Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000005

Orthogroup ID

OG0006065

Physical Location & Seq

Genomic Coordinates Forward (+)

37891130 .. 37897098

Gene Structure 5969 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_5G00357930.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1647 bp

ATGATTGTAAGAGTTTCTGAACAAGAAGGGGTGGCACACCGGAGCCTCCGGAACATAGAGAACGTGTGGAAGGCGGAGCAGAAGCACGCCGCCGAAGAGAAGAAGCTCGACGAGCTCCGCAAACAGATCGTTGAAGAAAGAGAACGCTCCGAGTTCCGTGAGCTCCAGGAGCAAGCCGGCCTCGTCCCCAAGCAAGAGAGATTGGAGTTCCTCTACGATTCCGGATTGGCCGTAGGGCGCCCCGGCGGCTCTGATAATTTCAAGGAAGCCTTCACCAAAGCCGAAGAACCGTCTTCTTCCTCCGCAGCCGCCGCTGCCGCTGCCACCGCGAAGGAGGCATCATCGTCTGCGCCGGGGGCTCTGTTCGCAGCAGACAAGCCTCAGTCTGCCAATGATGCATGGAGGAAGCTTCACTCGGATCCTTTGCTTATGATTAAGCAGCGAGAGCAGGAAGCCCTCTCTCGGATCAAGAACAATCCTGTCCAGATGGCTATGATTCGCAAACAAGTTGAAGCAAAGAAGCCCAAAGAAAAAGAGCGTGACAAGAAGGAACGTCGGAAGAAACACCGTAAAGATTCTTCAAAGCATCGGAAGCAAACATCTGATTCAGAAGATGACACAGCTGATATGGAAAGGAGGAAGAATAATCATCGTAAGGCATCAAAATATGTTAAGCATTCAGAATCAGAGGATGAACCCCATGGAAAAGCGCATCGAAGTGAGAAGAACCATGTTAAGCAGTCAAATGTTGAAGATGAATCACGTTATAGAGCACGCCAAAGTGGGAAAACCCATGCCAAGTATTCAGATTCTGAAGGTGAATTGCGTAGCAAAGCACGTAGCAGTGATAAAAATCATGTTAGGCATTCAGATTTGGAGGATACATCACGTAACAGATCACATCATAGTGAGAAGAACCGTGTTAAGTATTCAGATTCAGAAGATGAGCCACATAACAGAGGGCGTGGAAGTGAGAAAAACCGTGTTAAGCATTCAGATTCAGAAGACAAGCTACACAACAGAGGTCGAAGTGAGAACAACCGTGGTAAGCATTCAGATTCAGAAGATGAGCTACATAACAGAAGAAGTCGAAGTGAGAGAAACCGTGGAAAGCATTCAGATTCAGAAGATGAACTACATAACAGAAGAAGTCGAGGTGAGAAGAATCGTGTCAAGCATTCAGATTCAGAAGACGGGCTACATAACAGAGCACGTCGAAGTGAGAAAGATGATGCTATAACAAGGGGAAAAGAGGATGGTCAAGGTATTGTCAAGGGAAAACATGAAAATTCAGAACAAGAAAACTATTCCATGAGAGGTCGAAATGAATCCCACAACAAGCGTCGGTTTGTTCCGCCTAAACTTTCAGAAGAAGAAAGAGCTGCTCGGCTAAAGGAGATGCAGATGGATGCTGAGTTGCATGAAGAACAAAGATGGAAACGTTTGAAGAAGGCTGACGAGAATGATGCACGGGAAGCTACCCAGGCTATCAGATCTGGCGGTAAGAATTTCTTGGATGCTGTTAAAACTAGTGTTTATGGTTCTGGGAAGGGAGGAAGCTCAACAATAGAGGAGAGTGTCCGTCGCCGAACCCATTATTTGCAGGGCAGGTCTGAAGCAGGTGAAAGCAATGCATTTCGACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CWC25 Cir_N

Protein Analysis

548

Amino Acids

63.13

Weight (kDa)

9.63

Isoelectric Point (pI)

70.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cir_N	PF10197	15 - 46	9.2e-09	N-terminal domain of CBF1 interacting co-repressor CIR
CWC25	PF12542	64 - 160	3.8e-15	Pre-mRNA splicing factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44195 AT2G44200
fragaria_vesca	FvH4_4g13451
malus_domestica	MD04G1014200.v1.1
prunus_persica	Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G162300_v2.0.a1
pyrus_communis	pycom04g01100
rosa_chinensis	RchiOBHm_Chr4g0414011
rosa_laevigata	RLG00000008185
rosa_multiflora	Rmu_sc0003264.1_g000022 Rmu_sc0006428.1_g000008
rosa_roxburghii	Rroxscaffold_5G00357930
rosa_rugosa	Rorug04G0125000
rosa_samantha	Rh4AG183500 Rh4BG183000 Rh4CG195500 Rh4DG178800
rosa_wichuraiana	Rw4G015600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1610
Acc36I	ACCTGC	2 cut(s)	1599, 1610
AccB1I	GGYRCC	1 cut(s)	237
AccII	CGCG	1 cut(s)	329
AccIII	TCCGGA	2 cut(s)	48, 221
AclI	AACGTT	1 cut(s)	1441
AclWI	GGATC	3 cut(s)	413, 426, 473
AcoI	YGGCCR	1 cut(s)	228
AcsI	RAATTY	2 cut(s)	1288, 1507
AcuI	CTGAAG	2 cut(s)	834, 1635
AcyI	GRCGYC	1 cut(s)	238
AfiI	CCNNNNNNNGG	1 cut(s)	462
AflIII	ACRYGT	1 cut(s)	63
AgsI	TTSAA	6 cut(s)	134, 262, 512, 582, 752, 1447
AhlI	ACTAGT	1 cut(s)	1529
AjiI	CACGTC	1 cut(s)	1214
AjnI	CCWGG	2 cut(s)	165, 1482
Alw21I	GWGCWC	4 cut(s)	117, 165, 775, 1213
AlwI	GGATC	3 cut(s)	413, 426, 473
AlwNI	CAGNNNCTG	1 cut(s)	812
Aor13HI	TCCGGA	2 cut(s)	48, 221
AoxI	GGCC	2 cut(s)	178, 228
ApeKI	GCWGC	6 cut(s)	305, 314, 320, 368, 439, 1382
ApoI	RAATTY	2 cut(s)	1288, 1507
Asp700I	GAANNNNTTC	1 cut(s)	1114
AspLEI	GCGC	3 cut(s)	240, 352, 712
AsuC2I	CCSGG	2 cut(s)	243, 354
AsuHPI	GGTGA	4 cut(s)	265, 830, 1169, 1634
BamHI	GGATCC	1 cut(s)	418
BanI	GGYRCC	1 cut(s)	237
BanII	GRGCYC	3 cut(s)	117, 165, 361
BbsI	GAAGAC	3 cut(s)	285, 1011, 1197
Bbv12I	GWGCWC	4 cut(s)	117, 165, 775, 1213
BbvI	GCAGC	6 cut(s)	301, 307, 317, 380, 451, 1369
BccI	CCATC	4 cut(s)	481, 1250, 1399, 1428
BceAI	ACGGC	1 cut(s)	215
BcgI	CGANNNNNNTGC	4 cut(s)	109, 143, 1448, 1482
BciT130I	CCWGG	2 cut(s)	167, 1484
BciVI	GTATCC	1 cut(s)	874
BcnI	CCSGG	2 cut(s)	243, 354
BcuI	ACTAGT	1 cut(s)	1529
BfaI	CTAG	1 cut(s)	1530
BfoI	RGCGCY	1 cut(s)	241
BfuAI	ACCTGC	2 cut(s)	1599, 1610
BfuI	GTATCC	1 cut(s)	874
BglI	GCCNNNNNGGC	1 cut(s)	246
BglII	AGATCT	1 cut(s)	1493
Bme1390I	CCNGG	4 cut(s)	167, 243, 354, 1484
BmgBI	CACGTC	1 cut(s)	1214
BmiI	GGNNCC	3 cut(s)	44, 239, 420
BmrFI	CCNGG	4 cut(s)	167, 243, 354, 1484
BpiI	GAAGAC	3 cut(s)	285, 1011, 1197
BpmI	CTGGAG	1 cut(s)	149
BpuMI	CCSGG	2 cut(s)	243, 354
BsaAI	YACGTR	2 cut(s)	839, 890
BsaBI	GATNNNNATC	1 cut(s)	852
BsaHI	GRCGYC	1 cut(s)	238
BsaJI	CCNNGG	6 cut(s)	241, 353, 700, 1042, 1105, 1482
BsaWI	WCCGGW	3 cut(s)	39, 48, 221
Bsc4I	CCNNNNNNNGG	1 cut(s)	462
Bse118I	RCCGGY	1 cut(s)	176
Bse3DI	GCAATG	1 cut(s)	1636
Bse8I	GATNNNNATC	1 cut(s)	852
BseAI	TCCGGA	2 cut(s)	48, 221
BseBI	CCWGG	2 cut(s)	167, 1484
BseDI	CCNNGG	6 cut(s)	241, 353, 700, 1042, 1105, 1482
BseGI	GGATG	4 cut(s)	697, 1261, 1414, 1522
BseJI	GATNNNNATC	1 cut(s)	852
BseLI	CCNNNNNNNGG	1 cut(s)	462
BseMI	GCAATG	1 cut(s)	1636
BseMII	CTCAG	2 cut(s)	395, 1404
BseRI	GAGGAG	1 cut(s)	1586
BseXI	GCAGC	6 cut(s)	301, 307, 317, 380, 451, 1369
Bsh1236I	CGCG	1 cut(s)	329
BshFI	GGCC	2 cut(s)	180, 230
BshNI	GGYRCC	1 cut(s)	237
BsiHKAI	GWGCWC	4 cut(s)	117, 165, 775, 1213
BsiSI	CCGG	6 cut(s)	40, 49, 177, 222, 243, 353
BslFI	GGGAC	1 cut(s)	170
BslI	CCNNNNNNNGG	1 cut(s)	462
BsmFI	GGGAC	1 cut(s)	170
BsmI	GAATGC	6 cut(s)	676, 865, 991, 1051, 1114, 1177
BsnI	GGCC	2 cut(s)	180, 230
Bsp1286I	GDGCHC	5 cut(s)	117, 165, 361, 775, 1213
Bsp13I	TCCGGA	2 cut(s)	48, 221
Bsp143I	GATC	5 cut(s)	126, 418, 465, 896, 1493
Bsp19I	CCATGG	1 cut(s)	700
BspANI	GGCC	2 cut(s)	180, 230
BspCNI	CTCAG	2 cut(s)	394, 1405
BspEI	TCCGGA	2 cut(s)	48, 221
BspFNI	CGCG	1 cut(s)	329
BspLI	GGNNCC	3 cut(s)	44, 239, 420
BspMI	ACCTGC	2 cut(s)	1599, 1610
BspPI	GGATC	3 cut(s)	413, 426, 473
BspT107I	GGYRCC	1 cut(s)	237
BsrDI	GCAATG	1 cut(s)	1636
BsrFI	RCCGGY	1 cut(s)	176
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	6 cut(s)	241, 353, 700, 1042, 1105, 1482
BssMI	GATC	5 cut(s)	126, 418, 465, 896, 1493
BssNI	GRCGYC	1 cut(s)	238
BssT1I	CCWWGG	1 cut(s)	700
Bst2UI	CCWGG	2 cut(s)	167, 1484
Bst4CI	ACNGT	7 cut(s)	291, 569, 920, 983, 1043, 1106, 1644
Bst6I	CTCTTC	1 cut(s)	90
BstACI	GRCGYC	1 cut(s)	238
BstBAI	YACGTR	2 cut(s)	839, 890
BstC8I	GCNNGC	4 cut(s)	87, 174, 178, 775
BstDEI	CTNAG	2 cut(s)	381, 1413
BstDSI	CCRYGG	3 cut(s)	700, 1042, 1105
BstF5I	GGATG	4 cut(s)	697, 1261, 1414, 1522
BstFNI	CGCG	1 cut(s)	329
BstH2I	RGCGCY	1 cut(s)	241
BstHHI	GCGC	3 cut(s)	240, 352, 712
BstKTI	GATC	5 cut(s)	129, 421, 468, 899, 1496
BstMBI	GATC	5 cut(s)	126, 418, 465, 896, 1493
BstNI	CCWGG	2 cut(s)	167, 1484
BstSCI	CCNGG	4 cut(s)	165, 241, 352, 1482
BstUI	CGCG	1 cut(s)	329
BstV1I	GCAGC	6 cut(s)	301, 307, 317, 380, 451, 1369
BstV2I	GAAGAC	3 cut(s)	285, 1011, 1197
BstX2I	RGATCY	2 cut(s)	418, 1493
BstYI	RGATCY	2 cut(s)	418, 1493
BsuI	GTATCC	1 cut(s)	874
BsuRI	GGCC	2 cut(s)	180, 230
BtgI	CCRYGG	3 cut(s)	700, 1042, 1105
BtrI	CACGTC	1 cut(s)	1214
BtsCI	GGATG	4 cut(s)	697, 1261, 1414, 1522
BtsI	GCAGTG	1 cut(s)	850
BtsIMutI	CAGTG	1 cut(s)	850
BveI	ACCTGC	2 cut(s)	1599, 1610
Cac8I	GCNNGC	4 cut(s)	87, 174, 178, 775
CaiI	CAGNNNCTG	1 cut(s)	812
CfoI	GCGC	3 cut(s)	240, 352, 712
Cfr10I	RCCGGY	1 cut(s)	176
CseI	GACGC	1 cut(s)	1331
CviAII	CATG	8 cut(s)	399, 701, 731, 794, 857, 1283, 1312, 1421
DdeI	CTNAG	2 cut(s)	381, 1413
DinI	GGCGCC	1 cut(s)	239
DpnI	GATC	5 cut(s)	128, 420, 467, 898, 1495
DpnII	GATC	5 cut(s)	126, 418, 465, 896, 1493
EaeI	YGGCCR	1 cut(s)	228
Eam1104I	CTCTTC	1 cut(s)	90
EarI	CTCTTC	1 cut(s)	90
EciI	GGCGGA	2 cut(s)	89, 1344
Ecl136II	GAGCTC	2 cut(s)	115, 163
Eco130I	CCWWGG	1 cut(s)	700
Eco24I	GRGCYC	3 cut(s)	117, 165, 361
Eco53kI	GAGCTC	2 cut(s)	115, 163
Eco57I	CTGAAG	2 cut(s)	834, 1635
EcoICRI	GAGCTC	2 cut(s)	115, 163
EcoRII	CCWGG	2 cut(s)	165, 1482
EcoT14I	CCWWGG	1 cut(s)	700
EcoT22I	ATGCAT	2 cut(s)	400, 1636
EcoT38I	GRGCYC	3 cut(s)	117, 165, 361
EgeI	GGCGCC	1 cut(s)	239
EheI	GGCGCC	1 cut(s)	239
ErhI	CCWWGG	1 cut(s)	700
FaeI	CATG	8 cut(s)	402, 704, 734, 797, 860, 1286, 1315, 1424
FalI	AAGNNNNNCTT	4 cut(s)	254, 286, 1496, 1528
FaqI	GGGAC	1 cut(s)	170
FatI	CATG	8 cut(s)	398, 700, 730, 793, 856, 1282, 1311, 1420
FokI	GGATG	4 cut(s)	704, 1268, 1421, 1529
FriOI	GRGCYC	3 cut(s)	117, 165, 361
FspBI	CTAG	1 cut(s)	1530
GlaI	GCGC	3 cut(s)	239, 351, 711
GsuI	CTGGAG	1 cut(s)	149
HaeII	RGCGCY	1 cut(s)	241
HaeIII	GGCC	2 cut(s)	180, 230
HapII	CCGG	6 cut(s)	40, 49, 177, 222, 243, 353
HgaI	GACGC	1 cut(s)	1331
HhaI	GCGC	3 cut(s)	240, 352, 712
Hin1I	GRCGYC	1 cut(s)	238
Hin1II	CATG	8 cut(s)	402, 704, 734, 797, 860, 1286, 1315, 1424
Hin6I	GCGC	3 cut(s)	238, 350, 710
HinP1I	GCGC	3 cut(s)	238, 350, 710
HindIII	AAGCTT	1 cut(s)	407
HpaII	CCGG	6 cut(s)	40, 49, 177, 222, 243, 353
HphI	GGTGA	4 cut(s)	265, 830, 1169, 1634
Hpy188III	TCNNGA	4 cut(s)	49, 222, 469, 484
Hpy99I	CGWCG	6 cut(s)	113, 558, 1218, 1347, 1587, 1644
HpyAV	CCTTC	8 cut(s)	20, 64, 280, 325, 541, 809, 1444, 1543
HpyCH4III	ACNGT	7 cut(s)	291, 569, 920, 983, 1043, 1106, 1644
HpyCH4IV	ACGT	7 cut(s)	63, 553, 763, 838, 889, 1213, 1441
HpyCH4V	TGCA	6 cut(s)	398, 1402, 1420, 1469, 1603, 1634
HpyF3I	CTNAG	2 cut(s)	381, 1413
HpySE526I	ACGT	7 cut(s)	63, 553, 763, 838, 889, 1213, 1441
Hsp92I	GRCGYC	1 cut(s)	238
Hsp92II	CATG	8 cut(s)	402, 704, 734, 797, 860, 1286, 1315, 1424
HspAI	GCGC	3 cut(s)	238, 350, 710
KasI	GGCGCC	1 cut(s)	237
Kpn2I	TCCGGA	2 cut(s)	48, 221
KroI	GCCGGC	1 cut(s)	176
KroNI	GCCGGC	1 cut(s)	178
Kzo9I	GATC	5 cut(s)	126, 418, 465, 896, 1493
LmnI	GCTCC	6 cut(s)	42, 76, 120, 152, 168, 169
Lsp1109I	GCAGC	6 cut(s)	301, 307, 317, 380, 451, 1369
MaeI	CTAG	1 cut(s)	1530
MaeII	ACGT	7 cut(s)	63, 553, 763, 838, 889, 1213, 1441
MaeIII	GTNAC	2 cut(s)	539, 890
MalI	GATC	5 cut(s)	128, 420, 467, 898, 1495
MboI	GATC	5 cut(s)	126, 418, 465, 896, 1493
MflI	RGATCY	2 cut(s)	418, 1493
MhlI	GDGCHC	5 cut(s)	117, 165, 361, 775, 1213
MluCI	AATT	4 cut(s)	256, 821, 1288, 1507
Mly113I	GGCGCC	1 cut(s)	238
MmeI	TCCRAC	1 cut(s)	536
Mph1103I	ATGCAT	2 cut(s)	400, 1636
MroI	TCCGGA	2 cut(s)	48, 221
MroNI	GCCGGC	1 cut(s)	176
MroXI	GAANNNNTTC	1 cut(s)	1114
MseI	TTAA	6 cut(s)	435, 672, 735, 924, 987, 1524
MslI	CAYNNNNRTG	2 cut(s)	717, 906
MspA1I	CMGCKG	3 cut(s)	314, 320, 623
MspI	CCGG	6 cut(s)	40, 49, 177, 222, 243, 353
MspR9I	CCNGG	4 cut(s)	167, 243, 354, 1484
Mva1269I	GAATGC	6 cut(s)	676, 865, 991, 1051, 1114, 1177
MvaI	CCWGG	2 cut(s)	167, 1484
MvnI	CGCG	1 cut(s)	329
NaeI	GCCGGC	1 cut(s)	178
NarI	GGCGCC	1 cut(s)	238
NciI	CCSGG	2 cut(s)	243, 354
NcoI	CCATGG	1 cut(s)	700
NdeII	GATC	5 cut(s)	126, 418, 465, 896, 1493
NgoMIV	GCCGGC	1 cut(s)	176
NlaIII	CATG	8 cut(s)	402, 704, 734, 797, 860, 1286, 1315, 1424
NlaIV	GGNNCC	3 cut(s)	44, 239, 420
NmeAIII	GCCGAG	1 cut(s)	1366
NmuCI	GTSAC	1 cut(s)	539
NsiI	ATGCAT	2 cut(s)	400, 1636
PaqCI	CACCTGC	1 cut(s)	1610
PctI	GAATGC	6 cut(s)	676, 865, 991, 1051, 1114, 1177
PdiI	GCCGGC	1 cut(s)	178
PdmI	GAANNNNTTC	1 cut(s)	1114
PfoI	TCCNGGA	1 cut(s)	165
PluTI	GGCGCC	1 cut(s)	241
Ppu21I	YACGTR	2 cut(s)	839, 890
Psp124BI	GAGCTC	2 cut(s)	117, 165
Psp1406I	AACGTT	1 cut(s)	1441
Psp6I	CCWGG	2 cut(s)	165, 1482
PspGI	CCWGG	2 cut(s)	165, 1482
PspN4I	GGNNCC	3 cut(s)	44, 239, 420
PstNI	CAGNNNCTG	1 cut(s)	812
PsuI	RGATCY	2 cut(s)	418, 1493
PvuII	CAGCTG	1 cut(s)	623
RseI	CAYNNNNRTG	2 cut(s)	717, 906
SacI	GAGCTC	2 cut(s)	117, 165
SaqAI	TTAA	6 cut(s)	435, 672, 735, 924, 987, 1524
Sau3AI	GATC	5 cut(s)	126, 418, 465, 896, 1493
ScrFI	CCNGG	4 cut(s)	167, 243, 354, 1484
SduI	GDGCHC	5 cut(s)	117, 165, 361, 775, 1213
SfoI	GGCGCC	1 cut(s)	239
SmiMI	CAYNNNNRTG	2 cut(s)	717, 906
SpeI	ACTAGT	1 cut(s)	1529
Sse9I	AATT	4 cut(s)	256, 821, 1288, 1507
SspDI	GGCGCC	1 cut(s)	237
SspMI	CTAG	1 cut(s)	1530
SstI	GAGCTC	2 cut(s)	117, 165
StyD4I	CCNGG	4 cut(s)	165, 241, 352, 1482
StyI	CCWWGG	1 cut(s)	700
TaaI	ACNGT	7 cut(s)	291, 569, 920, 983, 1043, 1106, 1644
TaiI	ACGT	7 cut(s)	66, 556, 766, 841, 892, 1216, 1444
TaqI	TCGA	8 cut(s)	108, 715, 1027, 1090, 1153, 1216, 1321, 1639
TasI	AATT	4 cut(s)	256, 821, 1288, 1507
TauI	GCSGC	5 cut(s)	92, 249, 311, 314, 320
Tru1I	TTAA	6 cut(s)	435, 672, 735, 924, 987, 1524
Tru9I	TTAA	6 cut(s)	435, 672, 735, 924, 987, 1524
TscAI	CASTG	1 cut(s)	850
TseFI	GTSAC	1 cut(s)	539
TseI	GCWGC	6 cut(s)	305, 314, 320, 368, 439, 1382
Tsp45I	GTSAC	1 cut(s)	539
TspDTI	ATGAA	6 cut(s)	708, 771, 1146, 1299, 1341, 1437
TspGWI	ACGGA	2 cut(s)	146, 1571
TspRI	CASTG	1 cut(s)	850
XapI	RAATTY	2 cut(s)	1288, 1507
XmnI	GAANNNNTTC	1 cut(s)	1114
XspI	CTAG	1 cut(s)	1530
Zsp2I	ATGCAT	2 cut(s)	400, 1636

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.