MD13G1259200.v1.1

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004920
Physical Location & Seq
Reverse (-)
31320848 .. 31323184
2337 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1259200.v1.1.491

Sequence Viewer

Length: 438 bp
ATGTCAGCTAGCCTTACCCTATCTTATGGAGAGGCTGCTAATTTCAAGATTGGACTCGGATTGACTGGATTCGGTATATTTTTCACATGCCTGGGAATGGCCTTCTTCTTTGACAAGGGATTGCTTGCAATGGGAAATATCCTCTTCTTGTCAGGGGTGACTTTAACCATTGGATTTAAGCCCACGATGCAATTCTTCATGAAACGTCAAAATTATAAGGGAACTATTTCTTTTGGTATTGGTTTCTTCTTTGTGATCATAGGGTGGCCTATTATTGGCATGATTTTGGAGGCATATGGGTTCATCGTACTCTTCAGCGGCTTCTGGCCAACACTGGCAGTTTTTCTTCAGAAGATTCCGGTTCTTGGCTGGCTGTTTCAACAACCATACGTTAGATCACTCTTTGACCGATATCGTGGCCGACGGGTGCCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Got1

Protein Analysis

146

Amino Acids

16.32

Weight (kDa)

10.08

Isoelectric Point (pI)

28.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Got1 PF04178 21 - 120 9.3e-13 Got1/Sft2-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 216
AccB1I GGYRCC 1 cut(s) 427
AciI CCGC 1 cut(s) 318
AcoI YGGCCR 2 cut(s) 326, 418
AcuI CTGAAG 2 cut(s) 298, 332
AfaI GTAC 1 cut(s) 309
AfiI CCNNNNNNNGG 3 cut(s) 97, 275, 365
AgsI TTSAA 2 cut(s) 46, 380
AjnI CCWGG 1 cut(s) 90
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
AoxI GGCC 4 cut(s) 99, 266, 326, 418
ApeKI GCWGC 1 cut(s) 35
Asp700I GAANNNNTTC 1 cut(s) 226
AsuHPI GGTGA 1 cut(s) 169
AsuNHI GCTAGC 1 cut(s) 8
BalI TGGCCA 1 cut(s) 328
BanI GGYRCC 1 cut(s) 427
BbvI GCAGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 92
BclI TGATCA 1 cut(s) 255
BfaI CTAG 1 cut(s) 9
BisI GCNGC 2 cut(s) 36, 319
BlsI GCNGC 2 cut(s) 37, 320
Bme1390I CCNGG 1 cut(s) 92
BmiI GGNNCC 1 cut(s) 429
BmrFI CCNGG 1 cut(s) 92
BmsI GCATC 1 cut(s) 177
BmtI GCTAGC 1 cut(s) 12
BsaJI CCNNGG 1 cut(s) 91
BsaWI WCCGGW 1 cut(s) 358
Bsc4I CCNNNNNNNGG 3 cut(s) 97, 275, 365
Bse1I ACTGG 2 cut(s) 70, 339
Bse3DI GCAATG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 91
BseLI CCNNNNNNNGG 3 cut(s) 97, 275, 365
BseMI GCAATG 1 cut(s) 135
BseNI ACTGG 2 cut(s) 70, 339
BseXI GCAGC 1 cut(s) 22
BshFI GGCC 4 cut(s) 101, 268, 328, 420
BshNI GGYRCC 1 cut(s) 427
BsiSI CCGG 1 cut(s) 359
BslI CCNNNNNNNGG 3 cut(s) 97, 275, 365
BsnI GGCC 4 cut(s) 101, 268, 328, 420
Bsp143I GATC 2 cut(s) 255, 395
BspACI CCGC 1 cut(s) 318
BspANI GGCC 4 cut(s) 101, 268, 328, 420
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 429
BspOI GCTAGC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 427
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 2 cut(s) 70, 339
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 2 cut(s) 255, 395
Bst2UI CCWGG 1 cut(s) 92
Bst6I CTCTTC 2 cut(s) 149, 317
BstC8I GCNNGC 3 cut(s) 10, 126, 371
BstKTI GATC 2 cut(s) 258, 398
BstMBI GATC 2 cut(s) 255, 395
BstMWI GCNNNNNNNGC 1 cut(s) 187
BstNI CCWGG 1 cut(s) 92
BstNSI RCATGY 1 cut(s) 90
BstSCI CCNGG 1 cut(s) 90
BstV1I GCAGC 1 cut(s) 22
BsuRI GGCC 4 cut(s) 101, 268, 328, 420
BtsIMutI CAGTG 1 cut(s) 332
Cac8I GCNNGC 3 cut(s) 10, 126, 371
CciI TCATGA 1 cut(s) 198
Csp6I GTAC 1 cut(s) 308
CviAII CATG 3 cut(s) 87, 199, 280
CviQI GTAC 1 cut(s) 308
DpnI GATC 2 cut(s) 257, 397
DpnII GATC 2 cut(s) 255, 395
EaeI YGGCCR 2 cut(s) 326, 418
Eam1104I CTCTTC 2 cut(s) 149, 317
EarI CTCTTC 2 cut(s) 149, 317
Eco32I GATATC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 298, 332
EcoRII CCWGG 1 cut(s) 90
EcoRV GATATC 1 cut(s) 413
FaeI CATG 3 cut(s) 90, 202, 283
FatI CATG 3 cut(s) 86, 198, 279
FauNDI CATATG 1 cut(s) 295
FbaI TGATCA 1 cut(s) 255
Fnu4HI GCNGC 2 cut(s) 36, 319
Fsp4HI GCNGC 2 cut(s) 36, 319
FspBI CTAG 1 cut(s) 9
GluI GCNGC 2 cut(s) 36, 319
HaeIII GGCC 4 cut(s) 101, 268, 328, 420
HapII CCGG 1 cut(s) 359
Hin1II CATG 3 cut(s) 90, 202, 283
HinfI GANTC 3 cut(s) 54, 69, 355
HpaII CCGG 1 cut(s) 359
HphI GGTGA 1 cut(s) 169
Hpy188I TCNGA 2 cut(s) 59, 351
Hpy188III TCNNGA 2 cut(s) 46, 199
Hpy99I CGWCG 1 cut(s) 426
HpyAV CCTTC 1 cut(s) 112
HpyCH4IV ACGT 2 cut(s) 205, 390
HpyCH4V TGCA 2 cut(s) 128, 190
HpyF10VI GCNNNNNNNGC 1 cut(s) 187
HpySE526I ACGT 2 cut(s) 205, 390
Hsp92II CATG 3 cut(s) 90, 202, 283
Ksp22I TGATCA 1 cut(s) 255
Kzo9I GATC 2 cut(s) 255, 395
LpnPI CCDG 8 cut(s) 51, 77, 104, 138, 310, 320, 355, 372
Lsp1109I GCAGC 1 cut(s) 22
LweI GCATC 1 cut(s) 177
MaeI CTAG 1 cut(s) 9
MaeII ACGT 2 cut(s) 205, 390
MaeIII GTNAC 1 cut(s) 157
MalI GATC 2 cut(s) 257, 397
MboI GATC 2 cut(s) 255, 395
MboII GAAGA 7 cut(s) 97, 136, 187, 238, 304, 338, 364
MlsI TGGCCA 1 cut(s) 328
MluCI AATT 3 cut(s) 40, 191, 211
MluNI TGGCCA 1 cut(s) 328
MlyI GAGTC 1 cut(s) 48
MnlI CCTC 3 cut(s) 25, 152, 283
Mox20I TGGCCA 1 cut(s) 328
MroXI GAANNNNTTC 1 cut(s) 226
MscI TGGCCA 1 cut(s) 328
MseI TTAA 2 cut(s) 164, 177
Msp20I TGGCCA 1 cut(s) 328
MspA1I CMGCKG 1 cut(s) 318
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 1 cut(s) 92
MvaI CCWGG 1 cut(s) 92
MwoI GCNNNNNNNGC 1 cut(s) 187
NdeI CATATG 1 cut(s) 295
NdeII GATC 2 cut(s) 255, 395
NheI GCTAGC 1 cut(s) 8
NlaIII CATG 3 cut(s) 90, 202, 283
NlaIV GGNNCC 1 cut(s) 429
NmuCI GTSAC 1 cut(s) 157
NspI RCATGY 1 cut(s) 90
PagI TCATGA 1 cut(s) 198
PdmI GAANNNNTTC 1 cut(s) 226
PfeI GAWTC 2 cut(s) 69, 355
PkrI GCNGC 2 cut(s) 37, 320
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
PsiI TTATAA 1 cut(s) 216
Psp6I CCWGG 1 cut(s) 90
PspGI CCWGG 1 cut(s) 90
PspN4I GGNNCC 1 cut(s) 429
RsaI GTAC 1 cut(s) 309
RsaNI GTAC 1 cut(s) 308
SaqAI TTAA 2 cut(s) 164, 177
SatI GCNGC 2 cut(s) 36, 319
Sau3AI GATC 2 cut(s) 255, 395
SchI GAGTC 1 cut(s) 48
ScrFI CCNGG 1 cut(s) 92
SetI ASST 3 cut(s) 10, 208, 393
SfaNI GCATC 1 cut(s) 177
Sse9I AATT 3 cut(s) 40, 191, 211
SsiI CCGC 1 cut(s) 318
SspMI CTAG 1 cut(s) 9
StyD4I CCNGG 1 cut(s) 90
TaiI ACGT 2 cut(s) 208, 393
TaqII GACCGA 1 cut(s) 423
TasI AATT 3 cut(s) 40, 191, 211
TauI GCSGC 1 cut(s) 321
TfiI GAWTC 2 cut(s) 69, 355
Tru1I TTAA 2 cut(s) 164, 177
Tru9I TTAA 2 cut(s) 164, 177
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 1 cut(s) 35
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 3 cut(s) 187, 215, 292
TspRI CASTG 1 cut(s) 339
XceI RCATGY 1 cut(s) 90
XmnI GAANNNNTTC 1 cut(s) 226
XspI CTAG 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.