Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0006065

Physical Location & Seq

Genomic Coordinates Forward (+)

41806533 .. 41809733

Gene Structure 3201 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG195500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1581 bp

ATGGCGTTGAAGTTTCTGAACAAGAAGGGGTGGCACACCGGGAGCCTCCGGAACATAGAGAACGTGTGGAAGGCGGAGCAGAAGCACGCCGCCGAAGAGAAGAAGCTCGACGAGCTCCGCAAACAGATCGTCGAAGAAAGAGAACGATCCGAGTTCCGTGAGCTCCAGGAGCAAGCCGGCCTCGTCCCCAAGCAAGAACGATTGGAGTTCCTCTACGATTCCGGATTGGCCGTAGGGCGCCCTGGCGGCTCTGATAATTTCAAGGAAGCCTTCACCAAAGCCGAAGAACCGTCTTCTTCCTCCGCCGCTGCCGCTGCCACCGCTAAGGAGGCATCATCGGCTGCGCCGGGGGCTCTGTTCGCAGCAGACAAGCCTCAGTCTGCCAATGATGCATGGAGGAAGCTTCACTCGGATCCTTTGCTTATGATTAAGCAGCGAGAGCAGGAAGCCCTCTCTCGGATCAAGAACAATCCTGTCCAGATGGCTATGATTCGCAAACAAGTTGAAGCAAAGAAGCCTAAGGAAAAAGAGCGTGACAAGAAGGAACATCGGAAGAAACACCGTAAAGATTCTTCAAAGCACCGGAAGCAAACGTCTGATTCAGAAGATGACACAGCTGATATGGAAAGGAGGAAGAATAGTCATCATAAGGCATCAAAATATGTTAAGCATTCAGAATCAGAGGATGAACCACATGGAAAAGCACATCGAACTGAGAAGAACCATGTTAGGCAGTCAAATGTTGAAGATGAATCACGTTATAGAGCACGCCAAAGTGGGAAAACCCATGCTAAGTATTCAGATTCTGAAGATGAATTGCGTAGCAAAGCACGTAGTAGTGGTAAAAACCATGTTAGGCATTCAGATTTGGAGGATACATCACATAACAGAGCACGTCGAAGTGAGAAGAACCGTGTTAAGCATTCAGATTCAGAAGATGGGCTACATAACAGAAGAAGTCGAAGTGAGAAGAACCGTGGCAAGCATTCAGATTCAGAAGATGAGCTACATAACAGAAGAAGTCGAAGTGAGAGAAACCGTGGTAAGCATTCAGATTCAGAAGATGAACTACATAACAGAACAAGTCGAAGTGAGAAGAAGCATGTCAAGCATTCAGATTCAGAAGACGGGCTACATAACAGAGCACGTCGAAGTGAGACAGATCATGCCATAACAAGGGCAAAAGACGACGGTCAAGGTATTGTCAAGGGAAGACATGAAAATTCAGAACAAGAAAACTATTCAATGAGAAGTCGAAATGAATCCCACAACAAGCGTCGGTTTGTTCCACCTAAACTTTCAGAAGAAGAAAGAGCTGCTAGGCTAAAGGAGATGCAGATGGATGCTGAGTTGCATGAAGAACAAAGATGGAAACGTTTGAAGAAGGCTGACGAGAATGATGCACGAGAAGCTACCCAAGCTATCAGATCTGGCGGTAAGAATTTCTTGGATGCTGTTAAAACTAGTGTTTATGGTTCTGGGAAGGGAGGAAGCTCAACAATAGAGGAGAGTGTCCGTCGCAGAACACATTATTTGCAGGGCAGGTCTGAAGCAGGTGAAAGCAATGCATTTCGACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CWC25 Cir_N

Protein Analysis

526

Amino Acids

60.68

Weight (kDa)

9.71

Isoelectric Point (pI)

73.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cir_N	PF10197	11 - 46	4.8e-14	N-terminal domain of CBF1 interacting co-repressor CIR
CWC25	PF12542	64 - 158	6.1e-15	Pre-mRNA splicing factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44195 AT2G44200
fragaria_vesca	FvH4_4g13451
malus_domestica	MD04G1014200.v1.1
prunus_persica	Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G162300_v2.0.a1
pyrus_communis	pycom04g01100
rosa_chinensis	RchiOBHm_Chr4g0414011
rosa_laevigata	RLG00000008185
rosa_multiflora	Rmu_sc0003264.1_g000022 Rmu_sc0006428.1_g000008
rosa_roxburghii	Rroxscaffold_5G00357930
rosa_rugosa	Rorug04G0125000
rosa_samantha	Rh4AG183500 Rh4BG183000 Rh4CG195500 Rh4DG178800
rosa_wichuraiana	Rw4G015600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1544
Acc36I	ACCTGC	2 cut(s)	1533, 1544
AccB1I	GGYRCC	1 cut(s)	237
AccIII	TCCGGA	2 cut(s)	48, 221
AciI	CCGC	9 cut(s)	74, 90, 118, 246, 303, 306, 312, 321, 1434
AclI	AACGTT	1 cut(s)	1375
AclWI	GGATC	4 cut(s)	141, 407, 420, 467
AcoI	YGGCCR	1 cut(s)	228
AcsI	RAATTY	2 cut(s)	1222, 1441
AcuI	CTGAAG	2 cut(s)	828, 1569
AcyI	GRCGYC	1 cut(s)	238
AfiI	CCNNNNNNNGG	2 cut(s)	456, 1176
AflIII	ACRYGT	1 cut(s)	63
AgsI	TTSAA	7 cut(s)	10, 262, 506, 576, 746, 1245, 1381
AhlI	ACTAGT	1 cut(s)	1463
AjiI	CACGTC	2 cut(s)	896, 1148
AjnI	CCWGG	2 cut(s)	165, 241
Alw21I	GWGCWC	5 cut(s)	117, 165, 769, 895, 1147
Alw26I	GTCTC	1 cut(s)	1151
AlwI	GGATC	4 cut(s)	141, 407, 420, 467
AlwNI	CAGNNNCTG	1 cut(s)	806
Aor13HI	TCCGGA	2 cut(s)	48, 221
AoxI	GGCC	2 cut(s)	178, 228
ApeKI	GCWGC	6 cut(s)	308, 314, 341, 362, 433, 1316
ApoI	RAATTY	2 cut(s)	1222, 1441
ArsI	GACNNNNNNTTYG	2 cut(s)	114, 146
AspLEI	GCGC	2 cut(s)	240, 346
AsuC2I	CCSGG	2 cut(s)	40, 348
AsuHPI	GGTGA	2 cut(s)	265, 1568
AxyI	CCTNAGG	1 cut(s)	519
BamHI	GGATCC	1 cut(s)	412
BanI	GGYRCC	1 cut(s)	237
BanII	GRGCYC	3 cut(s)	117, 165, 355
BauI	CACGAG	1 cut(s)	1404
BbsI	GAAGAC	3 cut(s)	285, 1131, 1219
Bbv12I	GWGCWC	5 cut(s)	117, 165, 769, 895, 1147
BbvI	GCAGC	6 cut(s)	295, 301, 328, 374, 445, 1303
BccI	CCATC	4 cut(s)	475, 932, 1333, 1362
BceAI	ACGGC	1 cut(s)	215
BcgI	CGANNNNNNTGC	4 cut(s)	109, 143, 1382, 1416
BciT130I	CCWGG	2 cut(s)	167, 243
BciVI	GTATCC	1 cut(s)	868
BcnI	CCSGG	2 cut(s)	40, 348
BcoDI	GTCTC	1 cut(s)	1151
BcuI	ACTAGT	1 cut(s)	1463
BfaI	CTAG	2 cut(s)	1320, 1464
BfoI	RGCGCY	1 cut(s)	241
BfuAI	ACCTGC	2 cut(s)	1533, 1544
BfuI	GTATCC	1 cut(s)	868
BglI	GCCNNNNNGGC	1 cut(s)	246
BglII	AGATCT	1 cut(s)	1427
Bme1390I	CCNGG	4 cut(s)	40, 167, 243, 348
BmgBI	CACGTC	2 cut(s)	896, 1148
BmiI	GGNNCC	3 cut(s)	44, 239, 414
BmrFI	CCNGG	4 cut(s)	40, 167, 243, 348
BmsI	GCATC	7 cut(s)	341, 379, 662, 1323, 1333, 1390, 1441
BoxI	GACNNNNGTC	1 cut(s)	1191
BpiI	GAAGAC	3 cut(s)	285, 1131, 1219
BpmI	CTGGAG	1 cut(s)	149
Bpu10I	CCTNAGC	1 cut(s)	324
BpuMI	CCSGG	2 cut(s)	40, 348
BsaAI	YACGTR	1 cut(s)	833
BsaHI	GRCGYC	1 cut(s)	238
BsaJI	CCNNGG	4 cut(s)	241, 347, 976, 1039
BsaWI	WCCGGW	3 cut(s)	48, 221, 582
Bsc4I	CCNNNNNNNGG	2 cut(s)	456, 1176
Bse118I	RCCGGY	1 cut(s)	176
Bse21I	CCTNAGG	1 cut(s)	519
Bse3DI	GCAATG	1 cut(s)	1570
BseAI	TCCGGA	2 cut(s)	48, 221
BseBI	CCWGG	2 cut(s)	167, 243
BseDI	CCNNGG	4 cut(s)	241, 347, 976, 1039
BseGI	GGATG	3 cut(s)	691, 1348, 1456
BseLI	CCNNNNNNNGG	2 cut(s)	456, 1176
BseMI	GCAATG	1 cut(s)	1570
BseMII	CTCAG	3 cut(s)	389, 705, 1338
BseRI	GAGGAG	1 cut(s)	1520
BseXI	GCAGC	6 cut(s)	295, 301, 328, 374, 445, 1303
BshFI	GGCC	2 cut(s)	180, 230
BshNI	GGYRCC	1 cut(s)	237
BsiHKAI	GWGCWC	5 cut(s)	117, 165, 769, 895, 1147
BsiSI	CCGG	6 cut(s)	39, 49, 177, 222, 347, 583
BslFI	GGGAC	1 cut(s)	170
BslI	CCNNNNNNNGG	2 cut(s)	456, 1176
BsmAI	GTCTC	1 cut(s)	1151
BsmFI	GGGAC	1 cut(s)	170
BsmI	GAATGC	6 cut(s)	670, 859, 922, 985, 1048, 1111
BsnI	GGCC	2 cut(s)	180, 230
Bsp1286I	GDGCHC	6 cut(s)	117, 165, 355, 769, 895, 1147
Bsp13I	TCCGGA	2 cut(s)	48, 221
Bsp143I	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
BspACI	CCGC	9 cut(s)	74, 90, 118, 246, 303, 306, 312, 321, 1434
BspANI	GGCC	2 cut(s)	180, 230
BspCNI	CTCAG	3 cut(s)	388, 706, 1339
BspEI	TCCGGA	2 cut(s)	48, 221
BspLI	GGNNCC	3 cut(s)	44, 239, 414
BspMI	ACCTGC	2 cut(s)	1533, 1544
BspPI	GGATC	4 cut(s)	141, 407, 420, 467
BspT107I	GGYRCC	1 cut(s)	237
BsrDI	GCAATG	1 cut(s)	1570
BsrFI	RCCGGY	1 cut(s)	176
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	4 cut(s)	241, 347, 976, 1039
BssMI	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
BssNI	GRCGYC	1 cut(s)	238
BssSI	CACGAG	1 cut(s)	1404
Bst2BI	CACGAG	1 cut(s)	1404
Bst2UI	CCWGG	2 cut(s)	167, 243
Bst4CI	ACNGT	7 cut(s)	291, 563, 914, 977, 1040, 1193, 1578
Bst6I	CTCTTC	1 cut(s)	90
BstACI	GRCGYC	1 cut(s)	238
BstBAI	YACGTR	1 cut(s)	833
BstC8I	GCNNGC	5 cut(s)	87, 174, 178, 769, 983
BstDEI	CTNAG	6 cut(s)	324, 375, 519, 714, 792, 1347
BstDSI	CCRYGG	2 cut(s)	976, 1039
BstF5I	GGATG	3 cut(s)	691, 1348, 1456
BstH2I	RGCGCY	1 cut(s)	241
BstHHI	GCGC	2 cut(s)	240, 346
BstKTI	GATC	6 cut(s)	129, 149, 415, 462, 1165, 1430
BstMAI	GTCTC	1 cut(s)	1151
BstMBI	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
BstNI	CCWGG	2 cut(s)	167, 243
BstNSI	RCATGY	1 cut(s)	1106
BstPAI	GACNNNNGTC	1 cut(s)	1191
BstSCI	CCNGG	4 cut(s)	38, 165, 241, 346
BstV1I	GCAGC	6 cut(s)	295, 301, 328, 374, 445, 1303
BstV2I	GAAGAC	3 cut(s)	285, 1131, 1219
BstX2I	RGATCY	2 cut(s)	412, 1427
BstYI	RGATCY	2 cut(s)	412, 1427
Bsu36I	CCTNAGG	1 cut(s)	519
BsuI	GTATCC	1 cut(s)	868
BsuRI	GGCC	2 cut(s)	180, 230
BtgI	CCRYGG	2 cut(s)	976, 1039
BtrI	CACGTC	2 cut(s)	896, 1148
BtsCI	GGATG	3 cut(s)	691, 1348, 1456
BveI	ACCTGC	2 cut(s)	1533, 1544
Cac8I	GCNNGC	5 cut(s)	87, 174, 178, 769, 983
CaiI	CAGNNNCTG	1 cut(s)	806
CfoI	GCGC	2 cut(s)	240, 346
Cfr10I	RCCGGY	1 cut(s)	176
CseI	GACGC	1 cut(s)	1265
CviAII	CATG	9 cut(s)	393, 695, 725, 788, 851, 1103, 1166, 1217, 1355
DdeI	CTNAG	6 cut(s)	324, 375, 519, 714, 792, 1347
DinI	GGCGCC	1 cut(s)	239
DpnI	GATC	6 cut(s)	128, 148, 414, 461, 1164, 1429
DpnII	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
EaeI	YGGCCR	1 cut(s)	228
Eam1104I	CTCTTC	1 cut(s)	90
EarI	CTCTTC	1 cut(s)	90
EciI	GGCGGA	2 cut(s)	89, 292
Ecl136II	GAGCTC	2 cut(s)	115, 163
Eco24I	GRGCYC	3 cut(s)	117, 165, 355
Eco53kI	GAGCTC	2 cut(s)	115, 163
Eco57I	CTGAAG	2 cut(s)	828, 1569
Eco81I	CCTNAGG	1 cut(s)	519
EcoICRI	GAGCTC	2 cut(s)	115, 163
EcoRII	CCWGG	2 cut(s)	165, 241
EcoT22I	ATGCAT	2 cut(s)	394, 1570
EcoT38I	GRGCYC	3 cut(s)	117, 165, 355
EgeI	GGCGCC	1 cut(s)	239
EheI	GGCGCC	1 cut(s)	239
FaeI	CATG	9 cut(s)	396, 698, 728, 791, 854, 1106, 1169, 1220, 1358
FalI	AAGNNNNNCTT	4 cut(s)	254, 286, 1430, 1462
FaqI	GGGAC	1 cut(s)	170
FatI	CATG	9 cut(s)	392, 694, 724, 787, 850, 1102, 1165, 1216, 1354
FokI	GGATG	3 cut(s)	698, 1355, 1463
FriOI	GRGCYC	3 cut(s)	117, 165, 355
FspBI	CTAG	2 cut(s)	1320, 1464
GlaI	GCGC	2 cut(s)	239, 345
GsuI	CTGGAG	1 cut(s)	149
HaeII	RGCGCY	1 cut(s)	241
HaeIII	GGCC	2 cut(s)	180, 230
HapII	CCGG	6 cut(s)	39, 49, 177, 222, 347, 583
HgaI	GACGC	1 cut(s)	1265
HhaI	GCGC	2 cut(s)	240, 346
Hin1I	GRCGYC	1 cut(s)	238
Hin1II	CATG	9 cut(s)	396, 698, 728, 791, 854, 1106, 1169, 1220, 1358
Hin6I	GCGC	2 cut(s)	238, 344
HinP1I	GCGC	2 cut(s)	238, 344
HindIII	AAGCTT	1 cut(s)	401
HpaII	CCGG	6 cut(s)	39, 49, 177, 222, 347, 583
HphI	GGTGA	2 cut(s)	265, 1568
Hpy188III	TCNNGA	4 cut(s)	49, 222, 463, 478
Hpy99I	CGWCG	8 cut(s)	113, 134, 900, 1152, 1193, 1281, 1521, 1578
HpyAV	CCTTC	6 cut(s)	19, 64, 280, 535, 1378, 1477
HpyCH4III	ACNGT	7 cut(s)	291, 563, 914, 977, 1040, 1193, 1578
HpyCH4IV	ACGT	7 cut(s)	63, 593, 757, 832, 895, 1147, 1375
HpyCH4V	TGCA	6 cut(s)	392, 1336, 1354, 1403, 1537, 1568
HpyF3I	CTNAG	6 cut(s)	324, 375, 519, 714, 792, 1347
HpySE526I	ACGT	7 cut(s)	63, 593, 757, 832, 895, 1147, 1375
Hsp92I	GRCGYC	1 cut(s)	238
Hsp92II	CATG	9 cut(s)	396, 698, 728, 791, 854, 1106, 1169, 1220, 1358
HspAI	GCGC	2 cut(s)	238, 344
KasI	GGCGCC	1 cut(s)	237
Kpn2I	TCCGGA	2 cut(s)	48, 221
KroI	GCCGGC	1 cut(s)	176
KroNI	GCCGGC	1 cut(s)	178
Kzo9I	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
LmnI	GCTCC	5 cut(s)	42, 76, 120, 168, 169
Lsp1109I	GCAGC	6 cut(s)	295, 301, 328, 374, 445, 1303
LweI	GCATC	7 cut(s)	341, 379, 662, 1323, 1333, 1390, 1441
MaeI	CTAG	2 cut(s)	1320, 1464
MaeII	ACGT	7 cut(s)	63, 593, 757, 832, 895, 1147, 1375
MaeIII	GTNAC	1 cut(s)	533
MalI	GATC	6 cut(s)	128, 148, 414, 461, 1164, 1429
MboI	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
MflI	RGATCY	2 cut(s)	412, 1427
MhlI	GDGCHC	6 cut(s)	117, 165, 355, 769, 895, 1147
MluCI	AATT	4 cut(s)	256, 815, 1222, 1441
Mly113I	GGCGCC	1 cut(s)	238
Mph1103I	ATGCAT	2 cut(s)	394, 1570
MroI	TCCGGA	2 cut(s)	48, 221
MroNI	GCCGGC	1 cut(s)	176
MseI	TTAA	4 cut(s)	429, 666, 918, 1458
MspA1I	CMGCKG	3 cut(s)	308, 314, 617
MspI	CCGG	6 cut(s)	39, 49, 177, 222, 347, 583
MspR9I	CCNGG	4 cut(s)	40, 167, 243, 348
Mva1269I	GAATGC	6 cut(s)	670, 859, 922, 985, 1048, 1111
MvaI	CCWGG	2 cut(s)	167, 243
NaeI	GCCGGC	1 cut(s)	178
NarI	GGCGCC	1 cut(s)	238
NciI	CCSGG	2 cut(s)	40, 348
NdeII	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
NgoMIV	GCCGGC	1 cut(s)	176
NlaIII	CATG	9 cut(s)	396, 698, 728, 791, 854, 1106, 1169, 1220, 1358
NlaIV	GGNNCC	3 cut(s)	44, 239, 414
NmuCI	GTSAC	1 cut(s)	533
NsiI	ATGCAT	2 cut(s)	394, 1570
NspI	RCATGY	1 cut(s)	1106
PaqCI	CACCTGC	1 cut(s)	1544
PctI	GAATGC	6 cut(s)	670, 859, 922, 985, 1048, 1111
PdiI	GCCGGC	1 cut(s)	178
PfoI	TCCNGGA	1 cut(s)	165
PluTI	GGCGCC	1 cut(s)	241
Ppu21I	YACGTR	1 cut(s)	833
PshAI	GACNNNNGTC	1 cut(s)	1191
Psp124BI	GAGCTC	2 cut(s)	117, 165
Psp1406I	AACGTT	1 cut(s)	1375
Psp6I	CCWGG	2 cut(s)	165, 241
PspGI	CCWGG	2 cut(s)	165, 241
PspN4I	GGNNCC	3 cut(s)	44, 239, 414
PstNI	CAGNNNCTG	1 cut(s)	806
PsuI	RGATCY	2 cut(s)	412, 1427
PvuII	CAGCTG	1 cut(s)	617
SacI	GAGCTC	2 cut(s)	117, 165
SaqAI	TTAA	4 cut(s)	429, 666, 918, 1458
Sau3AI	GATC	6 cut(s)	126, 146, 412, 459, 1162, 1427
ScrFI	CCNGG	4 cut(s)	40, 167, 243, 348
SduI	GDGCHC	6 cut(s)	117, 165, 355, 769, 895, 1147
SfaNI	GCATC	7 cut(s)	341, 379, 662, 1323, 1333, 1390, 1441
SfoI	GGCGCC	1 cut(s)	239
SpeI	ACTAGT	1 cut(s)	1463
Sse9I	AATT	4 cut(s)	256, 815, 1222, 1441
SsiI	CCGC	9 cut(s)	74, 90, 118, 246, 303, 306, 312, 321, 1434
SspDI	GGCGCC	1 cut(s)	237
SspMI	CTAG	2 cut(s)	1320, 1464
SstI	GAGCTC	2 cut(s)	117, 165
StyD4I	CCNGG	4 cut(s)	38, 165, 241, 346
TaaI	ACNGT	7 cut(s)	291, 563, 914, 977, 1040, 1193, 1578
TaiI	ACGT	7 cut(s)	66, 596, 760, 835, 898, 1150, 1378
TasI	AATT	4 cut(s)	256, 815, 1222, 1441
TauI	GCSGC	4 cut(s)	92, 249, 308, 314
Tru1I	TTAA	4 cut(s)	429, 666, 918, 1458
Tru9I	TTAA	4 cut(s)	429, 666, 918, 1458
TseFI	GTSAC	1 cut(s)	533
TseI	GCWGC	6 cut(s)	308, 314, 341, 362, 433, 1316
Tsp45I	GTSAC	1 cut(s)	533
TspDTI	ATGAA	7 cut(s)	702, 765, 828, 1080, 1233, 1275, 1371
TspGWI	ACGGA	2 cut(s)	146, 1505
XapI	RAATTY	2 cut(s)	1222, 1441
XceI	RCATGY	1 cut(s)	1106
XspI	CTAG	2 cut(s)	1320, 1464
Zsp2I	ATGCAT	2 cut(s)	394, 1570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.