AT3G04460

Required for peroxisome biogenesis and for PTS1- and PTS2-dependent protein import into peroxisomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0009326
Physical Location & Seq
Forward (+)
1186440 .. 1189508
3069 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04460.1

Sequence Viewer

Length: 1182 bp
ATGTTGTTTCAGGTGGGAGGTGAAGGCACACGCCCCACCTTCTTTGAGATGGCTGCTGCTCAGCAACTTCCTGCTAGCCTTCGCGCCGCTCTCACCTATTCCCTCGGCGTTTTTGCTTTGAGACGATCGTTTCTCCATAAGATTTTAGACTATGAGGATGAATTCTTTGCTGCTCTGATGCTTATTCTTGAAGGACACAGCTTACGAACCACAGATGGTTCCTTTGCTGAATCATTGTATGGTTTAAGGAGGAAATCAGCGAGATTGAGATTGAGGAAAGACAGTGCCAGGAAGGATTCTAGCGAAGAAGTTCAACATTCTGGTTTAGAGAAGCGTCAGAGAATCCTTTCTGTTGTGTTTCTGGTTGTGTTACCGTATTTCAAGTCAAAATTGCATGCTATATATAATAAAGAAAGAGAGGCTAGGCTCCGGGAAAGTTTATGGGGTGCAGAGGATCAAGGATTTGATGAAGCTGACTTCTTCACAGGGGATGATTCAATTGTTTCAAGAGAGCCTAGTGGGAATGAAGAGCTTTCTGTTCGAGTACAATTAGCTACCAAAATTAAAAAGTTTATAGCTGTCTGCTATCCTTGGATACATGCATCAAGTGAAGGATTATCGTTCACTTACCAGCTCTTATATCTGCTGGACGCTACTGGATTTTATTCTCTTGGGCTACAAGCTCTTGGGATTCAAGTTTGTAGAGCTACAGGGCAAGAACTGATGGATACATCCTCAAGGATTTCAAAGATTCGGAATCATGAGCGTGAGAGACTTCGTGGTCCTCCATGGTTAAAAACGGTGCAAGGGGCACTTCTTAGCTGCTCATATGCTGTGCTTGACTATGCCCAAACCGGTCTAATTGCAGCAGTTTTTATCTTCAAAATGATGGAATGGTGGTATCAATCCGCTGAAGAGAGATTATCAGCTCCAACAGTGTACCCTCCACCTCCACCTCCTCCAGCCCCAAAGATGGCCAAAGAAGGAATCCCTCTACCTCCAGACAGATCCCTCTGTGCTTTATGCTTGCAAAAACGTGCAAATCCATCAGTTGTCACTGTCTCTGGATTTGTTTTCTGCTATTCTTGCGTATTTAAGTATGTTTCAAAGTACAAGCGATGTCCGGTGACATTGATTCCAGCCAGCGTTGATCAGATTAGGAGGTTGTTTCAGGACACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005779 GO:0006082 GO:0006464 GO:0006513 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009790 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016021 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0030258 GO:0031090 GO:0031224 GO:0031231 GO:0031300 GO:0031301 GO:0031903 GO:0032446 GO:0032501 GO:0032502 GO:0032787 GO:0032991 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042579 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0048598 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901575 GO:1990351 GO:1990415 GO:1990429
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Pex2_Pex12 zf-C3HC4_2

Protein Analysis

393

Amino Acids

44.4

Weight (kDa)

9.06

Isoelectric Point (pI)

50.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex2_Pex12 PF04757 21 - 303 5.8e-43 Pex2 / Pex12 amino terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 780
AccBSI CCGCTC 1 cut(s) 89
AccII CGCG 1 cut(s) 84
AciI CCGC 2 cut(s) 87, 909
AclWI GGATC 2 cut(s) 462, 1002
AcoI YGGCCR 1 cut(s) 975
AcsI RAATTY 1 cut(s) 161
AcuI CTGAAG 1 cut(s) 933
AfaI GTAC 3 cut(s) 546, 941, 1112
AfiI CCNNNNNNNGG 1 cut(s) 973
AgeI ACCGGT 1 cut(s) 854
AgsI TTSAA 9 cut(s) 191, 314, 382, 498, 507, 695, 747, 883, 1107
AjnI CCWGG 1 cut(s) 287
Alw26I GTCTC 3 cut(s) 115, 766, 1066
AlwI GGATC 2 cut(s) 462, 1002
AoxI GGCC 1 cut(s) 975
ApeKI GCWGC 5 cut(s) 53, 56, 170, 822, 866
ApoI RAATTY 1 cut(s) 161
AsiGI ACCGGT 1 cut(s) 854
Asp700I GAANNNNTTC 2 cut(s) 309, 346
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 782
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 3 cut(s) 32, 85, 1138
AsuNHI GCTAGC 1 cut(s) 74
AvaII GGWCC 1 cut(s) 782
BaeGI GKGCMC 1 cut(s) 814
BalI TGGCCA 1 cut(s) 977
BbvI GCAGC 5 cut(s) 40, 43, 157, 809, 878
BccI CCATC 6 cut(s) 43, 209, 718, 883, 967, 1054
BciT130I CCWGG 1 cut(s) 289
BciVI GTATCC 2 cut(s) 588, 721
BclI TGATCA 1 cut(s) 1150
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 3 cut(s) 115, 766, 1066
BfaI CTAG 4 cut(s) 75, 300, 423, 516
BfmI CTRYAG 1 cut(s) 708
BfuI GTATCC 2 cut(s) 588, 721
BisI GCNGC 6 cut(s) 54, 57, 87, 171, 823, 867
BlpI GCTNAGC 1 cut(s) 60
BlsI GCNGC 6 cut(s) 55, 58, 88, 172, 824, 868
Bme1390I CCNGG 2 cut(s) 289, 431
Bme18I GGWCC 1 cut(s) 782
BmgT120I GGNCC 1 cut(s) 782
BmiI GGNNCC 2 cut(s) 220, 428
BmrFI CCNGG 2 cut(s) 289, 431
BmsI GCATC 2 cut(s) 168, 611
BmtI GCTAGC 1 cut(s) 78
BpmI CTGGAG 2 cut(s) 945, 984
Bpu1102I GCTNAGC 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 721
BpuMI CCSGG 1 cut(s) 431
BsaJI CCNNGG 3 cut(s) 103, 590, 788
BsaWI WCCGGW 2 cut(s) 854, 1123
Bsc4I CCNNNNNNNGG 1 cut(s) 973
Bse118I RCCGGY 1 cut(s) 854
Bse1I ACTGG 1 cut(s) 661
BseBI CCWGG 1 cut(s) 289
BseDI CCNNGG 3 cut(s) 103, 590, 788
BseGI GGATG 3 cut(s) 163, 496, 731
BseLI CCNNNNNNNGG 1 cut(s) 973
BseMII CTCAG 1 cut(s) 74
BseNI ACTGG 1 cut(s) 661
BseRI GAGGAG 1 cut(s) 948
BseSI GKGCMC 1 cut(s) 814
BseXI GCAGC 5 cut(s) 40, 43, 157, 809, 878
BsgI GTGCAG 1 cut(s) 468
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 1 cut(s) 977
BshTI ACCGGT 1 cut(s) 854
BsiEI CGRYCG 1 cut(s) 128
BsiSI CCGG 3 cut(s) 430, 855, 1124
BslI CCNNNNNNNGG 1 cut(s) 973
BsmAI GTCTC 3 cut(s) 115, 766, 1066
BsmBI CGTCTC 1 cut(s) 115
BsnI GGCC 1 cut(s) 977
Bsp1286I GDGCHC 1 cut(s) 814
Bsp143I GATC 4 cut(s) 125, 454, 1007, 1150
Bsp1720I GCTNAGC 1 cut(s) 60
Bsp19I CCATGG 1 cut(s) 788
BspACI CCGC 2 cut(s) 87, 909
BspANI GGCC 1 cut(s) 977
BspCNI CTCAG 1 cut(s) 73
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 760
BspLI GGNNCC 2 cut(s) 220, 428
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 2 cut(s) 462, 1002
BspQI GCTCTTC 1 cut(s) 522
BsrBI CCGCTC 1 cut(s) 89
BsrFI RCCGGY 1 cut(s) 854
BsrI ACTGG 1 cut(s) 661
BssAI RCCGGY 1 cut(s) 854
BssECI CCNNGG 3 cut(s) 103, 590, 788
BssMI GATC 4 cut(s) 125, 454, 1007, 1150
BssT1I CCWWGG 2 cut(s) 590, 788
Bst2UI CCWGG 1 cut(s) 289
Bst4CI ACNGT 5 cut(s) 284, 375, 802, 937, 1060
Bst6I CTCTTC 2 cut(s) 522, 909
BstC8I GCNNGC 4 cut(s) 76, 396, 1028, 1144
BstDEI CTNAG 3 cut(s) 60, 818, 1179
BstDSI CCRYGG 1 cut(s) 788
BstF5I GGATG 3 cut(s) 163, 496, 731
BstFNI CGCG 1 cut(s) 84
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 4 cut(s) 128, 457, 1010, 1153
BstMAI GTCTC 3 cut(s) 115, 766, 1066
BstMBI GATC 4 cut(s) 125, 454, 1007, 1150
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 1 cut(s) 1086
BstNI CCWGG 1 cut(s) 289
BstNSI RCATGY 2 cut(s) 398, 602
BstSCI CCNGG 2 cut(s) 287, 429
BstSFI CTRYAG 1 cut(s) 708
BstSLI GKGCMC 1 cut(s) 814
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 40, 43, 157, 809, 878
BstX2I RGATCY 1 cut(s) 1007
BstYI RGATCY 1 cut(s) 1007
BsuI GTATCC 2 cut(s) 588, 721
BsuRI GGCC 1 cut(s) 977
BtgI CCRYGG 1 cut(s) 788
BtgZI GCGATG 1 cut(s) 1132
BtsCI GGATG 3 cut(s) 163, 496, 731
BtsIMutI CAGTG 3 cut(s) 289, 942, 1056
Cac8I GCNNGC 4 cut(s) 76, 396, 1028, 1144
CciI TCATGA 1 cut(s) 760
CfoI GCGC 1 cut(s) 86
Cfr10I RCCGGY 1 cut(s) 854
Cfr13I GGNCC 1 cut(s) 782
CseI GACGC 2 cut(s) 323, 659
Csp6I GTAC 3 cut(s) 545, 940, 1111
CspAI ACCGGT 1 cut(s) 854
CspCI CAANNNNNGTGG 2 cut(s) 25, 60
CviAII CATG 4 cut(s) 395, 599, 761, 789
CviQI GTAC 3 cut(s) 545, 940, 1111
DdeI CTNAG 3 cut(s) 60, 818, 1179
DpnI GATC 4 cut(s) 127, 456, 1009, 1152
DpnII GATC 4 cut(s) 125, 454, 1007, 1150
DrdI GACNNNNNNGTC 1 cut(s) 780
DseDI GACNNNNNNGTC 1 cut(s) 780
EaeI YGGCCR 1 cut(s) 975
Eam1104I CTCTTC 2 cut(s) 522, 909
EarI CTCTTC 2 cut(s) 522, 909
Eco130I CCWWGG 2 cut(s) 590, 788
Eco47I GGWCC 1 cut(s) 782
Eco57I CTGAAG 1 cut(s) 933
EcoRI GAATTC 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 287
EcoT14I CCWWGG 2 cut(s) 590, 788
EcoT22I ATGCAT 1 cut(s) 604
ErhI CCWWGG 2 cut(s) 590, 788
Esp3I CGTCTC 1 cut(s) 115
FaeI CATG 4 cut(s) 398, 602, 764, 792
FalI AAGNNNNNCTT 2 cut(s) 798, 830
FatI CATG 4 cut(s) 394, 598, 760, 788
FauNDI CATATG 1 cut(s) 829
FbaI TGATCA 1 cut(s) 1150
Fnu4HI GCNGC 6 cut(s) 54, 57, 87, 171, 823, 867
FokI GGATG 3 cut(s) 170, 503, 718
Fsp4HI GCNGC 6 cut(s) 54, 57, 87, 171, 823, 867
FspBI CTAG 4 cut(s) 75, 300, 423, 516
GlaI GCGC 1 cut(s) 85
GluI GCNGC 6 cut(s) 54, 57, 87, 171, 823, 867
GsuI CTGGAG 2 cut(s) 945, 984
HaeIII GGCC 1 cut(s) 977
HapII CCGG 3 cut(s) 430, 855, 1124
HgaI GACGC 2 cut(s) 323, 659
HhaI GCGC 1 cut(s) 86
Hin1II CATG 4 cut(s) 398, 602, 764, 792
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 9 cut(s) 230, 296, 342, 494, 691, 751, 757, 987, 1135
HpaII CCGG 3 cut(s) 430, 855, 1124
HphI GGTGA 3 cut(s) 32, 85, 1138
Hpy166II GTNNAC 2 cut(s) 624, 940
Hpy188I TCNGA 4 cut(s) 177, 339, 756, 1155
Hpy188III TCNNGA 6 cut(s) 188, 507, 761, 1001, 1065, 1172
Hpy8I GTNNAC 2 cut(s) 624, 940
HpyAV CCTTC 7 cut(s) 17, 49, 89, 185, 286, 605, 977
HpyCH4III ACNGT 5 cut(s) 284, 375, 802, 937, 1060
HpyCH4IV ACGT 1 cut(s) 1036
HpyCH4V TGCA 7 cut(s) 394, 449, 602, 805, 866, 1030, 1040
HpyF10VI GCNNNNNNNGC 1 cut(s) 1086
HpyF3I CTNAG 3 cut(s) 60, 818, 1179
HpySE526I ACGT 1 cut(s) 1036
Hsp92II CATG 4 cut(s) 398, 602, 764, 792
HspAI GCGC 1 cut(s) 84
Ksp22I TGATCA 1 cut(s) 1150
Kzo9I GATC 4 cut(s) 125, 454, 1007, 1150
LguI GCTCTTC 1 cut(s) 522
LmnI GCTCC 2 cut(s) 432, 934
Lsp1109I GCAGC 5 cut(s) 40, 43, 157, 809, 878
LweI GCATC 2 cut(s) 168, 611
MaeI CTAG 4 cut(s) 75, 300, 423, 516
MaeII ACGT 1 cut(s) 1036
MaeIII GTNAC 3 cut(s) 369, 1054, 1126
MalI GATC 4 cut(s) 127, 456, 1009, 1152
MbiI CCGCTC 1 cut(s) 89
MboI GATC 4 cut(s) 125, 454, 1007, 1150
MboII GAAGA 5 cut(s) 317, 472, 539, 871, 926
MfeI CAATTG 1 cut(s) 498
MflI RGATCY 1 cut(s) 1007
MhlI GDGCHC 1 cut(s) 814
MlsI TGGCCA 1 cut(s) 977
MluCI AATT 6 cut(s) 161, 389, 498, 548, 561, 861
MluNI TGGCCA 1 cut(s) 977
MmeI TCCRAC 1 cut(s) 956
Mox20I TGGCCA 1 cut(s) 977
Mph1103I ATGCAT 1 cut(s) 604
MroXI GAANNNNTTC 2 cut(s) 309, 346
MscI TGGCCA 1 cut(s) 977
MseI TTAA 4 cut(s) 245, 564, 794, 1095
MslI CAYNNNNRTG 1 cut(s) 765
Msp20I TGGCCA 1 cut(s) 977
MspA1I CMGCKG 1 cut(s) 911
MspI CCGG 3 cut(s) 430, 855, 1124
MspR9I CCNGG 2 cut(s) 289, 431
MunI CAATTG 1 cut(s) 498
MvaI CCWGG 1 cut(s) 289
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 1086
NciI CCSGG 1 cut(s) 431
NcoI CCATGG 1 cut(s) 788
NdeI CATATG 1 cut(s) 829
NdeII GATC 4 cut(s) 125, 454, 1007, 1150
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 4 cut(s) 398, 602, 764, 792
NlaIV GGNNCC 2 cut(s) 220, 428
NmeAIII GCCGAG 1 cut(s) 84
NmuCI GTSAC 2 cut(s) 1054, 1126
NsiI ATGCAT 1 cut(s) 604
NspI RCATGY 2 cut(s) 398, 602
PaeI GCATGC 1 cut(s) 398
PagI TCATGA 1 cut(s) 760
PciSI GCTCTTC 1 cut(s) 522
PdmI GAANNNNTTC 2 cut(s) 309, 346
PfeI GAWTC 9 cut(s) 230, 296, 342, 494, 691, 751, 757, 987, 1135
PfoI TCCNGGA 1 cut(s) 429
PinAI ACCGGT 1 cut(s) 854
PkrI GCNGC 6 cut(s) 55, 58, 88, 172, 824, 868
Ple19I CGATCG 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 287
PspGI CCWGG 1 cut(s) 287
PspN4I GGNNCC 2 cut(s) 220, 428
PspPI GGNCC 1 cut(s) 782
PsuI RGATCY 1 cut(s) 1007
PvuI CGATCG 1 cut(s) 128
RsaI GTAC 3 cut(s) 546, 941, 1112
RsaNI GTAC 3 cut(s) 545, 940, 1111
RseI CAYNNNNRTG 1 cut(s) 765
SapI GCTCTTC 1 cut(s) 522
SaqAI TTAA 4 cut(s) 245, 564, 794, 1095
SatI GCNGC 6 cut(s) 54, 57, 87, 171, 823, 867
Sau3AI GATC 4 cut(s) 125, 454, 1007, 1150
Sau96I GGNCC 1 cut(s) 782
ScrFI CCNGG 2 cut(s) 289, 431
SduI GDGCHC 1 cut(s) 814
SfaNI GCATC 2 cut(s) 168, 611
SfcI CTRYAG 1 cut(s) 708
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 1 cut(s) 765
SmlI CTYRAG 1 cut(s) 736
SmoI CTYRAG 1 cut(s) 736
SphI GCATGC 1 cut(s) 398
Sse9I AATT 6 cut(s) 161, 389, 498, 548, 561, 861
SsiI CCGC 2 cut(s) 87, 909
SspMI CTAG 4 cut(s) 75, 300, 423, 516
StyD4I CCNGG 2 cut(s) 287, 429
StyI CCWWGG 2 cut(s) 590, 788
TaaI ACNGT 5 cut(s) 284, 375, 802, 937, 1060
TaiI ACGT 1 cut(s) 1039
TaqI TCGA 1 cut(s) 541
TasI AATT 6 cut(s) 161, 389, 498, 548, 561, 861
TatI WGTACW 2 cut(s) 544, 1110
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 9 cut(s) 230, 296, 342, 494, 691, 751, 757, 987, 1135
Tru1I TTAA 4 cut(s) 245, 564, 794, 1095
Tru9I TTAA 4 cut(s) 245, 564, 794, 1095
TscAI CASTG 3 cut(s) 289, 942, 1063
TseFI GTSAC 2 cut(s) 1054, 1126
TseI GCWGC 5 cut(s) 53, 56, 170, 822, 866
Tsp45I GTSAC 2 cut(s) 1054, 1126
TspDTI ATGAA 3 cut(s) 174, 483, 540
TspRI CASTG 3 cut(s) 289, 942, 1063
VpaK11BI GGWCC 1 cut(s) 782
XapI RAATTY 1 cut(s) 161
XceI RCATGY 2 cut(s) 398, 602
XmnI GAANNNNTTC 2 cut(s) 309, 346
XspI CTAG 4 cut(s) 75, 300, 423, 516
Zsp2I ATGCAT 1 cut(s) 604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.