AT2G44200

pre-mRNA-splicing factor CWC25 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006065
Physical Location & Seq
Forward (+)
18276152 .. 18278627
2476 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G44200.1

Sequence Viewer

Length: 1482 bp
ATGGGGATGAAATTTCTGAACAAGAAGGGTTGGCATACGGGGAGTCTCCGTAACATCGAGACTGTATGGAAGGCTGAGCAGAAACAAGAGGCTGAGCAGAAAAAGCTTGAAGAGCTTCGTCTCCAGATCTTGCAGGAGAGGGAGCGTTCTGAGTTTCGTGCTCTCCAGGAACAAGCCGGTCTCATCCCGAGACAAGAGAGATTAGAGTTTCTGTATGATTCTGGATTAGCTGTAGGGAAGGGAAGTGCGAGTGGTTCAGGTGTTTCGTTTCAGAAAGAAGAGCAGCCTTTAGCCAAATCTGAAGCTGGTAGTAGTGCCAGTGAGAAGCCAGATCAGTCAGCTCCTGGTGCGCTGTTTGAGGAGAAGGCACAATCTGCTAATGATTCGTGGAGGAAACTTCACTCTGATCCTTTGCTTCTCATCAGGCAGCGTGAACAGGAAGCTCTTGCCAAAATCAAGAATAATCCTGTCAAAATGGCTCTTATTCGTAAATCGGTAGAAGAAAAAGGAAAGGGTAAAGATGGAGACACAAAGGAGCACAAGAAGAAGCATAAGCGTAAAAGTGGAAAGCATCAGAAGCAATCTTCATCCAGACAGCGGTCTGATTCGGAAGAAGATTCTGGTGAAGAGAATAACGGAAGAAAATCTCACCATCAGAAAACGTCAGGGACTCATGACAGACACTATGAAAGACCAAGGTCAGATTTAGAAGATGAGTCAAAAGGAAGAGAGAGTCGTGATAGGCACTATGAGAAACGAAGGTCAGAACTAGATGATGGGCACAAAAGAAGAGAGAGACATGATACGCACTATGAGAGACGAAGGTCAGAAATGGATGATGAGTCAAAAAGAAGAGAAAGTAGGGATAATCACTATGAGAGACGAAGGTCAGATTTGGATGATGAGTCCAAAAGAAGAGAAAGTCATGATAAGCACTTTGAGAGACAAAGGTCAGATTTGGATGATGAGTACAAAAGAAGAGAAAGTCAAGATAAGAGACGAAGGTCAGATATTGATGATGAACCAAAAAGAAGAGATGCTCGACCGAATGAGAAATATCGAAATCGCTCCCCTAAAGGCGGTGTGGAAAGGGAAAATCTTAAGAGTTATGGTCAAGAGGATAAAAAGAGGAAAGCAGAGGATTTAGACAGTGGAAAACCGAATGAATACCAGAATAGACGCCGGAAAGGTGGCTCTAAGCTATCAGAAGAAGAAAGAGCTGCTAGATTGAAGCAAATGCAAATGGATGCAGAGGTGCACGAGGAGCAAAGATGGACAAGATTGAAGAAAGCCGATGAGACCGATGCAGTGGAAGCTGATAAGAACAAAGTATCCACGGGCAAGAGCTTTTTGGACGATGCTAATAAGAGTGTGTATGGAGTTGAGAAAGGCGGAAGCTCAACAATTGAAGAAAGCGTGCGTCGCAGAAGCTATTATTCACAACGTGGAACTGCAGCTGAAGGCAATGCGTTTCGACGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CWC25 Cir_N

Protein Analysis

493

Amino Acids

57.78

Weight (kDa)

9.52

Isoelectric Point (pI)

85.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cir_N PF10197 11 - 46 4.8e-14 N-terminal domain of CBF1 interacting co-repressor CIR
CWC25 PF12542 64 - 156 5.6e-15 Pre-mRNA splicing factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 598, 1080, 1392
AclWI GGATC 1 cut(s) 401
AcsI RAATTY 1 cut(s) 11
AcuI CTGAAG 2 cut(s) 321, 1479
AcyI GRCGYC 1 cut(s) 1180
AdeI CACNNNGTG 1 cut(s) 1445
AfaI GTAC 1 cut(s) 971
AfiI CCNNNNNNNGG 2 cut(s) 597, 1079
AflII CTTAAG 1 cut(s) 1100
AgsI TTSAA 4 cut(s) 110, 1231, 1285, 1409
AjnI CCWGG 2 cut(s) 165, 343
AloI GAACNNNNNNTCC 2 cut(s) 1316, 1348
Alw21I GWGCWC 3 cut(s) 163, 540, 1260
Alw44I GTGCAC 1 cut(s) 1256
AlwI GGATC 1 cut(s) 401
AlwNI CAGNNNCTG 1 cut(s) 344
Ama87I CYCGRG 1 cut(s) 187
ApaLI GTGCAC 1 cut(s) 1256
ApeKI GCWGC 4 cut(s) 283, 427, 1220, 1454
ApoI RAATTY 1 cut(s) 11
Asp700I GAANNNNTTC 1 cut(s) 114
AspLEI GCGC 1 cut(s) 352
AsuHPI GGTGA 2 cut(s) 635, 641
AvaI CYCGRG 1 cut(s) 187
BaeGI GKGCMC 2 cut(s) 783, 1260
BauI CACGAG 1 cut(s) 1259
Bbv12I GWGCWC 3 cut(s) 163, 540, 1260
BbvI GCAGC 4 cut(s) 295, 439, 1207, 1466
BccI CCATC 4 cut(s) 515, 660, 770, 1266
BciT130I CCWGG 2 cut(s) 167, 345
BciVI GTATCC 1 cut(s) 1342
BfaI CTAG 2 cut(s) 770, 1224
BfmI CTRYAG 2 cut(s) 231, 1452
BfrI CTTAAG 1 cut(s) 1100
BfuI GTATCC 1 cut(s) 1342
BglII AGATCT 1 cut(s) 126
BisI GCNGC 4 cut(s) 284, 428, 1221, 1455
BlpI GCTNAGC 2 cut(s) 75, 93
BlsI GCNGC 4 cut(s) 285, 429, 1222, 1456
Bme1390I CCNGG 2 cut(s) 167, 345
BmeT110I CYCGRG 1 cut(s) 187
BmrFI CCNGG 2 cut(s) 167, 345
BmsI GCATC 5 cut(s) 580, 1027, 1237, 1294, 1348
BoxI GACNNNNGTC 6 cut(s) 598, 697, 823, 886, 949, 1003
BpmI CTGGAG 2 cut(s) 107, 149
Bpu1102I GCTNAGC 2 cut(s) 75, 93
BsaHI GRCGYC 1 cut(s) 1180
BsaI GGTCTC 2 cut(s) 185, 1292
BsaJI CCNNGG 2 cut(s) 695, 1335
BsaXI ACNNNNNCTCC 2 cut(s) 34, 64
Bsc4I CCNNNNNNNGG 2 cut(s) 597, 1079
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 1471
BseBI CCWGG 2 cut(s) 167, 345
BseDI CCNNGG 2 cut(s) 695, 1335
BseGI GGATG 7 cut(s) 12, 183, 587, 841, 904, 967, 1252
BseLI CCNNNNNNNGG 2 cut(s) 597, 1079
BseMI GCAATG 1 cut(s) 1471
BseMII CTCAG 3 cut(s) 66, 84, 141
BseNI ACTGG 1 cut(s) 318
BseRI GAGGAG 2 cut(s) 374, 1277
BseSI GKGCMC 2 cut(s) 783, 1260
BseXI GCAGC 4 cut(s) 295, 439, 1207, 1466
Bsh1285I CGRYCG 1 cut(s) 1046
BsiEI CGRYCG 1 cut(s) 1046
BsiHKAI GWGCWC 3 cut(s) 163, 540, 1260
BsiHKCI CYCGRG 1 cut(s) 187
BsiSI CCGG 2 cut(s) 177, 1183
BslFI GGGAC 1 cut(s) 682
BslI CCNNNNNNNGG 2 cut(s) 597, 1079
BsmBI CGTCTC 4 cut(s) 125, 811, 874, 991
BsmFI GGGAC 1 cut(s) 682
Bso31I GGTCTC 2 cut(s) 185, 1292
BsoBI CYCGRG 1 cut(s) 187
Bsp1286I GDGCHC 4 cut(s) 163, 540, 783, 1260
Bsp143I GATC 3 cut(s) 126, 331, 406
Bsp1720I GCTNAGC 2 cut(s) 75, 93
BspACI CCGC 3 cut(s) 598, 1080, 1392
BspCNI CTCAG 3 cut(s) 67, 85, 142
BspHI TCATGA 2 cut(s) 673, 925
BspMAI CTGCAG 1 cut(s) 1456
BspPI GGATC 1 cut(s) 401
BspQI GCTCTTC 2 cut(s) 105, 273
BspTI CTTAAG 1 cut(s) 1100
BspTNI GGTCTC 2 cut(s) 185, 1292
BsrDI GCAATG 1 cut(s) 1471
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 1 cut(s) 318
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 2 cut(s) 695, 1335
BssMI GATC 3 cut(s) 126, 331, 406
BssNI GRCGYC 1 cut(s) 1180
BssSI CACGAG 1 cut(s) 1259
BssT1I CCWWGG 1 cut(s) 695
Bst2BI CACGAG 1 cut(s) 1259
Bst2UI CCWGG 2 cut(s) 167, 345
Bst4CI ACNGT 2 cut(s) 64, 1151
Bst6I CTCTTC 9 cut(s) 105, 273, 621, 721, 784, 847, 910, 973, 1027
BstACI GRCGYC 1 cut(s) 1180
BstAFI CTTAAG 1 cut(s) 1100
BstAPI GCANNNNNTGC 1 cut(s) 374
BstC8I GCNNGC 1 cut(s) 1418
BstDEI CTNAG 4 cut(s) 75, 93, 150, 1197
BstDSI CCRYGG 1 cut(s) 1335
BstF5I GGATG 7 cut(s) 12, 183, 587, 841, 904, 967, 1252
BstHHI GCGC 1 cut(s) 352
BstKTI GATC 3 cut(s) 129, 334, 409
BstMBI GATC 3 cut(s) 126, 331, 406
BstMCI CGRYCG 1 cut(s) 1046
BstMWI GCNNNNNNNGC 8 cut(s) 103, 112, 347, 374, 577, 1264, 1313, 1422
BstNI CCWGG 2 cut(s) 167, 345
BstPAI GACNNNNGTC 6 cut(s) 598, 697, 823, 886, 949, 1003
BstSCI CCNGG 2 cut(s) 165, 343
BstSFI CTRYAG 2 cut(s) 231, 1452
BstSLI GKGCMC 2 cut(s) 783, 1260
BstV1I GCAGC 4 cut(s) 295, 439, 1207, 1466
BstX2I RGATCY 1 cut(s) 126
BstYI RGATCY 1 cut(s) 126
BsuI GTATCC 1 cut(s) 1342
BtgI CCRYGG 1 cut(s) 1335
BtsCI GGATG 7 cut(s) 12, 183, 587, 841, 904, 967, 1252
BtsI GCAGTG 1 cut(s) 1314
BtsIMutI CAGTG 3 cut(s) 325, 1156, 1314
Cac8I GCNNGC 1 cut(s) 1418
CaiI CAGNNNCTG 1 cut(s) 344
CciI TCATGA 2 cut(s) 673, 925
CfoI GCGC 1 cut(s) 352
Cfr10I RCCGGY 1 cut(s) 176
CseI GACGC 2 cut(s) 1188, 1409
Csp6I GTAC 1 cut(s) 970
CviAII CATG 3 cut(s) 674, 800, 926
CviQI GTAC 1 cut(s) 970
DdeI CTNAG 4 cut(s) 75, 93, 150, 1197
DpnI GATC 3 cut(s) 128, 333, 408
DpnII GATC 3 cut(s) 126, 331, 406
DraIII CACNNNGTG 1 cut(s) 1445
Eam1104I CTCTTC 9 cut(s) 105, 273, 621, 721, 784, 847, 910, 973, 1027
EarI CTCTTC 9 cut(s) 105, 273, 621, 721, 784, 847, 910, 973, 1027
EciI GGCGGA 1 cut(s) 1407
Eco130I CCWWGG 1 cut(s) 695
Eco31I GGTCTC 2 cut(s) 185, 1292
Eco57I CTGAAG 2 cut(s) 321, 1479
Eco88I CYCGRG 1 cut(s) 187
EcoRII CCWGG 2 cut(s) 165, 343
EcoT14I CCWWGG 1 cut(s) 695
ErhI CCWWGG 1 cut(s) 695
Esp3I CGTCTC 4 cut(s) 125, 811, 874, 991
FaeI CATG 3 cut(s) 677, 803, 929
FaqI GGGAC 1 cut(s) 682
FatI CATG 3 cut(s) 673, 799, 925
Fnu4HI GCNGC 4 cut(s) 284, 428, 1221, 1455
FokI GGATG 7 cut(s) 19, 170, 574, 848, 911, 974, 1259
Fsp4HI GCNGC 4 cut(s) 284, 428, 1221, 1455
FspBI CTAG 2 cut(s) 770, 1224
GlaI GCGC 1 cut(s) 351
GluI GCNGC 4 cut(s) 284, 428, 1221, 1455
GsuI CTGGAG 2 cut(s) 107, 149
HapII CCGG 2 cut(s) 177, 1183
HgaI GACGC 2 cut(s) 1188, 1409
HhaI GCGC 1 cut(s) 352
Hin1I GRCGYC 1 cut(s) 1180
Hin1II CATG 3 cut(s) 677, 803, 929
Hin6I GCGC 1 cut(s) 350
HinP1I GCGC 1 cut(s) 350
HindIII AAGCTT 1 cut(s) 104
HpaII CCGG 2 cut(s) 177, 1183
HphI GGTGA 2 cut(s) 635, 641
Hpy166II GTNNAC 2 cut(s) 434, 1258
Hpy8I GTNNAC 2 cut(s) 434, 1258
Hpy99I CGWCG 2 cut(s) 1425, 1479
HpyAV CCTTC 9 cut(s) 19, 64, 232, 358, 753, 816, 879, 996, 1454
HpyCH4III ACNGT 2 cut(s) 64, 1151
HpyCH4IV ACGT 2 cut(s) 662, 1444
HpyCH4V TGCA 6 cut(s) 133, 1240, 1250, 1258, 1307, 1454
HpyF10VI GCNNNNNNNGC 8 cut(s) 103, 112, 347, 374, 577, 1264, 1313, 1422
HpyF3I CTNAG 4 cut(s) 75, 93, 150, 1197
HpySE526I ACGT 2 cut(s) 662, 1444
Hsp92I GRCGYC 1 cut(s) 1180
Hsp92II CATG 3 cut(s) 677, 803, 929
HspAI GCGC 1 cut(s) 350
Kzo9I GATC 3 cut(s) 126, 331, 406
LguI GCTCTTC 2 cut(s) 105, 273
LmnI GCTCC 5 cut(s) 142, 346, 535, 1073, 1264
Lsp1109I GCAGC 4 cut(s) 295, 439, 1207, 1466
LweI GCATC 5 cut(s) 580, 1027, 1237, 1294, 1348
MaeI CTAG 2 cut(s) 770, 1224
MaeII ACGT 2 cut(s) 662, 1444
MaeIII GTNAC 1 cut(s) 50
MalI GATC 3 cut(s) 128, 333, 408
MboI GATC 3 cut(s) 126, 331, 406
MfeI CAATTG 1 cut(s) 1404
MflI RGATCY 1 cut(s) 126
MhlI GDGCHC 4 cut(s) 163, 540, 783, 1260
MluCI AATT 2 cut(s) 11, 1404
MlyI GAGTC 6 cut(s) 52, 664, 725, 742, 851, 914
MnlI CCTC 9 cut(s) 82, 132, 352, 384, 1111, 1122, 1132, 1246, 1255
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 1 cut(s) 1101
MspA1I CMGCKG 2 cut(s) 598, 1457
MspCI CTTAAG 1 cut(s) 1100
MspI CCGG 2 cut(s) 177, 1183
MspR9I CCNGG 2 cut(s) 167, 345
MunI CAATTG 1 cut(s) 1404
MvaI CCWGG 2 cut(s) 167, 345
MwoI GCNNNNNNNGC 8 cut(s) 103, 112, 347, 374, 577, 1264, 1313, 1422
NdeII GATC 3 cut(s) 126, 331, 406
NlaIII CATG 3 cut(s) 677, 803, 929
PagI TCATGA 2 cut(s) 673, 925
PciSI GCTCTTC 2 cut(s) 105, 273
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 4 cut(s) 218, 383, 605, 617
PfoI TCCNGGA 1 cut(s) 165
PkrI GCNGC 4 cut(s) 285, 429, 1222, 1456
PleI GAGTC 6 cut(s) 51, 664, 724, 741, 850, 913
PpsI GAGTC 6 cut(s) 51, 664, 724, 741, 850, 913
PshAI GACNNNNGTC 6 cut(s) 598, 697, 823, 886, 949, 1003
Psp6I CCWGG 2 cut(s) 165, 343
PspGI CCWGG 2 cut(s) 165, 343
PstI CTGCAG 1 cut(s) 1456
PstNI CAGNNNCTG 1 cut(s) 344
PsuI RGATCY 1 cut(s) 126
PvuII CAGCTG 1 cut(s) 1457
RsaI GTAC 1 cut(s) 971
RsaNI GTAC 1 cut(s) 970
SapI GCTCTTC 2 cut(s) 105, 273
SaqAI TTAA 1 cut(s) 1101
SatI GCNGC 4 cut(s) 284, 428, 1221, 1455
Sau3AI GATC 3 cut(s) 126, 331, 406
SchI GAGTC 6 cut(s) 52, 664, 725, 742, 851, 914
ScrFI CCNGG 2 cut(s) 167, 345
SduI GDGCHC 4 cut(s) 163, 540, 783, 1260
SfaNI GCATC 5 cut(s) 580, 1027, 1237, 1294, 1348
SfcI CTRYAG 2 cut(s) 231, 1452
SmlI CTYRAG 1 cut(s) 1100
SmoI CTYRAG 1 cut(s) 1100
Sse9I AATT 2 cut(s) 11, 1404
SsiI CCGC 3 cut(s) 598, 1080, 1392
SspMI CTAG 2 cut(s) 770, 1224
StyD4I CCNGG 2 cut(s) 165, 343
StyI CCWWGG 1 cut(s) 695
TaaI ACNGT 2 cut(s) 64, 1151
TaiI ACGT 2 cut(s) 665, 1447
TaqI TCGA 4 cut(s) 57, 1042, 1060, 1474
TaqII GACCGA 2 cut(s) 1060, 1316
TasI AATT 2 cut(s) 11, 1404
TatI WGTACW 1 cut(s) 969
TfiI GAWTC 4 cut(s) 218, 383, 605, 617
Tru1I TTAA 1 cut(s) 1101
Tru9I TTAA 1 cut(s) 1101
TscAI CASTG 3 cut(s) 325, 1156, 1314
TseI GCWGC 4 cut(s) 283, 427, 1220, 1454
TspDTI ATGAA 5 cut(s) 23, 576, 702, 1035, 1179
TspGWI ACGGA 2 cut(s) 38, 651
TspRI CASTG 3 cut(s) 325, 1156, 1314
Vha464I CTTAAG 1 cut(s) 1100
VneI GTGCAC 1 cut(s) 1256
XapI RAATTY 1 cut(s) 11
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 2 cut(s) 770, 1224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.