Rh5BG001500

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0005101
Physical Location & Seq
Reverse (-)
202465 .. 207101
4637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG001500.1

Sequence Viewer

Length: 1344 bp
ATGGATCAATTGATGGTGGAAAAGTTGGCATCGGGCACGGGTGAGGGCGCAGCAGCCCCTCATCCTCAGCGGCGGCCAAGGGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGTGGCCGGTGATCCCTTTCCTACCAGATCACCTCTTCCTAAACATCAACAGGGACAGGGTGTCTCAACTCCAAGCCCGTACCTTGCAGAAAGACAGAGGTCATCCTCCGTGAATAGGCCACGGAGGCACTTGGACTTGGAGCCATTCCGCTGCTCTGACGAGAACAGGCCCGCCCGCCCCGAGTCTCCTCTGCCACCAGATCAGGCAAGGAAACAGCGTCCTGTCCCTGTCCCTGTCCCTGTCCCTTTTAAAGAGAACGGAGGAGGAGGAGGAGGCAAACCCCAACAGCATCATCTTCCTAAAACTTGCCCTGGGAGAGTAAGCAATAATGCTTTTACTACACCCTCACGACCTGACACACCCATGGTGACTGCTAGTCTCGATAGGACAAGAATCAGACAGCGGTCTTCCAATTCCAATGTCAGTAGTGCCACAGCAGCTACTAAGCTCTTGCAATCTACCACTGAGGCCACCTCCAGTGACACTAATCACACTCAAATCACCAGAAGGTGTCTTCCTGATGTTCGCTCCTCCATGCCCGAGGCCGATATGTTGTTGCCGACTGTTTCCAGTAGACAGACGACCGAAAACAGGCCTAATGCAACTGTCAATGGCAGCGATTTTTCAAAATTTTCTGCTTCCCCTTGCTCCCGTTCTCTCAACTTGCCACTGTCAAGCTCCGAGCACTTACTCTTCCAGTCAATCAAAGGCAGTGACAAATTAGAATCTGCTTTCTCCAAGCCATATGCAAATGCAGTGAAGATGGGTGGCCTCTGTCTGCCCCCAGTCCCACCATGTGCTAGTGCAAAGCCAGGTTCAGAAATAAGGAAAGGAAAGAAAGTTTCTAGTCTCCGAGAAGATGCTCATTCCTTGAGATTGCTGCAAAACCGCTATTTGCAGTGGAGATATGCCAATGCAACAGCACAGGCTTCCATGCTAGCTCAGCAGAGAGAGACTGAGAGAACACATTATTCTCTTGCATTAAAGATAACAGAACTATATGATTCTGTGAAGAGAAAACGGATAGACCTTGGTGTTTTGCAAAGAACAAAGACTTTATCGACAATTCTTGAAGCTCAAGTAAGGCTATTGGCTTTAGATGGTTTTGATTGTTTTGATGACTTTACGCAGCATGTTATCTTGCCTATGCTTGATCAAATAATTTGTTCTTGTATAAGCATATTGTTATCTGCATCATTCATGACAATTTTGCCTTTTATTTTGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

447

Amino Acids

49.21

Weight (kDa)

9.85

Isoelectric Point (pI)

66.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 180 - 403 2.8e-25 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 688
AciI CCGC 7 cut(s) 70, 73, 262, 285, 289, 517, 1003
AclWI GGATC 2 cut(s) 12, 119
AcoI YGGCCR 2 cut(s) 74, 117
AcsI RAATTY 1 cut(s) 743
AdeI CACNNNGTG 1 cut(s) 911
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 3 cut(s) 119, 228, 705
AgsI TTSAA 2 cut(s) 741, 1187
AhdI GACNNNNNGTC 2 cut(s) 173, 489
AjnI CCWGG 2 cut(s) 424, 925
AluBI AGCT 6 cut(s) 96, 554, 562, 792, 1055, 1190
AluI AGCT 6 cut(s) 96, 554, 562, 792, 1055, 1190
Alw21I GWGCWC 2 cut(s) 98, 801
Alw26I GTCTC 6 cut(s) 114, 181, 303, 497, 968, 1061
AlwI GGATC 2 cut(s) 12, 119
Ama87I CYCGRG 2 cut(s) 293, 653
AoxI GGCC 8 cut(s) 74, 117, 230, 281, 582, 657, 707, 883
ApeKI GCWGC 7 cut(s) 50, 53, 264, 551, 729, 994, 1243
ApoI RAATTY 1 cut(s) 743
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 7 cut(s) 53, 94, 103, 134, 135, 493, 607
AsuNHI GCTAGC 1 cut(s) 1051
AvaI CYCGRG 2 cut(s) 293, 653
BaeGI GKGCMC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 513, 620
Bbv12I GWGCWC 2 cut(s) 98, 801
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 7 cut(s) 62, 65, 251, 563, 741, 981, 1255
BccI CCATC 3 cut(s) 7, 871, 1208
BciT130I CCWGG 2 cut(s) 426, 927
BclI TGATCA 1 cut(s) 1267
BcoDI GTCTC 6 cut(s) 114, 181, 303, 497, 968, 1061
BfaI CTAG 5 cut(s) 99, 489, 915, 960, 1052
BglI GCCNNNNNGGC 1 cut(s) 238
BisI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 552, 730, 995, 1244
BlpI GCTNAGC 1 cut(s) 1056
BlsI GCNGC 9 cut(s) 52, 55, 72, 75, 266, 553, 731, 996, 1245
Bme1390I CCNGG 2 cut(s) 426, 927
BmeRI GACNNNNNGTC 2 cut(s) 173, 489
BmeT110I CYCGRG 2 cut(s) 293, 653
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 2 cut(s) 426, 927
BmrI ACTGGG 1 cut(s) 893
BmsI GCATC 4 cut(s) 38, 412, 964, 1316
BmtI GCTAGC 1 cut(s) 1055
BmuI ACTGGG 1 cut(s) 893
BoxI GACNNNNGTC 2 cut(s) 211, 517
BpiI GAAGAC 2 cut(s) 513, 620
BplI GAGNNNNNCTC 4 cut(s) 80, 112, 572, 604
BpmI CTGGAG 1 cut(s) 574
Bpu10I CCTNAGC 1 cut(s) 66
Bpu1102I GCTNAGC 1 cut(s) 1056
BpuEI CTTGAG 2 cut(s) 1006, 1176
BsaJI CCNNGG 7 cut(s) 77, 233, 424, 425, 477, 654, 1144
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 228, 705
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 4 cut(s) 591, 684, 811, 899
BseBI CCWGG 2 cut(s) 426, 927
BseDI CCNNGG 7 cut(s) 77, 233, 424, 425, 477, 654, 1144
BseGI GGATG 2 cut(s) 61, 215
BseLI CCNNNNNNNGG 3 cut(s) 119, 228, 705
BseMII CTCAG 4 cut(s) 80, 570, 1062, 1070
BseNI ACTGG 4 cut(s) 591, 684, 811, 899
BseRI GAGGAG 6 cut(s) 291, 390, 393, 396, 399, 634
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 7 cut(s) 62, 65, 251, 563, 741, 981, 1255
Bsh1285I CGRYCG 1 cut(s) 699
BshFI GGCC 8 cut(s) 76, 119, 232, 283, 584, 659, 709, 885
BsiEI CGRYCG 1 cut(s) 699
BsiHKAI GWGCWC 2 cut(s) 98, 801
BsiHKCI CYCGRG 2 cut(s) 293, 653
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 6 cut(s) 180, 323, 329, 335, 341, 887
BslI CCNNNNNNNGG 3 cut(s) 119, 228, 705
BsmAI GTCTC 6 cut(s) 114, 181, 303, 497, 968, 1061
BsmFI GGGAC 6 cut(s) 180, 323, 329, 335, 341, 887
BsnI GGCC 8 cut(s) 76, 119, 232, 283, 584, 659, 709, 885
BsoBI CYCGRG 2 cut(s) 293, 653
Bsp1286I GDGCHC 3 cut(s) 38, 98, 801
Bsp143I GATC 5 cut(s) 4, 124, 140, 313, 1267
Bsp1720I GCTNAGC 1 cut(s) 1056
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 7 cut(s) 70, 73, 262, 285, 289, 517, 1003
BspANI GGCC 8 cut(s) 76, 119, 232, 283, 584, 659, 709, 885
BspCNI CTCAG 4 cut(s) 79, 571, 1063, 1069
BspHI TCATGA 1 cut(s) 1314
BspLI GGNNCC 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 1055
BspPI GGATC 2 cut(s) 12, 119
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 4 cut(s) 591, 684, 811, 899
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 7 cut(s) 77, 233, 424, 425, 477, 654, 1144
BssMI GATC 5 cut(s) 4, 124, 140, 313, 1267
BssT1I CCWWGG 3 cut(s) 77, 477, 1144
Bst2UI CCWGG 2 cut(s) 426, 927
Bst4CI ACNGT 3 cut(s) 679, 721, 786
Bst6I CTCTTC 3 cut(s) 153, 812, 1121
BstC8I GCNNGC 3 cut(s) 285, 289, 1053
BstDEI CTNAG 5 cut(s) 66, 558, 579, 1056, 1071
BstDSI CCRYGG 2 cut(s) 233, 477
BstF5I GGATG 2 cut(s) 61, 215
BstHHI GCGC 1 cut(s) 50
BstKTI GATC 5 cut(s) 7, 127, 143, 316, 1270
BstMAI GTCTC 6 cut(s) 114, 181, 303, 497, 968, 1061
BstMBI GATC 5 cut(s) 4, 124, 140, 313, 1267
BstMCI CGRYCG 1 cut(s) 699
BstMWI GCNNNNNNNGC 3 cut(s) 238, 551, 1057
BstNI CCWGG 2 cut(s) 426, 927
BstNSI RCATGY 1 cut(s) 1250
BstPAI GACNNNNGTC 2 cut(s) 211, 517
BstSCI CCNGG 2 cut(s) 424, 925
BstSLI GKGCMC 1 cut(s) 38
BstV1I GCAGC 7 cut(s) 62, 65, 251, 563, 741, 981, 1255
BstV2I GAAGAC 2 cut(s) 513, 620
BsuRI GGCC 8 cut(s) 76, 119, 232, 283, 584, 659, 709, 885
BtgI CCRYGG 2 cut(s) 233, 477
BtsCI GGATG 2 cut(s) 61, 215
BtsI GCAGTG 3 cut(s) 832, 876, 1019
BtsIMutI CAGTG 6 cut(s) 576, 598, 782, 832, 876, 1019
Cac8I GCNNGC 3 cut(s) 285, 289, 1053
CciI TCATGA 1 cut(s) 1314
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 282
CseI GACGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 193
CviAII CATG 7 cut(s) 106, 478, 649, 909, 1048, 1247, 1315
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 5 cut(s) 66, 558, 579, 1056, 1071
DpnI GATC 5 cut(s) 6, 126, 142, 315, 1269
DpnII GATC 5 cut(s) 4, 124, 140, 313, 1267
DraI TTTAAA 1 cut(s) 364
DraIII CACNNNGTG 1 cut(s) 911
DriI GACNNNNNGTC 2 cut(s) 173, 489
EaeI YGGCCR 2 cut(s) 74, 117
Eam1104I CTCTTC 3 cut(s) 153, 812, 1121
Eam1105I GACNNNNNGTC 2 cut(s) 173, 489
EarI CTCTTC 3 cut(s) 153, 812, 1121
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 3 cut(s) 77, 477, 1144
Eco147I AGGCCT 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 293, 653
EcoICRI GAGCTC 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 424, 925
EcoT14I CCWWGG 3 cut(s) 77, 477, 1144
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 3 cut(s) 77, 477, 1144
FaeI CATG 7 cut(s) 109, 481, 652, 912, 1051, 1250, 1318
FaqI GGGAC 6 cut(s) 180, 323, 329, 335, 341, 887
FatI CATG 7 cut(s) 105, 477, 648, 908, 1047, 1246, 1314
FauI CCCGC 2 cut(s) 292, 296
FauNDI CATATG 1 cut(s) 859
FbaI TGATCA 1 cut(s) 1267
FblI GTMKAC 1 cut(s) 688
Fnu4HI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 552, 730, 995, 1244
FokI GGATG 2 cut(s) 48, 202
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 552, 730, 995, 1244
FspBI CTAG 5 cut(s) 99, 489, 915, 960, 1052
GlaI GCGC 1 cut(s) 49
GluI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 552, 730, 995, 1244
GsuI CTGGAG 1 cut(s) 574
HaeIII GGCC 8 cut(s) 76, 119, 232, 283, 584, 659, 709, 885
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 320
HhaI GCGC 1 cut(s) 50
Hin1II CATG 7 cut(s) 109, 481, 652, 912, 1051, 1250, 1318
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HinfI GANTC 4 cut(s) 296, 507, 839, 1118
HpaII CCGG 1 cut(s) 120
HphI GGTGA 7 cut(s) 53, 94, 103, 134, 135, 493, 607
Hpy166II GTNNAC 1 cut(s) 689
Hpy188I TCNGA 5 cut(s) 271, 512, 796, 934, 968
Hpy188III TCNNGA 5 cut(s) 462, 494, 632, 1184, 1315
Hpy8I GTNNAC 1 cut(s) 689
HpyAV CCTTC 1 cut(s) 615
HpyCH4III ACNGT 3 cut(s) 679, 721, 786
HpyF10VI GCNNNNNNNGC 3 cut(s) 238, 551, 1057
HpyF3I CTNAG 5 cut(s) 66, 558, 579, 1056, 1071
Hsp92II CATG 7 cut(s) 109, 481, 652, 912, 1051, 1250, 1318
HspAI GCGC 1 cut(s) 48
Ksp22I TGATCA 1 cut(s) 1267
Kzo9I GATC 5 cut(s) 4, 124, 140, 313, 1267
LmnI GCTCC 4 cut(s) 253, 647, 767, 797
Lsp1109I GCAGC 7 cut(s) 62, 65, 251, 563, 741, 981, 1255
LweI GCATC 4 cut(s) 38, 412, 964, 1316
MaeI CTAG 5 cut(s) 99, 489, 915, 960, 1052
MaeIII GTNAC 3 cut(s) 481, 593, 827
MalI GATC 5 cut(s) 6, 126, 142, 315, 1269
MboI GATC 5 cut(s) 4, 124, 140, 313, 1267
MboII GAAGA 8 cut(s) 140, 401, 513, 620, 799, 886, 983, 1138
MfeI CAATTG 1 cut(s) 8
MhlI GDGCHC 3 cut(s) 38, 98, 801
MluCI AATT 7 cut(s) 8, 526, 743, 833, 1179, 1275, 1320
MlyI GAGTC 1 cut(s) 305
MseI TTAA 2 cut(s) 363, 1097
MslI CAYNNNNRTG 1 cut(s) 476
MspA1I CMGCKG 3 cut(s) 70, 264, 517
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 426, 927
MunI CAATTG 1 cut(s) 8
MvaI CCWGG 2 cut(s) 426, 927
MwoI GCNNNNNNNGC 3 cut(s) 238, 551, 1057
NcoI CCATGG 1 cut(s) 477
NdeI CATATG 1 cut(s) 859
NdeII GATC 5 cut(s) 4, 124, 140, 313, 1267
NheI GCTAGC 1 cut(s) 1051
NlaIII CATG 7 cut(s) 109, 481, 652, 912, 1051, 1250, 1318
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 3 cut(s) 481, 593, 827
NspI RCATGY 1 cut(s) 1250
PagI TCATGA 1 cut(s) 1314
PasI CCCWGGG 1 cut(s) 425
PceI AGGCCT 1 cut(s) 709
PfeI GAWTC 3 cut(s) 507, 839, 1118
PkrI GCNGC 9 cut(s) 52, 55, 72, 75, 266, 553, 731, 996, 1245
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PshAI GACNNNNGTC 2 cut(s) 211, 517
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 424, 925
PspGI CCWGG 2 cut(s) 424, 925
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 282
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 476
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 2 cut(s) 363, 1097
SatI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 552, 730, 995, 1244
Sau3AI GATC 5 cut(s) 4, 124, 140, 313, 1267
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 426, 927
SduI GDGCHC 3 cut(s) 38, 98, 801
SfaNI GCATC 4 cut(s) 38, 412, 964, 1316
SmiMI CAYNNNNRTG 1 cut(s) 476
SmlI CTYRAG 2 cut(s) 985, 1191
SmoI CTYRAG 2 cut(s) 985, 1191
Sse9I AATT 7 cut(s) 8, 526, 743, 833, 1179, 1275, 1320
SseBI AGGCCT 1 cut(s) 709
SsiI CCGC 7 cut(s) 70, 73, 262, 285, 289, 517, 1003
SspMI CTAG 5 cut(s) 99, 489, 915, 960, 1052
SstI GAGCTC 1 cut(s) 98
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 2 cut(s) 424, 925
StyI CCWWGG 3 cut(s) 77, 477, 1144
TaaI ACNGT 3 cut(s) 679, 721, 786
TaqI TCGA 2 cut(s) 495, 1175
TaqII GACCGA 1 cut(s) 713
TasI AATT 7 cut(s) 8, 526, 743, 833, 1179, 1275, 1320
TauI GCSGC 2 cut(s) 73, 76
TfiI GAWTC 3 cut(s) 507, 839, 1118
Tru1I TTAA 2 cut(s) 363, 1097
Tru9I TTAA 2 cut(s) 363, 1097
TscAI CASTG 6 cut(s) 583, 598, 789, 832, 876, 1019
TseFI GTSAC 3 cut(s) 481, 593, 827
TseI GCWGC 7 cut(s) 50, 53, 264, 551, 729, 994, 1243
Tsp45I GTSAC 3 cut(s) 481, 593, 827
TspDTI ATGAA 2 cut(s) 94, 1303
TspGWI ACGGA 4 cut(s) 211, 250, 387, 1150
TspRI CASTG 6 cut(s) 583, 598, 789, 832, 876, 1019
XapI RAATTY 1 cut(s) 743
XceI RCATGY 1 cut(s) 1250
XmiI GTMKAC 1 cut(s) 688
XspI CTAG 5 cut(s) 99, 489, 915, 960, 1052
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.