Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0006065

Physical Location & Seq

Genomic Coordinates Forward (+)

35629561 .. 35632112

Gene Structure 2552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG178800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1584 bp

ATGGCTTTGAAGTTTCTGAACAAGAAGGGGTGGCACACCGGGAGCCTCCGGAACATAGAGAACGTGTGGAAGGCGGAGCAGAAGCACGCCGCCGAAGAAAAGAAGCTCGACGAGCTCCGCAAACAGATCGTCGAAGAAAGAGAACGATCCGAGTTCCGTGAGCTCCAGGAGCAAGCCGGCCTCGTCCCCAAGCAAGAGCGATTGGAGTTCCTCTACGATTCCGGATTGGCCGTAGGGCGCCCCGGCGGCTCTGATAATTTCAAGGAAGCCTTTACCAAAGCCGAAGAACCGTCTTCTTCCGCCGCCGCAGCCGCCGCCGCCACTGCGAAGGAGGCATCATCGTCTGCGCCGGGGGCTCTGTTCGCAGCAGACAAGCCTCAGTCTGCCAATGATGCATGGAGGAAGCTTCACTCGGATCCTTTGCTTATGATTAAGCAGCGAGAGCAGGAAGCCCTCTCTCGGATCAAGAACAATCCTGTCCAGATGGCTATGATTCGCAAACAAGTTGAAGCAAAGAAGCCTAAGGAAAAAGAGCGTGACAAGAAGGAACATCGGAAGAAACACCGTAAAGATTCTTCAAAGCACCGGAAGCAAACGTCTGATTCAGAAGATGACACAGCTGATATGGAAAGGAGGAAGAATAGTCATCGTAAGGCATCAAAATATGTTAAGCATTCAGAATCAGAGGATGAACCACATAAAAAAGCACATCGAAGTGAGAAGAACCATGTTAAGCAGTCAAATGTTGAAGATGAATCACGTTATAGAGCACGCCAAAGTGGGAAAACCCATGCTAAGTATTCAGATTCTGAAGATGAATTCCGTAGCAAAGCACGTAGTAGTGATAAAAACCATGTTAGGCATTCAGATTTGGAGGATACATCACGTAACAGAGCACGTCGAAGTGAGAAGAACCGTGTTAAGCATTCAGATTCAGAAGATGAGCTACATAACAGAAGAAGTCGAAGTGAGAAGAACCGTGGCAAGCATTCAGATTCAGAAGATGAGCTACATAACAGAAGAAGTCGAAGTGAGAGAAACCGTGGTAAGCATTCAGATTCAGAAGATGAACTACATAACAGAACAAGTCGAAGTGAGAAGAAGCATGTCAAGCATTCAGATTCAGAAGACGGGCTACATAACAGAGCACGTCGAAGTGAGACAGATCATGCCATAACAAGGGCAAAAGACGACGGTCAAGGTATTGTCAAGGGAAGACATGAAAATTCAGAACAAGAAAACTATTCCATGAGAGGTCGAAATGAATCCCACAACAAGCGTCGGTTTGTTCCACCTAAACTTTCAGAAGAAGAAAGAGCTGCTAGGTTAAAGGAGATGCAGATGGATGCTGAGTTGCATGAAGAACAAAGATGGAAACGTTTGAAGAAGGCTGACGAGAATGATGCACGTGAAGCTACCCAAGCTATCAGATCTGGCGGTAAGAATTTCTTGGATGCTGTTAAAACTAGTGTTTATGGTTCTGGGAAGGGAGGAAGCTCAACAATAGAGGAGAGTGTCCGTCGCAGAACACATTATTTGCAGGGCAGGTCTGAAGCAAGTGAAAGCAATGCATTTCGACGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CWC25 Cir_N

Protein Analysis

527

Amino Acids

60.98

Weight (kDa)

9.72

Isoelectric Point (pI)

73.49

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cir_N	PF10197	11 - 46	4.8e-14	N-terminal domain of CBF1 interacting co-repressor CIR
CWC25	PF12542	64 - 159	4.6e-15	Pre-mRNA splicing factor

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006065)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G44195 AT2G44200
fragaria_vesca	FvH4_4g13451
malus_domestica	MD04G1014200.v1.1
prunus_persica	Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161200_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G161500_v2.0.a1 Prupe.1G162300_v2.0.a1
pyrus_communis	pycom04g01100
rosa_chinensis	RchiOBHm_Chr4g0414011
rosa_laevigata	RLG00000008185
rosa_multiflora	Rmu_sc0003264.1_g000022 Rmu_sc0006428.1_g000008
rosa_roxburghii	Rroxscaffold_5G00357930
rosa_rugosa	Rorug04G0125000
rosa_samantha	Rh4AG183500 Rh4BG183000 Rh4CG195500 Rh4DG178800
rosa_wichuraiana	Rw4G015600

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	1536
AccB1I	GGYRCC	1 cut(s)	237
AccIII	TCCGGA	2 cut(s)	48, 221
AclI	AACGTT	1 cut(s)	1378
AclWI	GGATC	4 cut(s)	141, 410, 423, 470
AcoI	YGGCCR	1 cut(s)	228
AcsI	RAATTY	3 cut(s)	818, 1225, 1444
AcuI	CTGAAG	2 cut(s)	831, 1572
AcvI	CACGTG	1 cut(s)	1409
AcyI	GRCGYC	1 cut(s)	238
AfiI	CCNNNNNNNGG	2 cut(s)	459, 1179
AflIII	ACRYGT	1 cut(s)	63
AgsI	TTSAA	6 cut(s)	10, 262, 509, 579, 749, 1384
AhlI	ACTAGT	1 cut(s)	1466
AjiI	CACGTC	2 cut(s)	899, 1151
AjnI	CCWGG	1 cut(s)	165
Alw21I	GWGCWC	5 cut(s)	117, 165, 772, 898, 1150
Alw26I	GTCTC	1 cut(s)	1154
AlwI	GGATC	4 cut(s)	141, 410, 423, 470
AlwNI	CAGNNNCTG	1 cut(s)	809
Aor13HI	TCCGGA	2 cut(s)	48, 221
AoxI	GGCC	2 cut(s)	178, 228
ApeKI	GCWGC	4 cut(s)	308, 365, 436, 1319
ApoI	RAATTY	3 cut(s)	818, 1225, 1444
ArsI	GACNNNNNNTTYG	2 cut(s)	114, 146
AspLEI	GCGC	2 cut(s)	240, 349
AsuC2I	CCSGG	3 cut(s)	40, 243, 351
AxyI	CCTNAGG	1 cut(s)	522
BamHI	GGATCC	1 cut(s)	415
BanI	GGYRCC	1 cut(s)	237
BanII	GRGCYC	3 cut(s)	117, 165, 358
BbrPI	CACGTG	1 cut(s)	1409
BbsI	GAAGAC	3 cut(s)	285, 1134, 1222
Bbv12I	GWGCWC	5 cut(s)	117, 165, 772, 898, 1150
BbvI	GCAGC	4 cut(s)	320, 377, 448, 1306
BccI	CCATC	3 cut(s)	478, 1336, 1365
BceAI	ACGGC	1 cut(s)	215
BcgI	CGANNNNNNTGC	4 cut(s)	109, 143, 1385, 1419
BciT130I	CCWGG	1 cut(s)	167
BciVI	GTATCC	1 cut(s)	871
BcnI	CCSGG	3 cut(s)	40, 243, 351
BcoDI	GTCTC	1 cut(s)	1154
BcuI	ACTAGT	1 cut(s)	1466
BfaI	CTAG	2 cut(s)	1323, 1467
BfoI	RGCGCY	1 cut(s)	241
BfuAI	ACCTGC	1 cut(s)	1536
BfuI	GTATCC	1 cut(s)	871
BglI	GCCNNNNNGGC	1 cut(s)	246
BglII	AGATCT	1 cut(s)	1430
Bme1390I	CCNGG	4 cut(s)	40, 167, 243, 351
BmgBI	CACGTC	2 cut(s)	899, 1151
BmiI	GGNNCC	3 cut(s)	44, 239, 417
BmrFI	CCNGG	4 cut(s)	40, 167, 243, 351
BmsI	GCATC	7 cut(s)	344, 382, 665, 1326, 1336, 1393, 1444
BoxI	GACNNNNGTC	1 cut(s)	1194
BpiI	GAAGAC	3 cut(s)	285, 1134, 1222
BpmI	CTGGAG	1 cut(s)	149
BpuMI	CCSGG	3 cut(s)	40, 243, 351
BsaAI	YACGTR	3 cut(s)	836, 887, 1409
BsaHI	GRCGYC	1 cut(s)	238
BsaJI	CCNNGG	4 cut(s)	241, 350, 979, 1042
BsaWI	WCCGGW	3 cut(s)	48, 221, 585
Bsc4I	CCNNNNNNNGG	2 cut(s)	459, 1179
Bse118I	RCCGGY	1 cut(s)	176
Bse21I	CCTNAGG	1 cut(s)	522
Bse3DI	GCAATG	1 cut(s)	1573
BseAI	TCCGGA	2 cut(s)	48, 221
BseBI	CCWGG	1 cut(s)	167
BseDI	CCNNGG	4 cut(s)	241, 350, 979, 1042
BseGI	GGATG	3 cut(s)	694, 1351, 1459
BseLI	CCNNNNNNNGG	2 cut(s)	459, 1179
BseMI	GCAATG	1 cut(s)	1573
BseMII	CTCAG	2 cut(s)	392, 1341
BseRI	GAGGAG	1 cut(s)	1523
BseXI	GCAGC	4 cut(s)	320, 377, 448, 1306
BshFI	GGCC	2 cut(s)	180, 230
BshNI	GGYRCC	1 cut(s)	237
BsiHKAI	GWGCWC	5 cut(s)	117, 165, 772, 898, 1150
BsiSI	CCGG	7 cut(s)	39, 49, 177, 222, 243, 350, 586
BslFI	GGGAC	1 cut(s)	170
BslI	CCNNNNNNNGG	2 cut(s)	459, 1179
BsmAI	GTCTC	1 cut(s)	1154
BsmFI	GGGAC	1 cut(s)	170
BsmI	GAATGC	6 cut(s)	673, 862, 925, 988, 1051, 1114
BsnI	GGCC	2 cut(s)	180, 230
Bsp1286I	GDGCHC	6 cut(s)	117, 165, 358, 772, 898, 1150
Bsp13I	TCCGGA	2 cut(s)	48, 221
Bsp143I	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
BspANI	GGCC	2 cut(s)	180, 230
BspCNI	CTCAG	2 cut(s)	391, 1342
BspEI	TCCGGA	2 cut(s)	48, 221
BspLI	GGNNCC	3 cut(s)	44, 239, 417
BspMI	ACCTGC	1 cut(s)	1536
BspPI	GGATC	4 cut(s)	141, 410, 423, 470
BspT107I	GGYRCC	1 cut(s)	237
BsrDI	GCAATG	1 cut(s)	1573
BsrFI	RCCGGY	1 cut(s)	176
BssAI	RCCGGY	1 cut(s)	176
BssECI	CCNNGG	4 cut(s)	241, 350, 979, 1042
BssMI	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
BssNI	GRCGYC	1 cut(s)	238
Bst2UI	CCWGG	1 cut(s)	167
Bst4CI	ACNGT	7 cut(s)	291, 566, 917, 980, 1043, 1196, 1581
BstACI	GRCGYC	1 cut(s)	238
BstBAI	YACGTR	3 cut(s)	836, 887, 1409
BstC8I	GCNNGC	5 cut(s)	87, 174, 178, 772, 986
BstDEI	CTNAG	4 cut(s)	378, 522, 795, 1350
BstDSI	CCRYGG	2 cut(s)	979, 1042
BstF5I	GGATG	3 cut(s)	694, 1351, 1459
BstH2I	RGCGCY	1 cut(s)	241
BstHHI	GCGC	2 cut(s)	240, 349
BstKTI	GATC	6 cut(s)	129, 149, 418, 465, 1168, 1433
BstMAI	GTCTC	1 cut(s)	1154
BstMBI	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
BstNI	CCWGG	1 cut(s)	167
BstNSI	RCATGY	1 cut(s)	1109
BstPAI	GACNNNNGTC	1 cut(s)	1194
BstSCI	CCNGG	4 cut(s)	38, 165, 241, 349
BstV1I	GCAGC	4 cut(s)	320, 377, 448, 1306
BstV2I	GAAGAC	3 cut(s)	285, 1134, 1222
BstX2I	RGATCY	2 cut(s)	415, 1430
BstYI	RGATCY	2 cut(s)	415, 1430
Bsu36I	CCTNAGG	1 cut(s)	522
BsuI	GTATCC	1 cut(s)	871
BsuRI	GGCC	2 cut(s)	180, 230
BtgI	CCRYGG	2 cut(s)	979, 1042
BtrI	CACGTC	2 cut(s)	899, 1151
BtsCI	GGATG	3 cut(s)	694, 1351, 1459
BtsI	GCAGTG	1 cut(s)	321
BtsIMutI	CAGTG	1 cut(s)	321
BveI	ACCTGC	1 cut(s)	1536
Cac8I	GCNNGC	5 cut(s)	87, 174, 178, 772, 986
CaiI	CAGNNNCTG	1 cut(s)	809
CfoI	GCGC	2 cut(s)	240, 349
Cfr10I	RCCGGY	1 cut(s)	176
CseI	GACGC	1 cut(s)	1268
CviAII	CATG	9 cut(s)	396, 728, 791, 854, 1106, 1169, 1220, 1249, 1358
DdeI	CTNAG	4 cut(s)	378, 522, 795, 1350
DinI	GGCGCC	1 cut(s)	239
DpnI	GATC	6 cut(s)	128, 148, 417, 464, 1167, 1432
DpnII	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
EaeI	YGGCCR	1 cut(s)	228
EciI	GGCGGA	2 cut(s)	89, 289
Ecl136II	GAGCTC	2 cut(s)	115, 163
Eco24I	GRGCYC	3 cut(s)	117, 165, 358
Eco53kI	GAGCTC	2 cut(s)	115, 163
Eco57I	CTGAAG	2 cut(s)	831, 1572
Eco72I	CACGTG	1 cut(s)	1409
Eco81I	CCTNAGG	1 cut(s)	522
EcoICRI	GAGCTC	2 cut(s)	115, 163
EcoRI	GAATTC	1 cut(s)	818
EcoRII	CCWGG	1 cut(s)	165
EcoT22I	ATGCAT	2 cut(s)	397, 1573
EcoT38I	GRGCYC	3 cut(s)	117, 165, 358
EgeI	GGCGCC	1 cut(s)	239
EheI	GGCGCC	1 cut(s)	239
FaeI	CATG	9 cut(s)	399, 731, 794, 857, 1109, 1172, 1223, 1252, 1361
FalI	AAGNNNNNCTT	4 cut(s)	254, 286, 1433, 1465
FaqI	GGGAC	1 cut(s)	170
FatI	CATG	9 cut(s)	395, 727, 790, 853, 1105, 1168, 1219, 1248, 1357
FokI	GGATG	3 cut(s)	701, 1358, 1466
FriOI	GRGCYC	3 cut(s)	117, 165, 358
FspBI	CTAG	2 cut(s)	1323, 1467
GlaI	GCGC	2 cut(s)	239, 348
GsuI	CTGGAG	1 cut(s)	149
HaeII	RGCGCY	1 cut(s)	241
HaeIII	GGCC	2 cut(s)	180, 230
HapII	CCGG	7 cut(s)	39, 49, 177, 222, 243, 350, 586
HgaI	GACGC	1 cut(s)	1268
HhaI	GCGC	2 cut(s)	240, 349
Hin1I	GRCGYC	1 cut(s)	238
Hin1II	CATG	9 cut(s)	399, 731, 794, 857, 1109, 1172, 1223, 1252, 1361
Hin6I	GCGC	2 cut(s)	238, 347
HinP1I	GCGC	2 cut(s)	238, 347
HindIII	AAGCTT	1 cut(s)	404
HpaII	CCGG	7 cut(s)	39, 49, 177, 222, 243, 350, 586
Hpy188III	TCNNGA	4 cut(s)	49, 222, 466, 481
Hpy99I	CGWCG	8 cut(s)	113, 134, 903, 1155, 1196, 1284, 1524, 1581
HpyAV	CCTTC	6 cut(s)	19, 64, 322, 538, 1381, 1480
HpyCH4III	ACNGT	7 cut(s)	291, 566, 917, 980, 1043, 1196, 1581
HpyCH4IV	ACGT	9 cut(s)	63, 596, 760, 835, 886, 898, 1150, 1378, 1408
HpyCH4V	TGCA	6 cut(s)	395, 1339, 1357, 1406, 1540, 1571
HpyF3I	CTNAG	4 cut(s)	378, 522, 795, 1350
HpySE526I	ACGT	9 cut(s)	63, 596, 760, 835, 886, 898, 1150, 1378, 1408
Hsp92I	GRCGYC	1 cut(s)	238
Hsp92II	CATG	9 cut(s)	399, 731, 794, 857, 1109, 1172, 1223, 1252, 1361
HspAI	GCGC	2 cut(s)	238, 347
KasI	GGCGCC	1 cut(s)	237
Kpn2I	TCCGGA	2 cut(s)	48, 221
KroI	GCCGGC	1 cut(s)	176
KroNI	GCCGGC	1 cut(s)	178
Kzo9I	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
LmnI	GCTCC	5 cut(s)	42, 76, 120, 168, 169
Lsp1109I	GCAGC	4 cut(s)	320, 377, 448, 1306
LweI	GCATC	7 cut(s)	344, 382, 665, 1326, 1336, 1393, 1444
MaeI	CTAG	2 cut(s)	1323, 1467
MaeII	ACGT	9 cut(s)	63, 596, 760, 835, 886, 898, 1150, 1378, 1408
MaeIII	GTNAC	2 cut(s)	536, 887
MalI	GATC	6 cut(s)	128, 148, 417, 464, 1167, 1432
MboI	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
MflI	RGATCY	2 cut(s)	415, 1430
MhlI	GDGCHC	6 cut(s)	117, 165, 358, 772, 898, 1150
MluCI	AATT	4 cut(s)	256, 818, 1225, 1444
Mly113I	GGCGCC	1 cut(s)	238
Mph1103I	ATGCAT	2 cut(s)	397, 1573
MroI	TCCGGA	2 cut(s)	48, 221
MroNI	GCCGGC	1 cut(s)	176
MseI	TTAA	6 cut(s)	432, 669, 732, 921, 1328, 1461
MslI	CAYNNNNRTG	1 cut(s)	714
MspA1I	CMGCKG	1 cut(s)	620
MspI	CCGG	7 cut(s)	39, 49, 177, 222, 243, 350, 586
MspR9I	CCNGG	4 cut(s)	40, 167, 243, 351
Mva1269I	GAATGC	6 cut(s)	673, 862, 925, 988, 1051, 1114
MvaI	CCWGG	1 cut(s)	167
NaeI	GCCGGC	1 cut(s)	178
NarI	GGCGCC	1 cut(s)	238
NciI	CCSGG	3 cut(s)	40, 243, 351
NdeII	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
NgoMIV	GCCGGC	1 cut(s)	176
NlaIII	CATG	9 cut(s)	399, 731, 794, 857, 1109, 1172, 1223, 1252, 1361
NlaIV	GGNNCC	3 cut(s)	44, 239, 417
NmuCI	GTSAC	1 cut(s)	536
NsiI	ATGCAT	2 cut(s)	397, 1573
NspI	RCATGY	1 cut(s)	1109
PctI	GAATGC	6 cut(s)	673, 862, 925, 988, 1051, 1114
PdiI	GCCGGC	1 cut(s)	178
PfoI	TCCNGGA	1 cut(s)	165
PluTI	GGCGCC	1 cut(s)	241
PmaCI	CACGTG	1 cut(s)	1409
PmlI	CACGTG	1 cut(s)	1409
Ppu21I	YACGTR	3 cut(s)	836, 887, 1409
PshAI	GACNNNNGTC	1 cut(s)	1194
Psp124BI	GAGCTC	2 cut(s)	117, 165
Psp1406I	AACGTT	1 cut(s)	1378
Psp6I	CCWGG	1 cut(s)	165
PspCI	CACGTG	1 cut(s)	1409
PspGI	CCWGG	1 cut(s)	165
PspN4I	GGNNCC	3 cut(s)	44, 239, 417
PstNI	CAGNNNCTG	1 cut(s)	809
PsuI	RGATCY	2 cut(s)	415, 1430
PvuII	CAGCTG	1 cut(s)	620
RseI	CAYNNNNRTG	1 cut(s)	714
SacI	GAGCTC	2 cut(s)	117, 165
SaqAI	TTAA	6 cut(s)	432, 669, 732, 921, 1328, 1461
Sau3AI	GATC	6 cut(s)	126, 146, 415, 462, 1165, 1430
ScrFI	CCNGG	4 cut(s)	40, 167, 243, 351
SduI	GDGCHC	6 cut(s)	117, 165, 358, 772, 898, 1150
SfaNI	GCATC	7 cut(s)	344, 382, 665, 1326, 1336, 1393, 1444
SfoI	GGCGCC	1 cut(s)	239
SmiMI	CAYNNNNRTG	1 cut(s)	714
SpeI	ACTAGT	1 cut(s)	1466
Sse9I	AATT	4 cut(s)	256, 818, 1225, 1444
SspDI	GGCGCC	1 cut(s)	237
SspMI	CTAG	2 cut(s)	1323, 1467
SstI	GAGCTC	2 cut(s)	117, 165
StyD4I	CCNGG	4 cut(s)	38, 165, 241, 349
TaaI	ACNGT	7 cut(s)	291, 566, 917, 980, 1043, 1196, 1581
TaiI	ACGT	9 cut(s)	66, 599, 763, 838, 889, 901, 1153, 1381, 1411
TasI	AATT	4 cut(s)	256, 818, 1225, 1444
TauI	GCSGC	7 cut(s)	92, 249, 305, 308, 314, 317, 320
Tru1I	TTAA	6 cut(s)	432, 669, 732, 921, 1328, 1461
Tru9I	TTAA	6 cut(s)	432, 669, 732, 921, 1328, 1461
TscAI	CASTG	1 cut(s)	328
TseFI	GTSAC	1 cut(s)	536
TseI	GCWGC	4 cut(s)	308, 365, 436, 1319
Tsp45I	GTSAC	1 cut(s)	536
TspDTI	ATGAA	7 cut(s)	705, 768, 831, 1083, 1236, 1278, 1374
TspGWI	ACGGA	3 cut(s)	146, 812, 1508
TspRI	CASTG	1 cut(s)	328
XapI	RAATTY	3 cut(s)	818, 1225, 1444
XceI	RCATGY	1 cut(s)	1109
XspI	CTAG	2 cut(s)	1323, 1467
Zsp2I	ATGCAT	2 cut(s)	397, 1573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.