MD04G1221600.v1.1

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0007788
Physical Location & Seq
Reverse (-)
30283085 .. 30286326
3242 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1221600.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGTGAGCAAAACGGAAGAAACCCAATTGAACAGGCTCGAGAACCAGGTCGACAATGGAGGAGGAGGGGCCTGGGAGTACCTCTGTTTAGTCAGAAAGCTCAAAGTCCGCCGCTCCGAGAAGGTCTTGAAGTACGGCCTGTCGATCTTGAACGACCTCAAGAAAAGATCCGGTCTTGGCTCGGAAGAATGGACTTTATATGAGCAAGTTGCAATTGCAGCTATGGACTGCCAGTCTCTTGATGTTGCAAAGGACTGCATTAGGCTTTTACGTAAGGCATTTCCTGAGAGTAAAAGAGTTGGCAGGCTAGAGGCTATGTTGCTAGAGGCAAAGGGATCTTGGGCAGAGGCAGAGAAGGCTTACTCAAGCTTTCTGGAGGAAAATCCACTCGATCAAGTGATACATAAGAGAAGAGTAGCTATGGCAAAGGCACAAGGATACATTTCAGGGGCCATTGAGGGGCTTAATAAATATCTGGAAATATTTATGGCTGACCATGATGCGTGGAGAGAGCTGGCTGAAATCTACGTTTCCTTGCAAATGTACAAGCAAGCTGCGTTCTGCTATGAGGAGCTGATATTATCACAACCTACTGTTCCTCTTCACCACTTGGCTTATGCTGATGTGCTATACACGCTTGGTGGACTAGAAAACCTTCAGACAGCAAAAAAATACTACGCATCCGTTATAGACTTGACTGGGGGCAAGAATACCAGAGCACTTTTTGGTATTAACTTGTGCACTTCCGCCATCGGACAGCTTGCGAAAGGAAGGAACAAGGAAGACAAAGAGAGCTTAGAGCTACAATCACTGGCAGCAACGGCCTTGGAGAAAGACTACAAGCAGAGAGCACCCGAAAAACTTTCCCTGCTTACGACGGCCTTGAAAAGCTTGAAAGTTTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_19

Protein Analysis

303

Amino Acids

33.82

Weight (kDa)

8.41

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_EMC2 PF22890 100 - 209 2.4e-56 EMC2 TPR-like repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 114
AccI GTMKAC 1 cut(s) 51
AciI CCGC 3 cut(s) 109, 112, 747
AclWI GGATC 2 cut(s) 162, 343
AcuI CTGAAG 1 cut(s) 641
AfaI GTAC 3 cut(s) 80, 134, 545
AfiI CCNNNNNNNGG 1 cut(s) 459
AgsI TTSAA 5 cut(s) 31, 130, 151, 886, 895
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 45, 71
Alw21I GWGCWC 3 cut(s) 721, 743, 853
Alw26I GTCTC 1 cut(s) 240
Alw44I GTGCAC 1 cut(s) 739
AlwI GGATC 2 cut(s) 162, 343
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 5 cut(s) 69, 136, 450, 822, 879
ApaLI GTGCAC 1 cut(s) 739
ApeKI GCWGC 3 cut(s) 218, 554, 815
Asp700I GAANNNNTTC 1 cut(s) 654
AspS9I GGNCC 2 cut(s) 69, 450
AsuHPI GGTGA 2 cut(s) 16, 596
AvaI CYCGRG 1 cut(s) 38
BaeGI GKGCMC 1 cut(s) 743
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 3 cut(s) 721, 743, 853
BbvI GCAGC 3 cut(s) 230, 541, 827
BccI CCATC 1 cut(s) 758
BceAI ACGGC 3 cut(s) 151, 837, 894
BciT130I CCWGG 2 cut(s) 47, 73
BciVI GTATCC 1 cut(s) 431
BcoDI GTCTC 1 cut(s) 240
BfaI CTAG 3 cut(s) 308, 323, 647
BfuI GTATCC 1 cut(s) 431
BisI GCNGC 4 cut(s) 112, 219, 555, 816
BlsI GCNGC 4 cut(s) 113, 220, 556, 817
Bme1390I CCNGG 2 cut(s) 47, 73
BmeRI GACNNNNNGTC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 2 cut(s) 69, 450
BmiI GGNNCC 2 cut(s) 70, 451
BmrFI CCNGG 2 cut(s) 47, 73
BmrI ACTGGG 1 cut(s) 708
BmsI GCATC 2 cut(s) 490, 689
BmuI ACTGGG 1 cut(s) 708
BpiI GAAGAC 1 cut(s) 789
BpmI CTGGAG 1 cut(s) 395
BpuEI CTTGAG 2 cut(s) 143, 349
BsaAI YACGTR 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 72, 825
BsaWI WCCGGW 1 cut(s) 170
Bsc4I CCNNNNNNNGG 1 cut(s) 459
Bse1I ACTGG 3 cut(s) 232, 703, 816
BseBI CCWGG 2 cut(s) 47, 73
BseDI CCNNGG 2 cut(s) 72, 825
BseGI GGATG 1 cut(s) 680
BseLI CCNNNNNNNGG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 276
BseNI ACTGG 3 cut(s) 232, 703, 816
BseRI GAGGAG 3 cut(s) 75, 78, 584
BseSI GKGCMC 1 cut(s) 743
BseXI GCAGC 3 cut(s) 230, 541, 827
BshFI GGCC 5 cut(s) 71, 138, 452, 824, 881
BsiHKAI GWGCWC 3 cut(s) 721, 743, 853
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 171
BslI CCNNNNNNNGG 1 cut(s) 459
BsmAI GTCTC 1 cut(s) 240
BsnI GGCC 5 cut(s) 71, 138, 452, 824, 881
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 3 cut(s) 721, 743, 853
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 4 cut(s) 144, 167, 335, 391
BspACI CCGC 3 cut(s) 109, 112, 747
BspANI GGCC 5 cut(s) 71, 138, 452, 824, 881
BspCNI CTCAG 1 cut(s) 277
BspLI GGNNCC 2 cut(s) 70, 451
BspPI GGATC 2 cut(s) 162, 343
BsrBI CCGCTC 1 cut(s) 114
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 3 cut(s) 232, 703, 816
BssECI CCNNGG 2 cut(s) 72, 825
BssMI GATC 4 cut(s) 144, 167, 335, 391
BssT1I CCWWGG 1 cut(s) 825
Bst2UI CCWGG 2 cut(s) 47, 73
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 2 cut(s) 406, 606
BstAUI TGTACA 1 cut(s) 543
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 4 cut(s) 305, 516, 552, 762
BstDEI CTNAG 2 cut(s) 285, 796
BstF5I GGATG 1 cut(s) 680
BstKTI GATC 4 cut(s) 147, 170, 338, 394
BstMAI GTCTC 1 cut(s) 240
BstMBI GATC 4 cut(s) 144, 167, 335, 391
BstMWI GCNNNNNNNGC 4 cut(s) 218, 356, 634, 821
BstNI CCWGG 2 cut(s) 47, 73
BstSCI CCNGG 2 cut(s) 45, 71
BstSLI GKGCMC 1 cut(s) 743
BstSNI TACGTA 1 cut(s) 272
BstV1I GCAGC 3 cut(s) 230, 541, 827
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 2 cut(s) 167, 335
BstYI RGATCY 2 cut(s) 167, 335
BsuI GTATCC 1 cut(s) 431
BsuRI GGCC 5 cut(s) 71, 138, 452, 824, 881
BtsCI GGATG 1 cut(s) 680
BtsIMutI CAGTG 1 cut(s) 809
Cac8I GCNNGC 4 cut(s) 305, 516, 552, 762
Cfr13I GGNCC 2 cut(s) 69, 450
CsiI ACCWGGT 1 cut(s) 45
Csp6I GTAC 3 cut(s) 79, 133, 544
CviAII CATG 1 cut(s) 497
CviQI GTAC 3 cut(s) 79, 133, 544
DdeI CTNAG 2 cut(s) 285, 796
DpnI GATC 4 cut(s) 146, 169, 337, 393
DpnII GATC 4 cut(s) 144, 167, 335, 391
DriI GACNNNNNGTC 1 cut(s) 232
Eam1104I CTCTTC 2 cut(s) 406, 606
Eam1105I GACNNNNNGTC 1 cut(s) 232
EarI CTCTTC 2 cut(s) 406, 606
EciI GGCGGA 2 cut(s) 98, 736
Eco105I TACGTA 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 825
Eco57I CTGAAG 1 cut(s) 641
Eco88I CYCGRG 1 cut(s) 38
EcoO109I RGGNCCY 1 cut(s) 69
EcoRII CCWGG 2 cut(s) 45, 71
EcoT14I CCWWGG 1 cut(s) 825
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 1 cut(s) 500
FatI CATG 1 cut(s) 496
FblI GTMKAC 1 cut(s) 51
Fnu4HI GCNGC 4 cut(s) 112, 219, 555, 816
FokI GGATG 1 cut(s) 667
Fsp4HI GCNGC 4 cut(s) 112, 219, 555, 816
FspBI CTAG 3 cut(s) 308, 323, 647
GluI GCNGC 4 cut(s) 112, 219, 555, 816
GsuI CTGGAG 1 cut(s) 395
HaeIII GGCC 5 cut(s) 71, 138, 452, 824, 881
HapII CCGG 1 cut(s) 171
Hin1II CATG 1 cut(s) 500
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 2 cut(s) 367, 889
HpaII CCGG 1 cut(s) 171
HphI GGTGA 2 cut(s) 16, 596
Hpy166II GTNNAC 3 cut(s) 52, 644, 741
Hpy188I TCNGA 5 cut(s) 95, 118, 184, 660, 755
Hpy188III TCNNGA 8 cut(s) 40, 127, 148, 160, 239, 284, 374, 476
Hpy8I GTNNAC 3 cut(s) 52, 644, 741
Hpy99I CGWCG 1 cut(s) 880
HpyAV CCTTC 4 cut(s) 115, 349, 665, 765
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4IV ACGT 2 cut(s) 271, 528
HpyCH4V TGCA 6 cut(s) 212, 218, 248, 258, 538, 741
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 356, 634, 821
HpyF3I CTNAG 2 cut(s) 285, 796
HpySE526I ACGT 2 cut(s) 271, 528
Hsp92II CATG 1 cut(s) 500
Kzo9I GATC 4 cut(s) 144, 167, 335, 391
LmnI GCTCC 2 cut(s) 119, 571
Lsp1109I GCAGC 3 cut(s) 230, 541, 827
LweI GCATC 2 cut(s) 490, 689
MabI ACCWGGT 1 cut(s) 45
MaeI CTAG 3 cut(s) 308, 323, 647
MaeII ACGT 2 cut(s) 271, 528
MalI GATC 4 cut(s) 146, 169, 337, 393
MbiI CCGCTC 1 cut(s) 114
MboI GATC 4 cut(s) 144, 167, 335, 391
MboII GAAGA 5 cut(s) 29, 197, 423, 593, 794
MfeI CAATTG 2 cut(s) 26, 213
MflI RGATCY 2 cut(s) 167, 335
MhlI GDGCHC 3 cut(s) 721, 743, 853
MluCI AATT 2 cut(s) 26, 213
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 2 cut(s) 465, 732
MspI CCGG 1 cut(s) 171
MspR9I CCNGG 2 cut(s) 47, 73
MunI CAATTG 2 cut(s) 26, 213
MvaI CCWGG 2 cut(s) 47, 73
MwoI GCNNNNNNNGC 4 cut(s) 218, 356, 634, 821
NdeII GATC 4 cut(s) 144, 167, 335, 391
NlaIII CATG 1 cut(s) 500
NlaIV GGNNCC 2 cut(s) 70, 451
PaeR7I CTCGAG 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 654
PkrI GCNGC 4 cut(s) 113, 220, 556, 817
Ppu21I YACGTR 1 cut(s) 272
Psp6I CCWGG 2 cut(s) 45, 71
PspGI CCWGG 2 cut(s) 45, 71
PspN4I GGNNCC 2 cut(s) 70, 451
PspPI GGNCC 2 cut(s) 69, 450
PsuI RGATCY 2 cut(s) 167, 335
RsaI GTAC 3 cut(s) 80, 134, 545
RsaNI GTAC 3 cut(s) 79, 133, 544
SalI GTCGAC 1 cut(s) 50
SaqAI TTAA 2 cut(s) 465, 732
SatI GCNGC 4 cut(s) 112, 219, 555, 816
Sau3AI GATC 4 cut(s) 144, 167, 335, 391
Sau96I GGNCC 2 cut(s) 69, 450
ScrFI CCNGG 2 cut(s) 47, 73
SduI GDGCHC 3 cut(s) 721, 743, 853
SexAI ACCWGGT 1 cut(s) 45
SfaNI GCATC 2 cut(s) 490, 689
Sfr274I CTCGAG 1 cut(s) 38
SlaI CTCGAG 1 cut(s) 38
SmlI CTYRAG 3 cut(s) 38, 158, 364
SmoI CTYRAG 3 cut(s) 38, 158, 364
SnaBI TACGTA 1 cut(s) 272
Sse9I AATT 2 cut(s) 26, 213
SsiI CCGC 3 cut(s) 109, 112, 747
SspI AATATT 1 cut(s) 483
SspMI CTAG 3 cut(s) 308, 323, 647
StyD4I CCNGG 2 cut(s) 45, 71
StyI CCWWGG 1 cut(s) 825
TaaI ACNGT 1 cut(s) 595
TaiI ACGT 2 cut(s) 274, 531
TaqI TCGA 4 cut(s) 39, 51, 143, 390
TasI AATT 2 cut(s) 26, 213
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 114
Tru1I TTAA 2 cut(s) 465, 732
Tru9I TTAA 2 cut(s) 465, 732
TscAI CASTG 1 cut(s) 816
TseI GCWGC 3 cut(s) 218, 554, 815
TspDTI ATGAA 1 cut(s) 891
TspGWI ACGGA 2 cut(s) 29, 673
TspRI CASTG 1 cut(s) 816
VneI GTGCAC 1 cut(s) 739
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 38
XmiI GTMKAC 1 cut(s) 51
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 3 cut(s) 308, 323, 647
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.