Rw6G031280

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0010864
Physical Location & Seq
Forward (+)
55265583 .. 55267530
1948 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G031280.1

Sequence Viewer

Length: 819 bp
ATGGAGTCCTCGTACCTGAACTTGTTCGTGGAGGAGACAAGCTTCTACAACCGCATTGTCCTCGACAGCCTGTTGCCCAACTGGGTGTGGGTCCCGCTGCCCCACTTCTTCCAGTCTTGGCTGCGCAACTACCTCGGCGGCACCTTAATCTACCTCGTCTCCGGCTTCCTCTGGTGCTTCTACATTTACTACCTCAAACGCAACGTCTACCTCCCGAAAGATGCCATTCCCTCTAAGAAAGCCATGCTTTTGCAAATATATGTTGCCATGAAGGCCATGCCTTGGTACTGTGCTCTTCCGTCTCTCTCAGAGTACATGGTTGAAAATGGCTGGGCAAGGTGCTTCTCAAGAATAAGTGATGTAGGTTGGGTTGCTTACCTCATCTACTTATCAATTTATCTTATAATCGTGGAGTTTGGAATCTATTGGATGCATAGGGAGTTGCATGACATAAAGCCTCTGTATAAATGGCTTCATAATATACATCACATTTACAACAAGCAGAACACCCTCTCTCCATTTGCTGGTTTGGCTTTCCACCCACTTGATGGGATACTGCAGGCAGTTCCGCATGTTATAGCTCTGTTTCTTGTGCCAATGCATTTTACTACTCACATAGCGCTCCTATTTATTGAAGCCATATGGACAGCAAATATTCACGATTGCATCCATGCCAAAATATGGCCTGTGATGGGTGCTGGCTACCACACCATTCATCATACTACCTATCGCCATAATTATGGCCATTATACTATATGGATGGACTGGATGTTTGGAACTCTTCGTGACCCTATCAATGAGGAATCAAAGGTCTTATGA

Protein Analysis

272

Amino Acids

32.27

Weight (kDa)

7.3

Isoelectric Point (pI)

55.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 132 - 260 6.8e-21 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02580 AT3G02590 AT3G02590
fragaria_vesca FvH4_2g26170
malus_domestica MD09G1254800.v1.1 MD17G1247400.v1.1
prunus_persica Prupe.3G144900_v2.0.a1
pyrus_communis pycom09g17070 pycom17g24880
rosa_chinensis RchiOBHm_Chr6g0294591
rosa_laevigata RLG00000011856
rosa_multiflora Rmu_sc0006264.1_g000012 Rmu_sc0007845.1_g000002
rosa_roxburghii Rroxscaffold_7G00173180
rosa_rugosa Rorug06G0246200
rosa_samantha Rh6AG358500 Rh6BG365700 Rh6CG372300 Rh6DG359100
rosa_wichuraiana Rw6G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
Acc16I TGCGCA 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 140
AccB7I CCANNNNNTGG 4 cut(s) 282, 524, 548, 681
AccI GTMKAC 1 cut(s) 207
AciI CCGC 4 cut(s) 52, 95, 138, 569
AcoI YGGCCR 1 cut(s) 742
AfaI GTAC 3 cut(s) 14, 287, 314
AfeI AGCGCT 1 cut(s) 621
AfiI CCNNNNNNNGG 5 cut(s) 282, 524, 548, 681, 692
AgsI TTSAA 2 cut(s) 323, 635
AluBI AGCT 2 cut(s) 42, 581
AluI AGCT 2 cut(s) 42, 581
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 3 cut(s) 29, 163, 306
Aor51HI AGCGCT 1 cut(s) 621
AoxI GGCC 3 cut(s) 273, 683, 742
ApeKI GCWGC 2 cut(s) 97, 121
ArsI GACNNNNNNTTYG 4 cut(s) 189, 221, 755, 787
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 2 cut(s) 126, 622
AspS9I GGNCC 1 cut(s) 91
AvaII GGWCC 1 cut(s) 91
BalI TGGCCA 1 cut(s) 744
BanI GGYRCC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 84, 108
BccI CCATC 3 cut(s) 542, 685, 754
BcgI CGANNNNNNTGC 4 cut(s) 43, 77, 115, 149
BciVI GTATCC 1 cut(s) 546
BcoDI GTCTC 3 cut(s) 29, 163, 306
BfmI CTRYAG 1 cut(s) 557
BfoI RGCGCY 1 cut(s) 623
BfuI GTATCC 1 cut(s) 546
BglI GCCNNNNNGGC 1 cut(s) 272
BisI GCNGC 3 cut(s) 98, 122, 139
BlsI GCNGC 3 cut(s) 99, 123, 140
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 3 cut(s) 92, 93, 142
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 3 cut(s) 211, 420, 675
BmuI ACTGGG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 133, 281
BsaXI ACNNNNNCTCC 5 cut(s) 26, 143, 173, 499, 529
Bsc4I CCNNNNNNNGG 5 cut(s) 282, 524, 548, 681, 692
Bse1I ACTGG 3 cut(s) 86, 112, 770
BseDI CCNNGG 2 cut(s) 133, 281
BseGI GGATG 4 cut(s) 435, 666, 765, 774
BseLI CCNNNNNNNGG 5 cut(s) 282, 524, 548, 681, 692
BseMII CTCAG 1 cut(s) 321
BseNI ACTGG 3 cut(s) 86, 112, 770
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 84, 108
BseYI CCCAGC 1 cut(s) 330
BshFI GGCC 3 cut(s) 275, 685, 744
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 5 cut(s) 282, 524, 548, 681, 692
BsmAI GTCTC 3 cut(s) 29, 163, 306
BsmBI CGTCTC 2 cut(s) 163, 306
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 3 cut(s) 275, 685, 744
Bsp1286I GDGCHC 1 cut(s) 295
BspACI CCGC 4 cut(s) 52, 95, 138, 569
BspANI GGCC 3 cut(s) 275, 685, 744
BspCNI CTCAG 1 cut(s) 320
BspLI GGNNCC 3 cut(s) 92, 93, 142
BspMAI CTGCAG 1 cut(s) 561
BspQI GCTCTTC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 140
BsrI ACTGG 3 cut(s) 86, 112, 770
BssECI CCNNGG 2 cut(s) 133, 281
BssT1I CCWWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 2 cut(s) 300, 786
BstC8I GCNNGC 2 cut(s) 561, 700
BstDEI CTNAG 2 cut(s) 234, 307
BstF5I GGATG 4 cut(s) 435, 666, 765, 774
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 2 cut(s) 126, 622
BstMAI GTCTC 3 cut(s) 29, 163, 306
BstMWI GCNNNNNNNGC 2 cut(s) 272, 530
BstNSI RCATGY 1 cut(s) 575
BstSFI CTRYAG 1 cut(s) 557
BstV1I GCAGC 2 cut(s) 84, 108
BstXI CCANNNNNNTGG 1 cut(s) 740
BsuI GTATCC 1 cut(s) 546
BsuRI GGCC 3 cut(s) 275, 685, 744
BtsCI GGATG 4 cut(s) 435, 666, 765, 774
Cac8I GCNNGC 2 cut(s) 561, 700
CfoI GCGC 2 cut(s) 126, 622
Cfr13I GGNCC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 13, 286, 313
CviAII CATG 7 cut(s) 244, 268, 277, 316, 446, 572, 671
CviQI GTAC 3 cut(s) 13, 286, 313
DdeI CTNAG 2 cut(s) 234, 307
EaeI YGGCCR 1 cut(s) 742
Eam1104I CTCTTC 2 cut(s) 300, 786
EarI CTCTTC 2 cut(s) 300, 786
Eco130I CCWWGG 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 91
Eco47III AGCGCT 1 cut(s) 621
EcoO109I RGGNCCY 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 281
EcoT22I ATGCAT 2 cut(s) 435, 603
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 2 cut(s) 163, 306
FaeI CATG 7 cut(s) 247, 271, 280, 319, 449, 575, 674
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 1 cut(s) 77
FatI CATG 7 cut(s) 243, 267, 276, 315, 445, 571, 670
FauI CCCGC 1 cut(s) 102
FauNDI CATATG 1 cut(s) 641
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 3 cut(s) 98, 122, 139
FokI GGATG 4 cut(s) 442, 653, 772, 781
Fsp4HI GCNGC 3 cut(s) 98, 122, 139
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 2 cut(s) 125, 621
GluI GCNGC 3 cut(s) 98, 122, 139
GsaI CCCAGC 1 cut(s) 334
HaeII RGCGCY 1 cut(s) 623
HaeIII GGCC 3 cut(s) 275, 685, 744
HapII CCGG 1 cut(s) 162
HhaI GCGC 2 cut(s) 126, 622
Hin1II CATG 7 cut(s) 247, 271, 280, 319, 449, 575, 674
Hin6I GCGC 2 cut(s) 124, 620
HinP1I GCGC 2 cut(s) 124, 620
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 3 cut(s) 5, 420, 803
HpaII CCGG 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 4 cut(s) 214, 348, 659, 785
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 6 cut(s) 253, 433, 445, 559, 601, 666
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 530
HpyF3I CTNAG 2 cut(s) 234, 307
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 7 cut(s) 247, 271, 280, 319, 449, 575, 674
HspAI GCGC 2 cut(s) 124, 620
KflI GGGWCCC 1 cut(s) 91
LguI GCTCTTC 1 cut(s) 300
LmnI GCTCC 1 cut(s) 627
Lsp1109I GCAGC 2 cut(s) 84, 108
LweI GCATC 3 cut(s) 211, 420, 675
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 785
MboII GAAGA 3 cut(s) 100, 287, 773
MhlI GDGCHC 1 cut(s) 295
MlsI TGGCCA 1 cut(s) 744
MluCI AATT 2 cut(s) 393, 736
MluNI TGGCCA 1 cut(s) 744
MlyI GAGTC 1 cut(s) 14
Mox20I TGGCCA 1 cut(s) 744
Mph1103I ATGCAT 2 cut(s) 435, 603
MroXI GAANNNNTTC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 744
MseI TTAA 1 cut(s) 146
MslI CAYNNNNRTG 1 cut(s) 738
Msp20I TGGCCA 1 cut(s) 744
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 1 cut(s) 162
MwoI GCNNNNNNNGC 2 cut(s) 272, 530
NdeI CATATG 1 cut(s) 641
NlaIII CATG 7 cut(s) 247, 271, 280, 319, 449, 575, 674
NlaIV GGNNCC 3 cut(s) 92, 93, 142
NmeAIII GCCGAG 1 cut(s) 114
NmuCI GTSAC 1 cut(s) 785
NsbI TGCGCA 1 cut(s) 125
NsiI ATGCAT 2 cut(s) 435, 603
NspI RCATGY 1 cut(s) 575
PciSI GCTCTTC 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 2 cut(s) 420, 803
PflMI CCANNNNNTGG 4 cut(s) 282, 524, 548, 681
PkrI GCNGC 3 cut(s) 99, 123, 140
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 91
PsiI TTATAA 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 91
PspFI CCCAGC 1 cut(s) 330
PspN4I GGNNCC 3 cut(s) 92, 93, 142
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
PstI CTGCAG 1 cut(s) 561
RsaI GTAC 3 cut(s) 14, 287, 314
RsaNI GTAC 3 cut(s) 13, 286, 313
RseI CAYNNNNRTG 1 cut(s) 738
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 1 cut(s) 146
SatI GCNGC 3 cut(s) 98, 122, 139
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 3 cut(s) 211, 420, 675
SfcI CTRYAG 1 cut(s) 557
SinI GGWCC 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 738
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 2 cut(s) 393, 736
SsiI CCGC 4 cut(s) 52, 95, 138, 569
SspI AATATT 1 cut(s) 655
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 63
TasI AATT 2 cut(s) 393, 736
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 2 cut(s) 420, 803
Tru1I TTAA 1 cut(s) 146
Tru9I TTAA 1 cut(s) 146
TseFI GTSAC 1 cut(s) 785
TseI GCWGC 2 cut(s) 97, 121
Tsp45I GTSAC 1 cut(s) 785
TspDTI ATGAA 3 cut(s) 284, 464, 704
TspGWI ACGGA 1 cut(s) 288
Van91I CCANNNNNTGG 4 cut(s) 282, 524, 548, 681
VpaK11BI GGWCC 1 cut(s) 91
XceI RCATGY 1 cut(s) 575
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 23
Zsp2I ATGCAT 2 cut(s) 435, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.