RLG00000011856

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010864
Physical Location & Seq
Reverse (-)
13337050 .. 13339069
2020 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011856

Sequence Viewer

Length: 843 bp
ATGGAGTCCTCGTCGTCGCCGTCGCTGTCGTCCTCGTACCTGAACTTGTTCTTGGAGGAGACAAGCTTCTACAACCGCATTGTCCTCGACAGCCTGTTGCCCAACTGGGTGTGGACCCCGCTGCCCCATTTCTTCCAGTCGTGGCTGCGCAACTACCTCGGCGGCACCTTAATCTACCTCGTCTCCGGCTTCCTCTGGTGCTTCTACATTTACTACCTCAAACGCAACGTCTACCTCCCGAAAGATGCCATTCCCTCTAATAAAGCCATGCTTTTGCAAATATATGTTGCCATGAAGGCCATGCCTTGGTACAGTGCTCTTCCGTCTCTCTCTGAGTACATGGTTGAAAATGGCTGGGCGAGGTGCTTCTCAAGAATAAGTGATGTAGGTTGGGTTGCTTACCTCATCTATTTATCAATTTATCTTATAATCGTGGAGTTTGGAATCTATTGGATGCATAGGGAGTTGCATGACATAAAGCCTCTGTATAAATGGCTTCATTATATACATCACATTTACAACAAGCAGAACACCCTCTCTCCATTTGCTGGTTTGGCTTTCCACCCACTTGATGGGATACTGCAGGCAGTTCCGCATGTTATAGCTCTGTTTCTTGTGCCAATGCATTTTACTACTCACATAGCGCTCCTATTTATTGAAGCCATATGGACAGCAAATATTCACGATTGCATCCATGCCAAAATATGGCCTGTGATGGGTGCTGGCTACCACACCATTCATCATACTACCTATCGCCATAATTATGGCCATTATACTATATGGATGGACTGGATGTTTGGAACTCTTCGTGACCCTATCAATGAGGAATCAAAGGTGTTATGA

Protein Analysis

281

Amino Acids

33.04

Weight (kDa)

6.99

Isoelectric Point (pI)

57.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 140 - 268 1.4e-20 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02580 AT3G02590 AT3G02590
fragaria_vesca FvH4_2g26170
malus_domestica MD09G1254800.v1.1 MD17G1247400.v1.1
prunus_persica Prupe.3G144900_v2.0.a1
pyrus_communis pycom09g17070 pycom17g24880
rosa_chinensis RchiOBHm_Chr6g0294591
rosa_laevigata RLG00000011856
rosa_multiflora Rmu_sc0006264.1_g000012 Rmu_sc0007845.1_g000002
rosa_roxburghii Rroxscaffold_7G00173180
rosa_rugosa Rorug06G0246200
rosa_samantha Rh6AG358500 Rh6BG365700 Rh6CG372300 Rh6DG359100
rosa_wichuraiana Rw6G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
Acc16I TGCGCA 1 cut(s) 149
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 4 cut(s) 306, 548, 572, 705
AccI GTMKAC 1 cut(s) 231
AciI CCGC 4 cut(s) 76, 119, 162, 593
AcoI YGGCCR 1 cut(s) 766
AfaI GTAC 3 cut(s) 38, 311, 338
AfeI AGCGCT 1 cut(s) 645
AfiI CCNNNNNNNGG 5 cut(s) 306, 548, 572, 705, 716
AgsI TTSAA 2 cut(s) 347, 659
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 2 cut(s) 66, 605
AluI AGCT 2 cut(s) 66, 605
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 3 cut(s) 53, 187, 330
Aor51HI AGCGCT 1 cut(s) 645
AoxI GGCC 3 cut(s) 297, 707, 766
ApeKI GCWGC 2 cut(s) 121, 145
ArsI GACNNNNNNTTYG 4 cut(s) 213, 245, 779, 811
Asp700I GAANNNNTTC 1 cut(s) 47
AspLEI GCGC 2 cut(s) 150, 646
AspS9I GGNCC 1 cut(s) 114
AvaII GGWCC 1 cut(s) 114
BalI TGGCCA 1 cut(s) 768
BanI GGYRCC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 108, 132
BccI CCATC 3 cut(s) 566, 709, 778
BceAI ACGGC 1 cut(s) 4
BcgI CGANNNNNNTGC 4 cut(s) 67, 101, 139, 173
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 3 cut(s) 53, 187, 330
BfmI CTRYAG 1 cut(s) 581
BfoI RGCGCY 1 cut(s) 647
BfuI GTATCC 1 cut(s) 570
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 3 cut(s) 122, 146, 163
BlsI GCNGC 3 cut(s) 123, 147, 164
Bme18I GGWCC 1 cut(s) 114
BmgT120I GGNCC 1 cut(s) 114
BmiI GGNNCC 2 cut(s) 116, 166
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 3 cut(s) 235, 444, 699
BmuI ACTGGG 1 cut(s) 115
BpuEI CTTGAG 1 cut(s) 355
BsaJI CCNNGG 2 cut(s) 157, 305
BsaXI ACNNNNNCTCC 5 cut(s) 26, 167, 197, 523, 553
Bsc4I CCNNNNNNNGG 5 cut(s) 306, 548, 572, 705, 716
Bse1I ACTGG 3 cut(s) 110, 136, 794
BseDI CCNNGG 2 cut(s) 157, 305
BseGI GGATG 4 cut(s) 459, 690, 789, 798
BseLI CCNNNNNNNGG 5 cut(s) 306, 548, 572, 705, 716
BseMII CTCAG 1 cut(s) 324
BseNI ACTGG 3 cut(s) 110, 136, 794
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 2 cut(s) 108, 132
BseYI CCCAGC 1 cut(s) 354
BshFI GGCC 3 cut(s) 299, 709, 768
BshNI GGYRCC 1 cut(s) 164
BsiHKAI GWGCWC 1 cut(s) 319
BsiSI CCGG 1 cut(s) 186
BslI CCNNNNNNNGG 5 cut(s) 306, 548, 572, 705, 716
BsmAI GTCTC 3 cut(s) 53, 187, 330
BsmBI CGTCTC 2 cut(s) 187, 330
BsnI GGCC 3 cut(s) 299, 709, 768
Bsp1286I GDGCHC 1 cut(s) 319
BspACI CCGC 4 cut(s) 76, 119, 162, 593
BspANI GGCC 3 cut(s) 299, 709, 768
BspCNI CTCAG 1 cut(s) 325
BspLI GGNNCC 2 cut(s) 116, 166
BspMAI CTGCAG 1 cut(s) 585
BspQI GCTCTTC 1 cut(s) 324
BspT107I GGYRCC 1 cut(s) 164
BsrI ACTGG 3 cut(s) 110, 136, 794
BssECI CCNNGG 2 cut(s) 157, 305
BssT1I CCWWGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 2 cut(s) 324, 810
BstC8I GCNNGC 2 cut(s) 585, 724
BstDEI CTNAG 1 cut(s) 333
BstF5I GGATG 4 cut(s) 459, 690, 789, 798
BstH2I RGCGCY 1 cut(s) 647
BstHHI GCGC 2 cut(s) 150, 646
BstMAI GTCTC 3 cut(s) 53, 187, 330
BstMWI GCNNNNNNNGC 2 cut(s) 296, 554
BstNSI RCATGY 1 cut(s) 599
BstSFI CTRYAG 1 cut(s) 581
BstV1I GCAGC 2 cut(s) 108, 132
BstXI CCANNNNNNTGG 1 cut(s) 764
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 3 cut(s) 299, 709, 768
BtsCI GGATG 4 cut(s) 459, 690, 789, 798
BtsIMutI CAGTG 1 cut(s) 319
Cac8I GCNNGC 2 cut(s) 585, 724
CfoI GCGC 2 cut(s) 150, 646
Cfr13I GGNCC 1 cut(s) 114
Csp6I GTAC 3 cut(s) 37, 310, 337
CviAII CATG 7 cut(s) 268, 292, 301, 340, 470, 596, 695
CviQI GTAC 3 cut(s) 37, 310, 337
DdeI CTNAG 1 cut(s) 333
EaeI YGGCCR 1 cut(s) 766
Eam1104I CTCTTC 2 cut(s) 324, 810
EarI CTCTTC 2 cut(s) 324, 810
Eco130I CCWWGG 1 cut(s) 305
Eco47I GGWCC 1 cut(s) 114
Eco47III AGCGCT 1 cut(s) 645
EcoT14I CCWWGG 1 cut(s) 305
EcoT22I ATGCAT 2 cut(s) 459, 627
ErhI CCWWGG 1 cut(s) 305
Esp3I CGTCTC 2 cut(s) 187, 330
FaeI CATG 7 cut(s) 271, 295, 304, 343, 473, 599, 698
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FatI CATG 7 cut(s) 267, 291, 300, 339, 469, 595, 694
FauI CCCGC 1 cut(s) 126
FauNDI CATATG 1 cut(s) 665
FblI GTMKAC 1 cut(s) 231
Fnu4HI GCNGC 3 cut(s) 122, 146, 163
FokI GGATG 4 cut(s) 466, 677, 796, 805
Fsp4HI GCNGC 3 cut(s) 122, 146, 163
FspI TGCGCA 1 cut(s) 149
GlaI GCGC 2 cut(s) 149, 645
GluI GCNGC 3 cut(s) 122, 146, 163
GsaI CCCAGC 1 cut(s) 358
HaeII RGCGCY 1 cut(s) 647
HaeIII GGCC 3 cut(s) 299, 709, 768
HapII CCGG 1 cut(s) 186
HhaI GCGC 2 cut(s) 150, 646
Hin1II CATG 7 cut(s) 271, 295, 304, 343, 473, 599, 698
Hin6I GCGC 2 cut(s) 148, 644
HinP1I GCGC 2 cut(s) 148, 644
HindIII AAGCTT 1 cut(s) 64
HinfI GANTC 3 cut(s) 5, 444, 827
HpaII CCGG 1 cut(s) 186
Hpy166II GTNNAC 2 cut(s) 114, 232
Hpy188I TCNGA 1 cut(s) 334
Hpy188III TCNNGA 4 cut(s) 238, 372, 683, 809
Hpy8I GTNNAC 2 cut(s) 114, 232
Hpy99I CGWCG 3 cut(s) 16, 19, 25
HpyAV CCTTC 1 cut(s) 289
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 1 cut(s) 228
HpyCH4V TGCA 6 cut(s) 277, 457, 469, 583, 625, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 554
HpyF3I CTNAG 1 cut(s) 333
HpySE526I ACGT 1 cut(s) 228
Hsp92II CATG 7 cut(s) 271, 295, 304, 343, 473, 599, 698
HspAI GCGC 2 cut(s) 148, 644
LguI GCTCTTC 1 cut(s) 324
LmnI GCTCC 1 cut(s) 651
Lsp1109I GCAGC 2 cut(s) 108, 132
LweI GCATC 3 cut(s) 235, 444, 699
MaeII ACGT 1 cut(s) 228
MaeIII GTNAC 1 cut(s) 809
MboII GAAGA 3 cut(s) 124, 311, 797
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 1 cut(s) 768
MluCI AATT 2 cut(s) 417, 760
MluNI TGGCCA 1 cut(s) 768
MlyI GAGTC 1 cut(s) 14
Mox20I TGGCCA 1 cut(s) 768
Mph1103I ATGCAT 2 cut(s) 459, 627
MroXI GAANNNNTTC 1 cut(s) 47
MscI TGGCCA 1 cut(s) 768
MseI TTAA 1 cut(s) 170
MslI CAYNNNNRTG 1 cut(s) 762
Msp20I TGGCCA 1 cut(s) 768
MspA1I CMGCKG 1 cut(s) 121
MspI CCGG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 296, 554
NdeI CATATG 1 cut(s) 665
NlaIII CATG 7 cut(s) 271, 295, 304, 343, 473, 599, 698
NlaIV GGNNCC 2 cut(s) 116, 166
NmeAIII GCCGAG 1 cut(s) 138
NmuCI GTSAC 1 cut(s) 809
NsbI TGCGCA 1 cut(s) 149
NsiI ATGCAT 2 cut(s) 459, 627
NspI RCATGY 1 cut(s) 599
PciSI GCTCTTC 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PdmI GAANNNNTTC 1 cut(s) 47
PfeI GAWTC 2 cut(s) 444, 827
PflMI CCANNNNNTGG 4 cut(s) 306, 548, 572, 705
PkrI GCNGC 3 cut(s) 123, 147, 164
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PsiI TTATAA 1 cut(s) 428
PspFI CCCAGC 1 cut(s) 354
PspN4I GGNNCC 2 cut(s) 116, 166
PspPI GGNCC 1 cut(s) 114
PstI CTGCAG 1 cut(s) 585
RsaI GTAC 3 cut(s) 38, 311, 338
RsaNI GTAC 3 cut(s) 37, 310, 337
RseI CAYNNNNRTG 1 cut(s) 762
SapI GCTCTTC 1 cut(s) 324
SaqAI TTAA 1 cut(s) 170
SatI GCNGC 3 cut(s) 122, 146, 163
Sau96I GGNCC 1 cut(s) 114
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 3 cut(s) 235, 444, 699
SfcI CTRYAG 1 cut(s) 581
SinI GGWCC 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 762
SmlI CTYRAG 1 cut(s) 370
SmoI CTYRAG 1 cut(s) 370
Sse9I AATT 2 cut(s) 417, 760
SsiI CCGC 4 cut(s) 76, 119, 162, 593
SspI AATATT 1 cut(s) 679
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 1 cut(s) 231
TaqI TCGA 1 cut(s) 87
TasI AATT 2 cut(s) 417, 760
TatI WGTACW 1 cut(s) 336
TauI GCSGC 1 cut(s) 165
TfiI GAWTC 2 cut(s) 444, 827
Tru1I TTAA 1 cut(s) 170
Tru9I TTAA 1 cut(s) 170
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 1 cut(s) 809
TseI GCWGC 2 cut(s) 121, 145
Tsp45I GTSAC 1 cut(s) 809
TspDTI ATGAA 3 cut(s) 308, 488, 728
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 1 cut(s) 319
Van91I CCANNNNNTGG 4 cut(s) 306, 548, 572, 705
VpaK11BI GGWCC 1 cut(s) 114
XceI RCATGY 1 cut(s) 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XmiI GTMKAC 1 cut(s) 231
XmnI GAANNNNTTC 1 cut(s) 47
Zsp2I ATGCAT 2 cut(s) 459, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.