AT5G28220

ER membrane protein complex subunit 2-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007788
Physical Location & Seq
Reverse (-)
10190357 .. 10193123
2767 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G28220.1

Sequence Viewer

Length: 951 bp
ATGCTCGTAAGTTCAAGCAAAATCACAAGAGCGAGAGAGATGGTGACGAAAACAGAGGAGAAGCAATTGAACCAGCTAGAGATTCAAGTCGATAATGGCGGAGGCGGAACATGGGAGTATCTTTGCCTCGTTCGTAATCTCAAAGTTCGCCGGTCGGAGAAAGTGTTAAAACACGGTTCCTCGATTTTGAATGATCAGAAGAAACGATCTGCTCTTGGTCCAGATGAATGGACACTAAATGAGCAGGTGGCAATTGCAGCTATGGACTGTCAATGTCTCGGTGTCGCACAGAGTTGCATTAAGGCTTTGCAGACAAAATTTCCTGGGAGCAAAAGAGTTGGGAGGCTTGAGGCATTGCTTCTTGAAGCGAAGGGATTATGGGGAGAGGCTGAGGAAGCATATGCGAGTCTTTTGGAGGATAATCCACTCGACCAAGCGATACACAAACGAAGAGTGGCTATATCCAAGGCACTAGGCAAACCTTCCATAGCCATTGAGCTTCTTAACAAATATCTTGAACTATTCATGGCTGATCATGATGCATGGAGAGAACTCGCAGAGCTTTACCTTTCCTTGCAAATGTATAAGCAAGCAGCTTTCTGCTATGAAGAGCTCATACTATCTCAGCCTACTGTTCCATTGTACCACCTCGCATATGCTGAGGTTCTCTATACAATCGGTGGAGTAGAAAACATTATCTCAGCAAGAAAATACTATGCAGCGACCGTAGATTTAACAGGCGGCAAAAACACTAGAGCTCTTCTCGGAATCTGCTTGTGTGCATCGGCCATTGCACAGCTCTCAAAAGGCAGGAACAAAGAGGACAAAGACGCTACGGCAGCCCCAGAGCTTCATTCCCTGGCTGCAGCTGCAGTAGAGAAAGAATACAAGCAAAAAGCCCCGGACAAACTTCAACTCATCTCTTCCGCGTTAAGAATCTTGAAGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19

Protein Analysis

316

Amino Acids

34.97

Weight (kDa)

8.53

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_EMC2 PF22890 113 - 222 2.5e-56 EMC2 TPR-like repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 235
AccII CGCG 1 cut(s) 929
AciI CCGC 4 cut(s) 99, 105, 741, 927
AcoI YGGCCR 1 cut(s) 786
AcsI RAATTY 1 cut(s) 317
AfaI GTAC 1 cut(s) 644
AgsI TTSAA 8 cut(s) 15, 70, 86, 190, 365, 518, 914, 943
AjnI CCWGG 2 cut(s) 322, 858
Alw21I GWGCWC 2 cut(s) 615, 760
Alw26I GTCTC 1 cut(s) 281
AoxI GGCC 1 cut(s) 786
ApeKI GCWGC 7 cut(s) 257, 593, 719, 839, 863, 866, 869
ApoI RAATTY 1 cut(s) 317
AspS9I GGNCC 1 cut(s) 218
AsuC2I CCSGG 1 cut(s) 902
AsuHPI GGTGA 1 cut(s) 55
AvaII GGWCC 1 cut(s) 218
BanII GRGCYC 2 cut(s) 615, 760
BarI GAAGNNNNNNTAC 2 cut(s) 600, 632
Bbv12I GWGCWC 2 cut(s) 615, 760
BbvCI CCTCAGC 2 cut(s) 390, 660
BbvI GCAGC 7 cut(s) 269, 605, 731, 850, 851, 856, 878
BccI CCATC 1 cut(s) 34
BceAI ACGGC 1 cut(s) 852
BciT130I CCWGG 2 cut(s) 324, 860
BclI TGATCA 2 cut(s) 193, 532
BcnI CCSGG 1 cut(s) 902
BcoDI GTCTC 1 cut(s) 281
BfaI CTAG 3 cut(s) 77, 473, 753
BfmI CTRYAG 2 cut(s) 864, 870
BfuAI ACCTGC 1 cut(s) 235
BisI GCNGC 8 cut(s) 258, 594, 720, 742, 840, 864, 867, 870
BlsI GCNGC 8 cut(s) 259, 595, 721, 743, 841, 865, 868, 871
Bme1390I CCNGG 3 cut(s) 324, 860, 902
Bme18I GGWCC 1 cut(s) 218
BmgT120I GGNCC 1 cut(s) 218
BmiI GGNNCC 1 cut(s) 178
BmrFI CCNGG 3 cut(s) 324, 860, 902
BmsI GCATC 2 cut(s) 529, 791
BplI GAGNNNNNCTC 2 cut(s) 747, 779
Bpu10I CCTNAGC 2 cut(s) 390, 660
BpuEI CTTGAG 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 902
BsaJI CCNNGG 4 cut(s) 323, 465, 858, 900
BsaXI ACNNNNNCTCC 2 cut(s) 149, 179
Bse118I RCCGGY 1 cut(s) 150
Bse3DI GCAATG 2 cut(s) 353, 789
BseBI CCWGG 2 cut(s) 324, 860
BseDI CCNNGG 4 cut(s) 323, 465, 858, 900
BseMI GCAATG 2 cut(s) 353, 789
BseMII CTCAG 4 cut(s) 381, 638, 651, 714
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 7 cut(s) 269, 605, 731, 850, 851, 856, 878
Bsh1236I CGCG 1 cut(s) 929
Bsh1285I CGRYCG 2 cut(s) 155, 726
BshFI GGCC 1 cut(s) 788
BsiEI CGRYCG 2 cut(s) 155, 726
BsiHKAI GWGCWC 2 cut(s) 615, 760
BsiSI CCGG 2 cut(s) 151, 902
BsmAI GTCTC 1 cut(s) 281
BsnI GGCC 1 cut(s) 788
Bsp1286I GDGCHC 2 cut(s) 615, 760
Bsp143I GATC 3 cut(s) 193, 206, 532
BspACI CCGC 4 cut(s) 99, 105, 741, 927
BspANI GGCC 1 cut(s) 788
BspCNI CTCAG 4 cut(s) 382, 637, 652, 713
BspFNI CGCG 1 cut(s) 929
BspHI TCATGA 1 cut(s) 535
BspLI GGNNCC 1 cut(s) 178
BspMAI CTGCAG 2 cut(s) 868, 874
BspMI ACCTGC 1 cut(s) 235
BspQI GCTCTTC 2 cut(s) 603, 765
BsrDI GCAATG 2 cut(s) 353, 789
BsrFI RCCGGY 1 cut(s) 150
BssAI RCCGGY 1 cut(s) 150
BssECI CCNNGG 4 cut(s) 323, 465, 858, 900
BssMI GATC 3 cut(s) 193, 206, 532
BssT1I CCWWGG 1 cut(s) 465
Bst2UI CCWGG 2 cut(s) 324, 860
Bst4CI ACNGT 4 cut(s) 176, 269, 634, 727
Bst6I CTCTTC 4 cut(s) 445, 603, 765, 928
BstC8I GCNNGC 1 cut(s) 591
BstDEI CTNAG 4 cut(s) 390, 624, 660, 700
BstFNI CGCG 1 cut(s) 929
BstKTI GATC 3 cut(s) 196, 209, 535
BstMAI GTCTC 1 cut(s) 281
BstMBI GATC 3 cut(s) 193, 206, 532
BstMCI CGRYCG 2 cut(s) 155, 726
BstMWI GCNNNNNNNGC 4 cut(s) 257, 395, 839, 869
BstNI CCWGG 2 cut(s) 324, 860
BstSCI CCNGG 3 cut(s) 322, 858, 900
BstSFI CTRYAG 2 cut(s) 864, 870
BstUI CGCG 1 cut(s) 929
BstV1I GCAGC 7 cut(s) 269, 605, 731, 850, 851, 856, 878
BstXI CCANNNNNNTGG 1 cut(s) 228
BsuRI GGCC 1 cut(s) 788
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 1 cut(s) 591
CciI TCATGA 1 cut(s) 535
Cfr10I RCCGGY 1 cut(s) 150
Cfr13I GGNCC 1 cut(s) 218
CseI GACGC 1 cut(s) 839
Csp6I GTAC 1 cut(s) 643
CviAII CATG 4 cut(s) 111, 526, 536, 543
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 4 cut(s) 390, 624, 660, 700
DpnI GATC 3 cut(s) 195, 208, 534
DpnII GATC 3 cut(s) 193, 206, 532
EaeI YGGCCR 1 cut(s) 786
Eam1104I CTCTTC 4 cut(s) 445, 603, 765, 928
EarI CTCTTC 4 cut(s) 445, 603, 765, 928
EciI GGCGGA 2 cut(s) 114, 120
Ecl136II GAGCTC 2 cut(s) 613, 758
Eco130I CCWWGG 1 cut(s) 465
Eco24I GRGCYC 2 cut(s) 615, 760
Eco47I GGWCC 1 cut(s) 218
Eco53kI GAGCTC 2 cut(s) 613, 758
EcoICRI GAGCTC 2 cut(s) 613, 758
EcoRII CCWGG 2 cut(s) 322, 858
EcoT14I CCWWGG 1 cut(s) 465
EcoT22I ATGCAT 1 cut(s) 544
EcoT38I GRGCYC 2 cut(s) 615, 760
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 4 cut(s) 114, 529, 539, 546
FatI CATG 4 cut(s) 110, 525, 535, 542
FauNDI CATATG 2 cut(s) 400, 655
FbaI TGATCA 2 cut(s) 193, 532
Fnu4HI GCNGC 8 cut(s) 258, 594, 720, 742, 840, 864, 867, 870
FriOI GRGCYC 2 cut(s) 615, 760
Fsp4HI GCNGC 8 cut(s) 258, 594, 720, 742, 840, 864, 867, 870
FspBI CTAG 3 cut(s) 77, 473, 753
GluI GCNGC 8 cut(s) 258, 594, 720, 742, 840, 864, 867, 870
HaeIII GGCC 1 cut(s) 788
HapII CCGG 2 cut(s) 151, 902
HgaI GACGC 1 cut(s) 839
Hin1II CATG 4 cut(s) 114, 529, 539, 546
HinfI GANTC 4 cut(s) 82, 406, 768, 936
HpaII CCGG 2 cut(s) 151, 902
HphI GGTGA 1 cut(s) 55
Hpy188I TCNGA 3 cut(s) 157, 198, 767
Hpy188III TCNNGA 5 cut(s) 221, 362, 515, 536, 940
HpyAV CCTTC 2 cut(s) 364, 492
HpyCH4III ACNGT 4 cut(s) 176, 269, 634, 727
HpyF10VI GCNNNNNNNGC 4 cut(s) 257, 395, 839, 869
HpyF3I CTNAG 4 cut(s) 390, 624, 660, 700
Hsp92II CATG 4 cut(s) 114, 529, 539, 546
Ksp22I TGATCA 2 cut(s) 193, 532
Kzo9I GATC 3 cut(s) 193, 206, 532
LguI GCTCTTC 2 cut(s) 603, 765
LmnI GCTCC 1 cut(s) 327
Lsp1109I GCAGC 7 cut(s) 269, 605, 731, 850, 851, 856, 878
LweI GCATC 2 cut(s) 529, 791
MaeI CTAG 3 cut(s) 77, 473, 753
MaeIII GTNAC 1 cut(s) 43
MalI GATC 3 cut(s) 195, 208, 534
MboI GATC 3 cut(s) 193, 206, 532
MboII GAAGA 5 cut(s) 211, 462, 620, 752, 915
MfeI CAATTG 2 cut(s) 65, 252
MhlI GDGCHC 2 cut(s) 615, 760
MluCI AATT 3 cut(s) 65, 252, 317
MlyI GAGTC 1 cut(s) 415
MmeI TCCRAC 1 cut(s) 135
Mph1103I ATGCAT 1 cut(s) 544
MseI TTAA 5 cut(s) 167, 300, 504, 734, 932
MspA1I CMGCKG 1 cut(s) 869
MspI CCGG 2 cut(s) 151, 902
MspR9I CCNGG 3 cut(s) 324, 860, 902
MunI CAATTG 2 cut(s) 65, 252
MvaI CCWGG 2 cut(s) 324, 860
MvnI CGCG 1 cut(s) 929
MwoI GCNNNNNNNGC 4 cut(s) 257, 395, 839, 869
NciI CCSGG 1 cut(s) 902
NdeI CATATG 2 cut(s) 400, 655
NdeII GATC 3 cut(s) 193, 206, 532
NlaIII CATG 4 cut(s) 114, 529, 539, 546
NlaIV GGNNCC 1 cut(s) 178
NmuCI GTSAC 1 cut(s) 43
NsiI ATGCAT 1 cut(s) 544
PagI TCATGA 1 cut(s) 535
PaqCI CACCTGC 1 cut(s) 235
PciSI GCTCTTC 2 cut(s) 603, 765
PfeI GAWTC 3 cut(s) 82, 768, 936
PkrI GCNGC 8 cut(s) 259, 595, 721, 743, 841, 865, 868, 871
PleI GAGTC 1 cut(s) 414
PpsI GAGTC 1 cut(s) 414
Psp124BI GAGCTC 2 cut(s) 615, 760
Psp6I CCWGG 2 cut(s) 322, 858
PspGI CCWGG 2 cut(s) 322, 858
PspN4I GGNNCC 1 cut(s) 178
PspPI GGNCC 1 cut(s) 218
PstI CTGCAG 2 cut(s) 868, 874
PvuII CAGCTG 1 cut(s) 869
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
SacI GAGCTC 2 cut(s) 615, 760
SapI GCTCTTC 2 cut(s) 603, 765
SaqAI TTAA 5 cut(s) 167, 300, 504, 734, 932
SatI GCNGC 8 cut(s) 258, 594, 720, 742, 840, 864, 867, 870
Sau3AI GATC 3 cut(s) 193, 206, 532
Sau96I GGNCC 1 cut(s) 218
SchI GAGTC 1 cut(s) 415
ScrFI CCNGG 3 cut(s) 324, 860, 902
SduI GDGCHC 2 cut(s) 615, 760
SfaNI GCATC 2 cut(s) 529, 791
SfcI CTRYAG 2 cut(s) 864, 870
SinI GGWCC 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 3 cut(s) 65, 252, 317
SsiI CCGC 4 cut(s) 99, 105, 741, 927
SspMI CTAG 3 cut(s) 77, 473, 753
SstI GAGCTC 2 cut(s) 615, 760
StyD4I CCNGG 3 cut(s) 322, 858, 900
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 4 cut(s) 176, 269, 634, 727
TaqI TCGA 3 cut(s) 90, 182, 429
TasI AATT 3 cut(s) 65, 252, 317
TauI GCSGC 1 cut(s) 744
TfiI GAWTC 3 cut(s) 82, 768, 936
Tru1I TTAA 5 cut(s) 167, 300, 504, 734, 932
Tru9I TTAA 5 cut(s) 167, 300, 504, 734, 932
TseFI GTSAC 1 cut(s) 43
TseI GCWGC 7 cut(s) 257, 593, 719, 839, 863, 866, 869
Tsp45I GTSAC 1 cut(s) 43
TspDTI ATGAA 4 cut(s) 240, 514, 621, 842
VpaK11BI GGWCC 1 cut(s) 218
XapI RAATTY 1 cut(s) 317
XspI CTAG 3 cut(s) 77, 473, 753
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.