FvH4_6g01740

Required for peroxisome biogenesis and for PTS1- and PTS2-dependent protein import into peroxisomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009326
Physical Location & Seq
Forward (+)
986836 .. 991882
5047 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g01740.t1

Sequence Viewer

Length: 1182 bp
ATGCTTTTCCAGGTGGGAGGCCAAGGGACTCGTCCCACCTTCTTCGAGATGGCCGCAGCCCAGCAGCTCCCCGCCAGCCTCCGCGCCGCCCTCACCTACTCCGTCGGCGTTTTGGCTTTGCGGAGGCCATTTCTGCACAGGGTTCTTGACTACGAAGACGAGTTCTTCGCTTTCCTCATGCTCGTTCTCGAAACCCATAGCTTAAGGACCACAGATGCTTCGTTTTCGGAATCTTTGTATGGGCTGAGGAGGAGGGCTGTAAAGGTTAAGGTGCAGAAGGAGGATGCGAGGTTGAGCCCCGGTGAGGAATTTCATCATTCCGGGTTGGAGAAGAGGCAGAAAGTACTCTCGGTTGTGTTTCTGGTTGTATTGCCATACTTCAAATCAAAATTGCACGCAATCTATAATAGAGAAAGGGAAGCCAGACTACAAGCAACTTTATGGGGAGATAGTGGTGAAAGATTTGATGATGCTGATATAGTTGATAGAGGCGACGGTTCTGGTGTTTCAAGAGGAAGTTTCGATGCAGAAGCATCAGTTAGGACACGTTTGGCAAAGAAGATCCAGAAACTTATAGGTGCTTGCTACCCATGGGTGCATGCTAGCAGTGAAGGATTTTCATTTGCTTACCAGATGTTATACTTGTTGGATGCGACGGGATTTTATTCTTTAGGATTGCATGTGCTTGGGATTCAAGTCTGTCGAGCTACTGGGCAAGAGCTGATGGATACATCTTCTAGAATTTCAAAAACAAGAAGCCGTGAACGTGAGAGACTCCGTGGTCCGCCATGGTTGAAGGCAGTACAAGGAGCATTGCTCAGTGGTACGTACACAGTCCTGGATTATGCGCAGACTGGTTTAATTGCAGCAGTATTCTTCTTTAAAATGATGGAATGGTGGTACCAATCTGCTGAAGAGAGAATGTCAGCTCCAACCATATACCCCCCACCTCCACCACCTCCTTCTCCCCAGGTTGCCAAAGAGGGGATTCCATTGCCTTCCGATAGAACTCTTTGTCCTCTCTGTTCACAGAAACGCGCAAATCCATCTGTCATTACAGTTTCTGGGTTTGTCTTCTGCTATGCCTGCGTTTTCAAGTATGTTACTCAGTATAAGCGTTGTCCAGTCACATTGATGCCTGCTAACGTTGACCAGATAAGAAGGCTCTTTCATGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005779 GO:0006082 GO:0006464 GO:0006513 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009790 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016021 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0030258 GO:0031090 GO:0031224 GO:0031231 GO:0031300 GO:0031301 GO:0031903 GO:0032446 GO:0032501 GO:0032502 GO:0032787 GO:0032991 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042579 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0048598 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901575 GO:1990351 GO:1990415 GO:1990429
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Pex2_Pex12 zf-C3HC4_2

Protein Analysis

394

Amino Acids

44.31

Weight (kDa)

9.29

Isoelectric Point (pI)

45.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex2_Pex12 PF04757 21 - 303 2.9e-42 Pex2 / Pex12 amino terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 780
Acc16I TGCGCA 1 cut(s) 849
Acc65I GGTACC 1 cut(s) 900
AccB1I GGYRCC 1 cut(s) 900
AccII CGCG 2 cut(s) 84, 1038
AciI CCGC 6 cut(s) 54, 72, 82, 87, 121, 785
AclI AACGTT 1 cut(s) 1146
AclWI GGATC 1 cut(s) 556
AcoI YGGCCR 1 cut(s) 51
AcsI RAATTY 2 cut(s) 308, 741
AcuI CTGAAG 1 cut(s) 933
AfaI GTAC 5 cut(s) 345, 804, 826, 830, 902
AfiI CCNNNNNNNGG 2 cut(s) 304, 984
AflII CTTAAG 1 cut(s) 202
AflIII ACRYGT 1 cut(s) 545
AgsI TTSAA 6 cut(s) 382, 510, 695, 747, 796, 1096
AjnI CCWGG 3 cut(s) 9, 837, 969
AloI GAACNNNNNNTCC 2 cut(s) 1000, 1032
AluBI AGCT 5 cut(s) 67, 201, 707, 721, 929
AluI AGCT 5 cut(s) 67, 201, 707, 721, 929
Alw26I GTCTC 1 cut(s) 766
AlwI GGATC 1 cut(s) 556
AlwNI CAGNNNCTG 1 cut(s) 1064
AoxI GGCC 3 cut(s) 19, 51, 125
ApeKI GCWGC 3 cut(s) 56, 64, 866
ApoI RAATTY 2 cut(s) 308, 741
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
Asp718I GGTACC 1 cut(s) 900
AspLEI GCGC 3 cut(s) 86, 850, 1040
AspS9I GGNCC 2 cut(s) 207, 782
AsuC2I CCSGG 2 cut(s) 300, 322
AsuHPI GGTGA 3 cut(s) 85, 314, 467
AsuNHI GCTAGC 1 cut(s) 602
AvaII GGWCC 2 cut(s) 207, 782
BanI GGYRCC 1 cut(s) 900
BanII GRGCYC 1 cut(s) 299
BarI GAAGNNNNNNTAC 2 cut(s) 411, 443
BbsI GAAGAC 2 cut(s) 162, 1066
BbvCI CCTCAGC 1 cut(s) 245
BbvI GCAGC 3 cut(s) 68, 76, 878
BccI CCATC 4 cut(s) 43, 718, 883, 1054
BceAI ACGGC 1 cut(s) 744
BciT130I CCWGG 3 cut(s) 11, 839, 971
BciVI GTATCC 1 cut(s) 721
BcnI CCSGG 2 cut(s) 300, 322
BcoDI GTCTC 1 cut(s) 766
BfaI CTAG 2 cut(s) 603, 738
BfrI CTTAAG 1 cut(s) 202
BfuI GTATCC 1 cut(s) 721
BisI GCNGC 5 cut(s) 54, 57, 65, 87, 867
BlsI GCNGC 5 cut(s) 55, 58, 66, 88, 868
BmcAI AGTACT 1 cut(s) 345
Bme1390I CCNGG 5 cut(s) 11, 300, 322, 839, 971
Bme18I GGWCC 2 cut(s) 207, 782
BmgT120I GGNCC 2 cut(s) 207, 782
BmiI GGNNCC 1 cut(s) 902
BmrFI CCNGG 5 cut(s) 11, 300, 322, 839, 971
BmrI ACTGGG 1 cut(s) 720
BmsI GCATC 7 cut(s) 205, 274, 460, 514, 542, 640, 1125
BmtI GCTAGC 1 cut(s) 606
BmuI ACTGGG 1 cut(s) 720
BpiI GAAGAC 2 cut(s) 162, 1066
BplI GAGNNNNNCTC 2 cut(s) 801, 833
Bpu10I CCTNAGC 1 cut(s) 245
BpuMI CCSGG 2 cut(s) 300, 322
BsaAI YACGTR 1 cut(s) 828
BsaJI CCNNGG 6 cut(s) 22, 298, 590, 778, 788, 969
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 984
Bse1I ACTGG 3 cut(s) 715, 859, 1124
Bse3DI GCAATG 2 cut(s) 812, 992
BseBI CCWGG 3 cut(s) 11, 839, 971
BseDI CCNNGG 6 cut(s) 22, 298, 590, 778, 788, 969
BseGI GGATG 2 cut(s) 289, 655
BseLI CCNNNNNNNGG 2 cut(s) 304, 984
BseMI GCAATG 2 cut(s) 812, 992
BseMII CTCAG 3 cut(s) 236, 832, 1121
BseNI ACTGG 3 cut(s) 715, 859, 1124
BseRI GAGGAG 2 cut(s) 262, 265
BseXI GCAGC 3 cut(s) 68, 76, 878
BseYI CCCAGC 1 cut(s) 60
BsgI GTGCAG 2 cut(s) 119, 293
Bsh1236I CGCG 2 cut(s) 84, 1038
BshFI GGCC 3 cut(s) 21, 53, 127
BshNI GGYRCC 1 cut(s) 900
BsiSI CCGG 2 cut(s) 300, 321
BslFI GGGAC 2 cut(s) 18, 40
BslI CCNNNNNNNGG 2 cut(s) 304, 984
BsmAI GTCTC 1 cut(s) 766
BsmFI GGGAC 2 cut(s) 18, 40
BsnI GGCC 3 cut(s) 21, 53, 127
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 1 cut(s) 561
Bsp19I CCATGG 2 cut(s) 590, 788
BspACI CCGC 6 cut(s) 54, 72, 82, 87, 121, 785
BspANI GGCC 3 cut(s) 21, 53, 127
BspCNI CTCAG 3 cut(s) 237, 831, 1120
BspFNI CGCG 2 cut(s) 84, 1038
BspHI TCATGA 1 cut(s) 1171
BspLI GGNNCC 1 cut(s) 902
BspOI GCTAGC 1 cut(s) 606
BspPI GGATC 1 cut(s) 556
BspT107I GGYRCC 1 cut(s) 900
BspTI CTTAAG 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 812, 992
BsrI ACTGG 3 cut(s) 715, 859, 1124
BssECI CCNNGG 6 cut(s) 22, 298, 590, 778, 788, 969
BssMI GATC 1 cut(s) 561
BssT1I CCWWGG 3 cut(s) 22, 590, 788
Bst2UI CCWGG 3 cut(s) 11, 839, 971
Bst4CI ACNGT 3 cut(s) 497, 835, 1060
Bst6I CTCTTC 2 cut(s) 326, 909
BstAFI CTTAAG 1 cut(s) 202
BstBAI YACGTR 1 cut(s) 828
BstC8I GCNNGC 7 cut(s) 76, 396, 583, 600, 604, 1087, 1140
BstDEI CTNAG 3 cut(s) 245, 818, 1107
BstDSI CCRYGG 3 cut(s) 590, 778, 788
BstF5I GGATG 2 cut(s) 289, 655
BstFNI CGCG 2 cut(s) 84, 1038
BstHHI GCGC 3 cut(s) 86, 850, 1040
BstKTI GATC 1 cut(s) 564
BstMAI GTCTC 1 cut(s) 766
BstMBI GATC 1 cut(s) 561
BstMWI GCNNNNNNNGC 2 cut(s) 133, 1086
BstNI CCWGG 3 cut(s) 11, 839, 971
BstNSI RCATGY 2 cut(s) 602, 683
BstSCI CCNGG 5 cut(s) 9, 298, 320, 837, 969
BstSNI TACGTA 1 cut(s) 828
BstUI CGCG 2 cut(s) 84, 1038
BstV1I GCAGC 3 cut(s) 68, 76, 878
BstV2I GAAGAC 2 cut(s) 162, 1066
BstX2I RGATCY 1 cut(s) 561
BstYI RGATCY 1 cut(s) 561
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 3 cut(s) 21, 53, 127
BtgI CCRYGG 3 cut(s) 590, 778, 788
BtsCI GGATG 2 cut(s) 289, 655
BtsI GCAGTG 1 cut(s) 613
BtsIMutI CAGTG 2 cut(s) 613, 826
Cac8I GCNNGC 7 cut(s) 76, 396, 583, 600, 604, 1087, 1140
CaiI CAGNNNCTG 1 cut(s) 1064
CciI TCATGA 1 cut(s) 1171
CfoI GCGC 3 cut(s) 86, 850, 1040
Cfr13I GGNCC 2 cut(s) 207, 782
Csp6I GTAC 5 cut(s) 344, 803, 825, 829, 901
CviAII CATG 6 cut(s) 178, 591, 599, 680, 789, 1172
CviQI GTAC 5 cut(s) 344, 803, 825, 829, 901
DdeI CTNAG 3 cut(s) 245, 818, 1107
DpnI GATC 1 cut(s) 563
DpnII GATC 1 cut(s) 561
DraI TTTAAA 1 cut(s) 883
DrdI GACNNNNNNGTC 1 cut(s) 780
DseDI GACNNNNNNGTC 1 cut(s) 780
EaeI YGGCCR 1 cut(s) 51
Eam1104I CTCTTC 2 cut(s) 326, 909
EarI CTCTTC 2 cut(s) 326, 909
EciI GGCGGA 1 cut(s) 774
Eco105I TACGTA 1 cut(s) 828
Eco130I CCWWGG 3 cut(s) 22, 590, 788
Eco24I GRGCYC 1 cut(s) 299
Eco47I GGWCC 2 cut(s) 207, 782
Eco57I CTGAAG 1 cut(s) 933
EcoRII CCWGG 3 cut(s) 9, 837, 969
EcoT14I CCWWGG 3 cut(s) 22, 590, 788
EcoT38I GRGCYC 1 cut(s) 299
ErhI CCWWGG 3 cut(s) 22, 590, 788
FaeI CATG 6 cut(s) 181, 594, 602, 683, 792, 1175
FaqI GGGAC 2 cut(s) 18, 40
FatI CATG 6 cut(s) 177, 590, 598, 679, 788, 1171
FauI CCCGC 1 cut(s) 79
Fnu4HI GCNGC 5 cut(s) 54, 57, 65, 87, 867
FokI GGATG 2 cut(s) 296, 662
FriOI GRGCYC 1 cut(s) 299
Fsp4HI GCNGC 5 cut(s) 54, 57, 65, 87, 867
FspBI CTAG 2 cut(s) 603, 738
FspI TGCGCA 1 cut(s) 849
GlaI GCGC 3 cut(s) 85, 849, 1039
GluI GCNGC 5 cut(s) 54, 57, 65, 87, 867
GsaI CCCAGC 1 cut(s) 64
HaeIII GGCC 3 cut(s) 21, 53, 127
HapII CCGG 2 cut(s) 300, 321
HhaI GCGC 3 cut(s) 86, 850, 1040
Hin1II CATG 6 cut(s) 181, 594, 602, 683, 792, 1175
Hin6I GCGC 3 cut(s) 84, 848, 1038
HinP1I GCGC 3 cut(s) 84, 848, 1038
HincII GTYRAC 1 cut(s) 1150
HindII GTYRAC 1 cut(s) 1150
HinfI GANTC 5 cut(s) 28, 230, 691, 774, 988
HpaII CCGG 2 cut(s) 300, 321
HphI GGTGA 3 cut(s) 85, 314, 467
Hpy166II GTNNAC 4 cut(s) 764, 831, 1028, 1150
Hpy188I TCNGA 2 cut(s) 229, 1003
Hpy188III TCNNGA 7 cut(s) 46, 146, 188, 510, 565, 738, 1172
Hpy8I GTNNAC 4 cut(s) 764, 831, 1028, 1150
Hpy99I CGWCG 3 cut(s) 107, 497, 658
HpyAV CCTTC 7 cut(s) 49, 271, 605, 790, 972, 1008, 1155
HpyCH4III ACNGT 3 cut(s) 497, 835, 1060
HpyCH4IV ACGT 4 cut(s) 547, 766, 827, 1146
HpyCH4V TGCA 7 cut(s) 136, 274, 394, 527, 598, 679, 866
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 1086
HpyF3I CTNAG 3 cut(s) 245, 818, 1107
HpySE526I ACGT 4 cut(s) 547, 766, 827, 1146
Hsp92II CATG 6 cut(s) 181, 594, 602, 683, 792, 1175
HspAI GCGC 3 cut(s) 84, 848, 1038
KpnI GGTACC 1 cut(s) 904
Kzo9I GATC 1 cut(s) 561
LmnI GCTCC 3 cut(s) 72, 809, 934
Lsp1109I GCAGC 3 cut(s) 68, 76, 878
LweI GCATC 7 cut(s) 205, 274, 460, 514, 542, 640, 1125
MaeI CTAG 2 cut(s) 603, 738
MaeII ACGT 4 cut(s) 547, 766, 827, 1146
MaeIII GTNAC 2 cut(s) 1102, 1126
MalI GATC 1 cut(s) 563
MboI GATC 1 cut(s) 561
MboII GAAGA 9 cut(s) 34, 157, 167, 343, 571, 726, 868, 926, 1066
MflI RGATCY 1 cut(s) 561
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 4 cut(s) 308, 389, 741, 861
MlyI GAGTC 2 cut(s) 22, 768
MmeI TCCRAC 3 cut(s) 306, 627, 956
MseI TTAA 4 cut(s) 203, 267, 860, 882
MslI CAYNNNNRTG 1 cut(s) 1133
MspCI CTTAAG 1 cut(s) 202
MspI CCGG 2 cut(s) 300, 321
MspR9I CCNGG 5 cut(s) 11, 300, 322, 839, 971
MvaI CCWGG 3 cut(s) 11, 839, 971
MvnI CGCG 2 cut(s) 84, 1038
MwoI GCNNNNNNNGC 2 cut(s) 133, 1086
NciI CCSGG 2 cut(s) 300, 322
NcoI CCATGG 2 cut(s) 590, 788
NdeII GATC 1 cut(s) 561
NheI GCTAGC 1 cut(s) 602
NlaIII CATG 6 cut(s) 181, 594, 602, 683, 792, 1175
NlaIV GGNNCC 1 cut(s) 902
NmuCI GTSAC 1 cut(s) 1126
NsbI TGCGCA 1 cut(s) 849
NspI RCATGY 2 cut(s) 602, 683
PaeI GCATGC 1 cut(s) 602
PagI TCATGA 1 cut(s) 1171
PfeI GAWTC 3 cut(s) 230, 691, 988
PfoI TCCNGGA 1 cut(s) 837
PkrI GCNGC 5 cut(s) 55, 58, 66, 88, 868
PleI GAGTC 2 cut(s) 22, 768
PpsI GAGTC 2 cut(s) 22, 768
Ppu21I YACGTR 1 cut(s) 828
Psp1406I AACGTT 1 cut(s) 1146
Psp6I CCWGG 3 cut(s) 9, 837, 969
PspFI CCCAGC 1 cut(s) 60
PspGI CCWGG 3 cut(s) 9, 837, 969
PspN4I GGNNCC 1 cut(s) 902
PspPI GGNCC 2 cut(s) 207, 782
PstNI CAGNNNCTG 1 cut(s) 1064
PsuI RGATCY 1 cut(s) 561
RsaI GTAC 5 cut(s) 345, 804, 826, 830, 902
RsaNI GTAC 5 cut(s) 344, 803, 825, 829, 901
RseI CAYNNNNRTG 1 cut(s) 1133
SaqAI TTAA 4 cut(s) 203, 267, 860, 882
SatI GCNGC 5 cut(s) 54, 57, 65, 87, 867
Sau3AI GATC 1 cut(s) 561
Sau96I GGNCC 2 cut(s) 207, 782
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 2 cut(s) 22, 768
ScrFI CCNGG 5 cut(s) 11, 300, 322, 839, 971
SduI GDGCHC 1 cut(s) 299
SfaNI GCATC 7 cut(s) 205, 274, 460, 514, 542, 640, 1125
SinI GGWCC 2 cut(s) 207, 782
SmiMI CAYNNNNRTG 1 cut(s) 1133
SmlI CTYRAG 1 cut(s) 202
SmoI CTYRAG 1 cut(s) 202
SnaBI TACGTA 1 cut(s) 828
SphI GCATGC 1 cut(s) 602
Sse9I AATT 4 cut(s) 308, 389, 741, 861
SsiI CCGC 6 cut(s) 54, 72, 82, 87, 121, 785
SspMI CTAG 2 cut(s) 603, 738
StyD4I CCNGG 5 cut(s) 9, 298, 320, 837, 969
StyI CCWWGG 3 cut(s) 22, 590, 788
TaaI ACNGT 3 cut(s) 497, 835, 1060
TaiI ACGT 4 cut(s) 550, 769, 830, 1149
TaqI TCGA 4 cut(s) 45, 189, 522, 703
TasI AATT 4 cut(s) 308, 389, 741, 861
TatI WGTACW 2 cut(s) 343, 802
TauI GCSGC 2 cut(s) 56, 89
TfiI GAWTC 3 cut(s) 230, 691, 988
Tru1I TTAA 4 cut(s) 203, 267, 860, 882
Tru9I TTAA 4 cut(s) 203, 267, 860, 882
TscAI CASTG 2 cut(s) 613, 826
TseFI GTSAC 1 cut(s) 1126
TseI GCWGC 3 cut(s) 56, 64, 866
Tsp45I GTSAC 1 cut(s) 1126
TspDTI ATGAA 3 cut(s) 302, 609, 1160
TspGWI ACGGA 2 cut(s) 91, 767
TspRI CASTG 2 cut(s) 613, 826
Vha464I CTTAAG 1 cut(s) 202
VpaK11BI GGWCC 2 cut(s) 207, 782
XapI RAATTY 2 cut(s) 308, 741
XbaI TCTAGA 1 cut(s) 737
XceI RCATGY 2 cut(s) 602, 683
XspI CTAG 2 cut(s) 603, 738
ZrmI AGTACT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.