AT3G04830

ER membrane protein complex subunit 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0007788
Physical Location & Seq
Forward (+)
1326121 .. 1329364
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G04830.1

Sequence Viewer

Length: 912 bp
ATGGTGACGAAAACGGAGGAGATACAGTTAAACCAACTCGAGAATCAAGTTGAGAATGGAGGAGGAGGTGTTTGGGAGTATCTGTGCCTCGTTCGTAAGCTCAAGGTTCGCCGATCAGAGATTGTGCTCAAGCATGGTCTCTCGATCTTGAACGATTCGGGAAAGCGATCCGCTCTTGGTCCAGATGAATGGACCCTGTATGAGCAGGTAGCAATTGCAGCTATGGACTGTCAATCTCTCGGTGTTGCACAGAATTGCATCAAGGTTCTAAAGAAGAAATTTCCGGAGAGCAAACGTGTTGGCAAGCTGGAGGCTCTGCTGCTAGAAGCAAAGGGAATGTGGGAAGAGGCTGAAAAAGCATATACAAGCCTTTTGGAGGATAATCCACTTGATCAAGTAATTCACAAAAGAAAGGTGGCTATGGCCAAGGCACAAGGCAAATCTTCCTTAGCCATTGAACATCTGAACAAGTATCTTGAAGTATTCATGGCTGATCATGATGCCTGGAGAGAACTTGCAGAAATTTATGTTTCCTTGCAAATGTACAAGCAAGCAGCTTTCTGCTACGAGGAGCTCATACTAACTCAGCCTACTCTTCCATTGTACCACTTAGCATATGCCGATGTTCTCTACACAATAGGTGGACTAGAAAACCTCATCGCAGCAAGAAAGTACTATGCAGCAACTATAGACTTGACAGGTGGTAAAAGCACAAGAGCACTTCTCGGAATATGCTTGTGTGGATCAGCAATCGCACAGATCTCAAAAGGCAGGAACAAAGAGGACAAGGACATGGCTGCACCAGAGCTTCAGTCTTTGGCTGCAACTGCATTGGAGCGAGAGTACAAGCAAAAAGCTCCCGCTAAGCTCAACCTCCTCACTTCTGCCTTGAGAAACTTGAAAATCGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19

Protein Analysis

303

Amino Acids

33.74

Weight (kDa)

8.37

Isoelectric Point (pI)

41.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_EMC2 PF22890 100 - 209 5.8e-58 EMC2 TPR-like repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 173
AccIII TCCGGA 1 cut(s) 283
AciI CCGC 2 cut(s) 171, 861
AclWI GGATC 2 cut(s) 162, 751
AcoI YGGCCR 1 cut(s) 423
AcsI RAATTY 2 cut(s) 278, 522
AcuI CTGAAG 1 cut(s) 794
AfaI GTAC 4 cut(s) 545, 605, 674, 845
AfiI CCNNNNNNNGG 1 cut(s) 376
AflIII ACRYGT 1 cut(s) 295
AgsI TTSAA 4 cut(s) 151, 458, 479, 901
AjnI CCWGG 1 cut(s) 503
AluBI AGCT 8 cut(s) 100, 221, 307, 557, 574, 808, 857, 868
AluI AGCT 8 cut(s) 100, 221, 307, 557, 574, 808, 857, 868
Alw21I GWGCWC 3 cut(s) 129, 576, 721
Alw26I GTCTC 1 cut(s) 143
AlwI GGATC 2 cut(s) 162, 751
Ama87I CYCGRG 1 cut(s) 38
Aor13HI TCCGGA 1 cut(s) 283
AoxI GGCC 1 cut(s) 423
ApeKI GCWGC 7 cut(s) 218, 319, 554, 662, 680, 797, 821
ApoI RAATTY 2 cut(s) 278, 522
AspS9I GGNCC 2 cut(s) 179, 192
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 2 cut(s) 179, 192
BalI TGGCCA 1 cut(s) 425
BanII GRGCYC 1 cut(s) 576
Bbv12I GWGCWC 3 cut(s) 129, 576, 721
BbvI GCAGC 7 cut(s) 230, 306, 566, 674, 692, 784, 808
BciT130I CCWGG 1 cut(s) 505
BclI TGATCA 2 cut(s) 391, 493
BcoDI GTCTC 1 cut(s) 143
BfaI CTAG 2 cut(s) 323, 647
BfmI CTRYAG 1 cut(s) 687
BfuAI ACCTGC 1 cut(s) 196
BglII AGATCT 1 cut(s) 759
BisI GCNGC 7 cut(s) 219, 320, 555, 663, 681, 798, 822
BlpI GCTNAGC 1 cut(s) 864
BlsI GCNGC 7 cut(s) 220, 321, 556, 664, 682, 799, 823
BmcAI AGTACT 1 cut(s) 674
Bme1390I CCNGG 1 cut(s) 505
Bme18I GGWCC 2 cut(s) 179, 192
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 179, 192
BmiI GGNNCC 1 cut(s) 194
BmrFI CCNGG 1 cut(s) 505
BmsI GCATC 2 cut(s) 267, 490
BplI GAGNNNNNCTC 2 cut(s) 708, 740
BpmI CTGGAG 2 cut(s) 329, 526
Bpu10I CCTNAGC 1 cut(s) 448
Bpu1102I GCTNAGC 1 cut(s) 864
BpuEI CTTGAG 3 cut(s) 86, 113, 910
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 1 cut(s) 426
BsaWI WCCGGW 1 cut(s) 283
BsaXI ACNNNNNCTCC 8 cut(s) 11, 41, 54, 68, 84, 98, 827, 857
Bsc4I CCNNNNNNNGG 1 cut(s) 376
BseAI TCCGGA 1 cut(s) 283
BseBI CCWGG 1 cut(s) 505
BseDI CCNNGG 1 cut(s) 426
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMII CTCAG 1 cut(s) 599
BseRI GAGGAG 5 cut(s) 32, 75, 78, 584, 866
BseXI GCAGC 7 cut(s) 230, 306, 566, 674, 692, 784, 808
BsgI GTGCAG 1 cut(s) 783
BshFI GGCC 1 cut(s) 425
BsiHKAI GWGCWC 3 cut(s) 129, 576, 721
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 284
BslI CCNNNNNNNGG 1 cut(s) 376
BsmAI GTCTC 1 cut(s) 143
BsnI GGCC 1 cut(s) 425
Bso31I GGTCTC 1 cut(s) 143
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 3 cut(s) 129, 576, 721
Bsp13I TCCGGA 1 cut(s) 283
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
Bsp1720I GCTNAGC 1 cut(s) 864
BspACI CCGC 2 cut(s) 171, 861
BspANI GGCC 1 cut(s) 425
BspCNI CTCAG 1 cut(s) 598
BspEI TCCGGA 1 cut(s) 283
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 1 cut(s) 194
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 2 cut(s) 162, 751
BspTNI GGTCTC 1 cut(s) 143
BsrBI CCGCTC 1 cut(s) 173
BsrGI TGTACA 1 cut(s) 543
BssECI CCNNGG 1 cut(s) 426
BssMI GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
BssT1I CCWWGG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 505
Bst4CI ACNGT 2 cut(s) 27, 230
Bst6I CTCTTC 2 cut(s) 339, 600
BstAUI TGTACA 1 cut(s) 543
BstC8I GCNNGC 2 cut(s) 305, 552
BstDEI CTNAG 5 cut(s) 448, 585, 610, 864, 909
BstENI CCTNNNNNAGG 1 cut(s) 374
BstKTI GATC 7 cut(s) 116, 147, 170, 394, 496, 746, 762
BstMAI GTCTC 1 cut(s) 143
BstMBI GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
BstMWI GCNNNNNNNGC 3 cut(s) 218, 356, 827
BstNI CCWGG 1 cut(s) 505
BstSCI CCNGG 1 cut(s) 503
BstSFI CTRYAG 1 cut(s) 687
BstV1I GCAGC 7 cut(s) 230, 306, 566, 674, 692, 784, 808
BstX2I RGATCY 1 cut(s) 759
BstXI CCANNNNNNTGG 1 cut(s) 189
BstYI RGATCY 1 cut(s) 759
BsuRI GGCC 1 cut(s) 425
BtgZI GCGATG 1 cut(s) 643
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 305, 552
CciI TCATGA 1 cut(s) 496
Cfr13I GGNCC 2 cut(s) 179, 192
Csp6I GTAC 4 cut(s) 544, 604, 673, 844
CviAII CATG 4 cut(s) 134, 487, 497, 793
CviQI GTAC 4 cut(s) 544, 604, 673, 844
DdeI CTNAG 5 cut(s) 448, 585, 610, 864, 909
DpnI GATC 7 cut(s) 115, 146, 169, 393, 495, 745, 761
DpnII GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
EaeI YGGCCR 1 cut(s) 423
Eam1104I CTCTTC 2 cut(s) 339, 600
EarI CTCTTC 2 cut(s) 339, 600
Ecl136II GAGCTC 1 cut(s) 574
Eco130I CCWWGG 1 cut(s) 426
Eco24I GRGCYC 1 cut(s) 576
Eco31I GGTCTC 1 cut(s) 143
Eco47I GGWCC 2 cut(s) 179, 192
Eco53kI GAGCTC 1 cut(s) 574
Eco57I CTGAAG 1 cut(s) 794
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 574
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoRII CCWGG 1 cut(s) 503
EcoT14I CCWWGG 1 cut(s) 426
EcoT38I GRGCYC 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 426
FaeI CATG 4 cut(s) 137, 490, 500, 796
FatI CATG 4 cut(s) 133, 486, 496, 792
FauI CCCGC 1 cut(s) 868
FauNDI CATATG 1 cut(s) 616
FbaI TGATCA 2 cut(s) 391, 493
Fnu4HI GCNGC 7 cut(s) 219, 320, 555, 663, 681, 798, 822
FriOI GRGCYC 1 cut(s) 576
Fsp4HI GCNGC 7 cut(s) 219, 320, 555, 663, 681, 798, 822
FspBI CTAG 2 cut(s) 323, 647
GluI GCNGC 7 cut(s) 219, 320, 555, 663, 681, 798, 822
GsuI CTGGAG 2 cut(s) 329, 526
HaeIII GGCC 1 cut(s) 425
HapII CCGG 1 cut(s) 284
Hin1II CATG 4 cut(s) 137, 490, 500, 796
HinfI GANTC 2 cut(s) 43, 155
HpaII CCGG 1 cut(s) 284
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 1 cut(s) 644
Hpy188I TCNGA 3 cut(s) 118, 465, 728
Hpy188III TCNNGA 8 cut(s) 40, 142, 148, 159, 182, 284, 476, 497
Hpy8I GTNNAC 1 cut(s) 644
HpyCH4III ACNGT 2 cut(s) 27, 230
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 9 cut(s) 218, 248, 258, 518, 538, 680, 800, 824, 830
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 356, 827
HpyF3I CTNAG 5 cut(s) 448, 585, 610, 864, 909
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 4 cut(s) 137, 490, 500, 796
Kpn2I TCCGGA 1 cut(s) 283
Ksp22I TGATCA 2 cut(s) 391, 493
Kzo9I GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
LmnI GCTCC 3 cut(s) 571, 835, 862
Lsp1109I GCAGC 7 cut(s) 230, 306, 566, 674, 692, 784, 808
LweI GCATC 2 cut(s) 267, 490
MaeI CTAG 2 cut(s) 323, 647
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 1 cut(s) 4
MalI GATC 7 cut(s) 115, 146, 169, 393, 495, 745, 761
MbiI CCGCTC 1 cut(s) 173
MboI GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
MboII GAAGA 4 cut(s) 286, 356, 435, 587
MfeI CAATTG 1 cut(s) 213
MflI RGATCY 1 cut(s) 759
MhlI GDGCHC 3 cut(s) 129, 576, 721
MlsI TGGCCA 1 cut(s) 425
MluCI AATT 5 cut(s) 213, 253, 278, 399, 522
MluNI TGGCCA 1 cut(s) 425
Mox20I TGGCCA 1 cut(s) 425
MroI TCCGGA 1 cut(s) 283
MscI TGGCCA 1 cut(s) 425
MseI TTAA 1 cut(s) 29
Msp20I TGGCCA 1 cut(s) 425
MspI CCGG 1 cut(s) 284
MspR9I CCNGG 1 cut(s) 505
MunI CAATTG 1 cut(s) 213
MvaI CCWGG 1 cut(s) 505
MwoI GCNNNNNNNGC 3 cut(s) 218, 356, 827
NdeI CATATG 1 cut(s) 616
NdeII GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
NlaIII CATG 4 cut(s) 137, 490, 500, 796
NlaIV GGNNCC 1 cut(s) 194
NmuCI GTSAC 1 cut(s) 4
PaeR7I CTCGAG 1 cut(s) 38
PagI TCATGA 1 cut(s) 496
PfeI GAWTC 2 cut(s) 43, 155
PkrI GCNGC 7 cut(s) 220, 321, 556, 664, 682, 799, 823
Psp124BI GAGCTC 1 cut(s) 576
Psp6I CCWGG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 503
PspN4I GGNNCC 1 cut(s) 194
PspPI GGNCC 2 cut(s) 179, 192
PsuI RGATCY 1 cut(s) 759
RsaI GTAC 4 cut(s) 545, 605, 674, 845
RsaNI GTAC 4 cut(s) 544, 604, 673, 844
SacI GAGCTC 1 cut(s) 576
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 7 cut(s) 219, 320, 555, 663, 681, 798, 822
Sau3AI GATC 7 cut(s) 113, 144, 167, 391, 493, 743, 759
Sau96I GGNCC 2 cut(s) 179, 192
ScaI AGTACT 1 cut(s) 674
ScrFI CCNGG 1 cut(s) 505
SduI GDGCHC 3 cut(s) 129, 576, 721
SfaNI GCATC 2 cut(s) 267, 490
SfcI CTRYAG 1 cut(s) 687
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 2 cut(s) 179, 192
SlaI CTCGAG 1 cut(s) 38
SmlI CTYRAG 4 cut(s) 38, 101, 128, 889
SmoI CTYRAG 4 cut(s) 38, 101, 128, 889
Sse9I AATT 5 cut(s) 213, 253, 278, 399, 522
SsiI CCGC 2 cut(s) 171, 861
SspMI CTAG 2 cut(s) 323, 647
SstI GAGCTC 1 cut(s) 576
StyD4I CCNGG 1 cut(s) 503
StyI CCWWGG 1 cut(s) 426
TaaI ACNGT 2 cut(s) 27, 230
TaiI ACGT 1 cut(s) 298
TaqI TCGA 2 cut(s) 39, 143
TasI AATT 5 cut(s) 213, 253, 278, 399, 522
TatI WGTACW 3 cut(s) 543, 672, 843
TfiI GAWTC 2 cut(s) 43, 155
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TseFI GTSAC 1 cut(s) 4
TseI GCWGC 7 cut(s) 218, 319, 554, 662, 680, 797, 821
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 201, 475
TspGWI ACGGA 1 cut(s) 29
VpaK11BI GGWCC 2 cut(s) 179, 192
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 2 cut(s) 278, 522
XhoI CTCGAG 1 cut(s) 38
XspI CTAG 2 cut(s) 323, 647
ZrmI AGTACT 1 cut(s) 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.