FvH4_2g26170

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0010864
Physical Location & Seq
Forward (+)
21063071 .. 21065848
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g26170.t1

Sequence Viewer

Length: 834 bp
ATGGAGTCGTCATCGCCGTCGTCATCGTACCTGAACCTGTTCCTGGAGGAGACGAGCTTCTACAACCGCATTGTGCTCGGGAGCCTGCTGCCCAAGTGGGTGTGGGCCCCGCTGCCACACTTCTTCCAGTCGTGGCTGCGTAACTACCTCGGCGGCACCTTAATCTACCTCGTCTCCGGCTTCCTCTGGTGCTTCTACATTTACTACCTCAAACGCAACGTTTATCTCCCCAAAGATGCCATTCCCTCTAATAAAGCCATGTTTTTGCAAATATATGTTGCCATGAAGGCCATGCCGTGGTACTGTGCTCTTCCGTCTGTCTCTGAGTACATGGTTGAGAATGGCTGGGCCAGGTGCTTCTCAAGAATAAGTGATGTAGGTTGGGTTGCATACCTCATCTATTTAGCAATTTATATGATTGTCGTCGAGTTTGGAATCTATTGGATGCACAGGGAGTTGCATGACATAAAGCCTCTGTATAAATGGCTACATAATATACATCACATCTACAACAAGCAAAATACCCTCTCTCCATTTGCTGGTTTGGCTTTCCACCCACTTGATGGGATACTGCAGGCAGTTCCGCATGTTATAGCTCTATTTCTTGTGCCAATGCATTTTACTACTCACATAGCGCTCCTATTTCTTGAAGCCATATGGACTGCAAACATTCATGATTGCATACATGCCAAAATATGGCCTGTGATGGGTGCTGGATACCACACCATTCATCATACTACGTATCGCCACAATTATGGACATTATACTATATGGATGGACTGGATGTTTGGAACTCTTCGTGACCCTATCAACGAGGAGTCTAAGGTCTTATGA

Protein Analysis

278

Amino Acids

32.65

Weight (kDa)

7.78

Isoelectric Point (pI)

58.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 136 - 265 5.4e-21 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02580 AT3G02590 AT3G02590
fragaria_vesca FvH4_2g26170
malus_domestica MD09G1254800.v1.1 MD17G1247400.v1.1
prunus_persica Prupe.3G144900_v2.0.a1
pyrus_communis pycom09g17070 pycom17g24880
rosa_chinensis RchiOBHm_Chr6g0294591
rosa_laevigata RLG00000011856
rosa_multiflora Rmu_sc0006264.1_g000012 Rmu_sc0007845.1_g000002
rosa_roxburghii Rroxscaffold_7G00173180
rosa_rugosa Rorug06G0246200
rosa_samantha Rh6AG358500 Rh6BG365700 Rh6CG372300 Rh6DG359100
rosa_wichuraiana Rw6G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 4 cut(s) 297, 539, 563, 696
AciI CCGC 4 cut(s) 67, 110, 153, 584
AclI AACGTT 1 cut(s) 219
AfaI GTAC 3 cut(s) 29, 302, 329
AfeI AGCGCT 1 cut(s) 636
AfiI CCNNNNNNNGG 6 cut(s) 43, 297, 539, 563, 696, 707
AgsI TTSAA 1 cut(s) 650
AjnI CCWGG 2 cut(s) 42, 350
AluBI AGCT 2 cut(s) 57, 596
AluI AGCT 2 cut(s) 57, 596
Alw21I GWGCWC 2 cut(s) 78, 310
Alw26I GTCTC 3 cut(s) 44, 178, 325
Ama87I CYCGRG 1 cut(s) 77
Aor51HI AGCGCT 1 cut(s) 636
AoxI GGCC 4 cut(s) 105, 288, 348, 698
ApaI GGGCCC 1 cut(s) 109
ApeKI GCWGC 3 cut(s) 88, 112, 136
ArsI GACNNNNNNTTYG 2 cut(s) 770, 802
Asp700I GAANNNNTTC 1 cut(s) 38
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 3 cut(s) 105, 106, 348
AvaI CYCGRG 1 cut(s) 77
BaeGI GKGCMC 1 cut(s) 109
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 109
Bbv12I GWGCWC 2 cut(s) 78, 310
BbvI GCAGC 3 cut(s) 75, 99, 123
BccI CCATC 3 cut(s) 557, 700, 769
BceAI ACGGC 1 cut(s) 280
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 2 cut(s) 44, 352
BciVI GTATCC 2 cut(s) 561, 710
BcoDI GTCTC 3 cut(s) 44, 178, 325
BfmI CTRYAG 1 cut(s) 572
BfoI RGCGCY 1 cut(s) 638
BfuI GTATCC 2 cut(s) 561, 710
BglI GCCNNNNNGGC 1 cut(s) 287
BisI GCNGC 4 cut(s) 89, 113, 137, 154
BlsI GCNGC 4 cut(s) 90, 114, 138, 155
Bme1390I CCNGG 2 cut(s) 44, 352
BmeT110I CYCGRG 1 cut(s) 77
BmgT120I GGNCC 3 cut(s) 105, 106, 348
BmiI GGNNCC 4 cut(s) 83, 107, 108, 157
BmrFI CCNGG 2 cut(s) 44, 352
BmsI GCATC 2 cut(s) 226, 435
BpmI CTGGAG 1 cut(s) 65
BpuEI CTTGAG 1 cut(s) 346
BsaAI YACGTR 1 cut(s) 741
BsaJI CCNNGG 2 cut(s) 148, 296
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 514, 544
Bsc4I CCNNNNNNNGG 6 cut(s) 43, 297, 539, 563, 696, 707
Bse1I ACTGG 2 cut(s) 127, 785
BseBI CCWGG 2 cut(s) 44, 352
BseDI CCNNGG 2 cut(s) 148, 296
BseGI GGATG 3 cut(s) 450, 780, 789
BseLI CCNNNNNNNGG 6 cut(s) 43, 297, 539, 563, 696, 707
BseMII CTCAG 1 cut(s) 315
BseNI ACTGG 2 cut(s) 127, 785
BseRI GAGGAG 2 cut(s) 62, 830
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 3 cut(s) 75, 99, 123
BseYI CCCAGC 1 cut(s) 345
BshFI GGCC 4 cut(s) 107, 290, 350, 700
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 78, 310
BsiHKCI CYCGRG 1 cut(s) 77
BsiSI CCGG 1 cut(s) 177
BslI CCNNNNNNNGG 6 cut(s) 43, 297, 539, 563, 696, 707
BsmAI GTCTC 3 cut(s) 44, 178, 325
BsmBI CGTCTC 2 cut(s) 44, 178
BsnI GGCC 4 cut(s) 107, 290, 350, 700
BsoBI CYCGRG 1 cut(s) 77
Bsp120I GGGCCC 1 cut(s) 105
Bsp1286I GDGCHC 3 cut(s) 78, 109, 310
BspACI CCGC 4 cut(s) 67, 110, 153, 584
BspANI GGCC 4 cut(s) 107, 290, 350, 700
BspCNI CTCAG 1 cut(s) 316
BspHI TCATGA 1 cut(s) 673
BspLI GGNNCC 4 cut(s) 83, 107, 108, 157
BspMAI CTGCAG 1 cut(s) 576
BspQI GCTCTTC 1 cut(s) 315
BspT107I GGYRCC 1 cut(s) 155
BsrI ACTGG 2 cut(s) 127, 785
BssECI CCNNGG 2 cut(s) 148, 296
Bst2UI CCWGG 2 cut(s) 44, 352
Bst4CI ACNGT 1 cut(s) 305
Bst6I CTCTTC 2 cut(s) 315, 801
BstBAI YACGTR 1 cut(s) 741
BstC8I GCNNGC 2 cut(s) 86, 576
BstDEI CTNAG 2 cut(s) 324, 822
BstDSI CCRYGG 1 cut(s) 296
BstF5I GGATG 3 cut(s) 450, 780, 789
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 1 cut(s) 637
BstMAI GTCTC 3 cut(s) 44, 178, 325
BstMWI GCNNNNNNNGC 2 cut(s) 287, 545
BstNI CCWGG 2 cut(s) 44, 352
BstNSI RCATGY 2 cut(s) 590, 689
BstSCI CCNGG 2 cut(s) 42, 350
BstSFI CTRYAG 1 cut(s) 572
BstSLI GKGCMC 1 cut(s) 109
BstSNI TACGTA 1 cut(s) 741
BstV1I GCAGC 3 cut(s) 75, 99, 123
BstXI CCANNNNNNTGG 1 cut(s) 755
BsuI GTATCC 2 cut(s) 561, 710
BsuRI GGCC 4 cut(s) 107, 290, 350, 700
BtgI CCRYGG 1 cut(s) 296
BtsCI GGATG 3 cut(s) 450, 780, 789
Cac8I GCNNGC 2 cut(s) 86, 576
CciI TCATGA 1 cut(s) 673
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 3 cut(s) 105, 106, 348
Csp6I GTAC 3 cut(s) 28, 301, 328
CviAII CATG 8 cut(s) 259, 283, 292, 331, 461, 587, 674, 686
CviQI GTAC 3 cut(s) 28, 301, 328
DdeI CTNAG 2 cut(s) 324, 822
Eam1104I CTCTTC 2 cut(s) 315, 801
EarI CTCTTC 2 cut(s) 315, 801
Eco105I TACGTA 1 cut(s) 741
Eco24I GRGCYC 1 cut(s) 109
Eco47III AGCGCT 1 cut(s) 636
Eco88I CYCGRG 1 cut(s) 77
EcoO109I RGGNCCY 1 cut(s) 106
EcoRII CCWGG 2 cut(s) 42, 350
EcoT22I ATGCAT 1 cut(s) 618
EcoT38I GRGCYC 1 cut(s) 109
Esp3I CGTCTC 2 cut(s) 44, 178
FaeI CATG 8 cut(s) 262, 286, 295, 334, 464, 590, 677, 689
FatI CATG 8 cut(s) 258, 282, 291, 330, 460, 586, 673, 685
FauI CCCGC 1 cut(s) 117
FauNDI CATATG 1 cut(s) 656
Fnu4HI GCNGC 4 cut(s) 89, 113, 137, 154
FokI GGATG 3 cut(s) 457, 787, 796
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 4 cut(s) 89, 113, 137, 154
GlaI GCGC 1 cut(s) 636
GluI GCNGC 4 cut(s) 89, 113, 137, 154
GsaI CCCAGC 1 cut(s) 349
GsuI CTGGAG 1 cut(s) 65
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 4 cut(s) 107, 290, 350, 700
HapII CCGG 1 cut(s) 177
HhaI GCGC 1 cut(s) 637
Hin1II CATG 8 cut(s) 262, 286, 295, 334, 464, 590, 677, 689
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HinfI GANTC 3 cut(s) 5, 435, 818
HpaII CCGG 1 cut(s) 177
Hpy188I TCNGA 1 cut(s) 325
Hpy188III TCNNGA 5 cut(s) 79, 363, 647, 674, 800
Hpy99I CGWCG 2 cut(s) 22, 428
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 2 cut(s) 219, 740
HpyCH4V TGCA 8 cut(s) 268, 389, 448, 460, 574, 616, 665, 681
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 545
HpyF3I CTNAG 2 cut(s) 324, 822
HpySE526I ACGT 2 cut(s) 219, 740
Hsp92II CATG 8 cut(s) 262, 286, 295, 334, 464, 590, 677, 689
HspAI GCGC 1 cut(s) 635
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 81, 642
Lsp1109I GCAGC 3 cut(s) 75, 99, 123
LweI GCATC 2 cut(s) 226, 435
MaeII ACGT 2 cut(s) 219, 740
MaeIII GTNAC 2 cut(s) 140, 800
MboII GAAGA 3 cut(s) 115, 302, 788
MhlI GDGCHC 3 cut(s) 78, 109, 310
MluCI AATT 2 cut(s) 408, 751
MlyI GAGTC 2 cut(s) 14, 827
Mph1103I ATGCAT 1 cut(s) 618
MroXI GAANNNNTTC 1 cut(s) 38
MseI TTAA 1 cut(s) 161
MslI CAYNNNNRTG 1 cut(s) 753
MspA1I CMGCKG 1 cut(s) 112
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 2 cut(s) 44, 352
MvaI CCWGG 2 cut(s) 44, 352
MwoI GCNNNNNNNGC 2 cut(s) 287, 545
NdeI CATATG 1 cut(s) 656
NlaIII CATG 8 cut(s) 262, 286, 295, 334, 464, 590, 677, 689
NlaIV GGNNCC 4 cut(s) 83, 107, 108, 157
NmeAIII GCCGAG 1 cut(s) 129
NmuCI GTSAC 1 cut(s) 800
NsiI ATGCAT 1 cut(s) 618
NspI RCATGY 2 cut(s) 590, 689
PagI TCATGA 1 cut(s) 673
PciSI GCTCTTC 1 cut(s) 315
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdmI GAANNNNTTC 1 cut(s) 38
PfeI GAWTC 1 cut(s) 435
PflMI CCANNNNNTGG 4 cut(s) 297, 539, 563, 696
PfoI TCCNGGA 1 cut(s) 42
PkrI GCNGC 4 cut(s) 90, 114, 138, 155
PleI GAGTC 2 cut(s) 13, 826
PpsI GAGTC 2 cut(s) 13, 826
Ppu21I YACGTR 1 cut(s) 741
Psp1406I AACGTT 1 cut(s) 219
Psp6I CCWGG 2 cut(s) 42, 350
PspFI CCCAGC 1 cut(s) 345
PspGI CCWGG 2 cut(s) 42, 350
PspN4I GGNNCC 4 cut(s) 83, 107, 108, 157
PspOMI GGGCCC 1 cut(s) 105
PspPI GGNCC 3 cut(s) 105, 106, 348
PstI CTGCAG 1 cut(s) 576
RsaI GTAC 3 cut(s) 29, 302, 329
RsaNI GTAC 3 cut(s) 28, 301, 328
RseI CAYNNNNRTG 1 cut(s) 753
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 1 cut(s) 161
SatI GCNGC 4 cut(s) 89, 113, 137, 154
Sau96I GGNCC 3 cut(s) 105, 106, 348
SchI GAGTC 2 cut(s) 14, 827
ScrFI CCNGG 2 cut(s) 44, 352
SduI GDGCHC 3 cut(s) 78, 109, 310
SfaNI GCATC 2 cut(s) 226, 435
SfcI CTRYAG 1 cut(s) 572
SmiMI CAYNNNNRTG 1 cut(s) 753
SmlI CTYRAG 1 cut(s) 361
SmoI CTYRAG 1 cut(s) 361
SnaBI TACGTA 1 cut(s) 741
Sse9I AATT 2 cut(s) 408, 751
SsiI CCGC 4 cut(s) 67, 110, 153, 584
StyD4I CCNGG 2 cut(s) 42, 350
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 2 cut(s) 222, 743
TaqI TCGA 1 cut(s) 426
TasI AATT 2 cut(s) 408, 751
TatI WGTACW 1 cut(s) 327
TauI GCSGC 1 cut(s) 156
TfiI GAWTC 1 cut(s) 435
Tru1I TTAA 1 cut(s) 161
Tru9I TTAA 1 cut(s) 161
TseFI GTSAC 1 cut(s) 800
TseI GCWGC 3 cut(s) 88, 112, 136
Tsp45I GTSAC 1 cut(s) 800
TspDTI ATGAA 3 cut(s) 299, 662, 719
TspGWI ACGGA 1 cut(s) 303
Van91I CCANNNNNTGG 4 cut(s) 297, 539, 563, 696
XceI RCATGY 2 cut(s) 590, 689
XcmI CCANNNNNNNNNTGG 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 618
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.