AT3G02590

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0010864
Physical Location & Seq
Forward (+)
549175 .. 550682
1508 bp
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UTR
Exon/CDS
Intron
AT3G02590.1

Sequence Viewer

Length: 840 bp
ATGGCGGCGACTATGGCAGATTATAATGATCAGATCGTCAATGAGACCTCTTTTTACAACCGAATGGTTCTGAGTCACCTTTTGCCGGTGAATCTATGGGAACCTTTACCACATTTCCTCCAGACATGGCTCCGGAACTACCTCGCCGGAAACATACTCTACTTCATCTCCGGCTTCCTCTGGTGCTTCTACATCTATTACCTTAAACTCAACGTTTACGTCCCCAAAGAATCCATTCCTACGAGAAAGGCAATGCTTTTGCAAATATACGTGGCAATGAAGGCTATGCCTTGGTACACTCTTCTTCCAGCTGTCTCTGAGTATATGATCGAGCATGGTTGGACCAAATGTTACTCTACACTTGACCATTTCAACTGGTTCCTCTGTTTCCTCTACATAGCTCTCTATCTTGTTTTAGTTGAGTTTATGATTTATTGGGTTCACAAAGAGCTTCATGACATTAAATTTCTCTATAAGCATCTCCATGCTACCCATCATATGTACAACAAGCAAAACACACTCTCTCCATTTGCCGGGCTCGCATTCCATCCGCTGGACGGGATACTTCAGGCTATACCGCACGTGATAGCGCTGTTTATAGTGCCGATTCATCTCATAACACATCTGAGTCTTTTGTTTTTGGAAGGGATATGGACAGCAAGCATCCATGATTGCATACATGGTAACATCTGGCCTATAATGGGTGCAGGATACCATACCATACACCATACAACATACAAGCATAACTATGGTCATTATACCATATGGATGGACTGGATGTTTGGCTCTCTTATGGTTCCTTTAGCAGAAAAAGACAGTTTCAAGGAGAAAGAAAAGTGA

Protein Analysis

279

Amino Acids

33.11

Weight (kDa)

6.86

Isoelectric Point (pI)

39.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 134 - 263 2.9e-19 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02580 AT3G02590 AT3G02590
fragaria_vesca FvH4_2g26170
malus_domestica MD09G1254800.v1.1 MD17G1247400.v1.1
prunus_persica Prupe.3G144900_v2.0.a1
pyrus_communis pycom09g17070 pycom17g24880
rosa_chinensis RchiOBHm_Chr6g0294591
rosa_laevigata RLG00000011856
rosa_multiflora Rmu_sc0006264.1_g000012 Rmu_sc0007845.1_g000002
rosa_roxburghii Rroxscaffold_7G00173180
rosa_rugosa Rorug06G0246200
rosa_samantha Rh6AG358500 Rh6BG365700 Rh6CG372300 Rh6DG359100
rosa_wichuraiana Rw6G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 24
AccB7I CCANNNNNTGG 1 cut(s) 553
AccIII TCCGGA 1 cut(s) 132
AciI CCGC 3 cut(s) 5, 551, 578
AclI AACGTT 1 cut(s) 213
AcsI RAATTY 1 cut(s) 464
AcuI CTGAAG 1 cut(s) 551
AcvI CACGTG 1 cut(s) 583
AfaI GTAC 2 cut(s) 296, 503
AfeI AGCGCT 1 cut(s) 591
AfiI CCNNNNNNNGG 5 cut(s) 85, 533, 553, 557, 701
AgsI TTSAA 2 cut(s) 373, 823
AluBI AGCT 3 cut(s) 311, 401, 451
AluI AGCT 3 cut(s) 311, 401, 451
Alw26I GTCTC 2 cut(s) 38, 319
Aor13HI TCCGGA 1 cut(s) 132
Aor51HI AGCGCT 1 cut(s) 591
AoxI GGCC 1 cut(s) 692
ApoI RAATTY 1 cut(s) 464
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
Asp700I GAANNNNTTC 1 cut(s) 234
AspLEI GCGC 1 cut(s) 592
AspS9I GGNCC 1 cut(s) 342
AsuC2I CCSGG 1 cut(s) 535
AsuHPI GGTGA 2 cut(s) 68, 100
AvaII GGWCC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 540
BbrPI CACGTG 1 cut(s) 583
BccI CCATC 3 cut(s) 501, 555, 763
BciVI GTATCC 2 cut(s) 555, 704
BclI TGATCA 1 cut(s) 28
BcnI CCSGG 1 cut(s) 535
BcoDI GTCTC 2 cut(s) 38, 319
BfoI RGCGCY 1 cut(s) 593
BfuI GTATCC 2 cut(s) 555, 704
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
Bme1390I CCNGG 1 cut(s) 535
Bme18I GGWCC 1 cut(s) 342
BmgT120I GGNCC 1 cut(s) 342
BmiI GGNNCC 4 cut(s) 102, 131, 380, 798
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 2 cut(s) 487, 672
BpmI CTGGAG 1 cut(s) 104
BpuMI CCSGG 1 cut(s) 535
BsaAI YACGTR 2 cut(s) 271, 583
BsaI GGTCTC 1 cut(s) 38
BsaJI CCNNGG 1 cut(s) 290
BsaWI WCCGGW 1 cut(s) 132
BsaXI ACNNNNNCTCC 6 cut(s) 102, 132, 152, 182, 508, 538
Bsc4I CCNNNNNNNGG 5 cut(s) 85, 533, 553, 557, 701
Bse118I RCCGGY 1 cut(s) 85
Bse1I ACTGG 2 cut(s) 380, 779
Bse3DI GCAATG 2 cut(s) 258, 282
BseAI TCCGGA 1 cut(s) 132
BseDI CCNNGG 1 cut(s) 290
BseGI GGATG 4 cut(s) 547, 663, 774, 783
BseLI CCNNNNNNNGG 5 cut(s) 85, 533, 553, 557, 701
BseMI GCAATG 2 cut(s) 258, 282
BseMII CTCAG 3 cut(s) 62, 309, 617
BseNI ACTGG 2 cut(s) 380, 779
BsgI GTGCAG 1 cut(s) 726
BshFI GGCC 1 cut(s) 694
BsiSI CCGG 5 cut(s) 86, 133, 147, 171, 534
BslFI GGGAC 1 cut(s) 206
BslI CCNNNNNNNGG 5 cut(s) 85, 533, 553, 557, 701
BsmAI GTCTC 2 cut(s) 38, 319
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 542
BsnI GGCC 1 cut(s) 694
Bso31I GGTCTC 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 540
Bsp13I TCCGGA 1 cut(s) 132
Bsp1407I TGTACA 1 cut(s) 501
Bsp143I GATC 3 cut(s) 28, 33, 327
BspACI CCGC 3 cut(s) 5, 551, 578
BspANI GGCC 1 cut(s) 694
BspCNI CTCAG 3 cut(s) 63, 310, 618
BspEI TCCGGA 1 cut(s) 132
BspHI TCATGA 1 cut(s) 454
BspLI GGNNCC 4 cut(s) 102, 131, 380, 798
BspTNI GGTCTC 1 cut(s) 38
BsrDI GCAATG 2 cut(s) 258, 282
BsrFI RCCGGY 1 cut(s) 85
BsrGI TGTACA 1 cut(s) 501
BsrI ACTGG 2 cut(s) 380, 779
BssAI RCCGGY 1 cut(s) 85
BssECI CCNNGG 1 cut(s) 290
BssMI GATC 3 cut(s) 28, 33, 327
BssT1I CCWWGG 1 cut(s) 290
Bst4CI ACNGT 1 cut(s) 818
Bst6I CTCTTC 1 cut(s) 306
BstAUI TGTACA 1 cut(s) 501
BstBAI YACGTR 2 cut(s) 271, 583
BstC8I GCNNGC 2 cut(s) 540, 661
BstDEI CTNAG 3 cut(s) 71, 318, 626
BstF5I GGATG 4 cut(s) 547, 663, 774, 783
BstH2I RGCGCY 1 cut(s) 593
BstHHI GCGC 1 cut(s) 592
BstKTI GATC 3 cut(s) 31, 36, 330
BstMAI GTCTC 2 cut(s) 38, 319
BstMBI GATC 3 cut(s) 28, 33, 327
BstMWI GCNNNNNNNGC 3 cut(s) 14, 281, 539
BstSCI CCNGG 1 cut(s) 533
BstXI CCANNNNNNTGG 1 cut(s) 769
BsuI GTATCC 2 cut(s) 555, 704
BsuRI GGCC 1 cut(s) 694
BtsCI GGATG 4 cut(s) 547, 663, 774, 783
Cac8I GCNNGC 2 cut(s) 540, 661
CciI TCATGA 1 cut(s) 454
CfoI GCGC 1 cut(s) 592
Cfr10I RCCGGY 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 342
Csp6I GTAC 2 cut(s) 295, 502
CviAII CATG 6 cut(s) 126, 335, 455, 485, 668, 680
CviQI GTAC 2 cut(s) 295, 502
DdeI CTNAG 3 cut(s) 71, 318, 626
DpnI GATC 3 cut(s) 30, 35, 329
DpnII GATC 3 cut(s) 28, 33, 327
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco130I CCWWGG 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 540
Eco31I GGTCTC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 342
Eco47III AGCGCT 1 cut(s) 591
Eco57I CTGAAG 1 cut(s) 551
Eco72I CACGTG 1 cut(s) 583
EcoT14I CCWWGG 1 cut(s) 290
EcoT38I GRGCYC 1 cut(s) 540
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 6 cut(s) 129, 338, 458, 488, 671, 683
FaqI GGGAC 1 cut(s) 206
FatI CATG 6 cut(s) 125, 334, 454, 484, 667, 679
FauNDI CATATG 2 cut(s) 498, 764
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 4 cut(s) 534, 650, 781, 790
FriOI GRGCYC 1 cut(s) 540
Fsp4HI GCNGC 1 cut(s) 6
GlaI GCGC 1 cut(s) 591
GluI GCNGC 1 cut(s) 6
GsuI CTGGAG 1 cut(s) 104
HaeII RGCGCY 1 cut(s) 593
HaeIII GGCC 1 cut(s) 694
HapII CCGG 5 cut(s) 86, 133, 147, 171, 534
HhaI GCGC 1 cut(s) 592
Hin1II CATG 6 cut(s) 129, 338, 458, 488, 671, 683
Hin6I GCGC 1 cut(s) 590
HinP1I GCGC 1 cut(s) 590
HinfI GANTC 5 cut(s) 73, 91, 230, 607, 628
HpaII CCGG 5 cut(s) 86, 133, 147, 171, 534
HphI GGTGA 2 cut(s) 68, 100
Hpy166II GTNNAC 3 cut(s) 217, 297, 442
Hpy188I TCNGA 4 cut(s) 33, 72, 319, 627
Hpy188III TCNNGA 3 cut(s) 121, 133, 455
Hpy8I GTNNAC 3 cut(s) 217, 297, 442
HpyAV CCTTC 2 cut(s) 274, 638
HpyCH4III ACNGT 1 cut(s) 818
HpyCH4IV ACGT 4 cut(s) 213, 219, 270, 582
HpyCH4V TGCA 3 cut(s) 262, 675, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 281, 539
HpyF3I CTNAG 3 cut(s) 71, 318, 626
HpySE526I ACGT 4 cut(s) 213, 219, 270, 582
Hsp92II CATG 6 cut(s) 129, 338, 458, 488, 671, 683
HspAI GCGC 1 cut(s) 590
Kpn2I TCCGGA 1 cut(s) 132
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 3 cut(s) 28, 33, 327
LmnI GCTCC 1 cut(s) 135
LweI GCATC 2 cut(s) 487, 672
MaeII ACGT 4 cut(s) 213, 219, 270, 582
MaeIII GTNAC 3 cut(s) 74, 350, 683
MalI GATC 3 cut(s) 30, 35, 329
MboI GATC 3 cut(s) 28, 33, 327
MboII GAAGA 2 cut(s) 293, 296
MhlI GDGCHC 1 cut(s) 540
MluCI AATT 1 cut(s) 464
MlyI GAGTC 2 cut(s) 82, 637
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 6 cut(s) 58, 128, 152, 188, 392, 401
MroI TCCGGA 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 234
MseI TTAA 2 cut(s) 204, 462
MslI CAYNNNNRTG 3 cut(s) 483, 747, 767
MspA1I CMGCKG 2 cut(s) 311, 553
MspI CCGG 5 cut(s) 86, 133, 147, 171, 534
MspR9I CCNGG 1 cut(s) 535
Mva1269I GAATGC 1 cut(s) 542
MwoI GCNNNNNNNGC 3 cut(s) 14, 281, 539
NciI CCSGG 1 cut(s) 535
NdeI CATATG 2 cut(s) 498, 764
NdeII GATC 3 cut(s) 28, 33, 327
NlaIII CATG 6 cut(s) 129, 338, 458, 488, 671, 683
NlaIV GGNNCC 4 cut(s) 102, 131, 380, 798
NmuCI GTSAC 1 cut(s) 74
PagI TCATGA 1 cut(s) 454
PctI GAATGC 1 cut(s) 542
PdmI GAANNNNTTC 1 cut(s) 234
PfeI GAWTC 3 cut(s) 91, 230, 607
PflMI CCANNNNNTGG 1 cut(s) 553
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 2 cut(s) 81, 636
PmaCI CACGTG 1 cut(s) 583
PmlI CACGTG 1 cut(s) 583
PpsI GAGTC 2 cut(s) 81, 636
Ppu21I YACGTR 2 cut(s) 271, 583
PsiI TTATAA 1 cut(s) 24
Psp1406I AACGTT 1 cut(s) 213
PspCI CACGTG 1 cut(s) 583
PspN4I GGNNCC 4 cut(s) 102, 131, 380, 798
PspPI GGNCC 1 cut(s) 342
PvuII CAGCTG 1 cut(s) 311
RsaI GTAC 2 cut(s) 296, 503
RsaNI GTAC 2 cut(s) 295, 502
RseI CAYNNNNRTG 3 cut(s) 483, 747, 767
SaqAI TTAA 2 cut(s) 204, 462
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 3 cut(s) 28, 33, 327
Sau96I GGNCC 1 cut(s) 342
SchI GAGTC 2 cut(s) 82, 637
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 1 cut(s) 540
SfaNI GCATC 2 cut(s) 487, 672
SinI GGWCC 1 cut(s) 342
SmiMI CAYNNNNRTG 3 cut(s) 483, 747, 767
Sse9I AATT 1 cut(s) 464
SsiI CCGC 3 cut(s) 5, 551, 578
StyD4I CCNGG 1 cut(s) 533
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 818
TaiI ACGT 4 cut(s) 216, 222, 273, 585
TaqI TCGA 1 cut(s) 330
TasI AATT 1 cut(s) 464
TatI WGTACW 1 cut(s) 501
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 91, 230, 607
Tru1I TTAA 2 cut(s) 204, 462
Tru9I TTAA 2 cut(s) 204, 462
TseFI GTSAC 1 cut(s) 74
Tsp45I GTSAC 1 cut(s) 74
TspDTI ATGAA 4 cut(s) 154, 293, 443, 599
Van91I CCANNNNNTGG 1 cut(s) 553
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 1 cut(s) 464
XmnI GAANNNNTTC 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.