506, 1457
MspR9I CCNGG 3 cut(s) 17, 720, 1458
Mva1269I GAATGC 1 cut(s) 1343
MvaI CCWGG 1 cut(s) 720
MwoI GCNNNNNNNGC 1 cut(s) 470
NciI CCSGG 2 cut(s) 17, 1458
NcoI CCATGG 2 cut(s) 160, 478
NdeI CATATG 1 cut(s) 277
NdeII GATC 5 cut(s) 3, 21, 425, 892, 1116
NlaIII CATG 9 cut(s) 164, 190, 267, 305, 473, 482, 893, 1253, 1508
NlaIV GGNNCC 2 cut(s) 333, 1365
PagI TCATGA 1 cut(s) 889
PctI GAATGC 1 cut(s) 1343
PfeI GAWTC 8 cut(s) 112, 132, 502, 681, 758, 1127, 1299, 1426
PflMI CCANNNNNTGG 1 cut(s) 1505
PkrI GCNGC 1 cut(s) 635
PleI GAGTC 2 cut(s) 88, 782
PpsI GAGTC 2 cut(s) 88, 782
PsiI TTATAA 1 cut(s) 738
Psp1406I AACGTT 1 cut(s) 388
Psp6I CCWGG 1 cut(s) 718
PspGI CCWGG 1 cut(s) 718
PspN4I GGNNCC 2 cut(s) 333, 1365
PspPI GGNCC 3 cut(s) 140, 267, 1364
PstNI CAGNNNCTG 2 cut(s) 115, 1531
RsaI GTAC 2 cut(s) 596, 976
RsaNI GTAC 2 cut(s) 595, 975
SaqAI TTAA 4 cut(s) 491, 668, 686, 807
SatI GCNGC 1 cut(s) 634
Sau3AI GATC 5 cut(s) 3, 21, 425, 892, 1116
Sau96I GGNCC 3 cut(s) 140, 267, 1364
ScaI AGTACT 1 cut(s) 976
SchI GAGTC 2 cut(s) 88, 782
ScrFI CCNGG 3 cut(s) 17, 720, 1458
SduI GDGCHC 1 cut(s) 51
SfaNI GCATC 2 cut(s) 522, 732
SfcI CTRYAG 2 cut(s) 1158, 1523
SinI GGWCC 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 490
SmoI CTYRAG 1 cut(s) 490
SpeI ACTAGT 2 cut(s) 971, 977
Sse9I AATT 6 cut(s) 166, 732, 801, 1055, 1220, 1259
SspMI CTAG 6 cut(s) 519, 536, 615, 972, 978, 1238
StyD4I CCNGG 3 cut(s) 15, 718, 1456
StyI CCWWGG 2 cut(s) 160, 478
TaaI ACNGT 5 cut(s) 318, 594, 997, 1399, 1533
TaiI ACGT 1 cut(s) 391
TaqI TCGA 2 cut(s) 917, 1369
TasI AATT 6 cut(s) 166, 732, 801, 1055, 1220, 1259
TatI WGTACW 2 cut(s) 594, 974
TfiI GAWTC 8 cut(s) 112, 132, 502, 681, 758, 1127, 1299, 1426
Tru1I TTAA 4 cut(s) 491, 668, 686, 807
Tru9I TTAA 4 cut(s) 491, 668, 686, 807
TscAI CASTG 2 cut(s) 302, 1536
TseI GCWGC 1 cut(s) 633
TspDTI ATGAA 6 cut(s) 236, 248, 252, 1059, 1140, 1469
TspRI CASTG 2 cut(s) 302, 1536
Van91I CCANNNNNTGG 1 cut(s) 1505
Vha464I CTTAAG 1 cut(s) 490
VpaK11BI GGWCC 1 cut(s) 267
XapI RAATTY 2 cut(s) 166, 732
XcmI CCANNNNNNNNNTGG 1 cut(s) 476
XspI CTAG 6 cut(s) 519, 536, 615, 972, 978, 1238
ZrmI AGTACT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.