RLG00000015364

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0007788
Physical Location & Seq
Forward (+)
65047021 .. 65050561
3541 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015364

Sequence Viewer

Length: 564 bp
ATGGTTATTGGTTTCCAAGAATGGACTCTATATGAGCAGGTTGCACTTGCAGCCATGGACTGCCAGTGTCTTGATGTTACAAAGGCATTTCCAGAGAGTAAAAGAGTTGGCAAGCTAGAGGCTATGTTGCTTGAGGCAAAGGGGTCCTGGGCAGAGGCAGAGAAGGCTTATCTAATATGTAATACACAACAGAAGGGTAGATATGGCAAAGGCTCAAGGCGATACTCAGGGGTCATTGAATGGCTTAATAAATATCTTGAAATATTTATGGCCGATAATGATGCTTGGAGAGAGTTGGCAAAAATCTATGTCTCGTTGCAAATGTATCAAGCTGCGTTCTGCTATGAGGAGCTGATATTAGCACAACCTACTATTCTTTACACTCTTAGTGGATTAGAAAACGTACAGACAGCAAAGAAATACTACGCATCTGTTATAGACTTGACTGGGGGCAAGAATATTGGAGCACTATTTGGTATTAACTTGTGTACTTCTGCCGTTGCACAGCTTGCGAAAGGAAGGAACAAGGAAGACAAGGAGACCCCGGAGCAATGTTGTAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_16 TPR_19

Protein Analysis

188

Amino Acids

21.0

Weight (kDa)

5.67

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_EMC2 PF22890 26 - 132 8.2e-29 EMC2 TPR-like repeat domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 28
AcoI YGGCCR 1 cut(s) 270
AfaI GTAC 2 cut(s) 405, 490
AgsI TTSAA 2 cut(s) 239, 260
AjnI CCWGG 1 cut(s) 146
AluBI AGCT 4 cut(s) 115, 332, 352, 508
AluI AGCT 4 cut(s) 115, 332, 352, 508
Alw21I GWGCWC 1 cut(s) 469
Alw26I GTCTC 2 cut(s) 316, 533
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 2 cut(s) 50, 332
AspS9I GGNCC 1 cut(s) 144
AsuC2I CCSGG 1 cut(s) 545
AvaII GGWCC 1 cut(s) 144
BbsI GAAGAC 1 cut(s) 537
Bbv12I GWGCWC 1 cut(s) 469
BbvI GCAGC 2 cut(s) 62, 319
BceAI ACGGC 1 cut(s) 482
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 148
BcnI CCSGG 1 cut(s) 545
BcoDI GTCTC 2 cut(s) 316, 533
BfaI CTAG 1 cut(s) 116
BfuAI ACCTGC 1 cut(s) 28
BisI GCNGC 2 cut(s) 51, 333
BlsI GCNGC 2 cut(s) 52, 334
Bme1390I CCNGG 2 cut(s) 148, 545
Bme18I GGWCC 1 cut(s) 144
BmgT120I GGNCC 1 cut(s) 144
BmiI GGNNCC 1 cut(s) 145
BmrFI CCNGG 2 cut(s) 148, 545
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 2 cut(s) 271, 437
BmuI ACTGGG 1 cut(s) 456
BpiI GAAGAC 1 cut(s) 537
BpuEI CTTGAG 2 cut(s) 152, 199
BpuMI CCSGG 1 cut(s) 545
BsaI GGTCTC 1 cut(s) 533
BsaJI CCNNGG 3 cut(s) 54, 147, 543
Bse1I ACTGG 2 cut(s) 64, 451
Bse3DI GCAATG 1 cut(s) 557
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 3 cut(s) 54, 147, 543
BseMI GCAATG 1 cut(s) 557
BseMII CTCAG 1 cut(s) 240
BseNI ACTGG 2 cut(s) 64, 451
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 2 cut(s) 62, 319
BshFI GGCC 1 cut(s) 272
BsiHKAI GWGCWC 1 cut(s) 469
BsiSI CCGG 1 cut(s) 545
BsmAI GTCTC 2 cut(s) 316, 533
BsnI GGCC 1 cut(s) 272
Bso31I GGTCTC 1 cut(s) 533
Bsp1286I GDGCHC 1 cut(s) 469
Bsp19I CCATGG 1 cut(s) 54
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 1 cut(s) 239
BspLI GGNNCC 1 cut(s) 145
BspMI ACCTGC 1 cut(s) 28
BspTNI GGTCTC 1 cut(s) 533
BsrDI GCAATG 1 cut(s) 557
BsrI ACTGG 2 cut(s) 64, 451
BssECI CCNNGG 3 cut(s) 54, 147, 543
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 148
BstAPI GCANNNNNTGC 1 cut(s) 509
BstC8I GCNNGC 2 cut(s) 113, 510
BstDEI CTNAG 2 cut(s) 226, 386
BstDSI CCRYGG 1 cut(s) 54
BstMAI GTCTC 2 cut(s) 316, 533
BstMWI GCNNNNNNNGC 3 cut(s) 50, 164, 509
BstNI CCWGG 1 cut(s) 148
BstSCI CCNGG 2 cut(s) 146, 543
BstV1I GCAGC 2 cut(s) 62, 319
BstV2I GAAGAC 1 cut(s) 537
BsuRI GGCC 1 cut(s) 272
BtgI CCRYGG 1 cut(s) 54
BtsIMutI CAGTG 1 cut(s) 71
BveI ACCTGC 1 cut(s) 28
Cac8I GCNNGC 2 cut(s) 113, 510
Cfr13I GGNCC 1 cut(s) 144
Csp6I GTAC 2 cut(s) 404, 489
CviAII CATG 1 cut(s) 55
CviQI GTAC 2 cut(s) 404, 489
DdeI CTNAG 2 cut(s) 226, 386
EaeI YGGCCR 1 cut(s) 270
Eco130I CCWWGG 1 cut(s) 54
Eco31I GGTCTC 1 cut(s) 533
Eco47I GGWCC 1 cut(s) 144
EcoO109I RGGNCCY 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 1 cut(s) 58
FatI CATG 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 51, 333
Fsp4HI GCNGC 2 cut(s) 51, 333
FspBI CTAG 1 cut(s) 116
GluI GCNGC 2 cut(s) 51, 333
HaeIII GGCC 1 cut(s) 272
HapII CCGG 1 cut(s) 545
Hin1II CATG 1 cut(s) 58
HinfI GANTC 1 cut(s) 25
HpaII CCGG 1 cut(s) 545
Hpy166II GTNNAC 1 cut(s) 489
Hpy188III TCNNGA 3 cut(s) 71, 92, 257
Hpy8I GTNNAC 1 cut(s) 489
HpyAV CCTTC 3 cut(s) 157, 187, 513
HpyCH4IV ACGT 1 cut(s) 402
HpyCH4V TGCA 4 cut(s) 44, 50, 319, 503
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 164, 509
HpyF3I CTNAG 2 cut(s) 226, 386
HpySE526I ACGT 1 cut(s) 402
Hsp92II CATG 1 cut(s) 58
LmnI GCTCC 3 cut(s) 349, 464, 547
LpnPI CCDG 8 cut(s) 23, 77, 105, 133, 160, 213, 432, 558
Lsp1109I GCAGC 2 cut(s) 62, 319
LweI GCATC 2 cut(s) 271, 437
MaeI CTAG 1 cut(s) 116
MaeII ACGT 1 cut(s) 402
MaeIII GTNAC 1 cut(s) 76
MboII GAAGA 1 cut(s) 542
MhlI GDGCHC 1 cut(s) 469
MlyI GAGTC 1 cut(s) 19
MnlI CCTC 4 cut(s) 112, 127, 148, 340
MseI TTAA 2 cut(s) 246, 480
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 2 cut(s) 148, 545
MvaI CCWGG 1 cut(s) 148
MwoI GCNNNNNNNGC 3 cut(s) 50, 164, 509
NciI CCSGG 1 cut(s) 545
NcoI CCATGG 1 cut(s) 54
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 145
PkrI GCNGC 2 cut(s) 52, 334
PleI GAGTC 1 cut(s) 19
PpsI GAGTC 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 144
Psp5II RGGWCCY 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 146
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 145
PspPI GGNCC 1 cut(s) 144
PspPPI RGGWCCY 1 cut(s) 144
RsaI GTAC 2 cut(s) 405, 490
RsaNI GTAC 2 cut(s) 404, 489
SaqAI TTAA 2 cut(s) 246, 480
SatI GCNGC 2 cut(s) 51, 333
Sau96I GGNCC 1 cut(s) 144
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 2 cut(s) 148, 545
SduI GDGCHC 1 cut(s) 469
SetI ASST 7 cut(s) 42, 117, 334, 354, 370, 405, 510
SfaNI GCATC 2 cut(s) 271, 437
SinI GGWCC 1 cut(s) 144
SmlI CTYRAG 2 cut(s) 131, 214
SmoI CTYRAG 2 cut(s) 131, 214
SspI AATATT 2 cut(s) 264, 460
SspMI CTAG 1 cut(s) 116
StyD4I CCNGG 2 cut(s) 146, 543
StyI CCWWGG 1 cut(s) 54
TaiI ACGT 1 cut(s) 405
TatI WGTACW 1 cut(s) 488
Tru1I TTAA 2 cut(s) 246, 480
Tru9I TTAA 2 cut(s) 246, 480
TscAI CASTG 1 cut(s) 71
TseI GCWGC 2 cut(s) 50, 332
TspRI CASTG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 144
XspI CTAG 1 cut(s) 116
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.