Rh6AG358500

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0010864
Physical Location & Seq
Forward (+)
55745515 .. 55748360
2846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG358500.1

Sequence Viewer

Length: 645 bp
ATGGAGTCCTCGTACCTGAACTTGTTCGTGGAGGAGACAAGCTTCTACAACCGCATTGTCCTCGACAGCCTGTTGCCCAACTGGGTGTGGGTCCCGCTGCCCCACTTCTTCCAGTCGTGGCTGCGCAACTACCTCGGCGGCACCTTAATCTACCTCGTCTCCGGCTTCCTCTGGTGCTTCTACATTTACTACCTCAAACGCAACGTCTACCTCCCGAAAGATGCCATTCCCTCTAATAAAGCCATGCTTTTGCAAATATATGTTGCTATGAAGGCCATGCCTTGGTACTGTGCTCTTCCGTCTCTCTCGGAGTACATGGTTGAAAATGGCTGGGCAAGGTGCTTCTCAAGAATAAGTGATGTAGGTTGGGTTGCTTACCTCATCTATTTATCAATTTATCTTATAATCGTGGAGTTTGGAATCTATTGGATGCATAGGGAGTTGCATGACATAAAGCCTCTGTATAAATGGCTTCATAATATACATCACATTTACAACAAGCAGAACACCCTCTCTCCATTTGCTGGTAAGTTGTCAATTACTACTTTACTTGATTTCATGCCTTTTGTCAATTTAACATCTATCTCATCTAATTTGGATGCATATATATATGCACATATATCTTGGTCTTGTTTGATTTATTAG

Protein Analysis

214

Amino Acids

25.44

Weight (kDa)

7.68

Isoelectric Point (pI)

56.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 132 - 207 1.4e-08 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02580 AT3G02590 AT3G02590
fragaria_vesca FvH4_2g26170
malus_domestica MD09G1254800.v1.1 MD17G1247400.v1.1
prunus_persica Prupe.3G144900_v2.0.a1
pyrus_communis pycom09g17070 pycom17g24880
rosa_chinensis RchiOBHm_Chr6g0294591
rosa_laevigata RLG00000011856
rosa_multiflora Rmu_sc0006264.1_g000012 Rmu_sc0007845.1_g000002
rosa_roxburghii Rroxscaffold_7G00173180
rosa_rugosa Rorug06G0246200
rosa_samantha Rh6AG358500 Rh6BG365700 Rh6CG372300 Rh6DG359100
rosa_wichuraiana Rw6G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 404
Acc16I TGCGCA 1 cut(s) 125
AccB1I GGYRCC 1 cut(s) 140
AccB7I CCANNNNNTGG 2 cut(s) 282, 524
AccI GTMKAC 1 cut(s) 207
AciI CCGC 3 cut(s) 52, 95, 138
AfaI GTAC 3 cut(s) 14, 287, 314
AfiI CCNNNNNNNGG 2 cut(s) 282, 524
AgsI TTSAA 1 cut(s) 323
AluBI AGCT 1 cut(s) 42
AluI AGCT 1 cut(s) 42
Alw21I GWGCWC 1 cut(s) 295
Alw26I GTCTC 3 cut(s) 29, 163, 306
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 2 cut(s) 97, 121
ArsI GACNNNNNNTTYG 2 cut(s) 189, 221
Asp700I GAANNNNTTC 1 cut(s) 23
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 91
AvaII GGWCC 1 cut(s) 91
BanI GGYRCC 1 cut(s) 140
Bbv12I GWGCWC 1 cut(s) 295
BbvI GCAGC 2 cut(s) 84, 108
BcgI CGANNNNNNTGC 4 cut(s) 43, 77, 115, 149
BcoDI GTCTC 3 cut(s) 29, 163, 306
BisI GCNGC 3 cut(s) 98, 122, 139
BlsI GCNGC 3 cut(s) 99, 123, 140
Bme18I GGWCC 1 cut(s) 91
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 3 cut(s) 92, 93, 142
BmrI ACTGGG 1 cut(s) 91
BmsI GCATC 3 cut(s) 211, 420, 589
BmuI ACTGGG 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 133, 281
BsaXI ACNNNNNCTCC 5 cut(s) 26, 143, 173, 499, 529
Bsc4I CCNNNNNNNGG 2 cut(s) 282, 524
Bse1I ACTGG 2 cut(s) 86, 112
BseDI CCNNGG 2 cut(s) 133, 281
BseGI GGATG 2 cut(s) 435, 604
BseLI CCNNNNNNNGG 2 cut(s) 282, 524
BseNI ACTGG 2 cut(s) 86, 112
BseRI GAGGAG 1 cut(s) 47
BseXI GCAGC 2 cut(s) 84, 108
BseYI CCCAGC 1 cut(s) 330
BshFI GGCC 1 cut(s) 275
BshNI GGYRCC 1 cut(s) 140
BsiHKAI GWGCWC 1 cut(s) 295
BsiSI CCGG 1 cut(s) 162
BslFI GGGAC 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 282, 524
BsmAI GTCTC 3 cut(s) 29, 163, 306
BsmBI CGTCTC 2 cut(s) 163, 306
BsmFI GGGAC 1 cut(s) 77
BsnI GGCC 1 cut(s) 275
Bsp1286I GDGCHC 1 cut(s) 295
BspACI CCGC 3 cut(s) 52, 95, 138
BspANI GGCC 1 cut(s) 275
BspLI GGNNCC 3 cut(s) 92, 93, 142
BspQI GCTCTTC 1 cut(s) 300
BspT107I GGYRCC 1 cut(s) 140
BsrI ACTGG 2 cut(s) 86, 112
BssECI CCNNGG 2 cut(s) 133, 281
BssT1I CCWWGG 1 cut(s) 281
Bst4CI ACNGT 1 cut(s) 290
Bst6I CTCTTC 1 cut(s) 300
BstF5I GGATG 2 cut(s) 435, 604
BstHHI GCGC 1 cut(s) 126
BstMAI GTCTC 3 cut(s) 29, 163, 306
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstV1I GCAGC 2 cut(s) 84, 108
BsuRI GGCC 1 cut(s) 275
BtsCI GGATG 2 cut(s) 435, 604
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 91
Csp6I GTAC 3 cut(s) 13, 286, 313
CviAII CATG 5 cut(s) 244, 277, 316, 446, 559
CviJI RGCY 9 cut(s) 42, 69, 121, 165, 242, 275, 330, 457, 472
CviKI_1 RGCY 9 cut(s) 42, 69, 121, 165, 242, 275, 330, 457, 472
CviQI GTAC 3 cut(s) 13, 286, 313
Eam1104I CTCTTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 300
Eco130I CCWWGG 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 91
EcoO109I RGGNCCY 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 281
EcoT22I ATGCAT 2 cut(s) 435, 604
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 2 cut(s) 163, 306
FaeI CATG 5 cut(s) 247, 280, 319, 449, 562
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 1 cut(s) 77
FatI CATG 5 cut(s) 243, 276, 315, 445, 558
FauI CCCGC 1 cut(s) 102
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 3 cut(s) 98, 122, 139
FokI GGATG 2 cut(s) 442, 611
Fsp4HI GCNGC 3 cut(s) 98, 122, 139
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 1 cut(s) 125
GluI GCNGC 3 cut(s) 98, 122, 139
GsaI CCCAGC 1 cut(s) 334
HaeIII GGCC 1 cut(s) 275
HapII CCGG 1 cut(s) 162
HhaI GCGC 1 cut(s) 126
Hin1II CATG 5 cut(s) 247, 280, 319, 449, 562
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 40
HinfI GANTC 2 cut(s) 5, 420
HpaII CCGG 1 cut(s) 162
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 2 cut(s) 214, 348
Hpy8I GTNNAC 1 cut(s) 208
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 1 cut(s) 290
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 5 cut(s) 253, 433, 445, 602, 614
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 5 cut(s) 247, 280, 319, 449, 562
HspAI GCGC 1 cut(s) 124
KflI GGGWCCC 1 cut(s) 91
LguI GCTCTTC 1 cut(s) 300
LpnPI CCDG 8 cut(s) 29, 67, 83, 125, 157, 175, 316, 510
Lsp1109I GCAGC 2 cut(s) 84, 108
LweI GCATC 3 cut(s) 211, 420, 589
MaeII ACGT 1 cut(s) 204
MboII GAAGA 2 cut(s) 100, 287
MhlI GDGCHC 1 cut(s) 295
MluCI AATT 4 cut(s) 393, 537, 571, 592
MlyI GAGTC 1 cut(s) 14
Mph1103I ATGCAT 2 cut(s) 435, 604
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 2 cut(s) 146, 575
MspA1I CMGCKG 1 cut(s) 97
MspI CCGG 1 cut(s) 162
MwoI GCNNNNNNNGC 1 cut(s) 272
NlaIII CATG 5 cut(s) 247, 280, 319, 449, 562
NlaIV GGNNCC 3 cut(s) 92, 93, 142
NmeAIII GCCGAG 1 cut(s) 114
NsbI TGCGCA 1 cut(s) 125
NsiI ATGCAT 2 cut(s) 435, 604
PciSI GCTCTTC 1 cut(s) 300
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 420
PflMI CCANNNNNTGG 2 cut(s) 282, 524
PkrI GCNGC 3 cut(s) 99, 123, 140
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PpuMI RGGWCCY 1 cut(s) 91
PsiI TTATAA 1 cut(s) 404
Psp5II RGGWCCY 1 cut(s) 91
PspFI CCCAGC 1 cut(s) 330
PspN4I GGNNCC 3 cut(s) 92, 93, 142
PspPI GGNCC 1 cut(s) 91
PspPPI RGGWCCY 1 cut(s) 91
RsaI GTAC 3 cut(s) 14, 287, 314
RsaNI GTAC 3 cut(s) 13, 286, 313
SapI GCTCTTC 1 cut(s) 300
SaqAI TTAA 2 cut(s) 146, 575
SatI GCNGC 3 cut(s) 98, 122, 139
Sau96I GGNCC 1 cut(s) 91
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 1 cut(s) 295
SfaNI GCATC 3 cut(s) 211, 420, 589
SinI GGWCC 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 346
SmoI CTYRAG 1 cut(s) 346
Sse9I AATT 4 cut(s) 393, 537, 571, 592
SsiI CCGC 3 cut(s) 52, 95, 138
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 290
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 63
TasI AATT 4 cut(s) 393, 537, 571, 592
TatI WGTACW 1 cut(s) 312
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 1 cut(s) 420
Tru1I TTAA 2 cut(s) 146, 575
Tru9I TTAA 2 cut(s) 146, 575
TseI GCWGC 2 cut(s) 97, 121
TspDTI ATGAA 3 cut(s) 284, 464, 547
TspGWI ACGGA 1 cut(s) 288
Van91I CCANNNNNTGG 2 cut(s) 282, 524
VpaK11BI GGWCC 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 207
XmnI GAANNNNTTC 1 cut(s) 23
Zsp2I ATGCAT 2 cut(s) 435, 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.