Rh5CG000300

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0005101
Physical Location & Seq
Reverse (-)
63408 .. 68036
4629 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG000300.1

Sequence Viewer

Length: 1557 bp
ATGGATCAATTGATGGTGGAAAAGTTGGCATTGGGCACGGGTGAGGGCGCAGCAGCCCCTCATCCTCAGCGGCGGCCAAGGGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGTGGCGGGTGATCCCTTTCCTACCAGATCACCTCTTCCTAAACATCAACAGGGACAGGGTGTCTCAACTCCAAGCCCGTACCTTGCAGAAAGACAGAGGTCATCCTCCGTGAATAGGCCACGGAGGCACTTGGACTTGGAGCCATTCCGCTGCTCTGACGAGAACAGGCCCGCCCGCCCCGAGTCTCCTCTGCCACCAGATCAGGCAAGGAAACAGCGTCCTGTCCCTGTCCCTGTCCCTTTTAAAGAGAACGGAGGAGGCAAACCCCAACTTCATCATCTTCCTAAAACATGCCCTGGGAAAGTAAGCAATAATGCTTTTACTACACCCTCACGACCTGACACACCCATGGTGACTGCTAGTCTCGATAGGACAAGAATCAGACAGCGGTCTTCCAATTCCAATGTCAGTAGTGCCACAGCAGCTACTAAGCTCTTGCAATCTACCACTGAGGCCACCTCCAGTGACACTAATCACACTCAAATCACCAGAAGGTGTCTTCCTGATGTTCGCTCCTCCATGCCCGAGGCTGATATGTTGTTGCCGACTGTTTCCAGTAGACCGGCGACCGAAAAAAGTTGTAACAGGCCTAATGCAACTGTCAATGGCAGCGATTTTTCAAAATTTTCTGCTTCCCCTTGCTCCCGTTCTCTCAATTTGCCACTGTCAAGCTCCGAGCACTTACTCTTCCAGTCAATCAAAGGCAGTGACAAATTAGAATCTGCTTTCTCCAAGCCATATGCAAATGCAGTGAAGATGGCCGGCCTCTGTCTGCCCCCAGTCCCACCATGTGCTAGTGCAAAGCCAGGTTCAGAAATAAGGAAAGGAAAGAAAGTTTCTAGTCTCCGAGAAGATGCTCATTCCTTGAGATTGCTGCAAAACCGCTATTTGCAGTGGAGATATGCCAATGCAACAGCACAGGCTTCCATGCTAGCTCAGCAGAGAGAGACTGAGAGAACACATTATTCTCTTGCATTAAAGATAACAGAACTATATGATTCTGTGAAGAGAAAACGGATAGACCTTGGCGTTTTGCAAAGAACAAAGACTTTATCGACAATTCTTGAAGCTCAAATTCCATATTTGGATCAGTGGTTTTCTCTTCAAGAGGATTATTCAATTTCTCTGGCAGAAGCAACTCAAGCTTTGTTGAATGCATCACTCCGGCTTCCAATCAGCGCCAATGTTAAGGCTAATAAACAAGAGGTAGAAGAGGCACTGAACTCAGCAATGGAAGTGATGGAGAAAATAGTTTTCCATGTTCAGCAATCTATGCCGAAGGCAGAGGAAACAGACCATTTGATTTCGGATCTAGCAAGAGTGATTGGTGGAGAAAGAGCTTTCGTTGAAGAATGTGGAAATCTGTTGTCCAAGACATATACAACACAGGTGGAAGAGTGTAGCGTAAGGGGTCATATTATCCAGTTGAAACAACACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

518

Amino Acids

57.3

Weight (kDa)

9.47

Isoelectric Point (pI)

64.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 175 - 487 1.7e-51 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 673
AciI CCGC 8 cut(s) 70, 73, 119, 262, 285, 289, 502, 997
AclWI GGATC 4 cut(s) 12, 119, 1209, 1431
AcoI YGGCCR 2 cut(s) 74, 873
AcsI RAATTY 2 cut(s) 737, 1188
AdeI CACNNNGTG 1 cut(s) 905
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 2 cut(s) 119, 228
AgsI TTSAA 7 cut(s) 735, 1181, 1220, 1233, 1267, 1463, 1543
AhdI GACNNNNNGTC 2 cut(s) 173, 474
AjnI CCWGG 2 cut(s) 409, 919
AluBI AGCT 8 cut(s) 96, 539, 547, 786, 1049, 1184, 1259, 1454
AluI AGCT 8 cut(s) 96, 539, 547, 786, 1049, 1184, 1259, 1454
Alw21I GWGCWC 2 cut(s) 98, 795
Alw26I GTCTC 6 cut(s) 114, 181, 303, 482, 962, 1055
AlwI GGATC 4 cut(s) 12, 119, 1209, 1431
Ama87I CYCGRG 2 cut(s) 293, 638
AoxI GGCC 7 cut(s) 74, 230, 281, 567, 701, 873, 877
ApeKI GCWGC 6 cut(s) 50, 53, 264, 536, 723, 988
ApoI RAATTY 2 cut(s) 737, 1188
AspLEI GCGC 2 cut(s) 50, 1295
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 7 cut(s) 53, 94, 103, 134, 135, 478, 592
AsuNHI GCTAGC 1 cut(s) 1045
AvaI CYCGRG 2 cut(s) 293, 638
BaeGI GKGCMC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 98
BbsI GAAGAC 2 cut(s) 498, 605
Bbv12I GWGCWC 2 cut(s) 98, 795
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 6 cut(s) 62, 65, 251, 548, 735, 975
BccI CCATC 3 cut(s) 7, 865, 1348
BciT130I CCWGG 2 cut(s) 411, 921
BcoDI GTCTC 6 cut(s) 114, 181, 303, 482, 962, 1055
BfaI CTAG 6 cut(s) 99, 474, 909, 954, 1046, 1427
BfoI RGCGCY 1 cut(s) 1296
BglI GCCNNNNNGGC 1 cut(s) 238
BisI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 537, 724, 989
BlpI GCTNAGC 1 cut(s) 1050
BlsI GCNGC 8 cut(s) 52, 55, 72, 75, 266, 538, 725, 990
Bme1390I CCNGG 2 cut(s) 411, 921
BmeRI GACNNNNNGTC 2 cut(s) 173, 474
BmeT110I CYCGRG 2 cut(s) 293, 638
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 2 cut(s) 411, 921
BmrI ACTGGG 1 cut(s) 887
BmsI GCATC 2 cut(s) 958, 1280
BmtI GCTAGC 1 cut(s) 1049
BmuI ACTGGG 1 cut(s) 887
BoxI GACNNNNGTC 2 cut(s) 211, 502
BpiI GAAGAC 2 cut(s) 498, 605
BplI GAGNNNNNCTC 4 cut(s) 80, 112, 557, 589
BpmI CTGGAG 1 cut(s) 559
Bpu10I CCTNAGC 1 cut(s) 66
Bpu1102I GCTNAGC 1 cut(s) 1050
BpuEI CTTGAG 2 cut(s) 1000, 1239
BsaJI CCNNGG 7 cut(s) 77, 233, 409, 410, 462, 639, 1138
Bsc4I CCNNNNNNNGG 2 cut(s) 119, 228
Bse118I RCCGGY 2 cut(s) 676, 875
Bse1I ACTGG 5 cut(s) 576, 669, 805, 893, 1537
Bse3DI GCAATG 1 cut(s) 1350
BseBI CCWGG 2 cut(s) 411, 921
BseDI CCNNGG 7 cut(s) 77, 233, 409, 410, 462, 639, 1138
BseGI GGATG 2 cut(s) 61, 215
BseLI CCNNNNNNNGG 2 cut(s) 119, 228
BseMI GCAATG 1 cut(s) 1350
BseMII CTCAG 5 cut(s) 80, 555, 1056, 1064, 1353
BseNI ACTGG 5 cut(s) 576, 669, 805, 893, 1537
BseRI GAGGAG 3 cut(s) 291, 384, 619
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 6 cut(s) 62, 65, 251, 548, 735, 975
Bsh1285I CGRYCG 1 cut(s) 684
BshFI GGCC 7 cut(s) 76, 232, 283, 569, 703, 875, 879
BsiEI CGRYCG 1 cut(s) 684
BsiHKAI GWGCWC 2 cut(s) 98, 795
BsiHKCI CYCGRG 2 cut(s) 293, 638
BsiSI CCGG 3 cut(s) 677, 876, 1279
BslFI GGGAC 5 cut(s) 180, 323, 329, 335, 881
BslI CCNNNNNNNGG 2 cut(s) 119, 228
BsmAI GTCTC 6 cut(s) 114, 181, 303, 482, 962, 1055
BsmFI GGGAC 5 cut(s) 180, 323, 329, 335, 881
BsmI GAATGC 1 cut(s) 1273
BsnI GGCC 7 cut(s) 76, 232, 283, 569, 703, 875, 879
BsoBI CYCGRG 2 cut(s) 293, 638
Bsp1286I GDGCHC 3 cut(s) 38, 98, 795
Bsp143I GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
Bsp1720I GCTNAGC 1 cut(s) 1050
Bsp19I CCATGG 1 cut(s) 462
BspACI CCGC 8 cut(s) 70, 73, 119, 262, 285, 289, 502, 997
BspANI GGCC 7 cut(s) 76, 232, 283, 569, 703, 875, 879
BspCNI CTCAG 5 cut(s) 79, 556, 1057, 1063, 1352
BspLI GGNNCC 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 1049
BspPI GGATC 4 cut(s) 12, 119, 1209, 1431
BsrDI GCAATG 1 cut(s) 1350
BsrFI RCCGGY 2 cut(s) 676, 875
BsrI ACTGG 5 cut(s) 576, 669, 805, 893, 1537
BssAI RCCGGY 2 cut(s) 676, 875
BssECI CCNNGG 7 cut(s) 77, 233, 409, 410, 462, 639, 1138
BssMI GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
BssT1I CCWWGG 3 cut(s) 77, 462, 1138
Bst2UI CCWGG 2 cut(s) 411, 921
Bst4CI ACNGT 4 cut(s) 664, 715, 780, 1553
Bst6I CTCTTC 6 cut(s) 153, 806, 1115, 1221, 1320, 1503
BstAPI GCANNNNNTGC 1 cut(s) 1387
BstC8I GCNNGC 4 cut(s) 285, 289, 877, 1047
BstDEI CTNAG 6 cut(s) 66, 543, 564, 1050, 1065, 1339
BstDSI CCRYGG 2 cut(s) 233, 462
BstF5I GGATG 2 cut(s) 61, 215
BstH2I RGCGCY 1 cut(s) 1296
BstHHI GCGC 2 cut(s) 50, 1295
BstKTI GATC 6 cut(s) 7, 127, 143, 316, 1204, 1426
BstMAI GTCTC 6 cut(s) 114, 181, 303, 482, 962, 1055
BstMBI GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
BstMCI CGRYCG 1 cut(s) 684
BstMWI GCNNNNNNNGC 5 cut(s) 238, 536, 1051, 1256, 1387
BstNI CCWGG 2 cut(s) 411, 921
BstNSI RCATGY 1 cut(s) 408
BstPAI GACNNNNGTC 2 cut(s) 211, 502
BstSCI CCNGG 2 cut(s) 409, 919
BstSLI GKGCMC 1 cut(s) 38
BstV1I GCAGC 6 cut(s) 62, 65, 251, 548, 735, 975
BstV2I GAAGAC 2 cut(s) 498, 605
BstX2I RGATCY 1 cut(s) 1423
BstYI RGATCY 1 cut(s) 1423
BsuRI GGCC 7 cut(s) 76, 232, 283, 569, 703, 875, 879
BtgI CCRYGG 2 cut(s) 233, 462
BtsCI GGATG 2 cut(s) 61, 215
BtsI GCAGTG 3 cut(s) 826, 870, 1013
BtsIMutI CAGTG 9 cut(s) 561, 583, 776, 826, 870, 1013, 1211, 1331, 1549
Cac8I GCNNGC 4 cut(s) 285, 289, 877, 1047
CfoI GCGC 2 cut(s) 50, 1295
Cfr10I RCCGGY 2 cut(s) 676, 875
Cfr13I GGNCC 1 cut(s) 282
CseI GACGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 193
CspCI CAANNNNNGTGG 2 cut(s) 1485, 1520
CviAII CATG 7 cut(s) 106, 405, 463, 634, 903, 1042, 1373
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 6 cut(s) 66, 543, 564, 1050, 1065, 1339
DpnI GATC 6 cut(s) 6, 126, 142, 315, 1203, 1425
DpnII GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
DraI TTTAAA 1 cut(s) 358
DraIII CACNNNGTG 1 cut(s) 905
DriI GACNNNNNGTC 2 cut(s) 173, 474
EaeI YGGCCR 2 cut(s) 74, 873
Eam1104I CTCTTC 6 cut(s) 153, 806, 1115, 1221, 1320, 1503
Eam1105I GACNNNNNGTC 2 cut(s) 173, 474
EarI CTCTTC 6 cut(s) 153, 806, 1115, 1221, 1320, 1503
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 3 cut(s) 77, 462, 1138
Eco147I AGGCCT 1 cut(s) 703
Eco24I GRGCYC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 293, 638
EcoICRI GAGCTC 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 409, 919
EcoT14I CCWWGG 3 cut(s) 77, 462, 1138
EcoT22I ATGCAT 1 cut(s) 1273
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 3 cut(s) 77, 462, 1138
FaeI CATG 7 cut(s) 109, 408, 466, 637, 906, 1045, 1376
FaqI GGGAC 5 cut(s) 180, 323, 329, 335, 881
FatI CATG 7 cut(s) 105, 404, 462, 633, 902, 1041, 1372
FauI CCCGC 3 cut(s) 112, 292, 296
FauNDI CATATG 1 cut(s) 853
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 537, 724, 989
FokI GGATG 2 cut(s) 48, 202
FriOI GRGCYC 1 cut(s) 98
FseI GGCCGGCC 1 cut(s) 879
Fsp4HI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 537, 724, 989
FspBI CTAG 6 cut(s) 99, 474, 909, 954, 1046, 1427
GlaI GCGC 2 cut(s) 49, 1294
GluI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 537, 724, 989
GsuI CTGGAG 1 cut(s) 559
HaeII RGCGCY 1 cut(s) 1296
HaeIII GGCC 7 cut(s) 76, 232, 283, 569, 703, 875, 879
HapII CCGG 3 cut(s) 677, 876, 1279
HgaI GACGC 1 cut(s) 320
HhaI GCGC 2 cut(s) 50, 1295
Hin1II CATG 7 cut(s) 109, 408, 466, 637, 906, 1045, 1376
Hin6I GCGC 2 cut(s) 48, 1293
HinP1I GCGC 2 cut(s) 48, 1293
HindIII AAGCTT 1 cut(s) 1257
HinfI GANTC 4 cut(s) 296, 492, 833, 1112
HpaII CCGG 3 cut(s) 677, 876, 1279
HphI GGTGA 7 cut(s) 53, 94, 103, 134, 135, 478, 592
Hpy166II GTNNAC 1 cut(s) 674
Hpy188I TCNGA 6 cut(s) 271, 497, 790, 928, 962, 1423
Hpy188III TCNNGA 5 cut(s) 447, 479, 617, 1178, 1220
Hpy8I GTNNAC 1 cut(s) 674
HpyAV CCTTC 2 cut(s) 600, 1387
HpyCH4III ACNGT 4 cut(s) 664, 715, 780, 1553
HpyF10VI GCNNNNNNNGC 5 cut(s) 238, 536, 1051, 1256, 1387
HpyF3I CTNAG 6 cut(s) 66, 543, 564, 1050, 1065, 1339
Hsp92II CATG 7 cut(s) 109, 408, 466, 637, 906, 1045, 1376
HspAI GCGC 2 cut(s) 48, 1293
KroI GCCGGC 1 cut(s) 875
KroNI GCCGGC 1 cut(s) 877
Kzo9I GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
LmnI GCTCC 4 cut(s) 253, 632, 761, 791
Lsp1109I GCAGC 6 cut(s) 62, 65, 251, 548, 735, 975
LweI GCATC 2 cut(s) 958, 1280
MaeI CTAG 6 cut(s) 99, 474, 909, 954, 1046, 1427
MaeIII GTNAC 4 cut(s) 466, 578, 695, 821
MalI GATC 6 cut(s) 6, 126, 142, 315, 1203, 1425
MboI GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 1 cut(s) 1423
MhlI GDGCHC 3 cut(s) 38, 98, 795
MluCI AATT 8 cut(s) 8, 511, 737, 769, 827, 1173, 1188, 1233
MlyI GAGTC 1 cut(s) 305
Mph1103I ATGCAT 1 cut(s) 1273
MroNI GCCGGC 1 cut(s) 875
MseI TTAA 3 cut(s) 357, 1091, 1302
MslI CAYNNNNRTG 1 cut(s) 461
MspA1I CMGCKG 3 cut(s) 70, 264, 502
MspI CCGG 3 cut(s) 677, 876, 1279
MspR9I CCNGG 2 cut(s) 411, 921
MunI CAATTG 1 cut(s) 8
Mva1269I GAATGC 1 cut(s) 1273
MvaI CCWGG 2 cut(s) 411, 921
MwoI GCNNNNNNNGC 5 cut(s) 238, 536, 1051, 1256, 1387
NaeI GCCGGC 1 cut(s) 877
NcoI CCATGG 1 cut(s) 462
NdeI CATATG 1 cut(s) 853
NdeII GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
NgoMIV GCCGGC 1 cut(s) 875
NheI GCTAGC 1 cut(s) 1045
NlaIII CATG 7 cut(s) 109, 408, 466, 637, 906, 1045, 1376
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 3 cut(s) 466, 578, 821
NsiI ATGCAT 1 cut(s) 1273
NspI RCATGY 1 cut(s) 408
PasI CCCWGGG 1 cut(s) 410
PceI AGGCCT 1 cut(s) 703
PctI GAATGC 1 cut(s) 1273
PdiI GCCGGC 1 cut(s) 877
PfeI GAWTC 3 cut(s) 492, 833, 1112
PkrI GCNGC 8 cut(s) 52, 55, 72, 75, 266, 538, 725, 990
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PshAI GACNNNNGTC 2 cut(s) 211, 502
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 409, 919
PspGI CCWGG 2 cut(s) 409, 919
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 1423
RigI GGCCGGCC 1 cut(s) 879
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 461
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 3 cut(s) 357, 1091, 1302
SatI GCNGC 8 cut(s) 51, 54, 71, 74, 265, 537, 724, 989
Sau3AI GATC 6 cut(s) 4, 124, 140, 313, 1201, 1423
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 411, 921
SduI GDGCHC 3 cut(s) 38, 98, 795
SfaNI GCATC 2 cut(s) 958, 1280
SmiMI CAYNNNNRTG 1 cut(s) 461
SmlI CTYRAG 2 cut(s) 979, 1254
SmoI CTYRAG 2 cut(s) 979, 1254
Sse9I AATT 8 cut(s) 8, 511, 737, 769, 827, 1173, 1188, 1233
SseBI AGGCCT 1 cut(s) 703
SsiI CCGC 8 cut(s) 70, 73, 119, 262, 285, 289, 502, 997
SspMI CTAG 6 cut(s) 99, 474, 909, 954, 1046, 1427
SstI GAGCTC 1 cut(s) 98
StuI AGGCCT 1 cut(s) 703
StyD4I CCNGG 2 cut(s) 409, 919
StyI CCWWGG 3 cut(s) 77, 462, 1138
TaaI ACNGT 4 cut(s) 664, 715, 780, 1553
TaqI TCGA 2 cut(s) 480, 1169
TaqII GACCGA 1 cut(s) 698
TasI AATT 8 cut(s) 8, 511, 737, 769, 827, 1173, 1188, 1233
TauI GCSGC 2 cut(s) 73, 76
TfiI GAWTC 3 cut(s) 492, 833, 1112
Tru1I TTAA 3 cut(s) 357, 1091, 1302
Tru9I TTAA 3 cut(s) 357, 1091, 1302
TscAI CASTG 9 cut(s) 568, 583, 783, 826, 870, 1013, 1211, 1338, 1556
TseFI GTSAC 3 cut(s) 466, 578, 821
TseI GCWGC 6 cut(s) 50, 53, 264, 536, 723, 988
Tsp45I GTSAC 3 cut(s) 466, 578, 821
TspDTI ATGAA 2 cut(s) 94, 377
TspGWI ACGGA 4 cut(s) 211, 250, 381, 1144
TspRI CASTG 9 cut(s) 568, 583, 783, 826, 870, 1013, 1211, 1338, 1556
XapI RAATTY 2 cut(s) 737, 1188
XceI RCATGY 1 cut(s) 408
XmiI GTMKAC 1 cut(s) 673
XspI CTAG 6 cut(s) 99, 474, 909, 954, 1046, 1427
Zsp2I ATGCAT 1 cut(s) 1273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.