Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0006065

Physical Location & Seq

Genomic Coordinates Forward (+)

35938901 .. 35941586

Gene Structure 2686 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G015600.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1641 bp

ATGGCGTTGAAGTTTCTGAACAAGAAGGGGTGGCACACCGGGAGCCTCCGGAACATAGAGAACGTGTGGAAGGCGGAGCAGAAGCACGCCGCCGAAGAGAAGAAGCTCGACGAGCTCCGCAAACAGATCGTCGAAGAAAGAGAACGCTCCGAGTTCCGTGAGCTCCAGGAGCAAGCCGGCCTCGTCCCCAAGCAAGAGCGATTGGAGTTCCTCTACGATTCCGGATTGGCCGTAGGGCGCCCCGGCGGCTCTGATAATTTCAAGGAAGCATTCACTAAAGCCGAAGAACCGCCTTCTTCCTCCTCCGCCGCCGCCGCCACCGCGAAGGAGGCATCATCGTCTGCGCCGGGGGCTCTGTTCGCAGCAGACAAGCCTCAGTCTGCCAATGATGCATGGAGGAAGCTTCACTCGGATCCTTTGCTTATGATTAAGCAGCGAGAGCAGGAAGCCCTCTCTCGGATTAAGAACAATCCTGTCCAGATGGCTATGATTCGCAAACAAGTTGAAGCAAAGAAGCCCAAAGAAAAAGAGCGTGACAAGAAGGAACGTCGGAAGAAACACCGTAAAGATTCTTCAAAGCATCGGAAGCAAACATCTGATTCAGAAGATGACACAGCTGATATGGAAAGGAGGAAGAATAGTCATCGTAAGGCATCAAAATATGTTAAGCATTCAGAATCAGAGGATGAACCCCATGAAAAAGCGCATCGAAGTGAGAAGAACCATGTTAAGCAGTCAAATGTTGAAGATGAATCTCGTTATAGAGCACGCCAAAGTGGGAAAACCCATGCCAAGTATTCAGATTCTGAAGATGAATTGCGTAGCAAAGCACGTAGCAGTGATAAAAATCATGTTAGGCATTCAGATTTGGAGGATACATCACGTAACAGATCACATCGTAGTGAGAAGAACCGTGTTAAGTATTCAGATTCAGAAGATGAGCTACATAACAGAGGGCGTGGAAGTGAGAAAAACCGTGTTAAGCATTCAGATTCAGAAGACAAGCTACACAACAGAGGTCGAAGTGAGAACAACCGTGGTAAGCATTCAGATTCAGAAGATGAGCTACATAACAGAAGAAGTCGAAGTGAGAGAAACCGTGGAAAGCATTCAGATTCAGAAGATGAACTACATAACAGAAGAGGTCGAGGTGAGAAGAACCGTGTCAAGCATTCAGATTCAGAAGACGGGCTACATAACAGAGCACGTCGAAGTGAGAAAGATGATGCTATAACAAGGGGAAAAGAGGACGGTCAAGGTATTGTCAAGGGAAAACATGAAAATTCAGAACAAGAAAACTATTCCATGAGAGGTCGAAATGAATCCCACAACAAGCGTCGGTTTGTTCCGCCTAAACTTTCAGAAGAAGAAAGAGCTGCTCGGCTAAAGGAGATGCAGATGGATGCTGAGTTGCATGAAGAACAAAGATGGAAACGTTTGAAGAAGGCTGACGAGAATGATGCACGGGAAGCTACCCAGGCTATCAGATCTGGCGGTAAGAATTTCTTGGATGCGGTTAAAACTAGTGTTTATGGTTCTGGGAAGGGAGGAAGCTCAACAATAGAGGAGAGTGTCCGTCGCCGTACCCATTATTTGCAGGGCAGGTCTGAAGCAGGTGAAAGCAATGCATTTCGACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CWC25 Cir_N

Protein Analysis

546

Amino Acids

63.14

Weight (kDa)

9.66

Isoelectric Point (pI)

69.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cir_N	PF10197	11 - 46	5e-14	N-terminal domain of CBF1 interacting co-repressor CIR
CWC25	PF12542	64 - 158	1.4e-14	Pre-mRNA splicing factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44195 AT2G44200
fragaria_vesca	FvH4_4g13451
malus_domestica	MD04G1014200.v1.1
prunus_persica	Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G162300_v2.0.a1
pyrus_communis	pycom04g01100
rosa_chinensis	RchiOBHm_Chr4g0414011
rosa_laevigata	RLG00000008185
rosa_multiflora	Rmu_sc0003264.1_g000022 Rmu_sc0006428.1_g000008
rosa_roxburghii	Rroxscaffold_5G00357930
rosa_rugosa	Rorug04G0125000
rosa_samantha	Rh4AG183500 Rh4BG183000 Rh4CG195500 Rh4DG178800
rosa_wichuraiana	Rw4G015600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1604
Acc36I	ACCTGC	2 cut(s)	1593, 1604
AccB1I	GGYRCC	1 cut(s)	237
AccII	CGCG	1 cut(s)	323
AccIII	TCCGGA	2 cut(s)	48, 221
AclI	AACGTT	1 cut(s)	1435
AclWI	GGATC	2 cut(s)	407, 420
AcoI	YGGCCR	1 cut(s)	228
AcsI	RAATTY	2 cut(s)	1282, 1501
AcuI	CTGAAG	2 cut(s)	828, 1629
AcyI	GRCGYC	1 cut(s)	238
AfaI	GTAC	1 cut(s)	1585
AfiI	CCNNNNNNNGG	1 cut(s)	456
AflIII	ACRYGT	1 cut(s)	63
AgsI	TTSAA	6 cut(s)	10, 262, 506, 576, 746, 1441
AhlI	ACTAGT	1 cut(s)	1523
AjiI	CACGTC	1 cut(s)	1208
AjnI	CCWGG	2 cut(s)	165, 1476
Alw21I	GWGCWC	4 cut(s)	117, 165, 769, 1207
AlwI	GGATC	2 cut(s)	407, 420
AlwNI	CAGNNNCTG	1 cut(s)	806
Aor13HI	TCCGGA	2 cut(s)	48, 221
AoxI	GGCC	2 cut(s)	178, 228
ApeKI	GCWGC	3 cut(s)	362, 433, 1376
ApoI	RAATTY	2 cut(s)	1282, 1501
ArsI	GACNNNNNNTTYG	2 cut(s)	114, 146
Asp700I	GAANNNNTTC	1 cut(s)	1108
AspLEI	GCGC	3 cut(s)	240, 346, 706
AsuC2I	CCSGG	3 cut(s)	40, 243, 348
AsuHPI	GGTGA	2 cut(s)	1163, 1628
BamHI	GGATCC	1 cut(s)	412
BanI	GGYRCC	1 cut(s)	237
BanII	GRGCYC	3 cut(s)	117, 165, 355
BbsI	GAAGAC	2 cut(s)	1005, 1191
Bbv12I	GWGCWC	4 cut(s)	117, 165, 769, 1207
BbvI	GCAGC	3 cut(s)	374, 445, 1363
BccI	CCATC	3 cut(s)	475, 1393, 1422
BceAI	ACGGC	2 cut(s)	215, 1566
BcgI	CGANNNNNNTGC	4 cut(s)	109, 143, 1442, 1476
BciT130I	CCWGG	2 cut(s)	167, 1478
BciVI	GTATCC	1 cut(s)	868
BcnI	CCSGG	3 cut(s)	40, 243, 348
BcuI	ACTAGT	1 cut(s)	1523
BfaI	CTAG	1 cut(s)	1524
BfoI	RGCGCY	1 cut(s)	241
BfuAI	ACCTGC	2 cut(s)	1593, 1604
BfuI	GTATCC	1 cut(s)	868
BglI	GCCNNNNNGGC	1 cut(s)	246
BglII	AGATCT	1 cut(s)	1487
BisI	GCNGC	8 cut(s)	90, 247, 309, 312, 315, 363, 434, 1377
BlsI	GCNGC	8 cut(s)	91, 248, 310, 313, 316, 364, 435, 1378
Bme1390I	CCNGG	5 cut(s)	40, 167, 243, 348, 1478
BmgBI	CACGTC	1 cut(s)	1208
BmiI	GGNNCC	3 cut(s)	44, 239, 414
BmrFI	CCNGG	5 cut(s)	40, 167, 243, 348, 1478
BpiI	GAAGAC	2 cut(s)	1005, 1191
BpmI	CTGGAG	1 cut(s)	149
BpuMI	CCSGG	3 cut(s)	40, 243, 348
BsaAI	YACGTR	2 cut(s)	833, 884
BsaBI	GATNNNNATC	1 cut(s)	846
BsaHI	GRCGYC	1 cut(s)	238
BsaJI	CCNNGG	5 cut(s)	241, 347, 1036, 1099, 1476
BsaWI	WCCGGW	2 cut(s)	48, 221
Bsc4I	CCNNNNNNNGG	1 cut(s)	456
Bse118I	RCCGGY	1 cut(s)	176
Bse3DI	GCAATG	1 cut(s)	1630
Bse8I	GATNNNNATC	1 cut(s)	846
BseAI	TCCGGA	2 cut(s)	48, 221
BseBI	CCWGG	2 cut(s)	167, 1478
BseDI	CCNNGG	5 cut(s)	241, 347, 1036, 1099, 1476
BseGI	GGATG	3 cut(s)	691, 1408, 1516
BseJI	GATNNNNATC	1 cut(s)	846
BseLI	CCNNNNNNNGG	1 cut(s)	456
BseMI	GCAATG	1 cut(s)	1630
BseMII	CTCAG	2 cut(s)	389, 1398
BseRI	GAGGAG	2 cut(s)	292, 1580
BseXI	GCAGC	3 cut(s)	374, 445, 1363
Bsh1236I	CGCG	1 cut(s)	323
BshFI	GGCC	2 cut(s)	180, 230
BshNI	GGYRCC	1 cut(s)	237
BsiHKAI	GWGCWC	4 cut(s)	117, 165, 769, 1207
BsiSI	CCGG	6 cut(s)	39, 49, 177, 222, 243, 347
BslFI	GGGAC	1 cut(s)	170
BslI	CCNNNNNNNGG	1 cut(s)	456
BsmFI	GGGAC	1 cut(s)	170
BsmI	GAATGC	7 cut(s)	269, 670, 859, 985, 1045, 1108, 1171
BsnI	GGCC	2 cut(s)	180, 230
Bsp1286I	GDGCHC	5 cut(s)	117, 165, 355, 769, 1207
Bsp13I	TCCGGA	2 cut(s)	48, 221
Bsp143I	GATC	4 cut(s)	126, 412, 890, 1487
BspANI	GGCC	2 cut(s)	180, 230
BspCNI	CTCAG	2 cut(s)	388, 1399
BspEI	TCCGGA	2 cut(s)	48, 221
BspFNI	CGCG	1 cut(s)	323
BspLI	GGNNCC	3 cut(s)	44, 239, 414
BspMI	ACCTGC	2 cut(s)	1593, 1604
BspPI	GGATC	2 cut(s)	407, 420
BspT107I	GGYRCC	1 cut(s)	237
BsrDI	GCAATG	1 cut(s)	1630
BsrFI	RCCGGY	1 cut(s)	176
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	5 cut(s)	241, 347, 1036, 1099, 1476
BssMI	GATC	4 cut(s)	126, 412, 890, 1487
BssNI	GRCGYC	1 cut(s)	238
Bst2UI	CCWGG	2 cut(s)	167, 1478
Bst4CI	ACNGT	8 cut(s)	563, 914, 977, 1037, 1100, 1163, 1253, 1638
Bst6I	CTCTTC	2 cut(s)	90, 1135
BstACI	GRCGYC	1 cut(s)	238
BstBAI	YACGTR	2 cut(s)	833, 884
BstC8I	GCNNGC	4 cut(s)	87, 174, 178, 769
BstDEI	CTNAG	2 cut(s)	375, 1407
BstDSI	CCRYGG	2 cut(s)	1036, 1099
BstF5I	GGATG	3 cut(s)	691, 1408, 1516
BstFNI	CGCG	1 cut(s)	323
BstH2I	RGCGCY	1 cut(s)	241
BstHHI	GCGC	3 cut(s)	240, 346, 706
BstKTI	GATC	4 cut(s)	129, 415, 893, 1490
BstMBI	GATC	4 cut(s)	126, 412, 890, 1487
BstNI	CCWGG	2 cut(s)	167, 1478
BstSCI	CCNGG	5 cut(s)	38, 165, 241, 346, 1476
BstUI	CGCG	1 cut(s)	323
BstV1I	GCAGC	3 cut(s)	374, 445, 1363
BstV2I	GAAGAC	2 cut(s)	1005, 1191
BstX2I	RGATCY	2 cut(s)	412, 1487
BstYI	RGATCY	2 cut(s)	412, 1487
BsuI	GTATCC	1 cut(s)	868
BsuRI	GGCC	2 cut(s)	180, 230
BtgI	CCRYGG	2 cut(s)	1036, 1099
BtrI	CACGTC	1 cut(s)	1208
BtsCI	GGATG	3 cut(s)	691, 1408, 1516
BtsI	GCAGTG	1 cut(s)	844
BtsIMutI	CAGTG	1 cut(s)	844
BveI	ACCTGC	2 cut(s)	1593, 1604
Cac8I	GCNNGC	4 cut(s)	87, 174, 178, 769
CaiI	CAGNNNCTG	1 cut(s)	806
CfoI	GCGC	3 cut(s)	240, 346, 706
Cfr10I	RCCGGY	1 cut(s)	176
CseI	GACGC	1 cut(s)	1325
Csp6I	GTAC	1 cut(s)	1584
CviAII	CATG	8 cut(s)	393, 695, 725, 788, 851, 1277, 1306, 1415
CviQI	GTAC	1 cut(s)	1584
DdeI	CTNAG	2 cut(s)	375, 1407
DinI	GGCGCC	1 cut(s)	239
DpnI	GATC	4 cut(s)	128, 414, 892, 1489
DpnII	GATC	4 cut(s)	126, 412, 890, 1487
EaeI	YGGCCR	1 cut(s)	228
Eam1104I	CTCTTC	2 cut(s)	90, 1135
EarI	CTCTTC	2 cut(s)	90, 1135
EciI	GGCGGA	3 cut(s)	89, 295, 1338
Ecl136II	GAGCTC	2 cut(s)	115, 163
Eco24I	GRGCYC	3 cut(s)	117, 165, 355
Eco53kI	GAGCTC	2 cut(s)	115, 163
Eco57I	CTGAAG	2 cut(s)	828, 1629
EcoICRI	GAGCTC	2 cut(s)	115, 163
EcoRII	CCWGG	2 cut(s)	165, 1476
EcoT22I	ATGCAT	2 cut(s)	394, 1630
EcoT38I	GRGCYC	3 cut(s)	117, 165, 355
EgeI	GGCGCC	1 cut(s)	239
EheI	GGCGCC	1 cut(s)	239
FaeI	CATG	8 cut(s)	396, 698, 728, 791, 854, 1280, 1309, 1418
FalI	AAGNNNNNCTT	2 cut(s)	1490, 1522
FaqI	GGGAC	1 cut(s)	170
FatI	CATG	8 cut(s)	392, 694, 724, 787, 850, 1276, 1305, 1414
Fnu4HI	GCNGC	8 cut(s)	90, 247, 309, 312, 315, 363, 434, 1377
FokI	GGATG	3 cut(s)	698, 1415, 1523
FriOI	GRGCYC	3 cut(s)	117, 165, 355
Fsp4HI	GCNGC	8 cut(s)	90, 247, 309, 312, 315, 363, 434, 1377
FspBI	CTAG	1 cut(s)	1524
GlaI	GCGC	3 cut(s)	239, 345, 705
GluI	GCNGC	8 cut(s)	90, 247, 309, 312, 315, 363, 434, 1377
GsuI	CTGGAG	1 cut(s)	149
HaeII	RGCGCY	1 cut(s)	241
HaeIII	GGCC	2 cut(s)	180, 230
HapII	CCGG	6 cut(s)	39, 49, 177, 222, 243, 347
HgaI	GACGC	1 cut(s)	1325
HhaI	GCGC	3 cut(s)	240, 346, 706
Hin1I	GRCGYC	1 cut(s)	238
Hin1II	CATG	8 cut(s)	396, 698, 728, 791, 854, 1280, 1309, 1418
Hin6I	GCGC	3 cut(s)	238, 344, 704
HinP1I	GCGC	3 cut(s)	238, 344, 704
HindIII	AAGCTT	1 cut(s)	401
HpaII	CCGG	6 cut(s)	39, 49, 177, 222, 243, 347
HphI	GGTGA	2 cut(s)	1163, 1628
Hpy188III	TCNNGA	3 cut(s)	49, 222, 478
Hpy99I	CGWCG	7 cut(s)	113, 134, 552, 1212, 1341, 1581, 1638
HpyAV	CCTTC	7 cut(s)	19, 64, 303, 319, 535, 1438, 1537
HpyCH4III	ACNGT	8 cut(s)	563, 914, 977, 1037, 1100, 1163, 1253, 1638
HpyCH4IV	ACGT	6 cut(s)	63, 547, 832, 883, 1207, 1435
HpyCH4V	TGCA	6 cut(s)	392, 1396, 1414, 1463, 1597, 1628
HpyF3I	CTNAG	2 cut(s)	375, 1407
HpySE526I	ACGT	6 cut(s)	63, 547, 832, 883, 1207, 1435
Hsp92I	GRCGYC	1 cut(s)	238
Hsp92II	CATG	8 cut(s)	396, 698, 728, 791, 854, 1280, 1309, 1418
HspAI	GCGC	3 cut(s)	238, 344, 704
KasI	GGCGCC	1 cut(s)	237
Kpn2I	TCCGGA	2 cut(s)	48, 221
KroI	GCCGGC	1 cut(s)	176
KroNI	GCCGGC	1 cut(s)	178
Kzo9I	GATC	4 cut(s)	126, 412, 890, 1487
LmnI	GCTCC	6 cut(s)	42, 76, 120, 152, 168, 169
Lsp1109I	GCAGC	3 cut(s)	374, 445, 1363
MaeI	CTAG	1 cut(s)	1524
MaeII	ACGT	6 cut(s)	63, 547, 832, 883, 1207, 1435
MaeIII	GTNAC	2 cut(s)	533, 884
MalI	GATC	4 cut(s)	128, 414, 892, 1489
MboI	GATC	4 cut(s)	126, 412, 890, 1487
MflI	RGATCY	2 cut(s)	412, 1487
MhlI	GDGCHC	5 cut(s)	117, 165, 355, 769, 1207
MluCI	AATT	4 cut(s)	256, 815, 1282, 1501
Mly113I	GGCGCC	1 cut(s)	238
MmeI	TCCRAC	1 cut(s)	530
Mph1103I	ATGCAT	2 cut(s)	394, 1630
MroI	TCCGGA	2 cut(s)	48, 221
MroNI	GCCGGC	1 cut(s)	176
MroXI	GAANNNNTTC	1 cut(s)	1108
MseI	TTAA	7 cut(s)	429, 462, 666, 729, 918, 981, 1518
MslI	CAYNNNNRTG	2 cut(s)	711, 900
MspA1I	CMGCKG	1 cut(s)	617
MspI	CCGG	6 cut(s)	39, 49, 177, 222, 243, 347
MspR9I	CCNGG	5 cut(s)	40, 167, 243, 348, 1478
Mva1269I	GAATGC	7 cut(s)	269, 670, 859, 985, 1045, 1108, 1171
MvaI	CCWGG	2 cut(s)	167, 1478
MvnI	CGCG	1 cut(s)	323
NaeI	GCCGGC	1 cut(s)	178
NarI	GGCGCC	1 cut(s)	238
NciI	CCSGG	3 cut(s)	40, 243, 348
NdeII	GATC	4 cut(s)	126, 412, 890, 1487
NgoMIV	GCCGGC	1 cut(s)	176
NlaIII	CATG	8 cut(s)	396, 698, 728, 791, 854, 1280, 1309, 1418
NlaIV	GGNNCC	3 cut(s)	44, 239, 414
NmeAIII	GCCGAG	1 cut(s)	1360
NmuCI	GTSAC	1 cut(s)	533
NsiI	ATGCAT	2 cut(s)	394, 1630
PaqCI	CACCTGC	1 cut(s)	1604
PctI	GAATGC	7 cut(s)	269, 670, 859, 985, 1045, 1108, 1171
PdiI	GCCGGC	1 cut(s)	178
PdmI	GAANNNNTTC	1 cut(s)	1108
PfoI	TCCNGGA	1 cut(s)	165
PkrI	GCNGC	8 cut(s)	91, 248, 310, 313, 316, 364, 435, 1378
PluTI	GGCGCC	1 cut(s)	241
Ppu21I	YACGTR	2 cut(s)	833, 884
Psp124BI	GAGCTC	2 cut(s)	117, 165
Psp1406I	AACGTT	1 cut(s)	1435
Psp6I	CCWGG	2 cut(s)	165, 1476
PspGI	CCWGG	2 cut(s)	165, 1476
PspN4I	GGNNCC	3 cut(s)	44, 239, 414
PstNI	CAGNNNCTG	1 cut(s)	806
PsuI	RGATCY	2 cut(s)	412, 1487
PvuII	CAGCTG	1 cut(s)	617
RsaI	GTAC	1 cut(s)	1585
RsaNI	GTAC	1 cut(s)	1584
RseI	CAYNNNNRTG	2 cut(s)	711, 900
SacI	GAGCTC	2 cut(s)	117, 165
SaqAI	TTAA	7 cut(s)	429, 462, 666, 729, 918, 981, 1518
SatI	GCNGC	8 cut(s)	90, 247, 309, 312, 315, 363, 434, 1377
Sau3AI	GATC	4 cut(s)	126, 412, 890, 1487
ScrFI	CCNGG	5 cut(s)	40, 167, 243, 348, 1478
SduI	GDGCHC	5 cut(s)	117, 165, 355, 769, 1207
SfoI	GGCGCC	1 cut(s)	239
SmiMI	CAYNNNNRTG	2 cut(s)	711, 900
SpeI	ACTAGT	1 cut(s)	1523
Sse9I	AATT	4 cut(s)	256, 815, 1282, 1501
SspDI	GGCGCC	1 cut(s)	237
SspMI	CTAG	1 cut(s)	1524
SstI	GAGCTC	2 cut(s)	117, 165
StyD4I	CCNGG	5 cut(s)	38, 165, 241, 346, 1476
TaaI	ACNGT	8 cut(s)	563, 914, 977, 1037, 1100, 1163, 1253, 1638
TaiI	ACGT	6 cut(s)	66, 550, 835, 886, 1210, 1438
TaqI	TCGA	9 cut(s)	108, 132, 709, 1021, 1084, 1147, 1210, 1315, 1633
TasI	AATT	4 cut(s)	256, 815, 1282, 1501
TauI	GCSGC	5 cut(s)	92, 249, 311, 314, 317
Tru1I	TTAA	7 cut(s)	429, 462, 666, 729, 918, 981, 1518
Tru9I	TTAA	7 cut(s)	429, 462, 666, 729, 918, 981, 1518
TscAI	CASTG	1 cut(s)	844
TseFI	GTSAC	1 cut(s)	533
TseI	GCWGC	3 cut(s)	362, 433, 1376
Tsp45I	GTSAC	1 cut(s)	533
TspDTI	ATGAA	8 cut(s)	702, 711, 765, 828, 1140, 1293, 1335, 1431
TspGWI	ACGGA	2 cut(s)	146, 1565
TspRI	CASTG	1 cut(s)	844
XapI	RAATTY	2 cut(s)	1282, 1501
XmnI	GAANNNNTTC	1 cut(s)	1108
XspI	CTAG	1 cut(s)	1524
Zsp2I	ATGCAT	2 cut(s)	394, 1630

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.