Rroxscaffold_7G00173180

Belongs to the sterol desaturase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0010864
Physical Location & Seq
Reverse (-)
13289415 .. 13291801
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00173180.1

Sequence Viewer

Length: 831 bp
ATGGAGTCCTCGTCGTCGTCGTCGTACCTGAACTTGTTCGTGGAGGAGACAAGCTTCTATAACCGCATTGTCCTCGACAGCCTGATGCCCAACTGGGTGTGGGCCCCGCTGCCCCACTTCTTCCAGTCGTGGCTGCGCAACTACCTCGGCGGCACCTTAATCTACCTCGTCTCCGGCTTCCTCTGGTGCTTCTACATTTACTACCTCAAACGCAACGTCTACCTTCCAAAAGATGCCATTCCCTCTAATAAAGCCATGCTTTTGCAAATATATGTTGCCATGAAGGCCATGCCTTGGTACTGTGCTCTTCCGTCTCTCTCTGAGTACATGGTTGAAAATGGCTGGGCGAGGTGCTTCTCAAGAATAAGTGATGTAGGTTGGGTTGCTTACCTCATCTATTTATCAATTTATCTTATAATCGTGGAGTTTGGAATCTATTGGATGCATAGAGAGTTGCATGACATAAAGCCTCTGTATAAATGGCTTCATAATATACATCACATTTACAACAAGCAGAACACCCTCTCTCCATTTGCTGGTTTGGCTTTCCACCCACTTGATGGGATACTGCAGGCAGTTCCGCATGTTATAGCTCTGTTTCTTGTGCCAATGCATTTTACTACTCACATAGCGCTCCTATTTATTGAAGCCATATGGACAGCAAATATTCACGATTGCATCCATGCCAAAATATGGCCTGTGATGGGTGCTGGCTACCACACCATTCATCATACTACCTATCGCCATAATTATGGCCATTATACTATATGGATGGACTGGATGTTTGGAACTCTTCGTGACCCTATCAATGAGGAATCAAAGGTCTTATGA

Protein Analysis

276

Amino Acids

32.59

Weight (kDa)

6.99

Isoelectric Point (pI)

58.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_hydroxylase PF04116 136 - 264 7e-21 Fatty acid hydroxylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010864)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02580 AT3G02590 AT3G02590
fragaria_vesca FvH4_2g26170
malus_domestica MD09G1254800.v1.1 MD17G1247400.v1.1
prunus_persica Prupe.3G144900_v2.0.a1
pyrus_communis pycom09g17070 pycom17g24880
rosa_chinensis RchiOBHm_Chr6g0294591
rosa_laevigata RLG00000011856
rosa_multiflora Rmu_sc0006264.1_g000012 Rmu_sc0007845.1_g000002
rosa_roxburghii Rroxscaffold_7G00173180
rosa_rugosa Rorug06G0246200
rosa_samantha Rh6AG358500 Rh6BG365700 Rh6CG372300 Rh6DG359100
rosa_wichuraiana Rw6G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 416
Acc16I TGCGCA 1 cut(s) 137
AccB1I GGYRCC 1 cut(s) 152
AccB7I CCANNNNNTGG 4 cut(s) 294, 536, 560, 693
AccI GTMKAC 1 cut(s) 219
AciI CCGC 4 cut(s) 64, 107, 150, 581
AcoI YGGCCR 1 cut(s) 754
AfaI GTAC 3 cut(s) 26, 299, 326
AfeI AGCGCT 1 cut(s) 633
AfiI CCNNNNNNNGG 5 cut(s) 294, 536, 560, 693, 704
AgsI TTSAA 2 cut(s) 335, 647
AluBI AGCT 2 cut(s) 54, 593
AluI AGCT 2 cut(s) 54, 593
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 3 cut(s) 41, 175, 318
Aor51HI AGCGCT 1 cut(s) 633
AoxI GGCC 4 cut(s) 102, 285, 695, 754
ApaI GGGCCC 1 cut(s) 106
ApeKI GCWGC 2 cut(s) 109, 133
ArsI GACNNNNNNTTYG 4 cut(s) 201, 233, 767, 799
Asp700I GAANNNNTTC 1 cut(s) 35
AspLEI GCGC 2 cut(s) 138, 634
AspS9I GGNCC 2 cut(s) 102, 103
BaeGI GKGCMC 1 cut(s) 106
BalI TGGCCA 1 cut(s) 756
BanI GGYRCC 1 cut(s) 152
BanII GRGCYC 1 cut(s) 106
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 2 cut(s) 96, 120
BccI CCATC 3 cut(s) 554, 697, 766
BcgI CGANNNNNNTGC 4 cut(s) 55, 89, 127, 161
BciVI GTATCC 1 cut(s) 558
BcoDI GTCTC 3 cut(s) 41, 175, 318
BfmI CTRYAG 1 cut(s) 569
BfoI RGCGCY 1 cut(s) 635
BfuI GTATCC 1 cut(s) 558
BglI GCCNNNNNGGC 1 cut(s) 284
BisI GCNGC 3 cut(s) 110, 134, 151
BlsI GCNGC 3 cut(s) 111, 135, 152
BmgT120I GGNCC 2 cut(s) 102, 103
BmiI GGNNCC 3 cut(s) 104, 105, 154
BmrI ACTGGG 1 cut(s) 103
BmsI GCATC 4 cut(s) 75, 223, 432, 687
BmuI ACTGGG 1 cut(s) 103
BpuEI CTTGAG 1 cut(s) 343
BsaJI CCNNGG 2 cut(s) 145, 293
BsaXI ACNNNNNCTCC 5 cut(s) 26, 155, 185, 511, 541
Bsc4I CCNNNNNNNGG 5 cut(s) 294, 536, 560, 693, 704
Bse1I ACTGG 3 cut(s) 98, 124, 782
BseDI CCNNGG 2 cut(s) 145, 293
BseGI GGATG 4 cut(s) 447, 678, 777, 786
BseLI CCNNNNNNNGG 5 cut(s) 294, 536, 560, 693, 704
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 3 cut(s) 98, 124, 782
BseRI GAGGAG 1 cut(s) 59
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 2 cut(s) 96, 120
BseYI CCCAGC 1 cut(s) 342
BshFI GGCC 4 cut(s) 104, 287, 697, 756
BshNI GGYRCC 1 cut(s) 152
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 174
BslI CCNNNNNNNGG 5 cut(s) 294, 536, 560, 693, 704
BsmAI GTCTC 3 cut(s) 41, 175, 318
BsmBI CGTCTC 2 cut(s) 175, 318
BsnI GGCC 4 cut(s) 104, 287, 697, 756
Bsp120I GGGCCC 1 cut(s) 102
Bsp1286I GDGCHC 2 cut(s) 106, 307
BspACI CCGC 4 cut(s) 64, 107, 150, 581
BspANI GGCC 4 cut(s) 104, 287, 697, 756
BspCNI CTCAG 1 cut(s) 313
BspLI GGNNCC 3 cut(s) 104, 105, 154
BspMAI CTGCAG 1 cut(s) 573
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 152
BsrI ACTGG 3 cut(s) 98, 124, 782
BssECI CCNNGG 2 cut(s) 145, 293
BssT1I CCWWGG 1 cut(s) 293
Bst4CI ACNGT 1 cut(s) 302
Bst6I CTCTTC 2 cut(s) 312, 798
BstC8I GCNNGC 2 cut(s) 573, 712
BstDEI CTNAG 1 cut(s) 321
BstF5I GGATG 4 cut(s) 447, 678, 777, 786
BstH2I RGCGCY 1 cut(s) 635
BstHHI GCGC 2 cut(s) 138, 634
BstMAI GTCTC 3 cut(s) 41, 175, 318
BstMWI GCNNNNNNNGC 2 cut(s) 284, 542
BstNSI RCATGY 1 cut(s) 587
BstSFI CTRYAG 1 cut(s) 569
BstSLI GKGCMC 1 cut(s) 106
BstV1I GCAGC 2 cut(s) 96, 120
BstXI CCANNNNNNTGG 1 cut(s) 752
BsuI GTATCC 1 cut(s) 558
BsuRI GGCC 4 cut(s) 104, 287, 697, 756
BtsCI GGATG 4 cut(s) 447, 678, 777, 786
Cac8I GCNNGC 2 cut(s) 573, 712
CfoI GCGC 2 cut(s) 138, 634
Cfr13I GGNCC 2 cut(s) 102, 103
Csp6I GTAC 3 cut(s) 25, 298, 325
CviAII CATG 7 cut(s) 256, 280, 289, 328, 458, 584, 683
CviQI GTAC 3 cut(s) 25, 298, 325
DdeI CTNAG 1 cut(s) 321
EaeI YGGCCR 1 cut(s) 754
Eam1104I CTCTTC 2 cut(s) 312, 798
EarI CTCTTC 2 cut(s) 312, 798
Eco130I CCWWGG 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 106
Eco47III AGCGCT 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 293
EcoT22I ATGCAT 2 cut(s) 447, 615
EcoT38I GRGCYC 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 293
Esp3I CGTCTC 2 cut(s) 175, 318
FaeI CATG 7 cut(s) 259, 283, 292, 331, 461, 587, 686
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FatI CATG 7 cut(s) 255, 279, 288, 327, 457, 583, 682
FauI CCCGC 1 cut(s) 114
FauNDI CATATG 1 cut(s) 653
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 3 cut(s) 110, 134, 151
FokI GGATG 4 cut(s) 454, 665, 784, 793
FriOI GRGCYC 1 cut(s) 106
Fsp4HI GCNGC 3 cut(s) 110, 134, 151
FspI TGCGCA 1 cut(s) 137
GlaI GCGC 2 cut(s) 137, 633
GluI GCNGC 3 cut(s) 110, 134, 151
GsaI CCCAGC 1 cut(s) 346
HaeII RGCGCY 1 cut(s) 635
HaeIII GGCC 4 cut(s) 104, 287, 697, 756
HapII CCGG 1 cut(s) 174
HhaI GCGC 2 cut(s) 138, 634
Hin1II CATG 7 cut(s) 259, 283, 292, 331, 461, 587, 686
Hin6I GCGC 2 cut(s) 136, 632
HinP1I GCGC 2 cut(s) 136, 632
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 3 cut(s) 5, 432, 815
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 1 cut(s) 220
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 3 cut(s) 360, 671, 797
Hpy8I GTNNAC 1 cut(s) 220
Hpy99I CGWCG 4 cut(s) 16, 19, 22, 25
HpyAV CCTTC 2 cut(s) 233, 277
HpyCH4III ACNGT 1 cut(s) 302
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 6 cut(s) 265, 445, 457, 571, 613, 678
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 542
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 7 cut(s) 259, 283, 292, 331, 461, 587, 686
HspAI GCGC 2 cut(s) 136, 632
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 1 cut(s) 639
Lsp1109I GCAGC 2 cut(s) 96, 120
LweI GCATC 4 cut(s) 75, 223, 432, 687
MaeII ACGT 1 cut(s) 216
MaeIII GTNAC 1 cut(s) 797
MboII GAAGA 3 cut(s) 112, 299, 785
MhlI GDGCHC 2 cut(s) 106, 307
MlsI TGGCCA 1 cut(s) 756
MluCI AATT 2 cut(s) 405, 748
MluNI TGGCCA 1 cut(s) 756
MlyI GAGTC 1 cut(s) 14
Mox20I TGGCCA 1 cut(s) 756
Mph1103I ATGCAT 2 cut(s) 447, 615
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 756
MseI TTAA 1 cut(s) 158
MslI CAYNNNNRTG 1 cut(s) 750
Msp20I TGGCCA 1 cut(s) 756
MspA1I CMGCKG 1 cut(s) 109
MspI CCGG 1 cut(s) 174
MwoI GCNNNNNNNGC 2 cut(s) 284, 542
NdeI CATATG 1 cut(s) 653
NlaIII CATG 7 cut(s) 259, 283, 292, 331, 461, 587, 686
NlaIV GGNNCC 3 cut(s) 104, 105, 154
NmeAIII GCCGAG 1 cut(s) 126
NmuCI GTSAC 1 cut(s) 797
NsbI TGCGCA 1 cut(s) 137
NsiI ATGCAT 2 cut(s) 447, 615
NspI RCATGY 1 cut(s) 587
PciSI GCTCTTC 1 cut(s) 312
PcsI WCGNNNNNNNCGW 2 cut(s) 17, 20
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 2 cut(s) 432, 815
PflMI CCANNNNNTGG 4 cut(s) 294, 536, 560, 693
PkrI GCNGC 3 cut(s) 111, 135, 152
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PsiI TTATAA 1 cut(s) 416
PspFI CCCAGC 1 cut(s) 342
PspN4I GGNNCC 3 cut(s) 104, 105, 154
PspOMI GGGCCC 1 cut(s) 102
PspPI GGNCC 2 cut(s) 102, 103
PstI CTGCAG 1 cut(s) 573
RsaI GTAC 3 cut(s) 26, 299, 326
RsaNI GTAC 3 cut(s) 25, 298, 325
RseI CAYNNNNRTG 1 cut(s) 750
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 1 cut(s) 158
SatI GCNGC 3 cut(s) 110, 134, 151
Sau96I GGNCC 2 cut(s) 102, 103
SchI GAGTC 1 cut(s) 14
SduI GDGCHC 2 cut(s) 106, 307
SfaNI GCATC 4 cut(s) 75, 223, 432, 687
SfcI CTRYAG 1 cut(s) 569
SmiMI CAYNNNNRTG 1 cut(s) 750
SmlI CTYRAG 1 cut(s) 358
SmoI CTYRAG 1 cut(s) 358
Sse9I AATT 2 cut(s) 405, 748
SsiI CCGC 4 cut(s) 64, 107, 150, 581
SspI AATATT 1 cut(s) 667
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 1 cut(s) 302
TaiI ACGT 1 cut(s) 219
TaqI TCGA 1 cut(s) 75
TasI AATT 2 cut(s) 405, 748
TatI WGTACW 1 cut(s) 324
TauI GCSGC 1 cut(s) 153
TfiI GAWTC 2 cut(s) 432, 815
Tru1I TTAA 1 cut(s) 158
Tru9I TTAA 1 cut(s) 158
TseFI GTSAC 1 cut(s) 797
TseI GCWGC 2 cut(s) 109, 133
Tsp45I GTSAC 1 cut(s) 797
TspDTI ATGAA 3 cut(s) 296, 476, 716
TspGWI ACGGA 1 cut(s) 300
Van91I CCANNNNNTGG 4 cut(s) 294, 536, 560, 693
XceI RCATGY 1 cut(s) 587
XcmI CCANNNNNNNNNTGG 1 cut(s) 557
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 35
Zsp2I ATGCAT 2 cut(s) 447, 615
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.