AT2G45150

Belongs to the CDS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0006601
Physical Location & Seq
Forward (+)
18613305 .. 18615770
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G45150.3

Sequence Viewer

Length: 1293 bp
ATGACAAATACGAACACACTCTTCCTCAGACATGATGATGATTCAGAGATTTTGATTTTTCAAGAAAACTCTGTTTTTCCTCTTTTTGTTTTTGCCTCTTGGGGCATTTGGAGAGCAATGGCGACTTTTGCTGAACTTGTTTTATCGACTTCTCGCTGTACATGCCCTTGCCGTTCATTCACTAGAAAACCCCTAATTCGTCCCCCTTTATCTGGTCTGCGTCTCCCCGGTGATACCAAACCATTGTTTCGTTCCGGACTTGGTCGGATTTCTGTTAGCCGGCGTTTCCTCACGGCCGTTGCTCGAGCTGAATCAGACCAGCTTGGTGATGATGACCACTCAAAGGGAATTGATAGAATCCATAACTTGCAGAATGTGGAAGATAAGCAGAAGAAAGCAAGCCAGCTTAAGAAAAGAGTGATCTTTGGTATTGGCATTGGTTTACCTGTTGGATGTGTTGTGTTAGCTGGAGGATGGGTTTTCACTGTAGCTTTAGCATCTTCTGTTTTTATCGGTTCCCGCGAATATTTCGAGCTTGTTAGAAGTAGAGGCATAGCTAAAGGAATGACTCCTCCTCCACGATATGTATCTCGAGTTTGCTCGGTTATATGTGCCCTTATGCCCATACTTACACTGTACTTTGGTAACATTGATATATTGGTGACATCTGCAGCATTTGTTGTTGCAATAGCATTGTTAGTACAAAGAGGATCCCCACGTTTTGCTCAGCTGAGTAGTACAATGTTTGGTCTGTTTTACTGTGGTTATCTCCCTTCTTTCTGGGTTAAGCTTCGCTGTGGTTTAGCTGCTCCTGCGCTTAACACTGGTATCGGAAGGACATGGCCAATTCTTCTTGGTGGTCAAGCTCATTGGACAGTTGGACTTGTGGCAACATTGATTTCTTTCAGCGGTGTAATTGCGACAGACACATTTGCTTTTCTCGGTGGAAAGACTTTTGGTAGGACACCTCTTACTAGTATTAGTCCCAAGAAGACATGGGAAGGAACTATTGTAGGACTTGTTGGTTGTATAGCCATTACCATATTACTCTCTAAATATCTCAGTTGGCCACAATCTCTGTTCAGCTCAGTAGCTTTTGGGTTTCTTAACTTCTTTGGGTCAGTCTTTGGTGATCTTACTGAATCAATGATCAAGCGTGATGCTGGCGTCAAAGACTCTGGTTCACTTATCCCAGGACACGGTGGAATATTAGATAGAGTTGATAGTTACATTTTCACCGGCGCATTAGCTTATTCATTCATCAAAACATCCCTAAAACTTTACGGAGTTTGA

Protein Analysis

430

Amino Acids

46.77

Weight (kDa)

9.72

Isoelectric Point (pI)

33.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CTP_transf_1 PF01148 89 - 374 4.6e-64 Cytidylyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 522
AccIII TCCGGA 1 cut(s) 254
AciI CCGC 2 cut(s) 520, 909
AclWI GGATC 2 cut(s) 705, 718
AcoI YGGCCR 3 cut(s) 294, 842, 1067
AcyI GRCGYC 1 cut(s) 1167
AfaI GTAC 4 cut(s) 160, 638, 702, 739
AfiI CCNNNNNNNGG 3 cut(s) 212, 343, 1199
AflII CTTAAG 1 cut(s) 407
AgsI TTSAA 1 cut(s) 62
AhlI ACTAGT 1 cut(s) 974
AjnI CCWGG 1 cut(s) 1192
Alw26I GTCTC 1 cut(s) 227
AlwI GGATC 2 cut(s) 705, 718
Ama87I CYCGRG 2 cut(s) 303, 591
Aor13HI TCCGGA 1 cut(s) 254
AoxI GGCC 3 cut(s) 294, 842, 1067
ApeKI GCWGC 2 cut(s) 671, 806
AspLEI GCGC 2 cut(s) 817, 1244
AsuC2I CCSGG 1 cut(s) 228
AsuHPI GGTGA 5 cut(s) 242, 338, 673, 1142, 1228
AvaI CYCGRG 2 cut(s) 303, 591
BaeGI GKGCMC 1 cut(s) 616
BaeI ACNNNNGTAYC 2 cut(s) 811, 844
BalI TGGCCA 2 cut(s) 844, 1069
BamHI GGATCC 1 cut(s) 710
BbsI GAAGAC 1 cut(s) 998
BbvI GCAGC 2 cut(s) 683, 793
BccI CCATC 1 cut(s) 468
BceAI ACGGC 3 cut(s) 156, 281, 309
BciT130I CCWGG 1 cut(s) 1194
BclI TGATCA 1 cut(s) 1149
BcnI CCSGG 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 227
BcuI ACTAGT 1 cut(s) 974
BfaI CTAG 2 cut(s) 183, 975
BfmI CTRYAG 2 cut(s) 486, 669
BfrI CTTAAG 1 cut(s) 407
BisI GCNGC 2 cut(s) 672, 807
BlpI GCTNAGC 1 cut(s) 726
BlsI GCNGC 2 cut(s) 673, 808
Bme1390I CCNGG 2 cut(s) 228, 1194
BmeT110I CYCGRG 2 cut(s) 303, 591
BmiI GGNNCC 2 cut(s) 517, 712
BmrFI CCNGG 2 cut(s) 228, 1194
BmsI GCATC 2 cut(s) 506, 1150
BpiI GAAGAC 1 cut(s) 998
BpmI CTGGAG 1 cut(s) 489
Bpu1102I GCTNAGC 1 cut(s) 726
BpuMI CCSGG 1 cut(s) 228
BsaBI GATNNNNATC 1 cut(s) 586
BsaHI GRCGYC 1 cut(s) 1167
BsaJI CCNNGG 2 cut(s) 226, 1192
BsaWI WCCGGW 1 cut(s) 254
BsaXI ACNNNNNCTCC 4 cut(s) 462, 492, 559, 589
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 343, 1199
Bse118I RCCGGY 2 cut(s) 279, 1238
Bse1I ACTGG 1 cut(s) 829
Bse3DI GCAATG 1 cut(s) 123
Bse8I GATNNNNATC 1 cut(s) 586
BseAI TCCGGA 1 cut(s) 254
BseBI CCWGG 1 cut(s) 1194
BseDI CCNNGG 2 cut(s) 226, 1192
BseGI GGATG 3 cut(s) 458, 479, 1268
BseJI GATNNNNATC 1 cut(s) 586
BseLI CCNNNNNNNGG 3 cut(s) 212, 343, 1199
BseMI GCAATG 1 cut(s) 123
BseMII CTCAG 5 cut(s) 40, 722, 740, 1075, 1101
BseNI ACTGG 1 cut(s) 829
BseRI GAGGAG 2 cut(s) 561, 564
BseSI GKGCMC 1 cut(s) 616
BseX3I CGGCCG 1 cut(s) 294
BseXI GCAGC 2 cut(s) 683, 793
Bsh1236I CGCG 1 cut(s) 522
Bsh1285I CGRYCG 1 cut(s) 297
BshFI GGCC 3 cut(s) 296, 844, 1069
BsiEI CGRYCG 1 cut(s) 297
BsiHKCI CYCGRG 2 cut(s) 303, 591
BsiSI CCGG 4 cut(s) 228, 255, 280, 1239
BslFI GGGAC 2 cut(s) 186, 969
BslI CCNNNNNNNGG 3 cut(s) 212, 343, 1199
BsmAI GTCTC 1 cut(s) 227
BsmBI CGTCTC 1 cut(s) 227
BsmFI GGGAC 2 cut(s) 186, 969
BsnI GGCC 3 cut(s) 296, 844, 1069
BsoBI CYCGRG 2 cut(s) 303, 591
Bsp1286I GDGCHC 1 cut(s) 616
Bsp13I TCCGGA 1 cut(s) 254
Bsp1407I TGTACA 1 cut(s) 158
Bsp143I GATC 4 cut(s) 420, 710, 1132, 1149
Bsp1720I GCTNAGC 1 cut(s) 726
BspACI CCGC 2 cut(s) 520, 909
BspANI GGCC 3 cut(s) 296, 844, 1069
BspCNI CTCAG 5 cut(s) 39, 723, 739, 1074, 1100
BspEI TCCGGA 1 cut(s) 254
BspFNI CGCG 1 cut(s) 522
BspLI GGNNCC 2 cut(s) 517, 712
BspMAI CTGCAG 1 cut(s) 673
BspPI GGATC 2 cut(s) 705, 718
BspTI CTTAAG 1 cut(s) 407
BsrDI GCAATG 1 cut(s) 123
BsrFI RCCGGY 2 cut(s) 279, 1238
BsrGI TGTACA 1 cut(s) 158
BsrI ACTGG 1 cut(s) 829
BssAI RCCGGY 2 cut(s) 279, 1238
BssECI CCNNGG 2 cut(s) 226, 1192
BssMI GATC 4 cut(s) 420, 710, 1132, 1149
BssNI GRCGYC 1 cut(s) 1167
Bst2UI CCWGG 1 cut(s) 1194
Bst4CI ACNGT 5 cut(s) 487, 636, 761, 877, 1202
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 1167
BstAFI CTTAAG 1 cut(s) 407
BstAUI TGTACA 1 cut(s) 158
BstC8I GCNNGC 4 cut(s) 281, 400, 404, 1165
BstDEI CTNAG 5 cut(s) 26, 726, 731, 1061, 1087
BstF5I GGATG 3 cut(s) 458, 479, 1268
BstFNI CGCG 1 cut(s) 522
BstHHI GCGC 2 cut(s) 817, 1244
BstKTI GATC 4 cut(s) 423, 713, 1135, 1152
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 4 cut(s) 420, 710, 1132, 1149
BstMCI CGRYCG 1 cut(s) 297
BstMWI GCNNNNNNNGC 3 cut(s) 128, 162, 812
BstNI CCWGG 1 cut(s) 1194
BstNSI RCATGY 1 cut(s) 165
BstSCI CCNGG 2 cut(s) 226, 1192
BstSFI CTRYAG 2 cut(s) 486, 669
BstSLI GKGCMC 1 cut(s) 616
BstUI CGCG 1 cut(s) 522
BstV1I GCAGC 2 cut(s) 683, 793
BstV2I GAAGAC 1 cut(s) 998
BstX2I RGATCY 1 cut(s) 710
BstYI RGATCY 1 cut(s) 710
BstZI CGGCCG 1 cut(s) 294
BsuRI GGCC 3 cut(s) 296, 844, 1069
BtsCI GGATG 3 cut(s) 458, 479, 1268
BtsIMutI CAGTG 3 cut(s) 483, 632, 822
Cac8I GCNNGC 4 cut(s) 281, 400, 404, 1165
CfoI GCGC 2 cut(s) 817, 1244
Cfr10I RCCGGY 2 cut(s) 279, 1238
CseI GACGC 2 cut(s) 209, 1156
Csp6I GTAC 4 cut(s) 159, 637, 701, 738
CviAII CATG 4 cut(s) 32, 162, 840, 996
CviQI GTAC 4 cut(s) 159, 637, 701, 738
DdeI CTNAG 5 cut(s) 26, 726, 731, 1061, 1087
DpnI GATC 4 cut(s) 422, 712, 1134, 1151
DpnII GATC 4 cut(s) 420, 710, 1132, 1149
EaeI YGGCCR 3 cut(s) 294, 842, 1067
EagI CGGCCG 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
EclXI CGGCCG 1 cut(s) 294
Eco52I CGGCCG 1 cut(s) 294
Eco88I CYCGRG 2 cut(s) 303, 591
EcoRII CCWGG 1 cut(s) 1192
Esp3I CGTCTC 1 cut(s) 227
FaeI CATG 4 cut(s) 35, 165, 843, 999
FaqI GGGAC 2 cut(s) 186, 969
FatI CATG 4 cut(s) 31, 161, 839, 995
FauI CCCGC 1 cut(s) 527
FbaI TGATCA 1 cut(s) 1149
Fnu4HI GCNGC 2 cut(s) 672, 807
FokI GGATG 3 cut(s) 465, 486, 1255
Fsp4HI GCNGC 2 cut(s) 672, 807
FspBI CTAG 2 cut(s) 183, 975
GlaI GCGC 2 cut(s) 816, 1243
GluI GCNGC 2 cut(s) 672, 807
GsuI CTGGAG 1 cut(s) 489
HaeIII GGCC 3 cut(s) 296, 844, 1069
HapII CCGG 4 cut(s) 228, 255, 280, 1239
HgaI GACGC 2 cut(s) 209, 1156
HhaI GCGC 2 cut(s) 817, 1244
Hin1I GRCGYC 1 cut(s) 1167
Hin1II CATG 4 cut(s) 35, 165, 843, 999
Hin6I GCGC 2 cut(s) 815, 1242
HinP1I GCGC 2 cut(s) 815, 1242
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 6 cut(s) 41, 311, 357, 568, 1142, 1175
HpaII CCGG 4 cut(s) 228, 255, 280, 1239
HphI GGTGA 5 cut(s) 242, 338, 673, 1142, 1228
Hpy166II GTNNAC 2 cut(s) 443, 1184
Hpy188I TCNGA 5 cut(s) 29, 46, 267, 316, 833
Hpy188III TCNNGA 3 cut(s) 62, 255, 591
Hpy8I GTNNAC 2 cut(s) 443, 1184
HpyAV CCTTC 3 cut(s) 783, 828, 995
HpyCH4III ACNGT 5 cut(s) 487, 636, 761, 877, 1202
HpyCH4IV ACGT 1 cut(s) 718
HpyCH4V TGCA 3 cut(s) 370, 671, 686
HpyF10VI GCNNNNNNNGC 3 cut(s) 128, 162, 812
HpyF3I CTNAG 5 cut(s) 26, 726, 731, 1061, 1087
HpySE526I ACGT 1 cut(s) 718
Hsp92I GRCGYC 1 cut(s) 1167
Hsp92II CATG 4 cut(s) 35, 165, 843, 999
HspAI GCGC 2 cut(s) 815, 1242
Kpn2I TCCGGA 1 cut(s) 254
KroI GCCGGC 1 cut(s) 279
KroNI GCCGGC 1 cut(s) 281
Ksp22I TGATCA 1 cut(s) 1149
Kzo9I GATC 4 cut(s) 420, 710, 1132, 1149
LmnI GCTCC 1 cut(s) 814
Lsp1109I GCAGC 2 cut(s) 683, 793
LweI GCATC 2 cut(s) 506, 1150
MaeI CTAG 2 cut(s) 183, 975
MaeII ACGT 1 cut(s) 718
MaeIII GTNAC 3 cut(s) 644, 661, 1226
MalI GATC 4 cut(s) 422, 712, 1134, 1151
MboI GATC 4 cut(s) 420, 710, 1132, 1149
MboII GAAGA 6 cut(s) 13, 392, 403, 492, 842, 1003
MflI RGATCY 1 cut(s) 710
MhlI GDGCHC 1 cut(s) 616
MlsI TGGCCA 2 cut(s) 844, 1069
MluCI AATT 4 cut(s) 195, 348, 846, 915
MluNI TGGCCA 2 cut(s) 844, 1069
MlyI GAGTC 2 cut(s) 562, 1169
MmeI TCCRAC 3 cut(s) 245, 430, 859
Mox20I TGGCCA 2 cut(s) 844, 1069
MroI TCCGGA 1 cut(s) 254
MroNI GCCGGC 1 cut(s) 279
MscI TGGCCA 2 cut(s) 844, 1069
MseI TTAA 4 cut(s) 408, 786, 819, 1107
MslI CAYNNNNRTG 1 cut(s) 36
Msp20I TGGCCA 2 cut(s) 844, 1069
MspA1I CMGCKG 2 cut(s) 730, 909
MspCI CTTAAG 1 cut(s) 407
MspI CCGG 4 cut(s) 228, 255, 280, 1239
MspR9I CCNGG 2 cut(s) 228, 1194
MvaI CCWGG 1 cut(s) 1194
MvnI CGCG 1 cut(s) 522
MwoI GCNNNNNNNGC 3 cut(s) 128, 162, 812
NaeI GCCGGC 1 cut(s) 281
NciI CCSGG 1 cut(s) 228
NdeII GATC 4 cut(s) 420, 710, 1132, 1149
NgoMIV GCCGGC 1 cut(s) 279
NlaIII CATG 4 cut(s) 35, 165, 843, 999
NlaIV GGNNCC 2 cut(s) 517, 712
NmuCI GTSAC 1 cut(s) 661
NspI RCATGY 1 cut(s) 165
PaeR7I CTCGAG 2 cut(s) 303, 591
PcsI WCGNNNNNNNCGW 1 cut(s) 519
PdiI GCCGGC 1 cut(s) 281
PfeI GAWTC 4 cut(s) 41, 311, 357, 1142
PflFI GACNNNGTC 1 cut(s) 261
PkrI GCNGC 2 cut(s) 673, 808
PleI GAGTC 2 cut(s) 562, 1169
PpsI GAGTC 2 cut(s) 562, 1169
Psp6I CCWGG 1 cut(s) 1192
PspGI CCWGG 1 cut(s) 1192
PspN4I GGNNCC 2 cut(s) 517, 712
PspXI VCTCGAGB 1 cut(s) 303
PstI CTGCAG 1 cut(s) 673
PsuI RGATCY 1 cut(s) 710
PsyI GACNNNGTC 1 cut(s) 261
PvuII CAGCTG 1 cut(s) 730
RsaI GTAC 4 cut(s) 160, 638, 702, 739
RsaNI GTAC 4 cut(s) 159, 637, 701, 738
RseI CAYNNNNRTG 1 cut(s) 36
SaqAI TTAA 4 cut(s) 408, 786, 819, 1107
SatI GCNGC 2 cut(s) 672, 807
Sau3AI GATC 4 cut(s) 420, 710, 1132, 1149
SchI GAGTC 2 cut(s) 562, 1169
ScrFI CCNGG 2 cut(s) 228, 1194
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 2 cut(s) 506, 1150
SfcI CTRYAG 2 cut(s) 486, 669
Sfr274I CTCGAG 2 cut(s) 303, 591
SgrAI CRCCGGYG 1 cut(s) 1238
SlaI CTCGAG 2 cut(s) 303, 591
SmiMI CAYNNNNRTG 1 cut(s) 36
SmlI CTYRAG 3 cut(s) 303, 407, 591
SmoI CTYRAG 3 cut(s) 303, 407, 591
SpeI ACTAGT 1 cut(s) 974
Sse9I AATT 4 cut(s) 195, 348, 846, 915
SsiI CCGC 2 cut(s) 520, 909
SspI AATATT 2 cut(s) 527, 1209
SspMI CTAG 2 cut(s) 183, 975
StyD4I CCNGG 2 cut(s) 226, 1192
TaaI ACNGT 5 cut(s) 487, 636, 761, 877, 1202
TaiI ACGT 1 cut(s) 721
TaqI TCGA 4 cut(s) 146, 304, 531, 592
TasI AATT 4 cut(s) 195, 348, 846, 915
TatI WGTACW 4 cut(s) 158, 636, 700, 737
TfiI GAWTC 4 cut(s) 41, 311, 357, 1142
Tru1I TTAA 4 cut(s) 408, 786, 819, 1107
Tru9I TTAA 4 cut(s) 408, 786, 819, 1107
TscAI CASTG 3 cut(s) 490, 639, 829
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 2 cut(s) 671, 806
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 3 cut(s) 165, 1245, 1249
TspRI CASTG 3 cut(s) 490, 639, 829
Tth111I GACNNNGTC 1 cut(s) 261
Vha464I CTTAAG 1 cut(s) 407
XceI RCATGY 1 cut(s) 165
XhoI CTCGAG 2 cut(s) 303, 591
XspI CTAG 2 cut(s) 183, 975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.