Rh3BG019700

Required for peroxisome biogenesis and for PTS1- and PTS2-dependent protein import into peroxisomes

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0009326
Physical Location & Seq
Forward (+)
1238817 .. 1258530
19714 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG019700.1

Sequence Viewer

Length: 915 bp
ATGAAGGTGCAGAAGGACGATTCTCGGGTGAACTTAGGTGATGAATTTCATCATTCTGGGTTGGAAAGGAGACAGAAAGTTCTCTCAGTAGTGTTTTTGGTTGTATTGCCCTACTTCAAATCGAAATTGCACGCAATCTATAATAGGGAAAGGGAAGCAAGACTACAAGCAACTTTATGGGGAGATAGTGGTGAGAGATTTGACGACCCTGACATAATTGATAGGGGCGAGCGTTCTGTTGTTTCAAGAAGTTTGGATGTGGAAGCATCAGTGAGGACACGTTTGGCAAAGAAGATCCAGAAATTGATAGGTGCTTGCTACCCATGGCTGCATGCTAGTAGTGAAGGATTTTCATTTGCTTACCAGATGTTATACTTGTTGGATGCGACGGGATTTTATTCTTTAGGATTACATGCGCTTGGGATTCAAGTTTGTCGAGCTACTGGGCAAGAGCTGATGGATACTTCTTCTAGAATTTCAAAAACAAGAAGCCGTGAACGTGAGAGACTTCGTGGTCCTCCATGGTTGAAGGCAATACAAGGAGCATTGCTCAGTGGTACATACACAGTCCTGGATTATGCGCAGACTGGTTTAATTGCAGCAGTATTCTTTTTTAAAATGATGGAATGGTGGTACCAATCTGCTGAAGAGAGAATGTCAGCTCCAACCATATACCCCCCACCTCCACCGCCTCCTTCCCCCCAGGTTGCCAAAGAGGGGATTCCACTGCCTTCCGATAGAACTCTTTGTCCTCTCTGTTCACAGAAACGCGCGAATCCATCTGTAATTACAGTTTCTGGGTTTGTCTTTTGCTATGCCTGCGTTTTCAAGTATGTTTCTCAGTATAAGCGCTGTCCAGTCACATTGATGCCTGCTAACGTTGACCAGATAAGAAGGCTCTTTCATGATGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005779 GO:0006082 GO:0006464 GO:0006513 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006807 GO:0006810 GO:0006886 GO:0006996 GO:0007031 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009062 GO:0009653 GO:0009790 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016021 GO:0016042 GO:0016043 GO:0016054 GO:0016558 GO:0016567 GO:0016740 GO:0017038 GO:0019395 GO:0019538 GO:0019752 GO:0019787 GO:0030258 GO:0031090 GO:0031224 GO:0031231 GO:0031300 GO:0031301 GO:0031903 GO:0032446 GO:0032501 GO:0032502 GO:0032787 GO:0032991 GO:0033036 GO:0033365 GO:0034440 GO:0034613 GO:0036211 GO:0042579 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044255 GO:0044260 GO:0044267 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044425 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046907 GO:0048598 GO:0048856 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0055085 GO:0055114 GO:0065002 GO:0070647 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0098588 GO:0098805 GO:0140096 GO:1901564 GO:1901575 GO:1990351 GO:1990415 GO:1990429
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
Pex2_Pex12 zf-C3HC4_2

Protein Analysis

304

Amino Acids

34.53

Weight (kDa)

9.27

Isoelectric Point (pI)

46.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pex2_Pex12 PF04757 19 - 214 9.2e-26 Pex2 / Pex12 amino terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 513
Acc16I TGCGCA 1 cut(s) 582
Acc65I GGTACC 1 cut(s) 633
AccB1I GGYRCC 1 cut(s) 633
AccII CGCG 2 cut(s) 771, 773
AciI CCGC 1 cut(s) 689
AclI AACGTT 1 cut(s) 879
AclWI GGATC 1 cut(s) 289
AcsI RAATTY 2 cut(s) 44, 474
AcuI CTGAAG 1 cut(s) 666
AfaI GTAC 2 cut(s) 559, 635
AfeI AGCGCT 1 cut(s) 851
AfiI CCNNNNNNNGG 1 cut(s) 717
AflIII ACRYGT 1 cut(s) 278
AgsI TTSAA 6 cut(s) 118, 246, 428, 480, 529, 829
AjnI CCWGG 2 cut(s) 570, 702
AloI GAACNNNNNNTCC 2 cut(s) 733, 765
AluBI AGCT 3 cut(s) 440, 454, 662
AluI AGCT 3 cut(s) 440, 454, 662
Alw26I GTCTC 2 cut(s) 64, 499
AlwI GGATC 1 cut(s) 289
AlwNI CAGNNNCTG 1 cut(s) 797
Ama87I CYCGRG 1 cut(s) 24
Aor51HI AGCGCT 1 cut(s) 851
ApeKI GCWGC 2 cut(s) 328, 599
ApoI RAATTY 2 cut(s) 44, 474
Asp718I GGTACC 1 cut(s) 633
AspLEI GCGC 4 cut(s) 418, 583, 773, 852
AspS9I GGNCC 1 cut(s) 515
AsuHPI GGTGA 3 cut(s) 40, 50, 203
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 515
BanI GGYRCC 1 cut(s) 633
BarI GAAGNNNNNNTAC 2 cut(s) 147, 179
BbvI GCAGC 2 cut(s) 315, 611
BccI CCATC 3 cut(s) 451, 616, 787
BceAI ACGGC 1 cut(s) 477
BciT130I CCWGG 2 cut(s) 572, 704
BciVI GTATCC 1 cut(s) 454
BcoDI GTCTC 2 cut(s) 64, 499
BfaI CTAG 2 cut(s) 336, 471
BfoI RGCGCY 1 cut(s) 853
BfuI GTATCC 1 cut(s) 454
BisI GCNGC 2 cut(s) 329, 600
BlsI GCNGC 2 cut(s) 330, 601
Bme1390I CCNGG 2 cut(s) 572, 704
Bme18I GGWCC 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 1 cut(s) 515
BmiI GGNNCC 1 cut(s) 635
BmrFI CCNGG 2 cut(s) 572, 704
BmrI ACTGGG 1 cut(s) 453
BmsI GCATC 3 cut(s) 275, 373, 858
BmuI ACTGGG 1 cut(s) 453
BplI GAGNNNNNCTC 2 cut(s) 534, 566
BsaJI CCNNGG 3 cut(s) 323, 521, 702
Bsc4I CCNNNNNNNGG 1 cut(s) 717
Bse1I ACTGG 3 cut(s) 448, 592, 857
Bse3DI GCAATG 1 cut(s) 545
BseBI CCWGG 2 cut(s) 572, 704
BseDI CCNNGG 3 cut(s) 323, 521, 702
BseGI GGATG 2 cut(s) 262, 388
BseLI CCNNNNNNNGG 1 cut(s) 717
BseMI GCAATG 1 cut(s) 545
BseMII CTCAG 3 cut(s) 99, 565, 854
BseNI ACTGG 3 cut(s) 448, 592, 857
BseXI GCAGC 2 cut(s) 315, 611
BsgI GTGCAG 1 cut(s) 29
Bsh1236I CGCG 2 cut(s) 771, 773
BshNI GGYRCC 1 cut(s) 633
BsiHKCI CYCGRG 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 717
BsmAI GTCTC 2 cut(s) 64, 499
BsoBI CYCGRG 1 cut(s) 24
Bsp143I GATC 1 cut(s) 294
Bsp19I CCATGG 2 cut(s) 323, 521
BspACI CCGC 1 cut(s) 689
BspCNI CTCAG 3 cut(s) 98, 564, 853
BspFNI CGCG 2 cut(s) 771, 773
BspHI TCATGA 1 cut(s) 904
BspLI GGNNCC 1 cut(s) 635
BspPI GGATC 1 cut(s) 289
BspT107I GGYRCC 1 cut(s) 633
BsrDI GCAATG 1 cut(s) 545
BsrI ACTGG 3 cut(s) 448, 592, 857
BssECI CCNNGG 3 cut(s) 323, 521, 702
BssMI GATC 1 cut(s) 294
BssT1I CCWWGG 2 cut(s) 323, 521
Bst2UI CCWGG 2 cut(s) 572, 704
Bst4CI ACNGT 2 cut(s) 568, 793
Bst6I CTCTTC 1 cut(s) 642
BstC8I GCNNGC 6 cut(s) 132, 230, 316, 333, 820, 873
BstDEI CTNAG 4 cut(s) 34, 85, 551, 840
BstDSI CCRYGG 2 cut(s) 323, 521
BstF5I GGATG 2 cut(s) 262, 388
BstFNI CGCG 2 cut(s) 771, 773
BstH2I RGCGCY 1 cut(s) 853
BstHHI GCGC 4 cut(s) 418, 583, 773, 852
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 2 cut(s) 64, 499
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 1 cut(s) 819
BstNI CCWGG 2 cut(s) 572, 704
BstNSI RCATGY 2 cut(s) 335, 416
BstSCI CCNGG 2 cut(s) 570, 702
BstUI CGCG 2 cut(s) 771, 773
BstV1I GCAGC 2 cut(s) 315, 611
BstX2I RGATCY 1 cut(s) 294
BstYI RGATCY 1 cut(s) 294
BsuI GTATCC 1 cut(s) 454
BtgI CCRYGG 2 cut(s) 323, 521
BtsCI GGATG 2 cut(s) 262, 388
BtsI GCAGTG 1 cut(s) 725
BtsIMutI CAGTG 3 cut(s) 276, 559, 725
Cac8I GCNNGC 6 cut(s) 132, 230, 316, 333, 820, 873
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 1 cut(s) 904
CfoI GCGC 4 cut(s) 418, 583, 773, 852
Cfr13I GGNCC 1 cut(s) 515
Csp6I GTAC 2 cut(s) 558, 634
CviAII CATG 5 cut(s) 324, 332, 413, 522, 905
CviJI RGCY 6 cut(s) 328, 440, 454, 492, 662, 898
CviKI_1 RGCY 6 cut(s) 328, 440, 454, 492, 662, 898
CviQI GTAC 2 cut(s) 558, 634
DdeI CTNAG 4 cut(s) 34, 85, 551, 840
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
DraI TTTAAA 1 cut(s) 616
DrdI GACNNNNNNGTC 1 cut(s) 513
DseDI GACNNNNNNGTC 1 cut(s) 513
Eam1104I CTCTTC 1 cut(s) 642
EarI CTCTTC 1 cut(s) 642
Eco130I CCWWGG 2 cut(s) 323, 521
Eco47I GGWCC 1 cut(s) 515
Eco47III AGCGCT 1 cut(s) 851
Eco57I CTGAAG 1 cut(s) 666
Eco88I CYCGRG 1 cut(s) 24
EcoRII CCWGG 2 cut(s) 570, 702
EcoT14I CCWWGG 2 cut(s) 323, 521
ErhI CCWWGG 2 cut(s) 323, 521
FaeI CATG 5 cut(s) 327, 335, 416, 525, 908
FatI CATG 5 cut(s) 323, 331, 412, 521, 904
Fnu4HI GCNGC 2 cut(s) 329, 600
FokI GGATG 2 cut(s) 269, 395
Fsp4HI GCNGC 2 cut(s) 329, 600
FspBI CTAG 2 cut(s) 336, 471
FspI TGCGCA 1 cut(s) 582
GlaI GCGC 4 cut(s) 417, 582, 772, 851
GluI GCNGC 2 cut(s) 329, 600
HaeII RGCGCY 1 cut(s) 853
HhaI GCGC 4 cut(s) 418, 583, 773, 852
Hin1II CATG 5 cut(s) 327, 335, 416, 525, 908
Hin6I GCGC 4 cut(s) 416, 581, 771, 850
HinP1I GCGC 4 cut(s) 416, 581, 771, 850
HincII GTYRAC 1 cut(s) 883
HindII GTYRAC 1 cut(s) 883
HinfI GANTC 4 cut(s) 20, 424, 721, 775
HphI GGTGA 3 cut(s) 40, 50, 203
Hpy166II GTNNAC 4 cut(s) 31, 497, 761, 883
Hpy188I TCNGA 1 cut(s) 736
Hpy188III TCNNGA 4 cut(s) 246, 298, 471, 905
Hpy8I GTNNAC 4 cut(s) 31, 497, 761, 883
Hpy99I CGWCG 1 cut(s) 391
HpyAV CCTTC 6 cut(s) 7, 338, 523, 705, 741, 888
HpyCH4III ACNGT 2 cut(s) 568, 793
HpyCH4IV ACGT 3 cut(s) 280, 499, 879
HpyCH4V TGCA 4 cut(s) 10, 130, 331, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 819
HpyF3I CTNAG 4 cut(s) 34, 85, 551, 840
HpySE526I ACGT 3 cut(s) 280, 499, 879
Hsp92II CATG 5 cut(s) 327, 335, 416, 525, 908
HspAI GCGC 4 cut(s) 416, 581, 771, 850
KpnI GGTACC 1 cut(s) 637
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 2 cut(s) 542, 667
Lsp1109I GCAGC 2 cut(s) 315, 611
LweI GCATC 3 cut(s) 275, 373, 858
MaeI CTAG 2 cut(s) 336, 471
MaeII ACGT 3 cut(s) 280, 499, 879
MaeIII GTNAC 1 cut(s) 859
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 3 cut(s) 304, 459, 659
MflI RGATCY 1 cut(s) 294
MluCI AATT 7 cut(s) 44, 125, 216, 302, 474, 594, 786
MmeI TCCRAC 3 cut(s) 42, 360, 689
MnlI CCTC 6 cut(s) 267, 528, 693, 702, 709, 762
MseI TTAA 2 cut(s) 593, 615
MslI CAYNNNNRTG 1 cut(s) 866
MspR9I CCNGG 2 cut(s) 572, 704
MvaI CCWGG 2 cut(s) 572, 704
MvnI CGCG 2 cut(s) 771, 773
MwoI GCNNNNNNNGC 1 cut(s) 819
NcoI CCATGG 2 cut(s) 323, 521
NdeII GATC 1 cut(s) 294
NlaIII CATG 5 cut(s) 327, 335, 416, 525, 908
NlaIV GGNNCC 1 cut(s) 635
NmuCI GTSAC 1 cut(s) 859
NsbI TGCGCA 1 cut(s) 582
NspI RCATGY 2 cut(s) 335, 416
PaeI GCATGC 1 cut(s) 335
PagI TCATGA 1 cut(s) 904
PfeI GAWTC 4 cut(s) 20, 424, 721, 775
PfoI TCCNGGA 1 cut(s) 570
PkrI GCNGC 2 cut(s) 330, 601
Psp1406I AACGTT 1 cut(s) 879
Psp6I CCWGG 2 cut(s) 570, 702
PspGI CCWGG 2 cut(s) 570, 702
PspN4I GGNNCC 1 cut(s) 635
PspPI GGNCC 1 cut(s) 515
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 1 cut(s) 294
RsaI GTAC 2 cut(s) 559, 635
RsaNI GTAC 2 cut(s) 558, 634
RseI CAYNNNNRTG 1 cut(s) 866
SaqAI TTAA 2 cut(s) 593, 615
SatI GCNGC 2 cut(s) 329, 600
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 1 cut(s) 515
ScrFI CCNGG 2 cut(s) 572, 704
SfaNI GCATC 3 cut(s) 275, 373, 858
SinI GGWCC 1 cut(s) 515
SmiMI CAYNNNNRTG 1 cut(s) 866
SphI GCATGC 1 cut(s) 335
Sse9I AATT 7 cut(s) 44, 125, 216, 302, 474, 594, 786
SsiI CCGC 1 cut(s) 689
SspMI CTAG 2 cut(s) 336, 471
StyD4I CCNGG 2 cut(s) 570, 702
StyI CCWWGG 2 cut(s) 323, 521
TaaI ACNGT 2 cut(s) 568, 793
TaiI ACGT 3 cut(s) 283, 502, 882
TaqI TCGA 2 cut(s) 122, 436
TasI AATT 7 cut(s) 44, 125, 216, 302, 474, 594, 786
TfiI GAWTC 4 cut(s) 20, 424, 721, 775
Tru1I TTAA 2 cut(s) 593, 615
Tru9I TTAA 2 cut(s) 593, 615
TscAI CASTG 3 cut(s) 276, 559, 732
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 2 cut(s) 328, 599
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 5 cut(s) 17, 38, 57, 342, 893
TspRI CASTG 3 cut(s) 276, 559, 732
VpaK11BI GGWCC 1 cut(s) 515
XapI RAATTY 2 cut(s) 44, 474
XbaI TCTAGA 1 cut(s) 470
XceI RCATGY 2 cut(s) 335, 416
XspI CTAG 2 cut(s) 336, 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.