Rw5G000060

Protein ENDOSPERM DEFECTIVE 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0005101
Physical Location & Seq
Reverse (-)
117416 .. 121467
4052 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G000060.1

Sequence Viewer

Length: 1563 bp
ATGGATCAATTGATGGTGGAAAAGTTGGCATCGGGCACGGGTGAGGGCGCAGCAGCCCCTCATCCTCAGCGGCGGCCAAGGGTGAGGGAGGTGAGCTCTAGGTTCATGTCTCCTGTGGCCGGTGATCCCTTTCCTACCAGATCACCTCTTCCTAAACATCAACAGGGACAGGGTGTCTCAACTCCAAGCCCGTACCTTGCAGAAAGACAGAGGTCATCCTCCGTGAATAAGCCACGGAGGCACTTGGACTTGGAGCCATTCCGCTGCTCTGACGAGAACAGGCCCGCCCGCCCCGAGTCTCCTCTGCCACCAGATCAGGCAAGGAAACAGCGTCCTGTCCCTGTCCCTGTCCCTGTCCCTTTTAAAGAGAACGGAGGAGGCAAACCCCAACTGCATCATCTTCCTAAAACATGCCCTGGGAAAGTAAGCAATAATGCTTTTACTACACCCTCACGACCTGACACACCCTTGGTGACTGCTAGTCTCGATAGGACAAGAATCAGACAGCGTTCTTCCAATTCCAATGTCAGTAGTGCCACAGCAGCTACTAAGCTCTTGCAATCTACCACTGAGGCCACCTCCAGTGACACTAATCATACTCAAATTACCAGAAGGTGTCTTCCTGATGTTCGCTCCTCCATGCCCGAGGCCGATATGTTGTTGCCGACTGTTTCCAGTAGACAGGCGACCGAAAAAAGTTGTAACAGGCCTAATGCAACTGTCAATGGCAGCGATTTTTCAAAATTTTCTGCTTCCCCTTGCTCCCGTTCTCTCAACTTGCCACTGTCAAGCTCCGAGCACTTACTCTTCCAGTCAATCAAAGGCAGTGACAAATTAGAATCTGCTTTCTCCAAGCCATATGCAAATGCAGTGAAGATGGGCGGCCTCTCTCTGCCCCCAGTCCCACCATGTGCTAGTGCAAAGCCAGGTTCAGAAATAAGGAAAGGAAAGAAAGTTTCTAGTCTCCGAGAAGATGCTCATTCCTTGAGATTGCTGCAAAACCGCTATTTGCAGTGGAGATATGCCAATGCAACAGCACAGGCTTCCATGCTAGCTCAGCAGAGAGAGACTGAGAGAACACATTATTCTCTTGCATTAAAGATAACAGAACTATATGATTCTGTGAAGAGAAAACGGATAGACCTTGGCGTTTTGCAAAGAACAAAGACTTTATCGACAATTCTTGAAGCTCAAATTCCATATTTGGATCAGTGGTTTTCTCTTCAAGAGGATTATTCAATTTCTCTGGCAGAAGCAACTCAAGCTTTGTTGAATGCATCACTCCAGCTTCCAATCAGCGCCAATGTTAAGGCTAATAAACAAGAGGTAGAAGAGGCACTGAACTCAGCAATGGAAGTGATGGAGAAAATAGTTTTCCATGTTCAGCAATCTATGCCGAAGGCAGAGGAAACAGACCATTTGATTTCGGATCTAGCAAGAGTGATTGGTGGAGAAAGAGCTTTCGTTGAAGAATGTGGAAATCTGTTGTCCAAGACATATACAACACAGGTGGAAGAGTGTAGCTTAAGGGGTCATATTATCCAGTTGAAACAACACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
QWRF

Protein Analysis

520

Amino Acids

57.41

Weight (kDa)

9.44

Isoelectric Point (pI)

64.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
QWRF PF04484 177 - 489 1.7e-49 QWRF family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 679
AciI CCGC 7 cut(s) 70, 73, 262, 285, 289, 882, 1003
AclWI GGATC 4 cut(s) 12, 119, 1215, 1437
AcoI YGGCCR 2 cut(s) 74, 117
AcsI RAATTY 2 cut(s) 743, 1194
AdeI CACNNNGTG 1 cut(s) 911
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 119
AflII CTTAAG 1 cut(s) 1525
AgsI TTSAA 7 cut(s) 741, 1187, 1226, 1239, 1273, 1469, 1549
AhdI GACNNNNNGTC 2 cut(s) 173, 480
AjnI CCWGG 2 cut(s) 415, 925
Alw21I GWGCWC 2 cut(s) 98, 801
Alw26I GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
AlwI GGATC 4 cut(s) 12, 119, 1215, 1437
Ama87I CYCGRG 2 cut(s) 293, 644
AoxI GGCC 7 cut(s) 74, 117, 281, 573, 648, 707, 883
ApeKI GCWGC 6 cut(s) 50, 53, 264, 542, 729, 994
ApoI RAATTY 2 cut(s) 743, 1194
AspLEI GCGC 2 cut(s) 50, 1301
AspS9I GGNCC 1 cut(s) 282
AsuHPI GGTGA 6 cut(s) 53, 94, 103, 134, 135, 484
AsuNHI GCTAGC 1 cut(s) 1051
AvaI CYCGRG 2 cut(s) 293, 644
BaeGI GKGCMC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 98
BbsI GAAGAC 1 cut(s) 611
Bbv12I GWGCWC 2 cut(s) 98, 801
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 6 cut(s) 62, 65, 251, 554, 741, 981
BccI CCATC 3 cut(s) 7, 871, 1354
BciT130I CCWGG 2 cut(s) 417, 927
BcoDI GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
BfaI CTAG 6 cut(s) 99, 480, 915, 960, 1052, 1433
BfoI RGCGCY 1 cut(s) 1302
BfrI CTTAAG 1 cut(s) 1525
BglI GCCNNNNNGGC 1 cut(s) 238
BisI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 543, 730, 883, 995
BlpI GCTNAGC 1 cut(s) 1056
BlsI GCNGC 9 cut(s) 52, 55, 72, 75, 266, 544, 731, 884, 996
Bme1390I CCNGG 2 cut(s) 417, 927
BmeRI GACNNNNNGTC 2 cut(s) 173, 480
BmeT110I CYCGRG 2 cut(s) 293, 644
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 2 cut(s) 417, 927
BmrI ACTGGG 1 cut(s) 893
BmsI GCATC 4 cut(s) 38, 403, 964, 1286
BmtI GCTAGC 1 cut(s) 1055
BmuI ACTGGG 1 cut(s) 893
BoxI GACNNNNGTC 1 cut(s) 211
BpiI GAAGAC 1 cut(s) 611
BplI GAGNNNNNCTC 4 cut(s) 80, 112, 563, 595
BpmI CTGGAG 2 cut(s) 565, 1268
Bpu10I CCTNAGC 1 cut(s) 66
Bpu1102I GCTNAGC 1 cut(s) 1056
BpuEI CTTGAG 2 cut(s) 1006, 1245
BsaJI CCNNGG 7 cut(s) 77, 233, 415, 416, 468, 645, 1144
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 5 cut(s) 582, 675, 811, 899, 1543
Bse3DI GCAATG 1 cut(s) 1356
BseBI CCWGG 2 cut(s) 417, 927
BseDI CCNNGG 7 cut(s) 77, 233, 415, 416, 468, 645, 1144
BseGI GGATG 2 cut(s) 61, 215
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMI GCAATG 1 cut(s) 1356
BseMII CTCAG 5 cut(s) 80, 561, 1062, 1070, 1359
BseNI ACTGG 5 cut(s) 582, 675, 811, 899, 1543
BseRI GAGGAG 3 cut(s) 291, 390, 625
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 6 cut(s) 62, 65, 251, 554, 741, 981
Bsh1285I CGRYCG 1 cut(s) 690
BshFI GGCC 7 cut(s) 76, 119, 283, 575, 650, 709, 885
BsiEI CGRYCG 1 cut(s) 690
BsiHKAI GWGCWC 2 cut(s) 98, 801
BsiHKCI CYCGRG 2 cut(s) 293, 644
BsiSI CCGG 1 cut(s) 120
BslFI GGGAC 6 cut(s) 180, 323, 329, 335, 341, 887
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
BsmFI GGGAC 6 cut(s) 180, 323, 329, 335, 341, 887
BsmI GAATGC 1 cut(s) 1279
BsnI GGCC 7 cut(s) 76, 119, 283, 575, 650, 709, 885
BsoBI CYCGRG 2 cut(s) 293, 644
Bsp1286I GDGCHC 3 cut(s) 38, 98, 801
Bsp143I GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
Bsp1720I GCTNAGC 1 cut(s) 1056
BspACI CCGC 7 cut(s) 70, 73, 262, 285, 289, 882, 1003
BspANI GGCC 7 cut(s) 76, 119, 283, 575, 650, 709, 885
BspCNI CTCAG 5 cut(s) 79, 562, 1063, 1069, 1358
BspLI GGNNCC 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 1055
BspPI GGATC 4 cut(s) 12, 119, 1215, 1437
BspTI CTTAAG 1 cut(s) 1525
BsrDI GCAATG 1 cut(s) 1356
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 5 cut(s) 582, 675, 811, 899, 1543
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 7 cut(s) 77, 233, 415, 416, 468, 645, 1144
BssMI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
BssT1I CCWWGG 3 cut(s) 77, 468, 1144
Bst2UI CCWGG 2 cut(s) 417, 927
Bst4CI ACNGT 4 cut(s) 670, 721, 786, 1559
Bst6I CTCTTC 6 cut(s) 153, 812, 1121, 1227, 1326, 1509
BstAFI CTTAAG 1 cut(s) 1525
BstAPI GCANNNNNTGC 1 cut(s) 1393
BstC8I GCNNGC 3 cut(s) 285, 289, 1053
BstDEI CTNAG 6 cut(s) 66, 549, 570, 1056, 1071, 1345
BstDSI CCRYGG 1 cut(s) 233
BstF5I GGATG 2 cut(s) 61, 215
BstH2I RGCGCY 1 cut(s) 1302
BstHHI GCGC 2 cut(s) 50, 1301
BstKTI GATC 6 cut(s) 7, 127, 143, 316, 1210, 1432
BstMAI GTCTC 6 cut(s) 114, 181, 303, 488, 968, 1061
BstMBI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
BstMCI CGRYCG 1 cut(s) 690
BstMWI GCNNNNNNNGC 5 cut(s) 238, 542, 1057, 1262, 1393
BstNI CCWGG 2 cut(s) 417, 927
BstNSI RCATGY 1 cut(s) 414
BstPAI GACNNNNGTC 1 cut(s) 211
BstSCI CCNGG 2 cut(s) 415, 925
BstSLI GKGCMC 1 cut(s) 38
BstV1I GCAGC 6 cut(s) 62, 65, 251, 554, 741, 981
BstV2I GAAGAC 1 cut(s) 611
BstX2I RGATCY 1 cut(s) 1429
BstYI RGATCY 1 cut(s) 1429
BsuRI GGCC 7 cut(s) 76, 119, 283, 575, 650, 709, 885
BtgI CCRYGG 1 cut(s) 233
BtsCI GGATG 2 cut(s) 61, 215
BtsI GCAGTG 3 cut(s) 832, 876, 1019
BtsIMutI CAGTG 9 cut(s) 567, 589, 782, 832, 876, 1019, 1217, 1337, 1555
Cac8I GCNNGC 3 cut(s) 285, 289, 1053
CfoI GCGC 2 cut(s) 50, 1301
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 282
CseI GACGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 193
CspCI CAANNNNNGTGG 2 cut(s) 1491, 1526
CviAII CATG 6 cut(s) 106, 411, 640, 909, 1048, 1379
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 6 cut(s) 66, 549, 570, 1056, 1071, 1345
DpnI GATC 6 cut(s) 6, 126, 142, 315, 1209, 1431
DpnII GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
DraI TTTAAA 1 cut(s) 364
DraIII CACNNNGTG 1 cut(s) 911
DriI GACNNNNNGTC 2 cut(s) 173, 480
EaeI YGGCCR 2 cut(s) 74, 117
Eam1104I CTCTTC 6 cut(s) 153, 812, 1121, 1227, 1326, 1509
Eam1105I GACNNNNNGTC 2 cut(s) 173, 480
EarI CTCTTC 6 cut(s) 153, 812, 1121, 1227, 1326, 1509
Ecl136II GAGCTC 1 cut(s) 96
Eco130I CCWWGG 3 cut(s) 77, 468, 1144
Eco147I AGGCCT 1 cut(s) 709
Eco24I GRGCYC 1 cut(s) 98
Eco53kI GAGCTC 1 cut(s) 96
Eco88I CYCGRG 2 cut(s) 293, 644
EcoICRI GAGCTC 1 cut(s) 96
EcoRII CCWGG 2 cut(s) 415, 925
EcoT14I CCWWGG 3 cut(s) 77, 468, 1144
EcoT22I ATGCAT 1 cut(s) 1279
EcoT38I GRGCYC 1 cut(s) 98
ErhI CCWWGG 3 cut(s) 77, 468, 1144
FaeI CATG 6 cut(s) 109, 414, 643, 912, 1051, 1382
FaqI GGGAC 6 cut(s) 180, 323, 329, 335, 341, 887
FatI CATG 6 cut(s) 105, 410, 639, 908, 1047, 1378
FauI CCCGC 2 cut(s) 292, 296
FauNDI CATATG 1 cut(s) 859
FblI GTMKAC 1 cut(s) 679
Fnu4HI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 543, 730, 883, 995
FokI GGATG 2 cut(s) 48, 202
FriOI GRGCYC 1 cut(s) 98
Fsp4HI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 543, 730, 883, 995
FspBI CTAG 6 cut(s) 99, 480, 915, 960, 1052, 1433
GlaI GCGC 2 cut(s) 49, 1300
GluI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 543, 730, 883, 995
GsuI CTGGAG 2 cut(s) 565, 1268
HaeII RGCGCY 1 cut(s) 1302
HaeIII GGCC 7 cut(s) 76, 119, 283, 575, 650, 709, 885
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 320
HhaI GCGC 2 cut(s) 50, 1301
Hin1II CATG 6 cut(s) 109, 414, 643, 912, 1051, 1382
Hin6I GCGC 2 cut(s) 48, 1299
HinP1I GCGC 2 cut(s) 48, 1299
HindIII AAGCTT 1 cut(s) 1263
HinfI GANTC 4 cut(s) 296, 498, 839, 1118
HpaII CCGG 1 cut(s) 120
HphI GGTGA 6 cut(s) 53, 94, 103, 134, 135, 484
Hpy166II GTNNAC 1 cut(s) 680
Hpy188I TCNGA 6 cut(s) 271, 503, 796, 934, 968, 1429
Hpy188III TCNNGA 5 cut(s) 453, 485, 623, 1184, 1226
Hpy8I GTNNAC 1 cut(s) 680
HpyAV CCTTC 2 cut(s) 606, 1393
HpyCH4III ACNGT 4 cut(s) 670, 721, 786, 1559
HpyF10VI GCNNNNNNNGC 5 cut(s) 238, 542, 1057, 1262, 1393
HpyF3I CTNAG 6 cut(s) 66, 549, 570, 1056, 1071, 1345
Hsp92II CATG 6 cut(s) 109, 414, 643, 912, 1051, 1382
HspAI GCGC 2 cut(s) 48, 1299
Kzo9I GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
LmnI GCTCC 4 cut(s) 253, 638, 767, 797
Lsp1109I GCAGC 6 cut(s) 62, 65, 251, 554, 741, 981
LweI GCATC 4 cut(s) 38, 403, 964, 1286
MaeI CTAG 6 cut(s) 99, 480, 915, 960, 1052, 1433
MaeIII GTNAC 4 cut(s) 472, 584, 701, 827
MalI GATC 6 cut(s) 6, 126, 142, 315, 1209, 1431
MboI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
MfeI CAATTG 1 cut(s) 8
MflI RGATCY 1 cut(s) 1429
MhlI GDGCHC 3 cut(s) 38, 98, 801
MluCI AATT 8 cut(s) 8, 517, 603, 743, 833, 1179, 1194, 1239
MlyI GAGTC 1 cut(s) 305
Mph1103I ATGCAT 1 cut(s) 1279
MseI TTAA 4 cut(s) 363, 1097, 1308, 1526
MspA1I CMGCKG 2 cut(s) 70, 264
MspCI CTTAAG 1 cut(s) 1525
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 2 cut(s) 417, 927
MunI CAATTG 1 cut(s) 8
Mva1269I GAATGC 1 cut(s) 1279
MvaI CCWGG 2 cut(s) 417, 927
MwoI GCNNNNNNNGC 5 cut(s) 238, 542, 1057, 1262, 1393
NdeI CATATG 1 cut(s) 859
NdeII GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
NheI GCTAGC 1 cut(s) 1051
NlaIII CATG 6 cut(s) 109, 414, 643, 912, 1051, 1382
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 3 cut(s) 472, 584, 827
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 1 cut(s) 414
PasI CCCWGGG 1 cut(s) 416
PceI AGGCCT 1 cut(s) 709
PctI GAATGC 1 cut(s) 1279
PfeI GAWTC 3 cut(s) 498, 839, 1118
PkrI GCNGC 9 cut(s) 52, 55, 72, 75, 266, 544, 731, 884, 996
PleI GAGTC 1 cut(s) 304
PpsI GAGTC 1 cut(s) 304
PshAI GACNNNNGTC 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 98
Psp6I CCWGG 2 cut(s) 415, 925
PspGI CCWGG 2 cut(s) 415, 925
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 1 cut(s) 282
PsuI RGATCY 1 cut(s) 1429
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
SacI GAGCTC 1 cut(s) 98
SaqAI TTAA 4 cut(s) 363, 1097, 1308, 1526
SatI GCNGC 9 cut(s) 51, 54, 71, 74, 265, 543, 730, 883, 995
Sau3AI GATC 6 cut(s) 4, 124, 140, 313, 1207, 1429
Sau96I GGNCC 1 cut(s) 282
SchI GAGTC 1 cut(s) 305
ScrFI CCNGG 2 cut(s) 417, 927
SduI GDGCHC 3 cut(s) 38, 98, 801
SfaNI GCATC 4 cut(s) 38, 403, 964, 1286
SmlI CTYRAG 3 cut(s) 985, 1260, 1525
SmoI CTYRAG 3 cut(s) 985, 1260, 1525
Sse9I AATT 8 cut(s) 8, 517, 603, 743, 833, 1179, 1194, 1239
SseBI AGGCCT 1 cut(s) 709
SsiI CCGC 7 cut(s) 70, 73, 262, 285, 289, 882, 1003
SspMI CTAG 6 cut(s) 99, 480, 915, 960, 1052, 1433
SstI GAGCTC 1 cut(s) 98
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 2 cut(s) 415, 925
StyI CCWWGG 3 cut(s) 77, 468, 1144
TaaI ACNGT 4 cut(s) 670, 721, 786, 1559
TaqI TCGA 2 cut(s) 486, 1175
TaqII GACCGA 1 cut(s) 704
TasI AATT 8 cut(s) 8, 517, 603, 743, 833, 1179, 1194, 1239
TauI GCSGC 3 cut(s) 73, 76, 885
TfiI GAWTC 3 cut(s) 498, 839, 1118
Tru1I TTAA 4 cut(s) 363, 1097, 1308, 1526
Tru9I TTAA 4 cut(s) 363, 1097, 1308, 1526
TscAI CASTG 9 cut(s) 574, 589, 789, 832, 876, 1019, 1217, 1344, 1562
TseFI GTSAC 3 cut(s) 472, 584, 827
TseI GCWGC 6 cut(s) 50, 53, 264, 542, 729, 994
Tsp45I GTSAC 3 cut(s) 472, 584, 827
TspDTI ATGAA 1 cut(s) 94
TspGWI ACGGA 4 cut(s) 211, 250, 387, 1150
TspRI CASTG 9 cut(s) 574, 589, 789, 832, 876, 1019, 1217, 1344, 1562
Vha464I CTTAAG 1 cut(s) 1525
XapI RAATTY 2 cut(s) 743, 1194
XceI RCATGY 1 cut(s) 414
XmiI GTMKAC 1 cut(s) 679
XspI CTAG 6 cut(s) 99, 480, 915, 960, 1052, 1433
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.