MD00G1148800.v1.1

Required for peroxisome biogenesis and for PTS1- and PTS2-dependent protein import into peroxisomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009326
Physical Location & Seq
Reverse (-)
32797441 .. 32799835
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1148800.v1.1.491

Sequence Viewer

Length: 579 bp
ATGCTCATTCTCGAAACTCATAGCTTACGAACTACAGATGCTTCGTTTTCCGAATTACTATACGGGTTGAGAAGGAGGGCTGTGAAGATAAAAGCTATGAGGAATGATGATGCTGGACTGAACCCTGGTGATGGAATTCAGCATTATGGCTTAGAGAAGCGCCAGAGATTCTTTCAGTTGTGTTTTTGTACTGGAGCAATTGGTGATTGTTGGGACTTGTTGCCTTACTTTAAGTCAAAATTGCATTCAATCTACAATAGAGAAAGGGAAGCGAGACTTCAAGCAAGTTTATGGGGACACGTTGATGAAAGATTTGATGATATTGACACATTTGGTAGAGGAGAGGATGCTGTTGTTTCGCGAGGAACTTCGGATATGGTAGTATCAGTTAGGACCCGTTTGACAAAAAAAATCCAGAAATTTATAGGTGCTTGGTACCCGTTGCTGCACGCTAATCGTGAAGGATTACATGCGCTTGGGATTCATGTCTGTCGAGCTGCAGGCCAAGAGCTGGTATATAGTATGGATACTTCTTCTAGAAAACAAAACGTGAGAGACATTGTGGCCCTCCATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005779 GO:0006082 GO:0006464 GO:0006513 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009790 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016021 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0030258 GO:0031090 GO:0031224 GO:0031231 GO:0031300 GO:0031301 GO:0031903 GO:0032446 GO:0032501 GO:0032502 GO:0032787 GO:0032991 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042579 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0048598 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901575 GO:1990351 GO:1990415 GO:1990429
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Pex2_Pex12 zf-C3HC4_2

Protein Analysis

193

Amino Acids

21.88

Weight (kDa)

9.11

Isoelectric Point (pI)

28.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex2_Pex12 PF04757 2 - 155 6.4e-08 Pex2 / Pex12 amino terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 435
AccB1I GGYRCC 1 cut(s) 435
AccB7I CCANNNNNTGG 1 cut(s) 511
AccII CGCG 1 cut(s) 361
AcsI RAATTY 2 cut(s) 135, 419
AfaI GTAC 2 cut(s) 190, 437
AfiI CCNNNNNNNGG 2 cut(s) 131, 511
AflIII ACRYGT 1 cut(s) 298
AgsI TTSAA 2 cut(s) 249, 281
AjnI CCWGG 1 cut(s) 124
AluBI AGCT 4 cut(s) 24, 95, 497, 511
AluI AGCT 4 cut(s) 24, 95, 497, 511
Alw26I GTCTC 2 cut(s) 268, 549
AoxI GGCC 2 cut(s) 502, 564
ApeKI GCWGC 2 cut(s) 445, 497
ApoI RAATTY 2 cut(s) 135, 419
Asp718I GGTACC 1 cut(s) 435
AspLEI GCGC 2 cut(s) 162, 475
AspS9I GGNCC 2 cut(s) 393, 565
AsuHPI GGTGA 2 cut(s) 140, 215
AvaII GGWCC 1 cut(s) 393
BanI GGYRCC 1 cut(s) 435
BarI GAAGNNNNNNTAC 2 cut(s) 514, 546
BbvI GCAGC 2 cut(s) 432, 484
BccI CCATC 1 cut(s) 125
BcgI CGANNNNNNTGC 2 cut(s) 437, 471
BciT130I CCWGG 1 cut(s) 126
BciVI GTATCC 1 cut(s) 520
BcoDI GTCTC 2 cut(s) 268, 549
BfaI CTAG 1 cut(s) 537
BfmI CTRYAG 2 cut(s) 33, 498
BfoI RGCGCY 1 cut(s) 163
BfuI GTATCC 1 cut(s) 520
BisI GCNGC 2 cut(s) 446, 498
BlsI GCNGC 2 cut(s) 447, 499
Bme1390I CCNGG 1 cut(s) 126
Bme18I GGWCC 1 cut(s) 393
BmgT120I GGNCC 2 cut(s) 393, 565
BmiI GGNNCC 2 cut(s) 395, 437
BmrFI CCNGG 1 cut(s) 126
BmsI GCATC 3 cut(s) 28, 100, 337
BpmI CTGGAG 1 cut(s) 213
BsaJI CCNNGG 2 cut(s) 124, 571
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 511
Bse1I ACTGG 1 cut(s) 196
BseBI CCWGG 1 cut(s) 126
BseDI CCNNGG 2 cut(s) 124, 571
BseGI GGATG 1 cut(s) 352
BseLI CCNNNNNNNGG 2 cut(s) 131, 511
BseNI ACTGG 1 cut(s) 196
BseRI GAGGAG 1 cut(s) 354
BseXI GCAGC 2 cut(s) 432, 484
BsgI GTGCAG 1 cut(s) 431
Bsh1236I CGCG 1 cut(s) 361
BshFI GGCC 2 cut(s) 504, 566
BshNI GGYRCC 1 cut(s) 435
BslFI GGGAC 2 cut(s) 227, 309
BslI CCNNNNNNNGG 2 cut(s) 131, 511
BsmAI GTCTC 2 cut(s) 268, 549
BsmFI GGGAC 2 cut(s) 227, 309
BsmI GAATGC 1 cut(s) 244
BsnI GGCC 2 cut(s) 504, 566
Bsp19I CCATGG 1 cut(s) 571
Bsp68I TCGCGA 1 cut(s) 361
BspANI GGCC 2 cut(s) 504, 566
BspFNI CGCG 1 cut(s) 361
BspLI GGNNCC 2 cut(s) 395, 437
BspMAI CTGCAG 1 cut(s) 502
BspT107I GGYRCC 1 cut(s) 435
BsrI ACTGG 1 cut(s) 196
BssECI CCNNGG 2 cut(s) 124, 571
BssT1I CCWWGG 1 cut(s) 571
Bst2UI CCWGG 1 cut(s) 126
BstC8I GCNNGC 2 cut(s) 450, 502
BstDEI CTNAG 1 cut(s) 151
BstDSI CCRYGG 1 cut(s) 571
BstF5I GGATG 1 cut(s) 352
BstFNI CGCG 1 cut(s) 361
BstH2I RGCGCY 1 cut(s) 163
BstHHI GCGC 2 cut(s) 162, 475
BstMAI GTCTC 2 cut(s) 268, 549
BstNI CCWGG 1 cut(s) 126
BstNSI RCATGY 1 cut(s) 473
BstSCI CCNGG 1 cut(s) 124
BstSFI CTRYAG 2 cut(s) 33, 498
BstUI CGCG 1 cut(s) 361
BstV1I GCAGC 2 cut(s) 432, 484
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 2 cut(s) 504, 566
BtgI CCRYGG 1 cut(s) 571
BtsCI GGATG 1 cut(s) 352
BtuMI TCGCGA 1 cut(s) 361
Cac8I GCNNGC 2 cut(s) 450, 502
CfoI GCGC 2 cut(s) 162, 475
Cfr13I GGNCC 2 cut(s) 393, 565
Csp6I GTAC 2 cut(s) 189, 436
CviAII CATG 3 cut(s) 470, 485, 572
CviJI RGCY 8 cut(s) 24, 80, 95, 150, 497, 504, 511, 566
CviKI_1 RGCY 8 cut(s) 24, 80, 95, 150, 497, 504, 511, 566
CviQI GTAC 2 cut(s) 189, 436
DdeI CTNAG 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 571
Eco47I GGWCC 1 cut(s) 393
EcoO109I RGGNCCY 1 cut(s) 393
EcoRI GAATTC 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 124
EcoT14I CCWWGG 1 cut(s) 571
ErhI CCWWGG 1 cut(s) 571
FaeI CATG 3 cut(s) 473, 488, 575
FalI AAGNNNNNCTT 2 cut(s) 261, 293
FaqI GGGAC 2 cut(s) 227, 309
FatI CATG 3 cut(s) 469, 484, 571
Fnu4HI GCNGC 2 cut(s) 446, 498
FokI GGATG 1 cut(s) 359
Fsp4HI GCNGC 2 cut(s) 446, 498
FspBI CTAG 1 cut(s) 537
GlaI GCGC 2 cut(s) 161, 474
GluI GCNGC 2 cut(s) 446, 498
GsuI CTGGAG 1 cut(s) 213
HaeII RGCGCY 1 cut(s) 163
HaeIII GGCC 2 cut(s) 504, 566
HhaI GCGC 2 cut(s) 162, 475
Hin1II CATG 3 cut(s) 473, 488, 575
Hin6I GCGC 2 cut(s) 160, 473
HinP1I GCGC 2 cut(s) 160, 473
HinfI GANTC 2 cut(s) 168, 481
HphI GGTGA 2 cut(s) 140, 215
Hpy188I TCNGA 2 cut(s) 52, 373
Hpy188III TCNNGA 5 cut(s) 11, 360, 415, 458, 537
HpyAV CCTTC 2 cut(s) 66, 455
HpyCH4IV ACGT 2 cut(s) 300, 549
HpyCH4V TGCA 3 cut(s) 244, 448, 500
HpyF3I CTNAG 1 cut(s) 151
HpySE526I ACGT 2 cut(s) 300, 549
Hsp92II CATG 3 cut(s) 473, 488, 575
HspAI GCGC 2 cut(s) 160, 473
KpnI GGTACC 1 cut(s) 439
LmnI GCTCC 1 cut(s) 194
LpnPI CCDG 8 cut(s) 99, 111, 138, 176, 177, 428, 486, 497
Lsp1109I GCAGC 2 cut(s) 432, 484
LweI GCATC 3 cut(s) 28, 100, 337
MaeI CTAG 1 cut(s) 537
MaeII ACGT 2 cut(s) 300, 549
MboII GAAGA 2 cut(s) 97, 525
MfeI CAATTG 1 cut(s) 198
MluCI AATT 5 cut(s) 53, 135, 198, 239, 419
MnlI CCTC 6 cut(s) 69, 93, 332, 337, 356, 578
MseI TTAA 1 cut(s) 231
MslI CAYNNNNRTG 1 cut(s) 303
MspR9I CCNGG 1 cut(s) 126
MunI CAATTG 1 cut(s) 198
Mva1269I GAATGC 1 cut(s) 244
MvaI CCWGG 1 cut(s) 126
MvnI CGCG 1 cut(s) 361
NcoI CCATGG 1 cut(s) 571
NlaIII CATG 3 cut(s) 473, 488, 575
NlaIV GGNNCC 2 cut(s) 395, 437
NruI TCGCGA 1 cut(s) 361
NspI RCATGY 1 cut(s) 473
PctI GAATGC 1 cut(s) 244
PfeI GAWTC 2 cut(s) 168, 481
PflMI CCANNNNNTGG 1 cut(s) 511
PkrI GCNGC 2 cut(s) 447, 499
PpuMI RGGWCCY 1 cut(s) 393
Psp5II RGGWCCY 1 cut(s) 393
Psp6I CCWGG 1 cut(s) 124
PspGI CCWGG 1 cut(s) 124
PspN4I GGNNCC 2 cut(s) 395, 437
PspPI GGNCC 2 cut(s) 393, 565
PspPPI RGGWCCY 1 cut(s) 393
PstI CTGCAG 1 cut(s) 502
RruI TCGCGA 1 cut(s) 361
RsaI GTAC 2 cut(s) 190, 437
RsaNI GTAC 2 cut(s) 189, 436
RseI CAYNNNNRTG 1 cut(s) 303
SaqAI TTAA 1 cut(s) 231
SatI GCNGC 2 cut(s) 446, 498
Sau96I GGNCC 2 cut(s) 393, 565
ScrFI CCNGG 1 cut(s) 126
SetI ASST 7 cut(s) 26, 97, 303, 430, 499, 513, 552
SfaNI GCATC 3 cut(s) 28, 100, 337
SfcI CTRYAG 2 cut(s) 33, 498
SinI GGWCC 1 cut(s) 393
SmiMI CAYNNNNRTG 1 cut(s) 303
Sse9I AATT 5 cut(s) 53, 135, 198, 239, 419
SspMI CTAG 1 cut(s) 537
StyD4I CCNGG 1 cut(s) 124
StyI CCWWGG 1 cut(s) 571
TaiI ACGT 2 cut(s) 303, 552
TaqI TCGA 2 cut(s) 12, 493
TasI AATT 5 cut(s) 53, 135, 198, 239, 419
TatI WGTACW 1 cut(s) 188
TfiI GAWTC 2 cut(s) 168, 481
Tru1I TTAA 1 cut(s) 231
Tru9I TTAA 1 cut(s) 231
TseI GCWGC 2 cut(s) 445, 497
TspDTI ATGAA 2 cut(s) 321, 473
Van91I CCANNNNNTGG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 393
XapI RAATTY 2 cut(s) 135, 419
XbaI TCTAGA 1 cut(s) 536
XceI RCATGY 1 cut(s) 473
XspI CTAG 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.