Rorug04G0125000

Pre-mRNA-splicing factor cwc25

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0006065
Physical Location & Seq
Forward (+)
19793717 .. 19833118
39402 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0125000.1

Sequence Viewer

Length: 2235 bp
ATGGATTCTGTTCTTAGAACTAGTTTCGTTCCCTTGCTCCATTCTCAAGATGGTAGTCATTCATTTTCATACATTCCAAATTTCTTATCCGCATCTCCTCCTCACCCCAGCAACCAGAAAATGTTCAATGGGACAGCTGTTGCTCTTCACAATAATTCAATTGATGACAAGAGCACTTTCATGAAGGGAGGTCACCAAGAAAGCCAGAATAATGGCAAGCTACCGAAATCAAAACTGCTGAATTTCTCAGGAGAGAGGCCCTCAACTCCAATTCTGGACACCATAAACTATCCCATTCACATGAGAAATCTTTCTATTGAGGAACTTGGAATTTTAGCGGATGAACTTAGGGAAGAGATTGTTTACACAGTGTCAAAGATTGGGGGTCACCTGAGCTCAAGCCTAGGAGTTTCTGAGTTAACAGTTGCACTTCACCATGTTTTCAACACTCCTGAAGATAAGATAATTTGGGATGTTGGTCACCAGACCTACCCTCATAAGATTTTAACTGGTCGGAGGTCGAGAATGCAAACAATCCGACAGACTTGTGGGCTTTCAGGCTTCCCCAAGAGGGATGAGAGTGTGCATGATGCCTTTGGAGTTGGCCATAGTTCCACTAGCATTTCAGCTGCTCTAGGAATGGCAGTTGCTAGAGATTTGCAAGGCAAGAAGAATCATGTGATTGCAGTGATAGGAGATGGAGCCATGACAGGAGGACAGGCATATGAGGCATTGAACAATGCAGGCTACCTTGATTCTAATCTTATAATCATCTTGAATGACAACAAGCAAGTCTCTCTGCCAACTGCCACTGTCGACGGCCCTGCCCCTCCAGTTGGAGCTCTAAGCAAAGCACTCACAAGATTACAGTCTACTCAAAAGTTCCAACTGCTACGTGAAGCAGCAAAGGGTATGACCAAGAAAATTGGACACCAAGCACATGAAGTTGCTGCTAAAGTTGACTCCTACATGAGAGGAATGGTGGGTGGTTCTGGGGCATGTCTGTTTGAAGAACTTGGACTCCACTATATTGGTCCTGTGAATGGTCATAATGTCGAAGACCTTGTCTACATTTTCAACAACATTAAATCCTTACCAGTACCAGGGCCTGTTCTGATTCATGTCATCACTGAGAAAGGAAAAGGCTATGCTCCAGCTGAAGTTGCCCCTGATAAGATGCATGGTGTTGTAAAATTTGACACCAAGTCAGGGACTCAGTTGAAGGCGAAATCAAGTACTCATTCATACACGCAGTACTTTGCAGAGTCCTTAATTGCCGAAGCAAAGAGAGATGATAAAGTTGTAGCCATCCATGCTGCAATGGGAAGTGGTACTGGACTCAACTTGTTCCAGAAACAATTCCCAGACAGATGTTTCGATGTTGGGATAGCAGAACAGCATGCTATTACCTTTGCTGCTGGCCTAGCTGCTGAAGGCCTTAAACCTTTCTGTGCTATTTACTCTTCGTTTCTTCAGAGGGGTTATGATCAGGTGGTTCATGATGTAGACTTACAAAAGCTCCCAGTGAGATTTGCTATAGATAGGGCTGGTCTTGTAGGTGCAGATGGTCCAACACATTGCGGAGCCTTTGATACAACTTTCATGGCTTGTTTACCTAACATGGTGGTCATGGCCCCTTCTTCTGAGACTGAACTTATGCACATGGTTGCCACGGCTTCTGCCATAGATGATAGGCCTAGTTGCTTCCGATACCCTAGAGGAAACGGCATTGGGTCAATTCTTCCACCAGACAACAAAGGAACCCCATTGGAGGTTGGCAAGGGAAGGGTGTTAAGGGAAGGTTGTAGGGTGGCTATTTTGGGTTACGGAACAATAGTACAAAGCTGTATAGCAGCAGCTGACCTGCTTCAAGTTCTCAACATCTCCACAACTGTGGCTGATGCACGATTCTGCAAGCCTCTTGATGGAAATTTGATCAGACAGCTAGCTCAAGAACATGAGATTCTCATTACCGTTGAAGAAGGATCAATCGGAGGATTCAGCTCCCACGTTTCCCAATTCTTAGGCTTGAATGGATTACTTGATGGCAAGCTCAAGTGGAGGTCTATGATGCTTCCTGACAGATATATTGAACATGGATCTCAGAAAGACCAAACTGAAGCAGCAGGGTTGAGTTCCAGGCATATTGTAACAACTGTGGTGTCACTCCTAGGTGAACATAGGGATGTCCTTCACCTTCTCAACTTTCAGACCAATACTACACATAGCCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CWC25 Cir_N

Protein Analysis

744

Amino Acids

80.4

Weight (kDa)

6.82

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DXP_synthase_N PF13292 92 - 377 1.1e-108 1-deoxy-D-xylulose-5-phosphate synthase
E1_dh PF00676 202 - 263 6.1e-07 Dehydrogenase E1 component
Transket_pyr PF02779 415 - 575 5e-47 Transketolase, pyrimidine binding domain
Transketolase_C PF02780 593 - 716 4.1e-31 Transketolase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 767
Acc36I ACCTGC 1 cut(s) 1872
AccI GTMKAC 4 cut(s) 816, 872, 1068, 1506
AciI CCGC 3 cut(s) 90, 338, 1581
AclWI GGATC 2 cut(s) 1993, 2107
AcoI YGGCCR 1 cut(s) 604
AcsI RAATTY 5 cut(s) 79, 241, 330, 1193, 1930
AcuI CTGAAG 5 cut(s) 474, 1179, 1452, 1457, 2139
AfaI GTAC 5 cut(s) 1101, 1237, 1256, 1333, 1839
AfiI CCNNNNNNNGG 7 cut(s) 571, 836, 1043, 1103, 1209, 1771, 1925
AhdI GACNNNNNGTC 1 cut(s) 1204
AhlI ACTAGT 1 cut(s) 20
AjnI CCWGG 2 cut(s) 1102, 2138
AleI CACNNNNGTG 1 cut(s) 1892
AloI GAACNNNNNNTCC 2 cut(s) 1005, 1037
Alw21I GWGCWC 3 cut(s) 176, 398, 844
Alw26I GTCTC 2 cut(s) 799, 1640
AlwI GGATC 2 cut(s) 1993, 2107
AlwNI CAGNNNCTG 2 cut(s) 1109, 1859
AoxI GGCC 8 cut(s) 257, 604, 820, 1106, 1420, 1435, 1632, 1694
ApeKI GCWGC 9 cut(s) 629, 902, 950, 1316, 1415, 1427, 1853, 1856, 2123
ApoI RAATTY 5 cut(s) 79, 241, 330, 1193, 1930
ArsI GACNNNNNNTTYG 4 cut(s) 1050, 1082, 1358, 1390
Asp700I GAANNNNTTC 3 cut(s) 122, 310, 1358
AspA2I CCTAGG 2 cut(s) 403, 2170
AspS9I GGNCC 6 cut(s) 258, 821, 1034, 1106, 1568, 1633
AsuHPI GGTGA 7 cut(s) 95, 185, 380, 425, 473, 2186, 2186
AsuNHI GCTAGC 1 cut(s) 1945
AvaII GGWCC 2 cut(s) 1034, 1568
AvrII CCTAGG 2 cut(s) 403, 2170
BalI TGGCCA 1 cut(s) 606
BanII GRGCYC 2 cut(s) 398, 844
BbsI GAAGAC 1 cut(s) 1065
Bbv12I GWGCWC 3 cut(s) 176, 398, 844
BbvI GCAGC 9 cut(s) 616, 914, 937, 1303, 1402, 1414, 1865, 1868, 2135
BccI CCATC 6 cut(s) 44, 692, 1316, 1559, 1919, 2039
BceAI ACGGC 3 cut(s) 835, 1689, 1741
BcgI CGANNNNNNTGC 2 cut(s) 806, 840
BciT130I CCWGG 2 cut(s) 1104, 2140
BclI TGATCA 2 cut(s) 1486, 1935
BcoDI GTCTC 2 cut(s) 799, 1640
BcuI ACTAGT 1 cut(s) 20
BfmI CTRYAG 1 cut(s) 1536
BfuAI ACCTGC 1 cut(s) 1872
BisI GCNGC 9 cut(s) 630, 903, 951, 1317, 1416, 1428, 1854, 1857, 2124
BlnI CCTAGG 2 cut(s) 403, 2170
BlsI GCNGC 9 cut(s) 631, 904, 952, 1318, 1417, 1429, 1855, 1858, 2125
BmcAI AGTACT 2 cut(s) 1237, 1256
Bme1390I CCNGG 2 cut(s) 1104, 2140
Bme18I GGWCC 2 cut(s) 1034, 1568
BmeRI GACNNNNNGTC 1 cut(s) 1204
BmgT120I GGNCC 6 cut(s) 258, 821, 1034, 1106, 1568, 1633
BmiI GGNNCC 4 cut(s) 703, 1585, 1635, 1762
BmrFI CCNGG 2 cut(s) 1104, 2140
BmrI ACTGGG 1 cut(s) 1517
BmsI GCATC 5 cut(s) 101, 580, 1167, 1891, 2061
BmtI GCTAGC 1 cut(s) 1949
BmuI ACTGGG 1 cut(s) 1517
BpiI GAAGAC 1 cut(s) 1065
BplI GAGNNNNNCTC 2 cut(s) 245, 277
BpmI CTGGAG 2 cut(s) 816, 1137
Bpu10I CCTNAGC 1 cut(s) 392
BpuEI CTTGAG 4 cut(s) 30, 382, 1935, 2039
BsaAI YACGTR 1 cut(s) 896
BsaBI GATNNNNATC 1 cut(s) 759
BsaJI CCNNGG 4 cut(s) 403, 1103, 1671, 2170
BsaXI ACNNNNNCTCC 4 cut(s) 1005, 1035, 1503, 1533
Bsc4I CCNNNNNNNGG 7 cut(s) 571, 836, 1043, 1103, 1209, 1771, 1925
Bse1I ACTGG 5 cut(s) 514, 833, 1097, 1339, 1523
Bse3DI GCAATG 2 cut(s) 1326, 1576
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 2 cut(s) 1104, 2140
BseDI CCNNGG 4 cut(s) 403, 1103, 1671, 2170
BseGI GGATG 5 cut(s) 346, 478, 580, 1308, 2191
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 7 cut(s) 571, 836, 1043, 1103, 1209, 1771, 1925
BseMI GCAATG 2 cut(s) 1326, 1576
BseMII CTCAG 7 cut(s) 261, 383, 405, 1122, 1229, 1635, 2117
BseNI ACTGG 5 cut(s) 514, 833, 1097, 1339, 1523
BseRI GAGGAG 2 cut(s) 87, 90
BseXI GCAGC 9 cut(s) 616, 914, 937, 1303, 1402, 1414, 1865, 1868, 2135
BseYI CCCAGC 1 cut(s) 107
BsgI GTGCAG 1 cut(s) 1581
BshFI GGCC 8 cut(s) 259, 606, 822, 1108, 1422, 1437, 1634, 1696
BsiHKAI GWGCWC 3 cut(s) 176, 398, 844
BslFI GGGAC 2 cut(s) 145, 1225
BslI CCNNNNNNNGG 7 cut(s) 571, 836, 1043, 1103, 1209, 1771, 1925
BsmAI GTCTC 2 cut(s) 799, 1640
BsmFI GGGAC 2 cut(s) 145, 1225
BsmI GAATGC 1 cut(s) 531
BsnI GGCC 8 cut(s) 259, 606, 822, 1108, 1422, 1437, 1634, 1696
Bsp1286I GDGCHC 3 cut(s) 176, 398, 844
Bsp143I GATC 4 cut(s) 1486, 1935, 1985, 2099
BspACI CCGC 3 cut(s) 90, 338, 1581
BspANI GGCC 8 cut(s) 259, 606, 822, 1108, 1422, 1437, 1634, 1696
BspCNI CTCAG 7 cut(s) 260, 384, 406, 1123, 1228, 1636, 2116
BspHI TCATGA 2 cut(s) 180, 1498
BspLI GGNNCC 4 cut(s) 703, 1585, 1635, 1762
BspMI ACCTGC 1 cut(s) 1872
BspOI GCTAGC 1 cut(s) 1949
BspPI GGATC 2 cut(s) 1993, 2107
BspQI GCTCTTC 1 cut(s) 150
BsrDI GCAATG 2 cut(s) 1326, 1576
BsrI ACTGG 5 cut(s) 514, 833, 1097, 1339, 1523
BssECI CCNNGG 4 cut(s) 403, 1103, 1671, 2170
BssMI GATC 4 cut(s) 1486, 1935, 1985, 2099
BssT1I CCWWGG 2 cut(s) 403, 2170
Bst2UI CCWGG 2 cut(s) 1104, 2140
Bst4CI ACNGT 7 cut(s) 370, 424, 814, 870, 1894, 1975, 2158
Bst6I CTCTTC 3 cut(s) 150, 348, 1468
BstBAI YACGTR 1 cut(s) 896
BstC8I GCNNGC 7 cut(s) 218, 745, 1401, 1420, 1916, 1947, 2051
BstDSI CCRYGG 1 cut(s) 1671
BstEII GGTNACC 3 cut(s) 191, 386, 479
BstF5I GGATG 5 cut(s) 346, 478, 580, 1308, 2191
BstKTI GATC 4 cut(s) 1489, 1938, 1988, 2102
BstMAI GTCTC 2 cut(s) 799, 1640
BstMBI GATC 4 cut(s) 1486, 1935, 1985, 2099
BstMWI GCNNNNNNNGC 4 cut(s) 728, 1163, 1313, 1424
BstNI CCWGG 2 cut(s) 1104, 2140
BstNSI RCATGY 2 cut(s) 1002, 1403
BstPI GGTNACC 3 cut(s) 191, 386, 479
BstSCI CCNGG 2 cut(s) 1102, 2138
BstSFI CTRYAG 1 cut(s) 1536
BstV1I GCAGC 9 cut(s) 616, 914, 937, 1303, 1402, 1414, 1865, 1868, 2135
BstV2I GAAGAC 1 cut(s) 1065
BstX2I RGATCY 1 cut(s) 2099
BstXI CCANNNNNNTGG 3 cut(s) 212, 1031, 1894
BstYI RGATCY 1 cut(s) 2099
BsuRI GGCC 8 cut(s) 259, 606, 822, 1108, 1422, 1437, 1634, 1696
BtgI CCRYGG 1 cut(s) 1671
BtsCI GGATG 5 cut(s) 346, 478, 580, 1308, 2191
BtsI GCAGTG 1 cut(s) 693
BtsIMutI CAGTG 5 cut(s) 375, 693, 810, 1128, 1530
BveI ACCTGC 1 cut(s) 1872
Cac8I GCNNGC 7 cut(s) 218, 745, 1401, 1420, 1916, 1947, 2051
CaiI CAGNNNCTG 2 cut(s) 1109, 1859
CciI TCATGA 2 cut(s) 180, 1498
Cfr13I GGNCC 6 cut(s) 258, 821, 1034, 1106, 1568, 1633
Csp6I GTAC 5 cut(s) 1100, 1236, 1255, 1332, 1838
CviQI GTAC 5 cut(s) 1100, 1236, 1255, 1332, 1838
DpnI GATC 4 cut(s) 1488, 1937, 1987, 2101
DpnII GATC 4 cut(s) 1486, 1935, 1985, 2099
DriI GACNNNNNGTC 1 cut(s) 1204
EaeI YGGCCR 1 cut(s) 604
Eam1104I CTCTTC 3 cut(s) 150, 348, 1468
Eam1105I GACNNNNNGTC 1 cut(s) 1204
EarI CTCTTC 3 cut(s) 150, 348, 1468
Ecl136II GAGCTC 2 cut(s) 396, 842
Eco130I CCWWGG 2 cut(s) 403, 2170
Eco147I AGGCCT 2 cut(s) 1437, 1696
Eco24I GRGCYC 2 cut(s) 398, 844
Eco47I GGWCC 2 cut(s) 1034, 1568
Eco53kI GAGCTC 2 cut(s) 396, 842
Eco57I CTGAAG 5 cut(s) 474, 1179, 1452, 1457, 2139
Eco91I GGTNACC 3 cut(s) 191, 386, 479
EcoICRI GAGCTC 2 cut(s) 396, 842
EcoO109I RGGNCCY 2 cut(s) 258, 1106
EcoO65I GGTNACC 3 cut(s) 191, 386, 479
EcoRII CCWGG 2 cut(s) 1102, 2138
EcoT14I CCWWGG 2 cut(s) 403, 2170
EcoT22I ATGCAT 1 cut(s) 1182
EcoT38I GRGCYC 2 cut(s) 398, 844
ErhI CCWWGG 2 cut(s) 403, 2170
FaqI GGGAC 2 cut(s) 145, 1225
FauNDI CATATG 1 cut(s) 724
FbaI TGATCA 2 cut(s) 1486, 1935
FblI GTMKAC 4 cut(s) 816, 872, 1068, 1506
Fnu4HI GCNGC 9 cut(s) 630, 903, 951, 1317, 1416, 1428, 1854, 1857, 2124
FokI GGATG 5 cut(s) 353, 485, 587, 1295, 2198
FriOI GRGCYC 2 cut(s) 398, 844
Fsp4HI GCNGC 9 cut(s) 630, 903, 951, 1317, 1416, 1428, 1854, 1857, 2124
GluI GCNGC 9 cut(s) 630, 903, 951, 1317, 1416, 1428, 1854, 1857, 2124
GsaI CCCAGC 1 cut(s) 111
GsuI CTGGAG 2 cut(s) 816, 1137
HaeIII GGCC 8 cut(s) 259, 606, 822, 1108, 1422, 1437, 1634, 1696
HincII GTYRAC 3 cut(s) 420, 817, 961
HindII GTYRAC 3 cut(s) 420, 817, 961
HpaI GTTAAC 1 cut(s) 420
HphI GGTGA 7 cut(s) 95, 185, 380, 425, 473, 2186, 2186
Hpy166II GTNNAC 9 cut(s) 364, 420, 817, 873, 961, 1069, 1507, 1613, 2177
Hpy8I GTNNAC 9 cut(s) 364, 420, 817, 873, 961, 1069, 1507, 1613, 2177
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 9 cut(s) 178, 1216, 1427, 1647, 1779, 1793, 1976, 2201, 2207
HpyCH4III ACNGT 7 cut(s) 370, 424, 814, 870, 1894, 1975, 2158
HpyCH4IV ACGT 2 cut(s) 895, 2010
HpyF10VI GCNNNNNNNGC 4 cut(s) 728, 1163, 1313, 1424
HpySE526I ACGT 2 cut(s) 895, 2010
Ksp22I TGATCA 2 cut(s) 1486, 1935
KspAI GTTAAC 1 cut(s) 420
Kzo9I GATC 4 cut(s) 1486, 1935, 1985, 2099
LguI GCTCTTC 1 cut(s) 150
LmnI GCTCC 7 cut(s) 42, 701, 839, 1156, 1524, 1583, 2009
Lsp1109I GCAGC 9 cut(s) 616, 914, 937, 1303, 1402, 1414, 1865, 1868, 2135
LweI GCATC 5 cut(s) 101, 580, 1167, 1891, 2061
MaeII ACGT 2 cut(s) 895, 2010
MaeIII GTNAC 6 cut(s) 191, 386, 479, 1823, 2149, 2163
MalI GATC 4 cut(s) 1488, 1937, 1987, 2101
MboI GATC 4 cut(s) 1486, 1935, 1985, 2099
MfeI CAATTG 1 cut(s) 159
MflI RGATCY 1 cut(s) 2099
MhlI GDGCHC 3 cut(s) 176, 398, 844
MlsI TGGCCA 1 cut(s) 606
MluNI TGGCCA 1 cut(s) 606
MlyI GAGTC 5 cut(s) 956, 1014, 1207, 1274, 1332
MmeI TCCRAC 5 cut(s) 494, 562, 817, 910, 1595
Mox20I TGGCCA 1 cut(s) 606
Mph1103I ATGCAT 1 cut(s) 1182
MroXI GAANNNNTTC 3 cut(s) 122, 310, 1358
MscI TGGCCA 1 cut(s) 606
MseI TTAA 6 cut(s) 419, 506, 1086, 1271, 1440, 1793
MslI CAYNNNNRTG 4 cut(s) 179, 299, 1892, 2184
Msp20I TGGCCA 1 cut(s) 606
MspA1I CMGCKG 4 cut(s) 137, 629, 1157, 1859
MspR9I CCNGG 2 cut(s) 1104, 2140
MunI CAATTG 1 cut(s) 159
Mva1269I GAATGC 1 cut(s) 531
MvaI CCWGG 2 cut(s) 1104, 2140
MwoI GCNNNNNNNGC 4 cut(s) 728, 1163, 1313, 1424
NdeI CATATG 1 cut(s) 724
NdeII GATC 4 cut(s) 1486, 1935, 1985, 2099
NheI GCTAGC 1 cut(s) 1945
NlaIV GGNNCC 4 cut(s) 703, 1585, 1635, 1762
NmuCI GTSAC 4 cut(s) 191, 386, 479, 2163
NsiI ATGCAT 1 cut(s) 1182
NspI RCATGY 2 cut(s) 1002, 1403
OliI CACNNNNGTG 1 cut(s) 1892
PaeI GCATGC 1 cut(s) 1403
PagI TCATGA 2 cut(s) 180, 1498
PceI AGGCCT 2 cut(s) 1437, 1696
PciSI GCTCTTC 1 cut(s) 150
PctI GAATGC 1 cut(s) 531
PdmI GAANNNNTTC 3 cut(s) 122, 310, 1358
PfeI GAWTC 7 cut(s) 5, 673, 755, 1117, 1908, 1963, 1998
PflFI GACNNNGTC 1 cut(s) 1064
PkrI GCNGC 9 cut(s) 631, 904, 952, 1318, 1417, 1429, 1855, 1858, 2125
PleI GAGTC 5 cut(s) 956, 1014, 1207, 1273, 1332
PpsI GAGTC 5 cut(s) 956, 1014, 1207, 1273, 1332
Ppu21I YACGTR 1 cut(s) 896
PsiI TTATAA 1 cut(s) 767
Psp124BI GAGCTC 2 cut(s) 398, 844
Psp6I CCWGG 2 cut(s) 1102, 2138
PspEI GGTNACC 3 cut(s) 191, 386, 479
PspFI CCCAGC 1 cut(s) 107
PspGI CCWGG 2 cut(s) 1102, 2138
PspN4I GGNNCC 4 cut(s) 703, 1585, 1635, 1762
PspPI GGNCC 6 cut(s) 258, 821, 1034, 1106, 1568, 1633
PstNI CAGNNNCTG 2 cut(s) 1109, 1859
PsuI RGATCY 1 cut(s) 2099
PsyI GACNNNGTC 1 cut(s) 1064
PvuII CAGCTG 4 cut(s) 137, 629, 1157, 1859
RsaI GTAC 5 cut(s) 1101, 1237, 1256, 1333, 1839
RsaNI GTAC 5 cut(s) 1100, 1236, 1255, 1332, 1838
RseI CAYNNNNRTG 4 cut(s) 179, 299, 1892, 2184
SacI GAGCTC 2 cut(s) 398, 844
SalI GTCGAC 1 cut(s) 815
SapI GCTCTTC 1 cut(s) 150
SaqAI TTAA 6 cut(s) 419, 506, 1086, 1271, 1440, 1793
SatI GCNGC 9 cut(s) 630, 903, 951, 1317, 1416, 1428, 1854, 1857, 2124
Sau3AI GATC 4 cut(s) 1486, 1935, 1985, 2099
Sau96I GGNCC 6 cut(s) 258, 821, 1034, 1106, 1568, 1633
ScaI AGTACT 2 cut(s) 1237, 1256
SchI GAGTC 5 cut(s) 956, 1014, 1207, 1274, 1332
ScrFI CCNGG 2 cut(s) 1104, 2140
SduI GDGCHC 3 cut(s) 176, 398, 844
SfaNI GCATC 5 cut(s) 101, 580, 1167, 1891, 2061
SfcI CTRYAG 1 cut(s) 1536
SinI GGWCC 2 cut(s) 1034, 1568
SmiMI CAYNNNNRTG 4 cut(s) 179, 299, 1892, 2184
SmlI CTYRAG 4 cut(s) 45, 397, 1950, 2054
SmoI CTYRAG 4 cut(s) 45, 397, 1950, 2054
SpeI ACTAGT 1 cut(s) 20
SphI GCATGC 1 cut(s) 1403
SseBI AGGCCT 2 cut(s) 1437, 1696
SsiI CCGC 3 cut(s) 90, 338, 1581
SstI GAGCTC 2 cut(s) 398, 844
StuI AGGCCT 2 cut(s) 1437, 1696
StyD4I CCNGG 2 cut(s) 1102, 2138
StyI CCWWGG 2 cut(s) 403, 2170
TaaI ACNGT 7 cut(s) 370, 424, 814, 870, 1894, 1975, 2158
TaiI ACGT 2 cut(s) 898, 2013
TaqI TCGA 4 cut(s) 521, 816, 1056, 1377
TatI WGTACW 3 cut(s) 1235, 1254, 1837
TfiI GAWTC 7 cut(s) 5, 673, 755, 1117, 1908, 1963, 1998
Tru1I TTAA 6 cut(s) 419, 506, 1086, 1271, 1440, 1793
Tru9I TTAA 6 cut(s) 419, 506, 1086, 1271, 1440, 1793
TscAI CASTG 5 cut(s) 375, 693, 817, 1135, 1530
TseFI GTSAC 4 cut(s) 191, 386, 479, 2163
TseI GCWGC 9 cut(s) 629, 902, 950, 1316, 1415, 1427, 1853, 1856, 2123
Tsp45I GTSAC 4 cut(s) 191, 386, 479, 2163
TspGWI ACGGA 1 cut(s) 1842
TspRI CASTG 5 cut(s) 375, 693, 817, 1135, 1530
Tth111I GACNNNGTC 1 cut(s) 1064
VpaK11BI GGWCC 2 cut(s) 1034, 1568
XapI RAATTY 5 cut(s) 79, 241, 330, 1193, 1930
XceI RCATGY 2 cut(s) 1002, 1403
XcmI CCANNNNNNNNNTGG 1 cut(s) 47
XmaJI CCTAGG 2 cut(s) 403, 2170
XmiI GTMKAC 4 cut(s) 816, 872, 1068, 1506
XmnI GAANNNNTTC 3 cut(s) 122, 310, 1358
ZrmI AGTACT 2 cut(s) 1237, 1256
Zsp2I ATGCAT 1 cut(s) 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.