FvH4_3g00020

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0005101
Physical Location & Seq
Reverse (-)
21493 .. 26710
5218 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g00020.t1

Sequence Viewer

Length: 1407 bp
ATGGATCAATTGATGGTGGAAAACTTGGCATCTGGCACGGGTGAGGGAGCAGTAGCCCCTCATCCTCCGCGGCGGCCAAGGGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGTGGCCAGTGATCCCTTCCCCACCAGATCACCTCTTCCCAGACATCAAAATCAACAGGGTGTCTCAACTCCAAGCCAGCGGTCACGGAGGCACCTGGACTTGGAGCCCTTCCGCTGCTCCGACGAGAACAGGCCCACCCGCGCCGAGTCTCCTCTCCCACCGGATCAGCAGGCAAGGAAACAGCGTCCTGTCAATGCCAATGCCAAGCCTTTGAAAGAGAACGGAGGAGGCTCAAGACCCGACACGCCCATGGTGACTGCTAGTCTCGATAGGACTCCGTCAAGACCCAGACTCATTCAGCAACGGGTCAGTGCCACTGCAGCTGCTAAGCTCTTGAAAACTACCACCAGCGAGGCCAATATCCAGGAAGCCACCTCCAGGTGTCTTTCTGACATCCGCTCCTCTATGCCCGAATCCAATATGTTGCCAACTGTTTCCAGTAGACAGACGAGTGAAAAAAGTTGTAACAATGGCGGTGGTTTTTCAAAAATTTCTGCTTCTCCTTGTTCCCGTTCTCTCAACTTGCCACTGTCAAGCTCCGAGCACTTACACTTCCAGTCCGTCAAAGGCAGTGAAAAATTAGCATCTGCTTTGTCCAAGCCATGTACAAATGCAGTGAATACGGGCGGCCTCTGTCTGCCCCCAGTCCCACCATGTGCTACTGCGAAGCCAGCTTCAGAATTTAGGAAAGGAAAGAAGGAAGATGTTCATTCCTTGAGATTGCTTCATAACCGCTATTTGCAGTGGAGATATGCCAATGCAACAGCAGAGGCTTCCTTGCGAGCTCAGCAGAGAGAGACTGAGAGAACGCTTTATTCTCTTGCATTACAGATAACAGAACTATATGATTCTGTGAAGAGAAAACGGATAGAACTTGGCGTTTTGCAAAGAACAAAGAATTTATCGACAATTCTTGAAGCGCAAATTCCATATTTGGATCAGTGGTTTTCTCTTAAAGAGGACTATTTAATTTCTCTGGCAGAAGCAACACAAGCTTTGTTGAACACATCACTTCGGCTTCCAATCAGTGCCAATGTTAAGGCTAATAAACAAGAGGTAGAAGAGGCACTGAACTCAGCAATGGAAGTCATTGAGAAAATAGTTTTCCATGTTCAGCAATCTATGCCAAAGGCAGAGGAAACAGACCATTTAATTTCAGAACTAGCAAGAGTGGCTGGTGGAGAAAGAACTCTCGTTGAAGTAAGTGGAAATTTGTTGTCCAAGACATATACGACACAGGTGGAAGAGTGTAGCTTAAGGGGTCATATAATCCAGTTGAAACAACATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

469

Amino Acids

51.73

Weight (kDa)

9.46

Isoelectric Point (pI)

62.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 143 - 437 2e-52 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 204
AccBSI CCGCTC 1 cut(s) 513
AccI GTMKAC 1 cut(s) 556
AccII CGCG 2 cut(s) 70, 255
AclWI GGATC 4 cut(s) 12, 119, 285, 1059
AcoI YGGCCR 2 cut(s) 74, 117
AcsI RAATTY 5 cut(s) 603, 794, 1012, 1038, 1324
AcuI CTGAAG 1 cut(s) 774
AdeI CACNNNGTG 1 cut(s) 770
AfaI GTAC 1 cut(s) 721
AfiI CCNNNNNNNGG 2 cut(s) 214, 492
AflII CTTAAG 1 cut(s) 1369
AgsI TTSAA 7 cut(s) 328, 451, 600, 1031, 1117, 1313, 1393
AhdI GACNNNNNGTC 1 cut(s) 375
AjnI CCWGG 3 cut(s) 207, 477, 491
AluBI AGCT 8 cut(s) 96, 437, 445, 651, 788, 899, 1109, 1368
AluI AGCT 8 cut(s) 96, 437, 445, 651, 788, 899, 1109, 1368
Alw21I GWGCWC 3 cut(s) 98, 660, 901
Alw26I GTCTC 5 cut(s) 114, 181, 267, 383, 905
AlwI GGATC 4 cut(s) 12, 119, 285, 1059
AoxI GGCC 5 cut(s) 74, 117, 245, 468, 742
ApeKI GCWGC 3 cut(s) 228, 434, 437
ApoI RAATTY 5 cut(s) 603, 794, 1012, 1038, 1324
Asp700I GAANNNNTTC 1 cut(s) 819
AspLEI GCGC 2 cut(s) 257, 1036
AspS9I GGNCC 1 cut(s) 246
AsuHPI GGTGA 5 cut(s) 53, 94, 103, 135, 379
BalI TGGCCA 1 cut(s) 119
BanI GGYRCC 1 cut(s) 204
BanII GRGCYC 3 cut(s) 98, 222, 901
Bbv12I GWGCWC 3 cut(s) 98, 660, 901
BbvI GCAGC 3 cut(s) 215, 424, 446
BccI CCATC 1 cut(s) 7
BciT130I CCWGG 3 cut(s) 209, 479, 493
BcoDI GTCTC 5 cut(s) 114, 181, 267, 383, 905
BfaI CTAG 3 cut(s) 99, 375, 1277
BfmI CTRYAG 1 cut(s) 432
BfrI CTTAAG 1 cut(s) 1369
BisI GCNGC 6 cut(s) 71, 74, 229, 435, 438, 742
BlpI GCTNAGC 2 cut(s) 441, 900
BlsI GCNGC 6 cut(s) 72, 75, 230, 436, 439, 743
Bme1390I CCNGG 3 cut(s) 209, 479, 493
BmeRI GACNNNNNGTC 1 cut(s) 375
BmgT120I GGNCC 1 cut(s) 246
BmiI GGNNCC 2 cut(s) 206, 219
BmrFI CCNGG 3 cut(s) 209, 479, 493
BmrI ACTGGG 1 cut(s) 752
BmsI GCATC 2 cut(s) 38, 707
BmuI ACTGGG 1 cut(s) 752
BplI GAGNNNNNCTC 2 cut(s) 80, 112
BpmI CTGGAG 1 cut(s) 475
Bpu1102I GCTNAGC 2 cut(s) 441, 900
BpuEI CTTGAG 2 cut(s) 331, 850
BsaJI CCNNGG 3 cut(s) 68, 77, 363
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 492
Bse1I ACTGG 5 cut(s) 120, 552, 670, 758, 1387
Bse3DI GCAATG 1 cut(s) 1200
BseBI CCWGG 3 cut(s) 209, 479, 493
BseDI CCNNGG 3 cut(s) 68, 77, 363
BseGI GGATG 2 cut(s) 61, 507
BseLI CCNNNNNNNGG 2 cut(s) 214, 492
BseMI GCAATG 1 cut(s) 1200
BseMII CTCAG 3 cut(s) 906, 914, 1203
BseNI ACTGG 5 cut(s) 120, 552, 670, 758, 1387
BseRI GAGGAG 3 cut(s) 255, 354, 505
BseXI GCAGC 3 cut(s) 215, 424, 446
Bsh1236I CGCG 2 cut(s) 70, 255
BshFI GGCC 5 cut(s) 76, 119, 247, 470, 744
BshNI GGYRCC 1 cut(s) 204
BsiHKAI GWGCWC 3 cut(s) 98, 660, 901
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 1 cut(s) 746
BslI CCNNNNNNNGG 2 cut(s) 214, 492
BsmAI GTCTC 5 cut(s) 114, 181, 267, 383, 905
BsmFI GGGAC 1 cut(s) 746
BsnI GGCC 5 cut(s) 76, 119, 247, 470, 744
Bsp1286I GDGCHC 4 cut(s) 98, 222, 660, 901
Bsp1407I TGTACA 1 cut(s) 719
Bsp143I GATC 5 cut(s) 4, 124, 140, 277, 1051
Bsp1720I GCTNAGC 2 cut(s) 441, 900
Bsp19I CCATGG 1 cut(s) 363
BspANI GGCC 5 cut(s) 76, 119, 247, 470, 744
BspCNI CTCAG 3 cut(s) 907, 913, 1202
BspFNI CGCG 2 cut(s) 70, 255
BspLI GGNNCC 2 cut(s) 206, 219
BspMAI CTGCAG 1 cut(s) 436
BspPI GGATC 4 cut(s) 12, 119, 285, 1059
BspT107I GGYRCC 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 1369
BsrBI CCGCTC 1 cut(s) 513
BsrDI GCAATG 1 cut(s) 1200
BsrGI TGTACA 1 cut(s) 719
BsrI ACTGG 5 cut(s) 120, 552, 670, 758, 1387
BssECI CCNNGG 3 cut(s) 68, 77, 363
BssMI GATC 5 cut(s) 4, 124, 140, 277, 1051
BssT1I CCWWGG 2 cut(s) 77, 363
Bst2UI CCWGG 3 cut(s) 209, 479, 493
Bst4CI ACNGT 2 cut(s) 547, 645
Bst6I CTCTTC 4 cut(s) 153, 965, 1170, 1353
BstAFI CTTAAG 1 cut(s) 1369
BstAPI GCANNNNNTGC 1 cut(s) 1237
BstAUI TGTACA 1 cut(s) 719
BstC8I GCNNGC 4 cut(s) 191, 285, 786, 897
BstDEI CTNAG 4 cut(s) 441, 900, 915, 1189
BstDSI CCRYGG 2 cut(s) 68, 363
BstF5I GGATG 2 cut(s) 61, 507
BstFNI CGCG 2 cut(s) 70, 255
BstHHI GCGC 2 cut(s) 257, 1036
BstKTI GATC 5 cut(s) 7, 127, 143, 280, 1054
BstMAI GTCTC 5 cut(s) 114, 181, 267, 383, 905
BstMBI GATC 5 cut(s) 4, 124, 140, 277, 1051
BstMWI GCNNNNNNNGC 6 cut(s) 434, 785, 901, 1106, 1237, 1286
BstNI CCWGG 3 cut(s) 209, 479, 493
BstSCI CCNGG 3 cut(s) 207, 477, 491
BstSFI CTRYAG 1 cut(s) 432
BstUI CGCG 2 cut(s) 70, 255
BstV1I GCAGC 3 cut(s) 215, 424, 446
BsuRI GGCC 5 cut(s) 76, 119, 247, 470, 744
BtgI CCRYGG 2 cut(s) 68, 363
BtsCI GGATG 2 cut(s) 61, 507
BtsI GCAGTG 4 cut(s) 429, 691, 735, 863
Cac8I GCNNGC 4 cut(s) 191, 285, 786, 897
CfoI GCGC 2 cut(s) 257, 1036
Cfr13I GGNCC 1 cut(s) 246
Cfr42I CCGCGG 1 cut(s) 71
CseI GACGC 1 cut(s) 287
Csp6I GTAC 1 cut(s) 720
CspCI CAANNNNNGTGG 2 cut(s) 571, 606
CviAII CATG 5 cut(s) 106, 364, 717, 768, 1223
CviQI GTAC 1 cut(s) 720
DdeI CTNAG 4 cut(s) 441, 900, 915, 1189
DpnI GATC 5 cut(s) 6, 126, 142, 279, 1053
DpnII GATC 5 cut(s) 4, 124, 140, 277, 1051
DraIII CACNNNGTG 1 cut(s) 770
DriI GACNNNNNGTC 1 cut(s) 375
EaeI YGGCCR 2 cut(s) 74, 117
Eam1104I CTCTTC 4 cut(s) 153, 965, 1170, 1353
Eam1105I GACNNNNNGTC 1 cut(s) 375
EarI CTCTTC 4 cut(s) 153, 965, 1170, 1353
Ecl136II GAGCTC 2 cut(s) 96, 899
Eco130I CCWWGG 2 cut(s) 77, 363
Eco24I GRGCYC 3 cut(s) 98, 222, 901
Eco53kI GAGCTC 2 cut(s) 96, 899
Eco57I CTGAAG 1 cut(s) 774
EcoICRI GAGCTC 2 cut(s) 96, 899
EcoRII CCWGG 3 cut(s) 207, 477, 491
EcoT14I CCWWGG 2 cut(s) 77, 363
EcoT38I GRGCYC 3 cut(s) 98, 222, 901
ErhI CCWWGG 2 cut(s) 77, 363
FaeI CATG 5 cut(s) 109, 367, 720, 771, 1226
FaqI GGGAC 1 cut(s) 746
FatI CATG 5 cut(s) 105, 363, 716, 767, 1222
FauI CCCGC 1 cut(s) 260
FblI GTMKAC 1 cut(s) 556
Fnu4HI GCNGC 6 cut(s) 71, 74, 229, 435, 438, 742
FokI GGATG 2 cut(s) 48, 494
FriOI GRGCYC 3 cut(s) 98, 222, 901
Fsp4HI GCNGC 6 cut(s) 71, 74, 229, 435, 438, 742
FspBI CTAG 3 cut(s) 99, 375, 1277
GlaI GCGC 2 cut(s) 256, 1035
GluI GCNGC 6 cut(s) 71, 74, 229, 435, 438, 742
GsuI CTGGAG 1 cut(s) 475
HaeIII GGCC 5 cut(s) 76, 119, 247, 470, 744
HapII CCGG 1 cut(s) 275
HgaI GACGC 1 cut(s) 287
HhaI GCGC 2 cut(s) 257, 1036
Hin1II CATG 5 cut(s) 109, 367, 720, 771, 1226
Hin6I GCGC 2 cut(s) 255, 1034
HinP1I GCGC 2 cut(s) 255, 1034
HindIII AAGCTT 1 cut(s) 1107
HinfI GANTC 5 cut(s) 260, 388, 405, 527, 962
HpaII CCGG 1 cut(s) 275
HphI GGTGA 5 cut(s) 53, 94, 103, 135, 379
Hpy166II GTNNAC 1 cut(s) 557
Hpy188I TCNGA 6 cut(s) 235, 505, 655, 793, 1273, 1406
Hpy188III TCNNGA 5 cut(s) 348, 380, 396, 448, 1028
Hpy8I GTNNAC 1 cut(s) 557
Hpy99I CGWCG 1 cut(s) 239
HpyAV CCTTC 3 cut(s) 139, 232, 805
HpyCH4III ACNGT 2 cut(s) 547, 645
HpyCH4V TGCA 6 cut(s) 434, 728, 856, 875, 938, 1000
HpyF10VI GCNNNNNNNGC 6 cut(s) 434, 785, 901, 1106, 1237, 1286
HpyF3I CTNAG 4 cut(s) 441, 900, 915, 1189
Hsp92II CATG 5 cut(s) 109, 367, 720, 771, 1226
HspAI GCGC 2 cut(s) 255, 1034
KspI CCGCGG 1 cut(s) 71
Kzo9I GATC 5 cut(s) 4, 124, 140, 277, 1051
LmnI GCTCC 5 cut(s) 47, 217, 236, 518, 656
Lsp1109I GCAGC 3 cut(s) 215, 424, 446
LweI GCATC 2 cut(s) 38, 707
MaeI CTAG 3 cut(s) 99, 375, 1277
MaeIII GTNAC 3 cut(s) 195, 367, 578
MalI GATC 5 cut(s) 6, 126, 142, 279, 1053
MbiI CCGCTC 1 cut(s) 513
MboI GATC 5 cut(s) 4, 124, 140, 277, 1051
MboII GAAGA 5 cut(s) 140, 827, 982, 1187, 1370
MfeI CAATTG 1 cut(s) 8
MhlI GDGCHC 4 cut(s) 98, 222, 660, 901
MlsI TGGCCA 1 cut(s) 119
MluNI TGGCCA 1 cut(s) 119
MlyI GAGTC 3 cut(s) 269, 382, 399
MmeI TCCRAC 1 cut(s) 258
Mox20I TGGCCA 1 cut(s) 119
MroXI GAANNNNTTC 1 cut(s) 819
MscI TGGCCA 1 cut(s) 119
MseI TTAA 5 cut(s) 1068, 1082, 1152, 1265, 1370
MslI CAYNNNNRTG 1 cut(s) 362
Msp20I TGGCCA 1 cut(s) 119
MspA1I CMGCKG 4 cut(s) 70, 193, 228, 437
MspCI CTTAAG 1 cut(s) 1369
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 3 cut(s) 209, 479, 493
MunI CAATTG 1 cut(s) 8
MvaI CCWGG 3 cut(s) 209, 479, 493
MvnI CGCG 2 cut(s) 70, 255
MwoI GCNNNNNNNGC 6 cut(s) 434, 785, 901, 1106, 1237, 1286
NcoI CCATGG 1 cut(s) 363
NdeII GATC 5 cut(s) 4, 124, 140, 277, 1051
NlaIII CATG 5 cut(s) 109, 367, 720, 771, 1226
NlaIV GGNNCC 2 cut(s) 206, 219
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 2 cut(s) 195, 367
PdmI GAANNNNTTC 1 cut(s) 819
PfeI GAWTC 2 cut(s) 527, 962
PflFI GACNNNGTC 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 477
PkrI GCNGC 6 cut(s) 72, 75, 230, 436, 439, 743
PleI GAGTC 3 cut(s) 268, 382, 399
PpsI GAGTC 3 cut(s) 268, 382, 399
Psp124BI GAGCTC 2 cut(s) 98, 901
Psp6I CCWGG 3 cut(s) 207, 477, 491
PspGI CCWGG 3 cut(s) 207, 477, 491
PspN4I GGNNCC 2 cut(s) 206, 219
PspPI GGNCC 1 cut(s) 246
PstI CTGCAG 1 cut(s) 436
PsyI GACNNNGTC 1 cut(s) 391
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 1 cut(s) 721
RsaNI GTAC 1 cut(s) 720
RseI CAYNNNNRTG 1 cut(s) 362
SacI GAGCTC 2 cut(s) 98, 901
SacII CCGCGG 1 cut(s) 71
SaqAI TTAA 5 cut(s) 1068, 1082, 1152, 1265, 1370
SatI GCNGC 6 cut(s) 71, 74, 229, 435, 438, 742
Sau3AI GATC 5 cut(s) 4, 124, 140, 277, 1051
Sau96I GGNCC 1 cut(s) 246
SchI GAGTC 3 cut(s) 269, 382, 399
ScrFI CCNGG 3 cut(s) 209, 479, 493
SduI GDGCHC 4 cut(s) 98, 222, 660, 901
SfaNI GCATC 2 cut(s) 38, 707
SfcI CTRYAG 1 cut(s) 432
Sfr303I CCGCGG 1 cut(s) 71
SgrBI CCGCGG 1 cut(s) 71
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 3 cut(s) 346, 829, 1369
SmoI CTYRAG 3 cut(s) 346, 829, 1369
SspMI CTAG 3 cut(s) 99, 375, 1277
SstI GAGCTC 2 cut(s) 98, 901
StyD4I CCNGG 3 cut(s) 207, 477, 491
StyI CCWWGG 2 cut(s) 77, 363
TaaI ACNGT 2 cut(s) 547, 645
TaqI TCGA 2 cut(s) 381, 1019
TatI WGTACW 1 cut(s) 719
TauI GCSGC 3 cut(s) 73, 76, 744
TfiI GAWTC 2 cut(s) 527, 962
Tru1I TTAA 5 cut(s) 1068, 1082, 1152, 1265, 1370
Tru9I TTAA 5 cut(s) 1068, 1082, 1152, 1265, 1370
TseFI GTSAC 2 cut(s) 195, 367
TseI GCWGC 3 cut(s) 228, 434, 437
Tsp45I GTSAC 2 cut(s) 195, 367
TspDTI ATGAA 3 cut(s) 94, 812, 830
TspGWI ACGGA 5 cut(s) 214, 351, 381, 664, 994
Tth111I GACNNNGTC 1 cut(s) 391
Vha464I CTTAAG 1 cut(s) 1369
XapI RAATTY 5 cut(s) 603, 794, 1012, 1038, 1324
XmiI GTMKAC 1 cut(s) 556
XmnI GAANNNNTTC 1 cut(s) 819
XspI CTAG 3 cut(s) 99, 375, 1277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.