MD12G1237600.v1.1

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0007788
Physical Location & Seq
Reverse (-)
31122566 .. 31126033
3468 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1237600.v1.1.491

Sequence Viewer

Length: 909 bp
ATGGTGAGCAAAACGGAAGAAACCCAATTGAACAGGCTCGAGAACCAGGTCGATAATGGAGGAGGAGGGGCCTGGGAGTACCTCTGCTTGGTCCGAAAGCTCAAGGTCCGCCGCTCCGAGAAGGTCTTGAAGTACGGCCTCTCGATCTTGAATGACCTCAAAAAAAGATCCAGTCTGGGCACGGAAGAGTGGACTTTATATGAGCAAGTGGCAATTGCAGCCATGGACTGCCAGTCTCTTAATGTTGCAAAGGACTGCGTTAGGCTTTTACGTAAGGCATTTCCAGAAAGTAAAAGAGTTGGTAGGCTAGAGGCTATGTTGCTAGAGGCAAAGGGATCTTGGGCAGAGGCAGAAAAGGCTTACTCAAGCTTTTTGGAGGAAAATCCACTCGATCAAGTGATACATAAGAGAAGAGTAGCTATGGCAAAGGCACAAGGAGACACTTCAGGGGCCATTGAGTGGCTTAATAAATATCTGGAAATATTTATGGCTGATCATGATGCATGGAGAGAGCTGGCTGAAATCTATGTTTCCTTGCAAATGTACAAGCAAGCTGCGTTCTGCTACGAGGAGCTGATATTATCGCAACCTACTGTTCCTCTTCACCACTTAGCTTATGCTGATGTGCTTTACACTCTTGGTGGATTGGAAAACCTTCAGACCGCAAAAAAATACTATGCATCCGTTATAGACTTGACTGGGGGCAAGAATACCAGAGCACTTTTTGGTATTAACTTGTGCACTTCTGCCATCGGACAGCTTGCAAAGGGAAGGAACAAGGAAGACAAAGAGAGCTTAGAGCTACAATCGCTGGCAGCAACAGCGTTGGAGAAAGACTACAAGCAGAGAGCTCCTGACAAACTTTCCCTGCTTACGACCGCTTTGAAAAGCTTGAAAGTTTCATCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19

Protein Analysis

303

Amino Acids

33.91

Weight (kDa)

8.42

Isoelectric Point (pI)

44.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_EMC2 PF22890 100 - 209 7e-58 EMC2 TPR-like repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 459
AccBSI CCGCTC 1 cut(s) 114
AciI CCGC 4 cut(s) 109, 112, 663, 879
AclWI GGATC 2 cut(s) 162, 343
AcuI CTGAAG 2 cut(s) 429, 641
AfaI GTAC 3 cut(s) 80, 134, 545
AfiI CCNNNNNNNGG 2 cut(s) 88, 459
AgsI TTSAA 5 cut(s) 31, 130, 151, 886, 895
AhdI GACNNNNNGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 45, 71
Alw21I GWGCWC 3 cut(s) 721, 743, 853
Alw26I GTCTC 2 cut(s) 240, 432
Alw44I GTGCAC 1 cut(s) 739
AlwI GGATC 2 cut(s) 162, 343
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 3 cut(s) 69, 136, 450
ApaLI GTGCAC 1 cut(s) 739
ApeKI GCWGC 3 cut(s) 218, 554, 815
Asp700I GAANNNNTTC 1 cut(s) 654
AspS9I GGNCC 4 cut(s) 69, 91, 106, 450
AsuHPI GGTGA 2 cut(s) 16, 596
AvaI CYCGRG 1 cut(s) 38
AvaII GGWCC 2 cut(s) 91, 106
BaeGI GKGCMC 2 cut(s) 182, 743
BanII GRGCYC 1 cut(s) 853
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 3 cut(s) 721, 743, 853
BbvI GCAGC 3 cut(s) 230, 541, 827
BccI CCATC 1 cut(s) 758
BceAI ACGGC 1 cut(s) 151
BciT130I CCWGG 2 cut(s) 47, 73
BclI TGATCA 1 cut(s) 493
BcoDI GTCTC 2 cut(s) 240, 432
BfaI CTAG 2 cut(s) 308, 323
BisI GCNGC 4 cut(s) 112, 219, 555, 816
BlsI GCNGC 4 cut(s) 113, 220, 556, 817
Bme1390I CCNGG 2 cut(s) 47, 73
Bme18I GGWCC 2 cut(s) 91, 106
BmeRI GACNNNNNGTC 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 4 cut(s) 69, 91, 106, 450
BmiI GGNNCC 2 cut(s) 70, 451
BmrFI CCNGG 2 cut(s) 47, 73
BmrI ACTGGG 1 cut(s) 708
BmsI GCATC 2 cut(s) 490, 689
BmuI ACTGGG 1 cut(s) 708
BpiI GAAGAC 1 cut(s) 789
BpuEI CTTGAG 2 cut(s) 86, 349
BsaAI YACGTR 1 cut(s) 272
BsaJI CCNNGG 2 cut(s) 72, 222
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 459
Bse1I ACTGG 3 cut(s) 171, 232, 703
BseBI CCWGG 2 cut(s) 47, 73
BseDI CCNNGG 2 cut(s) 72, 222
BseGI GGATG 1 cut(s) 680
BseLI CCNNNNNNNGG 2 cut(s) 88, 459
BseNI ACTGG 3 cut(s) 171, 232, 703
BseRI GAGGAG 3 cut(s) 75, 78, 584
BseSI GKGCMC 2 cut(s) 182, 743
BseXI GCAGC 3 cut(s) 230, 541, 827
Bsh1285I CGRYCG 1 cut(s) 879
BshFI GGCC 3 cut(s) 71, 138, 452
BsiEI CGRYCG 1 cut(s) 879
BsiHKAI GWGCWC 3 cut(s) 721, 743, 853
BsiHKCI CYCGRG 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 88, 459
BsmAI GTCTC 2 cut(s) 240, 432
BsnI GGCC 3 cut(s) 71, 138, 452
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 4 cut(s) 182, 721, 743, 853
Bsp1407I TGTACA 1 cut(s) 543
Bsp143I GATC 5 cut(s) 144, 167, 335, 391, 493
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 4 cut(s) 109, 112, 663, 879
BspANI GGCC 3 cut(s) 71, 138, 452
BspHI TCATGA 1 cut(s) 496
BspLI GGNNCC 2 cut(s) 70, 451
BspPI GGATC 2 cut(s) 162, 343
BsrBI CCGCTC 1 cut(s) 114
BsrGI TGTACA 1 cut(s) 543
BsrI ACTGG 3 cut(s) 171, 232, 703
BssECI CCNNGG 2 cut(s) 72, 222
BssMI GATC 5 cut(s) 144, 167, 335, 391, 493
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 2 cut(s) 47, 73
Bst4CI ACNGT 1 cut(s) 595
Bst6I CTCTTC 3 cut(s) 180, 406, 606
BstAUI TGTACA 1 cut(s) 543
BstBAI YACGTR 1 cut(s) 272
BstC8I GCNNGC 4 cut(s) 516, 552, 762, 813
BstDEI CTNAG 2 cut(s) 610, 796
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 680
BstKTI GATC 5 cut(s) 147, 170, 338, 394, 496
BstMAI GTCTC 2 cut(s) 240, 432
BstMBI GATC 5 cut(s) 144, 167, 335, 391, 493
BstMCI CGRYCG 1 cut(s) 879
BstMWI GCNNNNNNNGC 4 cut(s) 218, 356, 808, 821
BstNI CCWGG 2 cut(s) 47, 73
BstSCI CCNGG 2 cut(s) 45, 71
BstSLI GKGCMC 2 cut(s) 182, 743
BstSNI TACGTA 1 cut(s) 272
BstV1I GCAGC 3 cut(s) 230, 541, 827
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 2 cut(s) 167, 335
BstYI RGATCY 2 cut(s) 167, 335
BsuRI GGCC 3 cut(s) 71, 138, 452
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 680
Cac8I GCNNGC 4 cut(s) 516, 552, 762, 813
CciI TCATGA 1 cut(s) 496
Cfr13I GGNCC 4 cut(s) 69, 91, 106, 450
CsiI ACCWGGT 1 cut(s) 45
Csp6I GTAC 3 cut(s) 79, 133, 544
CviAII CATG 3 cut(s) 223, 497, 504
CviQI GTAC 3 cut(s) 79, 133, 544
DdeI CTNAG 2 cut(s) 610, 796
DpnI GATC 5 cut(s) 146, 169, 337, 393, 495
DpnII GATC 5 cut(s) 144, 167, 335, 391, 493
DriI GACNNNNNGTC 1 cut(s) 232
Eam1104I CTCTTC 3 cut(s) 180, 406, 606
Eam1105I GACNNNNNGTC 1 cut(s) 232
EarI CTCTTC 3 cut(s) 180, 406, 606
EciI GGCGGA 1 cut(s) 98
Ecl136II GAGCTC 1 cut(s) 851
Eco105I TACGTA 1 cut(s) 272
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 1 cut(s) 853
Eco47I GGWCC 2 cut(s) 91, 106
Eco53kI GAGCTC 1 cut(s) 851
Eco57I CTGAAG 2 cut(s) 429, 641
Eco88I CYCGRG 1 cut(s) 38
EcoICRI GAGCTC 1 cut(s) 851
EcoO109I RGGNCCY 1 cut(s) 69
EcoRII CCWGG 2 cut(s) 45, 71
EcoT14I CCWWGG 1 cut(s) 222
EcoT22I ATGCAT 2 cut(s) 505, 682
EcoT38I GRGCYC 1 cut(s) 853
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 3 cut(s) 226, 500, 507
FatI CATG 3 cut(s) 222, 496, 503
FbaI TGATCA 1 cut(s) 493
Fnu4HI GCNGC 4 cut(s) 112, 219, 555, 816
FokI GGATG 1 cut(s) 667
FriOI GRGCYC 1 cut(s) 853
Fsp4HI GCNGC 4 cut(s) 112, 219, 555, 816
FspBI CTAG 2 cut(s) 308, 323
GluI GCNGC 4 cut(s) 112, 219, 555, 816
HaeIII GGCC 3 cut(s) 71, 138, 452
Hin1II CATG 3 cut(s) 226, 500, 507
HindIII AAGCTT 2 cut(s) 367, 889
HphI GGTGA 2 cut(s) 16, 596
Hpy166II GTNNAC 2 cut(s) 192, 741
Hpy188I TCNGA 4 cut(s) 95, 118, 660, 755
Hpy188III TCNNGA 8 cut(s) 40, 127, 142, 148, 284, 476, 497, 854
Hpy8I GTNNAC 2 cut(s) 192, 741
HpyAV CCTTC 3 cut(s) 115, 665, 765
HpyCH4III ACNGT 1 cut(s) 595
HpyCH4IV ACGT 1 cut(s) 271
HpyCH4V TGCA 7 cut(s) 218, 248, 503, 538, 680, 741, 764
HpyF10VI GCNNNNNNNGC 4 cut(s) 218, 356, 808, 821
HpyF3I CTNAG 2 cut(s) 610, 796
HpySE526I ACGT 1 cut(s) 271
Hsp92II CATG 3 cut(s) 226, 500, 507
Ksp22I TGATCA 1 cut(s) 493
Kzo9I GATC 5 cut(s) 144, 167, 335, 391, 493
LmnI GCTCC 3 cut(s) 119, 571, 856
Lsp1109I GCAGC 3 cut(s) 230, 541, 827
LweI GCATC 2 cut(s) 490, 689
MabI ACCWGGT 1 cut(s) 45
MaeI CTAG 2 cut(s) 308, 323
MaeII ACGT 1 cut(s) 271
MalI GATC 5 cut(s) 146, 169, 337, 393, 495
MbiI CCGCTC 1 cut(s) 114
MboI GATC 5 cut(s) 144, 167, 335, 391, 493
MboII GAAGA 5 cut(s) 29, 197, 423, 593, 794
MfeI CAATTG 2 cut(s) 26, 213
MflI RGATCY 2 cut(s) 167, 335
MhlI GDGCHC 4 cut(s) 182, 721, 743, 853
MluCI AATT 2 cut(s) 26, 213
MmeI TCCRAC 1 cut(s) 807
Mph1103I ATGCAT 2 cut(s) 505, 682
MroXI GAANNNNTTC 1 cut(s) 654
MseI TTAA 3 cut(s) 240, 465, 732
MspR9I CCNGG 2 cut(s) 47, 73
MunI CAATTG 2 cut(s) 26, 213
MvaI CCWGG 2 cut(s) 47, 73
MwoI GCNNNNNNNGC 4 cut(s) 218, 356, 808, 821
NcoI CCATGG 1 cut(s) 222
NdeII GATC 5 cut(s) 144, 167, 335, 391, 493
NlaIII CATG 3 cut(s) 226, 500, 507
NlaIV GGNNCC 2 cut(s) 70, 451
NsiI ATGCAT 2 cut(s) 505, 682
PaeR7I CTCGAG 1 cut(s) 38
PagI TCATGA 1 cut(s) 496
PdmI GAANNNNTTC 1 cut(s) 654
PflMI CCANNNNNTGG 1 cut(s) 459
PkrI GCNGC 4 cut(s) 113, 220, 556, 817
Ppu21I YACGTR 1 cut(s) 272
Psp124BI GAGCTC 1 cut(s) 853
Psp6I CCWGG 2 cut(s) 45, 71
PspGI CCWGG 2 cut(s) 45, 71
PspN4I GGNNCC 2 cut(s) 70, 451
PspPI GGNCC 4 cut(s) 69, 91, 106, 450
PsuI RGATCY 2 cut(s) 167, 335
RsaI GTAC 3 cut(s) 80, 134, 545
RsaNI GTAC 3 cut(s) 79, 133, 544
SacI GAGCTC 1 cut(s) 853
SaqAI TTAA 3 cut(s) 240, 465, 732
SatI GCNGC 4 cut(s) 112, 219, 555, 816
Sau3AI GATC 5 cut(s) 144, 167, 335, 391, 493
Sau96I GGNCC 4 cut(s) 69, 91, 106, 450
ScrFI CCNGG 2 cut(s) 47, 73
SduI GDGCHC 4 cut(s) 182, 721, 743, 853
SexAI ACCWGGT 1 cut(s) 45
SfaNI GCATC 2 cut(s) 490, 689
Sfr274I CTCGAG 1 cut(s) 38
SinI GGWCC 2 cut(s) 91, 106
SlaI CTCGAG 1 cut(s) 38
SmlI CTYRAG 3 cut(s) 38, 101, 364
SmoI CTYRAG 3 cut(s) 38, 101, 364
SnaBI TACGTA 1 cut(s) 272
Sse9I AATT 2 cut(s) 26, 213
SsiI CCGC 4 cut(s) 109, 112, 663, 879
SspI AATATT 1 cut(s) 483
SspMI CTAG 2 cut(s) 308, 323
SstI GAGCTC 1 cut(s) 853
StyD4I CCNGG 2 cut(s) 45, 71
StyI CCWWGG 1 cut(s) 222
TaaI ACNGT 1 cut(s) 595
TaiI ACGT 1 cut(s) 274
TaqI TCGA 4 cut(s) 39, 51, 143, 390
TasI AATT 2 cut(s) 26, 213
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 114
Tru1I TTAA 3 cut(s) 240, 465, 732
Tru9I TTAA 3 cut(s) 240, 465, 732
TseI GCWGC 3 cut(s) 218, 554, 815
TspDTI ATGAA 1 cut(s) 891
TspGWI ACGGA 3 cut(s) 29, 197, 673
Van91I CCANNNNNTGG 1 cut(s) 459
VneI GTGCAC 1 cut(s) 739
VpaK11BI GGWCC 2 cut(s) 91, 106
XcmI CCANNNNNNNNNTGG 1 cut(s) 53
XhoI CTCGAG 1 cut(s) 38
XmnI GAANNNNTTC 1 cut(s) 654
XspI CTAG 2 cut(s) 308, 323
Zsp2I ATGCAT 2 cut(s) 505, 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.