Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010864

Physical Location & Seq

Genomic Coordinates Forward (+)

32633850 .. 32642863

Gene Structure 9014 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1254800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGGAGGACTCCTACCTGAATCCGTTCTTCGAGGAGACGACGTTTTACAACCGCATTGTGCTCGGCACCATCGTCCCCAATAGAGTGTGGGACCCACTCCCCCACTTCTTCCAGACGTGGCTGCGCAACTACGTCGGCGGGACCTTGATCTACCTCGTCTCCGGCTTCCTTTGGTGCCTGTACATTTACTACCTGAAACGCAACGTTTATCTCCCCAAAGATGCCATTCCCTCTAATAAGGCCATGCTTTTGCAAATATATGTTGCCATGAAGGCCATGCCTTGGTACAGTGCTCTTCCAACTGTTTCTGAGTACATGGTTGAAAATGGCTGGGCTAAGTGCTTTGCAAGAATAAGTGATGTTGGTTGGCCCGCTTACCTCTTGTATTTAGCAATTTATCTTGTATTTGTGGAGTTCGGAATTTATTGGATGCACAGGGAGTTGCATGACATAAAACCTCTGTATAAATATCTTCATGCTACCCATCACATCTACAACAAGCAGAACACCCTCTCTCCGTTTGCTGGTTTGGCTTTTCACCCGATTGATGGGATACTGCAGGCATTTCCACATGTTTTAGCTCTATTTCTAGTACCAACGCATTTTACAACCCACATAGCGCTCCTATTTATCGAGGCCATATGGACAGCGAACATTCACGATTGCATCCATGCCAAAATATGGCCTGTCATGGGTGCCGGCTACCACACCATACATCATACTACATACCGCCATAACTATGGCCATTTTACCATATGGATGGATTGGATGTGTGGAACGCTTCGTGACCCCTTGGACGATGAATCAAAGGTCTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000248 GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0006629 GO:0006694 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0012505 GO:0016020 GO:0016125 GO:0016126 GO:0016491 GO:0016705 GO:0016717 GO:0031984 GO:0042175 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0070704 GO:0071704 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 ko01130 map00100 map01100 map01110 map01130

Pfam Domains

Protein Families

FA_hydroxylase

Protein Analysis

273

Amino Acids

32.03

Weight (kDa)

6.77

Isoelectric Point (pI)

44.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FA_hydroxylase	PF04116	131 - 260	1.3e-20	Fatty acid hydroxylase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010864)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G02580 AT3G02590 AT3G02590
fragaria_vesca	FvH4_2g26170
malus_domestica	MD09G1254800.v1.1 MD17G1247400.v1.1
prunus_persica	Prupe.3G144900_v2.0.a1
pyrus_communis	pycom09g17070 pycom17g24880
rosa_chinensis	RchiOBHm_Chr6g0294591
rosa_laevigata	RLG00000011856
rosa_multiflora	Rmu_sc0006264.1_g000012 Rmu_sc0007845.1_g000002
rosa_roxburghii	Rroxscaffold_7G00173180
rosa_rugosa	Rorug06G0246200
rosa_samantha	Rh6AG358500 Rh6BG365700 Rh6CG372300 Rh6DG359100
rosa_wichuraiana	Rw6G031280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	125
AccB1I	GGYRCC	3 cut(s)	65, 174, 695
AccB7I	CCANNNNNTGG	2 cut(s)	282, 681
AciI	CCGC	4 cut(s)	52, 138, 372, 730
AclI	AACGTT	1 cut(s)	204
AcoI	YGGCCR	1 cut(s)	742
AcsI	RAATTY	1 cut(s)	420
AfaI	GTAC	4 cut(s)	182, 287, 314, 594
AfeI	AGCGCT	1 cut(s)	621
AfiI	CCNNNNNNNGG	5 cut(s)	282, 524, 548, 681, 692
AflIII	ACRYGT	1 cut(s)	571
AgsI	TTSAA	1 cut(s)	323
AjiI	CACGTC	1 cut(s)	117
AluBI	AGCT	1 cut(s)	581
AluI	AGCT	1 cut(s)	581
Alw21I	GWGCWC	2 cut(s)	63, 295
Alw26I	GTCTC	2 cut(s)	29, 163
Aor51HI	AGCGCT	1 cut(s)	621
AoxI	GGCC	6 cut(s)	240, 273, 368, 636, 683, 742
ApeKI	GCWGC	1 cut(s)	121
ApoI	RAATTY	1 cut(s)	420
Asp700I	GAANNNNTTC	1 cut(s)	23
AspLEI	GCGC	2 cut(s)	126, 622
AspS9I	GGNCC	3 cut(s)	91, 141, 369
AsuHPI	GGTGA	1 cut(s)	530
AvaII	GGWCC	2 cut(s)	91, 141
BalI	TGGCCA	1 cut(s)	744
BanI	GGYRCC	3 cut(s)	65, 174, 695
Bbv12I	GWGCWC	2 cut(s)	63, 295
BbvI	GCAGC	1 cut(s)	108
BccI	CCATC	4 cut(s)	77, 492, 542, 754
BcgI	CGANNNNNNTGC	4 cut(s)	43, 77, 115, 149
BciVI	GTATCC	1 cut(s)	546
BcoDI	GTCTC	2 cut(s)	29, 163
BfaI	CTAG	1 cut(s)	590
BfmI	CTRYAG	1 cut(s)	557
BfoI	RGCGCY	1 cut(s)	623
BfuI	GTATCC	1 cut(s)	546
BglI	GCCNNNNNGGC	1 cut(s)	272
BisI	GCNGC	1 cut(s)	122
BlsI	GCNGC	1 cut(s)	123
Bme18I	GGWCC	2 cut(s)	91, 141
BmgBI	CACGTC	1 cut(s)	117
BmgT120I	GGNCC	3 cut(s)	91, 141, 369
BmiI	GGNNCC	6 cut(s)	67, 92, 93, 142, 176, 697
BmsI	GCATC	3 cut(s)	211, 420, 675
BsaJI	CCNNGG	2 cut(s)	281, 792
BsaXI	ACNNNNNCTCC	6 cut(s)	26, 56, 143, 173, 499, 529
Bsc4I	CCNNNNNNNGG	5 cut(s)	282, 524, 548, 681, 692
Bse118I	RCCGGY	1 cut(s)	698
BseDI	CCNNGG	2 cut(s)	281, 792
BseGI	GGATG	4 cut(s)	435, 666, 765, 774
BseLI	CCNNNNNNNGG	5 cut(s)	282, 524, 548, 681, 692
BseMII	CTCAG	1 cut(s)	300
BseRI	GAGGAG	1 cut(s)	47
BseXI	GCAGC	1 cut(s)	108
BseYI	CCCAGC	1 cut(s)	330
BshFI	GGCC	6 cut(s)	242, 275, 370, 638, 685, 744
BshNI	GGYRCC	3 cut(s)	65, 174, 695
BsiHKAI	GWGCWC	2 cut(s)	63, 295
BsiSI	CCGG	2 cut(s)	162, 699
BslFI	GGGAC	3 cut(s)	59, 104, 154
BslI	CCNNNNNNNGG	5 cut(s)	282, 524, 548, 681, 692
BsmAI	GTCTC	2 cut(s)	29, 163
BsmBI	CGTCTC	2 cut(s)	29, 163
BsmFI	GGGAC	3 cut(s)	59, 104, 154
BsnI	GGCC	6 cut(s)	242, 275, 370, 638, 685, 744
Bsp1286I	GDGCHC	2 cut(s)	63, 295
Bsp1407I	TGTACA	1 cut(s)	180
Bsp143I	GATC	1 cut(s)	147
BspACI	CCGC	4 cut(s)	52, 138, 372, 730
BspANI	GGCC	6 cut(s)	242, 275, 370, 638, 685, 744
BspCNI	CTCAG	1 cut(s)	301
BspLI	GGNNCC	6 cut(s)	67, 92, 93, 142, 176, 697
BspMAI	CTGCAG	1 cut(s)	561
BspQI	GCTCTTC	1 cut(s)	300
BspT107I	GGYRCC	3 cut(s)	65, 174, 695
BsrFI	RCCGGY	1 cut(s)	698
BsrGI	TGTACA	1 cut(s)	180
BssAI	RCCGGY	1 cut(s)	698
BssECI	CCNNGG	2 cut(s)	281, 792
BssMI	GATC	1 cut(s)	147
BssT1I	CCWWGG	2 cut(s)	281, 792
Bst4CI	ACNGT	2 cut(s)	290, 304
Bst6I	CTCTTC	1 cut(s)	300
BstAUI	TGTACA	1 cut(s)	180
BstC8I	GCNNGC	3 cut(s)	372, 561, 700
BstDEI	CTNAG	2 cut(s)	309, 336
BstF5I	GGATG	4 cut(s)	435, 666, 765, 774
BstH2I	RGCGCY	1 cut(s)	623
BstHHI	GCGC	2 cut(s)	126, 622
BstKTI	GATC	1 cut(s)	150
BstMAI	GTCTC	2 cut(s)	29, 163
BstMBI	GATC	1 cut(s)	147
BstMWI	GCNNNNNNNGC	2 cut(s)	272, 530
BstNSI	RCATGY	1 cut(s)	575
BstSFI	CTRYAG	1 cut(s)	557
BstV1I	GCAGC	1 cut(s)	108
BstXI	CCANNNNNNTGG	2 cut(s)	740, 760
BsuI	GTATCC	1 cut(s)	546
BsuRI	GGCC	6 cut(s)	242, 275, 370, 638, 685, 744
BtrI	CACGTC	1 cut(s)	117
BtsCI	GGATG	4 cut(s)	435, 666, 765, 774
BtsIMutI	CAGTG	1 cut(s)	295
Cac8I	GCNNGC	3 cut(s)	372, 561, 700
CfoI	GCGC	2 cut(s)	126, 622
Cfr10I	RCCGGY	1 cut(s)	698
Cfr13I	GGNCC	3 cut(s)	91, 141, 369
Csp6I	GTAC	4 cut(s)	181, 286, 313, 593
CviAII	CATG	9 cut(s)	244, 268, 277, 316, 446, 476, 572, 671, 691
CviQI	GTAC	4 cut(s)	181, 286, 313, 593
DdeI	CTNAG	2 cut(s)	309, 336
DpnI	GATC	1 cut(s)	149
DpnII	GATC	1 cut(s)	147
EaeI	YGGCCR	1 cut(s)	742
Eam1104I	CTCTTC	1 cut(s)	300
EarI	CTCTTC	1 cut(s)	300
Eco130I	CCWWGG	2 cut(s)	281, 792
Eco47I	GGWCC	2 cut(s)	91, 141
Eco47III	AGCGCT	1 cut(s)	621
EcoO109I	RGGNCCY	2 cut(s)	91, 141
EcoT14I	CCWWGG	2 cut(s)	281, 792
ErhI	CCWWGG	2 cut(s)	281, 792
Esp3I	CGTCTC	2 cut(s)	29, 163
FaeI	CATG	9 cut(s)	247, 271, 280, 319, 449, 479, 575, 674, 694
FaqI	GGGAC	3 cut(s)	59, 104, 154
FatI	CATG	9 cut(s)	243, 267, 276, 315, 445, 475, 571, 670, 690
FauI	CCCGC	2 cut(s)	131, 379
FauNDI	CATATG	2 cut(s)	641, 755
Fnu4HI	GCNGC	1 cut(s)	122
FokI	GGATG	4 cut(s)	442, 653, 772, 781
Fsp4HI	GCNGC	1 cut(s)	122
FspBI	CTAG	1 cut(s)	590
FspI	TGCGCA	1 cut(s)	125
GlaI	GCGC	2 cut(s)	125, 621
GluI	GCNGC	1 cut(s)	122
GsaI	CCCAGC	1 cut(s)	334
HaeII	RGCGCY	1 cut(s)	623
HaeIII	GGCC	6 cut(s)	242, 275, 370, 638, 685, 744
HapII	CCGG	2 cut(s)	162, 699
HhaI	GCGC	2 cut(s)	126, 622
Hin1II	CATG	9 cut(s)	247, 271, 280, 319, 449, 479, 575, 674, 694
Hin6I	GCGC	2 cut(s)	124, 620
HinP1I	GCGC	2 cut(s)	124, 620
HinfI	GANTC	3 cut(s)	8, 19, 803
HpaII	CCGG	2 cut(s)	162, 699
HphI	GGTGA	1 cut(s)	530
Hpy188I	TCNGA	2 cut(s)	310, 419
Hpy188III	TCNNGA	3 cut(s)	112, 659, 785
Hpy99I	CGWCG	2 cut(s)	43, 137
HpyAV	CCTTC	1 cut(s)	265
HpyCH4III	ACNGT	2 cut(s)	290, 304
HpyCH4IV	ACGT	4 cut(s)	41, 116, 132, 204
HpyCH4V	TGCA	6 cut(s)	253, 347, 433, 445, 559, 666
HpyF10VI	GCNNNNNNNGC	2 cut(s)	272, 530
HpyF3I	CTNAG	2 cut(s)	309, 336
HpySE526I	ACGT	4 cut(s)	41, 116, 132, 204
Hsp92II	CATG	9 cut(s)	247, 271, 280, 319, 449, 479, 575, 674, 694
HspAI	GCGC	2 cut(s)	124, 620
KflI	GGGWCCC	1 cut(s)	91
KroI	GCCGGC	1 cut(s)	698
KroNI	GCCGGC	1 cut(s)	700
Kzo9I	GATC	1 cut(s)	147
LguI	GCTCTTC	1 cut(s)	300
LmnI	GCTCC	1 cut(s)	627
Lsp1109I	GCAGC	1 cut(s)	108
LweI	GCATC	3 cut(s)	211, 420, 675
MaeI	CTAG	1 cut(s)	590
MaeII	ACGT	4 cut(s)	41, 116, 132, 204
MaeIII	GTNAC	1 cut(s)	785
MalI	GATC	1 cut(s)	149
MboI	GATC	1 cut(s)	147
MboII	GAAGA	4 cut(s)	19, 100, 287, 464
MhlI	GDGCHC	2 cut(s)	63, 295
MlsI	TGGCCA	1 cut(s)	744
MluCI	AATT	2 cut(s)	393, 420
MluNI	TGGCCA	1 cut(s)	744
MlyI	GAGTC	1 cut(s)	2
MmeI	TCCRAC	1 cut(s)	323
MnlI	CCTC	7 cut(s)	25, 164, 241, 389, 468, 521, 628
Mox20I	TGGCCA	1 cut(s)	744
MroNI	GCCGGC	1 cut(s)	698
MroXI	GAANNNNTTC	1 cut(s)	23
MscI	TGGCCA	1 cut(s)	744
MslI	CAYNNNNRTG	2 cut(s)	738, 758
Msp20I	TGGCCA	1 cut(s)	744
MspI	CCGG	2 cut(s)	162, 699
MwoI	GCNNNNNNNGC	2 cut(s)	272, 530
NaeI	GCCGGC	1 cut(s)	700
NdeI	CATATG	2 cut(s)	641, 755
NdeII	GATC	1 cut(s)	147
NgoMIV	GCCGGC	1 cut(s)	698
NlaIII	CATG	9 cut(s)	247, 271, 280, 319, 449, 479, 575, 674, 694
NlaIV	GGNNCC	6 cut(s)	67, 92, 93, 142, 176, 697
NmeAIII	GCCGAG	1 cut(s)	42
NmuCI	GTSAC	1 cut(s)	785
NsbI	TGCGCA	1 cut(s)	125
NspI	RCATGY	1 cut(s)	575
PciI	ACATGT	1 cut(s)	571
PciSI	GCTCTTC	1 cut(s)	300
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PdiI	GCCGGC	1 cut(s)	700
PdmI	GAANNNNTTC	1 cut(s)	23
PfeI	GAWTC	2 cut(s)	19, 803
PflMI	CCANNNNNTGG	2 cut(s)	282, 681
PkrI	GCNGC	1 cut(s)	123
PleI	GAGTC	1 cut(s)	2
PpsI	GAGTC	1 cut(s)	2
PpuMI	RGGWCCY	2 cut(s)	91, 141
PscI	ACATGT	1 cut(s)	571
Psp1406I	AACGTT	1 cut(s)	204
Psp5II	RGGWCCY	2 cut(s)	91, 141
PspFI	CCCAGC	1 cut(s)	330
PspN4I	GGNNCC	6 cut(s)	67, 92, 93, 142, 176, 697
PspPI	GGNCC	3 cut(s)	91, 141, 369
PspPPI	RGGWCCY	2 cut(s)	91, 141
PstI	CTGCAG	1 cut(s)	561
RsaI	GTAC	4 cut(s)	182, 287, 314, 594
RsaNI	GTAC	4 cut(s)	181, 286, 313, 593
RseI	CAYNNNNRTG	2 cut(s)	738, 758
SapI	GCTCTTC	1 cut(s)	300
SatI	GCNGC	1 cut(s)	122
Sau3AI	GATC	1 cut(s)	147
Sau96I	GGNCC	3 cut(s)	91, 141, 369
SchI	GAGTC	1 cut(s)	2
SduI	GDGCHC	2 cut(s)	63, 295
SfaNI	GCATC	3 cut(s)	211, 420, 675
SfcI	CTRYAG	1 cut(s)	557
SinI	GGWCC	2 cut(s)	91, 141
SmiMI	CAYNNNNRTG	2 cut(s)	738, 758
Sse9I	AATT	2 cut(s)	393, 420
SsiI	CCGC	4 cut(s)	52, 138, 372, 730
SspMI	CTAG	1 cut(s)	590
StyI	CCWWGG	2 cut(s)	281, 792
TaaI	ACNGT	2 cut(s)	290, 304
TaiI	ACGT	4 cut(s)	44, 119, 135, 207
TaqI	TCGA	2 cut(s)	30, 633
TasI	AATT	2 cut(s)	393, 420
TatI	WGTACW	2 cut(s)	180, 312
TfiI	GAWTC	2 cut(s)	19, 803
TscAI	CASTG	1 cut(s)	295
TseFI	GTSAC	1 cut(s)	785
TseI	GCWGC	1 cut(s)	121
Tsp45I	GTSAC	1 cut(s)	785
TspDTI	ATGAA	3 cut(s)	284, 464, 816
TspGWI	ACGGA	2 cut(s)	12, 507
TspRI	CASTG	1 cut(s)	295
Van91I	CCANNNNNTGG	2 cut(s)	282, 681
VpaK11BI	GGWCC	2 cut(s)	91, 141
XapI	RAATTY	1 cut(s)	420
XceI	RCATGY	1 cut(s)	575
XmnI	GAANNNNTTC	1 cut(s)	23
XspI	CTAG	1 cut(s)	590

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.