AT3G03180

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004920
Physical Location & Seq
Forward (+)
733253 .. 734662
1410 bp
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UTR
Exon/CDS
Intron
AT3G03180.3

Sequence Viewer

Length: 423 bp
ATGGCTTCCTTGGAGATGAATGACCTAAAAAAGATAGGGCTAGGTTTGACTGGATTTGGTGTGTTCTTTACATTCCTGGGAGTGATATTTGTGTTTGACAAGGGACTCATAGCCATGGGGAACATCCTCTTCTTAGCTGGTGTGACTCTAACCATTGGAATCAACCCAGCAATCCAATTCTTTACTAAACGGCAGAATTTTAAGGGTACTATATCATTTGGATTGGGTTTCTTGTTGGTTGTGTTTGGATGGCCTATTTTCGGTTTGCTTCTTGAATCATATGGGTTCCTTGTGCTCTTCAGTGGTTTCTGGCCTACACTTGCCGTCTTCCTTCAAAGGATACCTCTATTAGGCTGGCTTTTGCAGCAACCATACATCAGATCGTTGCTGGATCGCTACCGTGGAAGGCGTGTCCCTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Got1

Protein Analysis

140

Amino Acids

15.72

Weight (kDa)

10.43

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 16 - 115 3.8e-12 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 399
AcsI RAATTY 1 cut(s) 196
AcuI CTGAAG 1 cut(s) 283
AfaI GTAC 1 cut(s) 208
AfiI CCNNNNNNNGG 2 cut(s) 260, 350
AgsI TTSAA 2 cut(s) 275, 335
AjnI CCWGG 1 cut(s) 75
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Alw21I GWGCWC 1 cut(s) 297
AlwI GGATC 1 cut(s) 399
AoxI GGCC 2 cut(s) 251, 311
ApeKI GCWGC 1 cut(s) 364
ApoI RAATTY 1 cut(s) 196
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 297
BbvI GCAGC 1 cut(s) 376
BccI CCATC 1 cut(s) 243
BceAI ACGGC 2 cut(s) 206, 308
BciT130I CCWGG 1 cut(s) 77
BciVI GTATCC 1 cut(s) 333
BfaI CTAG 1 cut(s) 41
BfuI GTATCC 1 cut(s) 333
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 287
BmrFI CCNGG 1 cut(s) 77
BpiI GAAGAC 1 cut(s) 319
BsaJI CCNNGG 4 cut(s) 9, 76, 114, 400
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 350
Bse1I ACTGG 1 cut(s) 55
BseBI CCWGG 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 9, 76, 114, 400
BseGI GGATG 2 cut(s) 123, 254
BseLI CCNNNNNNNGG 2 cut(s) 260, 350
BseNI ACTGG 1 cut(s) 55
BseXI GCAGC 1 cut(s) 376
BseYI CCCAGC 1 cut(s) 166
BshFI GGCC 2 cut(s) 253, 313
BsiHKAI GWGCWC 1 cut(s) 297
BslFI GGGAC 2 cut(s) 117, 398
BslI CCNNNNNNNGG 2 cut(s) 260, 350
BsmFI GGGAC 2 cut(s) 117, 398
BsnI GGCC 2 cut(s) 253, 313
Bsp1286I GDGCHC 1 cut(s) 297
Bsp143I GATC 2 cut(s) 380, 391
Bsp19I CCATGG 1 cut(s) 114
BspANI GGCC 2 cut(s) 253, 313
BspLI GGNNCC 1 cut(s) 287
BspPI GGATC 1 cut(s) 399
BspQI GCTCTTC 1 cut(s) 302
BsrI ACTGG 1 cut(s) 55
BssECI CCNNGG 4 cut(s) 9, 76, 114, 400
BssMI GATC 2 cut(s) 380, 391
BssT1I CCWWGG 2 cut(s) 9, 114
Bst2UI CCWGG 1 cut(s) 77
Bst4CI ACNGT 1 cut(s) 401
Bst6I CTCTTC 2 cut(s) 134, 302
BstC8I GCNNGC 1 cut(s) 356
BstDEI CTNAG 1 cut(s) 133
BstDSI CCRYGG 2 cut(s) 114, 400
BstENI CCTNNNNNAGG 1 cut(s) 348
BstF5I GGATG 2 cut(s) 123, 254
BstKTI GATC 2 cut(s) 383, 394
BstMBI GATC 2 cut(s) 380, 391
BstMWI GCNNNNNNNGC 1 cut(s) 364
BstNI CCWGG 1 cut(s) 77
BstSCI CCNGG 1 cut(s) 75
BstV1I GCAGC 1 cut(s) 376
BstV2I GAAGAC 1 cut(s) 319
BsuI GTATCC 1 cut(s) 333
BsuRI GGCC 2 cut(s) 253, 313
BtgI CCRYGG 2 cut(s) 114, 400
BtsCI GGATG 2 cut(s) 123, 254
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 1 cut(s) 356
Csp6I GTAC 1 cut(s) 207
CviAII CATG 1 cut(s) 115
CviJI RGCY 8 cut(s) 5, 40, 113, 137, 253, 313, 354, 358
CviKI_1 RGCY 8 cut(s) 5, 40, 113, 137, 253, 313, 354, 358
CviQI GTAC 1 cut(s) 207
DdeI CTNAG 1 cut(s) 133
DpnI GATC 2 cut(s) 382, 393
DpnII GATC 2 cut(s) 380, 391
Eam1104I CTCTTC 2 cut(s) 134, 302
EarI CTCTTC 2 cut(s) 134, 302
Eco130I CCWWGG 2 cut(s) 9, 114
Eco57I CTGAAG 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 348
EcoRII CCWGG 1 cut(s) 75
EcoT14I CCWWGG 2 cut(s) 9, 114
ErhI CCWWGG 2 cut(s) 9, 114
FaeI CATG 1 cut(s) 118
FaiI YATR 6 cut(s) 110, 116, 212, 280, 282, 373
FaqI GGGAC 2 cut(s) 117, 398
FatI CATG 1 cut(s) 114
FauNDI CATATG 1 cut(s) 280
Fnu4HI GCNGC 1 cut(s) 365
FokI GGATG 2 cut(s) 110, 261
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 1 cut(s) 41
GluI GCNGC 1 cut(s) 365
GsaI CCCAGC 1 cut(s) 170
HaeIII GGCC 2 cut(s) 253, 313
Hin1II CATG 1 cut(s) 118
HinfI GANTC 4 cut(s) 105, 145, 159, 275
Hpy188I TCNGA 1 cut(s) 380
Hpy188III TCNNGA 1 cut(s) 272
HpyAV CCTTC 2 cut(s) 341, 399
HpyCH4III ACNGT 1 cut(s) 401
HpyCH4V TGCA 1 cut(s) 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 364
HpyF3I CTNAG 1 cut(s) 133
Hsp92II CATG 1 cut(s) 118
Kzo9I GATC 2 cut(s) 380, 391
LguI GCTCTTC 1 cut(s) 302
LpnPI CCDG 8 cut(s) 36, 62, 89, 123, 180, 295, 340, 374
Lsp1109I GCAGC 1 cut(s) 376
MaeI CTAG 1 cut(s) 41
MaeIII GTNAC 1 cut(s) 142
MalI GATC 2 cut(s) 382, 393
MboI GATC 2 cut(s) 380, 391
MboII GAAGA 3 cut(s) 121, 289, 319
MhlI GDGCHC 1 cut(s) 297
MluCI AATT 2 cut(s) 176, 196
MlyI GAGTC 2 cut(s) 99, 139
MnlI CCTC 2 cut(s) 137, 354
MseI TTAA 1 cut(s) 201
MslI CAYNNNNRTG 1 cut(s) 113
MspR9I CCNGG 1 cut(s) 77
MvaI CCWGG 1 cut(s) 77
MwoI GCNNNNNNNGC 1 cut(s) 364
NcoI CCATGG 1 cut(s) 114
NdeI CATATG 1 cut(s) 280
NdeII GATC 2 cut(s) 380, 391
NlaIII CATG 1 cut(s) 118
NlaIV GGNNCC 1 cut(s) 287
NmuCI GTSAC 1 cut(s) 142
PciSI GCTCTTC 1 cut(s) 302
PfeI GAWTC 2 cut(s) 159, 275
PkrI GCNGC 1 cut(s) 366
PleI GAGTC 2 cut(s) 99, 139
PpsI GAGTC 2 cut(s) 99, 139
Psp6I CCWGG 1 cut(s) 75
PspFI CCCAGC 1 cut(s) 166
PspGI CCWGG 1 cut(s) 75
PspN4I GGNNCC 1 cut(s) 287
RsaI GTAC 1 cut(s) 208
RsaNI GTAC 1 cut(s) 207
RseI CAYNNNNRTG 1 cut(s) 113
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 201
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 2 cut(s) 380, 391
SchI GAGTC 2 cut(s) 99, 139
ScrFI CCNGG 1 cut(s) 77
SduI GDGCHC 1 cut(s) 297
SetI ASST 4 cut(s) 27, 46, 139, 346
SmiMI CAYNNNNRTG 1 cut(s) 113
Sse9I AATT 2 cut(s) 176, 196
SspMI CTAG 1 cut(s) 41
StyD4I CCNGG 1 cut(s) 75
StyI CCWWGG 2 cut(s) 9, 114
TaaI ACNGT 1 cut(s) 401
TasI AATT 2 cut(s) 176, 196
TfiI GAWTC 2 cut(s) 159, 275
Tru1I TTAA 1 cut(s) 201
Tru9I TTAA 1 cut(s) 201
TscAI CASTG 1 cut(s) 307
TseFI GTSAC 1 cut(s) 142
TseI GCWGC 1 cut(s) 364
Tsp45I GTSAC 1 cut(s) 142
TspDTI ATGAA 1 cut(s) 32
TspRI CASTG 1 cut(s) 307
XagI CCTNNNNNAGG 1 cut(s) 348
XapI RAATTY 1 cut(s) 196
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.