AT3G05545

zinc finger (C3HC4 type RING finger) family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005970
Physical Location & Seq
Forward (+)
1609050 .. 1612607
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05545.1

Sequence Viewer

Length: 1278 bp
ATGGGTTTAGGCAATAAGGGTAACAAATTCAATTTCGGCGATAATGATCTCGCCGACGAAGCTAGTGGTGCCGATGGCGACGAAGGTGATGGTTTTGGTTCGGTTGCGTGTTCGATTTGTCTCGAGACGGTTGTTAAAAACGGCGATCGAGCATGGGCTAATCTTCAATGTGACCATCAGTTCCATCTAGATTGTATTGGTTCGGCTTTCAATGCAAAGGGAGTGATGCAATGCCCCAACTGTCGGAAAGTAGAAAAAGGCCAGTGGCTTTATGCAAACGGTTGTCGTTCATATCCCGAATTCAATGTCGAGGATTGGGTTCACGAAGAAGATATTTATGATATTGGAGCATACTCTGAAATGTCTTTTGGAGTTCACTGGTGCCCATTTGGAAGCTCAGCCCGTCTTCCTTCTTTCGAGGATGGAGAATTTTCACCGAGTTCATATCACGATCTTTTGGGACAGCAAGGTTACTATACTGAACCAGCAGCGCCAACCGCAGGTCATCCATGTCCATATGTAACCTATTTTGGCCCGGTTCACTCGCCTTCCTCAAGCAGTGGTGGTGCTGCAGGCGTTTCAGACAGTTCAAGTTTCAGTAGTCACTGGAACACTGGCTCTTCGGTTTCTGGTGAAGTCCCAACTCCTTATGGTTTCCCTGTGGATCCGCACTATCACGGCTGGGATTATCATCCGCCTCCACCCCCACCGCCACAGCATTTCTCTGCTTCTGGCGCTCATGTGGGCAGTCCAACTCAGCCCACGCCTCCACCAGCCGCTGCGAGGACTTCTCGAGCCAACGGCTCAGATATGATCAGACCCAGACCGCCACATTTCACGCGTCCATTTCATGGTCACAGTTCCAGTGGTAGAGCGGGTAGCTCGGTGGCATCTGTTCCGAGGACACCACCATTTCCAGGAAGCAACGCTCGGACCCGAGACAGAATGCAGGCTCTTCAAGCCTATTACCAACAGTCTTCTGCACAGTCACACCAGCCGGATTCGCCTATAGTCTCTCGAGGGCCTGTATTCCCATCTGGCCGGAGGCCCGCTAGAGGAATAGCTTCAGGGATGGGATCAACGTCATCTTCTTCAGATCAGGCAGGTGGGAGTGGTTTTATCCGGTTTAACATATGGGAGAGAGATCCTTATATGCAATCACAACAAGCCTACTCGGTTAATCAGATGGACAGAGAACCAAACATTTGGACATCTTCATTTAATGAAGGATCAGGAAGCTTCCATCAGAGGCACGGTGGCGGAGGAGGATCATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_11 zf-RING_2

Protein Analysis

425

Amino Acids

45.51

Weight (kDa)

5.91

Isoelectric Point (pI)

57.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 37 - 82 3.5e-07 Ring finger domain
zf-RING_11 PF17123 37 - 66 2.9e-06 RING-like zinc finger
zf-C3HC4 PF00097 37 - 81 7.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1094
Acc36I ACCTGC 2 cut(s) 491, 1094
AccB1I GGYRCC 2 cut(s) 68, 381
AccB7I CCANNNNNTGG 1 cut(s) 851
AccBSI CCGCTC 1 cut(s) 875
AccII CGCG 1 cut(s) 841
AciI CCGC 9 cut(s) 498, 668, 695, 710, 777, 827, 875, 1050, 1260
AclWI GGATC 6 cut(s) 659, 672, 1084, 1139, 1237, 1276
AcoI YGGCCR 1 cut(s) 1039
AcsI RAATTY 3 cut(s) 26, 299, 428
AcuI CTGAAG 2 cut(s) 1050, 1077
AfiI CCNNNNNNNGG 6 cut(s) 243, 500, 783, 851, 917, 1055
AflIII ACRYGT 1 cut(s) 839
AgsI TTSAA 6 cut(s) 31, 167, 211, 304, 591, 959
AjnI CCWGG 1 cut(s) 916
AjuI GAANNNNNNNTTGG 2 cut(s) 351, 383
AluBI AGCT 5 cut(s) 62, 396, 882, 1064, 1239
AluI AGCT 5 cut(s) 62, 396, 882, 1064, 1239
Alw26I GTCTC 4 cut(s) 119, 125, 933, 1018
AlwI GGATC 6 cut(s) 659, 672, 1084, 1139, 1237, 1276
AlwNI CAGNNNCTG 1 cut(s) 779
Ama87I CYCGRG 4 cut(s) 122, 792, 936, 1017
AoxI GGCC 5 cut(s) 259, 532, 1022, 1039, 1046
ApeKI GCWGC 3 cut(s) 488, 569, 779
ApoI RAATTY 3 cut(s) 26, 299, 428
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
Asp700I GAANNNNTTC 1 cut(s) 1063
AspLEI GCGC 2 cut(s) 493, 737
AspS9I GGNCC 4 cut(s) 533, 933, 1022, 1047
AsuC2I CCSGG 1 cut(s) 536
AsuHPI GGTGA 3 cut(s) 98, 426, 644
AvaI CYCGRG 4 cut(s) 122, 792, 936, 1017
AvaII GGWCC 1 cut(s) 933
BaeGI GKGCMC 1 cut(s) 386
BamHI GGATCC 1 cut(s) 664
BanI GGYRCC 2 cut(s) 68, 381
BbsI GAAGAC 2 cut(s) 398, 969
BbvI GCAGC 3 cut(s) 500, 556, 766
BccI CCATC 9 cut(s) 68, 83, 183, 192, 416, 1042, 1066, 1180, 1251
BceAI ACGGC 3 cut(s) 157, 694, 817
BciT130I CCWGG 1 cut(s) 918
BclI TGATCA 1 cut(s) 813
BcnI CCSGG 1 cut(s) 536
BcoDI GTCTC 4 cut(s) 119, 125, 933, 1018
BfaI CTAG 3 cut(s) 63, 188, 1053
BfmI CTRYAG 2 cut(s) 570, 1008
BfoI RGCGCY 2 cut(s) 494, 738
BfuAI ACCTGC 2 cut(s) 491, 1094
BisI GCNGC 4 cut(s) 489, 570, 777, 780
BlpI GCTNAGC 1 cut(s) 397
BlsI GCNGC 4 cut(s) 490, 571, 778, 781
Bme1390I CCNGG 2 cut(s) 536, 918
Bme18I GGWCC 1 cut(s) 933
BmeT110I CYCGRG 4 cut(s) 122, 792, 936, 1017
BmgT120I GGNCC 4 cut(s) 533, 933, 1022, 1047
BmiI GGNNCC 4 cut(s) 70, 383, 666, 935
BmrFI CCNGG 2 cut(s) 536, 918
BmsI GCATC 2 cut(s) 216, 899
BpiI GAAGAC 2 cut(s) 398, 969
BplI GAGNNNNNCTC 2 cut(s) 775, 807
Bpu1102I GCTNAGC 1 cut(s) 397
BpuEI CTTGAG 1 cut(s) 538
BpuMI CCSGG 1 cut(s) 536
BsaBI GATNNNNATC 2 cut(s) 45, 690
BsaJI CCNNGG 1 cut(s) 899
BsaWI WCCGGW 1 cut(s) 1122
Bsc4I CCNNNNNNNGG 6 cut(s) 243, 500, 783, 851, 917, 1055
Bse1I ACTGG 5 cut(s) 262, 383, 611, 619, 864
Bse3DI GCAATG 1 cut(s) 236
Bse8I GATNNNNATC 2 cut(s) 45, 690
BseBI CCWGG 1 cut(s) 918
BseDI CCNNGG 1 cut(s) 899
BseGI GGATG 4 cut(s) 427, 505, 691, 1077
BseJI GATNNNNATC 2 cut(s) 45, 690
BseLI CCNNNNNNNGG 6 cut(s) 243, 500, 783, 851, 917, 1055
BseMI GCAATG 1 cut(s) 236
BseMII CTCAG 3 cut(s) 411, 770, 819
BseNI ACTGG 5 cut(s) 262, 383, 611, 619, 864
BseRI GAGGAG 1 cut(s) 1278
BseSI GKGCMC 1 cut(s) 386
BseXI GCAGC 3 cut(s) 500, 556, 766
BseYI CCCAGC 1 cut(s) 681
BsgI GTGCAG 1 cut(s) 966
Bsh1236I CGCG 1 cut(s) 841
Bsh1285I CGRYCG 1 cut(s) 148
BshFI GGCC 5 cut(s) 261, 534, 1024, 1041, 1048
BshNI GGYRCC 2 cut(s) 68, 381
BsiEI CGRYCG 1 cut(s) 148
BsiHKCI CYCGRG 4 cut(s) 122, 792, 936, 1017
BsiSI CCGG 4 cut(s) 536, 998, 1042, 1123
BslFI GGGAC 2 cut(s) 474, 623
BslI CCNNNNNNNGG 6 cut(s) 243, 500, 783, 851, 917, 1055
BsmAI GTCTC 4 cut(s) 119, 125, 933, 1018
BsmBI CGTCTC 1 cut(s) 119
BsmFI GGGAC 2 cut(s) 474, 623
BsmI GAATGC 1 cut(s) 951
BsnI GGCC 5 cut(s) 261, 534, 1024, 1041, 1048
BsoBI CYCGRG 4 cut(s) 122, 792, 936, 1017
Bsp1286I GDGCHC 1 cut(s) 386
Bsp1720I GCTNAGC 1 cut(s) 397
BspACI CCGC 9 cut(s) 498, 668, 695, 710, 777, 827, 875, 1050, 1260
BspANI GGCC 5 cut(s) 261, 534, 1024, 1041, 1048
BspCNI CTCAG 3 cut(s) 410, 769, 818
BspFNI CGCG 1 cut(s) 841
BspLI GGNNCC 4 cut(s) 70, 383, 666, 935
BspMAI CTGCAG 1 cut(s) 574
BspMI ACCTGC 2 cut(s) 491, 1094
BspPI GGATC 6 cut(s) 659, 672, 1084, 1139, 1237, 1276
BspQI GCTCTTC 2 cut(s) 625, 960
BspT107I GGYRCC 2 cut(s) 68, 381
BsrBI CCGCTC 1 cut(s) 875
BsrDI GCAATG 1 cut(s) 236
BsrI ACTGG 5 cut(s) 262, 383, 611, 619, 864
BssECI CCNNGG 1 cut(s) 899
Bst2UI CCWGG 1 cut(s) 918
Bst4CI ACNGT 8 cut(s) 130, 242, 281, 587, 860, 975, 987, 1256
Bst6I CTCTTC 2 cut(s) 625, 960
BstC8I GCNNGC 3 cut(s) 574, 951, 1050
BstDEI CTNAG 3 cut(s) 397, 756, 805
BstF5I GGATG 4 cut(s) 427, 505, 691, 1077
BstFNI CGCG 1 cut(s) 841
BstH2I RGCGCY 2 cut(s) 494, 738
BstHHI GCGC 2 cut(s) 493, 737
BstMAI GTCTC 4 cut(s) 119, 125, 933, 1018
BstMCI CGRYCG 1 cut(s) 148
BstMWI GCNNNNNNNGC 7 cut(s) 59, 68, 212, 497, 734, 959, 1003
BstNI CCWGG 1 cut(s) 918
BstSCI CCNGG 2 cut(s) 534, 916
BstSFI CTRYAG 2 cut(s) 570, 1008
BstSLI GKGCMC 1 cut(s) 386
BstUI CGCG 1 cut(s) 841
BstV1I GCAGC 3 cut(s) 500, 556, 766
BstV2I GAAGAC 2 cut(s) 398, 969
BstX2I RGATCY 2 cut(s) 664, 1144
BstXI CCANNNNNNTGG 1 cut(s) 1206
BstYI RGATCY 2 cut(s) 664, 1144
BsuRI GGCC 5 cut(s) 261, 534, 1024, 1041, 1048
BtsCI GGATG 4 cut(s) 427, 505, 691, 1077
BtsI GCAGTG 1 cut(s) 565
BtsIMutI CAGTG 6 cut(s) 269, 376, 565, 604, 612, 871
BveI ACCTGC 2 cut(s) 491, 1094
Cac8I GCNNGC 3 cut(s) 574, 951, 1050
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 2 cut(s) 493, 737
Cfr13I GGNCC 4 cut(s) 533, 933, 1022, 1047
CseI GACGC 1 cut(s) 830
CviAII CATG 4 cut(s) 153, 510, 740, 851
DdeI CTNAG 3 cut(s) 397, 756, 805
EaeI YGGCCR 1 cut(s) 1039
Eam1104I CTCTTC 2 cut(s) 625, 960
EarI CTCTTC 2 cut(s) 625, 960
EciI GGCGGA 2 cut(s) 684, 1275
Eco47I GGWCC 1 cut(s) 933
Eco57I CTGAAG 2 cut(s) 1050, 1077
Eco88I CYCGRG 4 cut(s) 122, 792, 936, 1017
EcoO109I RGGNCCY 1 cut(s) 1022
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 916
Esp3I CGTCTC 1 cut(s) 119
FaeI CATG 4 cut(s) 156, 513, 743, 854
FaqI GGGAC 2 cut(s) 474, 623
FatI CATG 4 cut(s) 152, 509, 739, 850
FauI CCCGC 2 cut(s) 868, 1057
FauNDI CATATG 2 cut(s) 517, 1133
FbaI TGATCA 1 cut(s) 813
Fnu4HI GCNGC 4 cut(s) 489, 570, 777, 780
FokI GGATG 4 cut(s) 434, 492, 678, 1084
Fsp4HI GCNGC 4 cut(s) 489, 570, 777, 780
FspBI CTAG 3 cut(s) 63, 188, 1053
GlaI GCGC 2 cut(s) 492, 736
GluI GCNGC 4 cut(s) 489, 570, 777, 780
GsaI CCCAGC 1 cut(s) 685
HaeII RGCGCY 2 cut(s) 494, 738
HaeIII GGCC 5 cut(s) 261, 534, 1024, 1041, 1048
HapII CCGG 4 cut(s) 536, 998, 1042, 1123
HgaI GACGC 1 cut(s) 830
HhaI GCGC 2 cut(s) 493, 737
Hin1II CATG 4 cut(s) 156, 513, 743, 854
Hin6I GCGC 2 cut(s) 491, 735
HinP1I GCGC 2 cut(s) 491, 735
HindIII AAGCTT 1 cut(s) 1237
HinfI GANTC 1 cut(s) 1001
HpaII CCGG 4 cut(s) 536, 998, 1042, 1123
HphI GGTGA 3 cut(s) 98, 426, 644
Hpy166II GTNNAC 3 cut(s) 322, 376, 541
Hpy188III TCNNGA 9 cut(s) 122, 124, 188, 296, 323, 449, 792, 1017, 1233
Hpy8I GTNNAC 3 cut(s) 322, 376, 541
Hpy99I CGWCG 2 cut(s) 59, 83
HpyAV CCTTC 4 cut(s) 77, 420, 558, 1220
HpyCH4III ACNGT 8 cut(s) 130, 242, 281, 587, 860, 975, 987, 1256
HpyCH4IV ACGT 1 cut(s) 1082
HpyCH4V TGCA 7 cut(s) 215, 229, 275, 572, 949, 983, 1156
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 68, 212, 497, 734, 959, 1003
HpyF3I CTNAG 3 cut(s) 397, 756, 805
HpySE526I ACGT 1 cut(s) 1082
Hsp92II CATG 4 cut(s) 156, 513, 743, 854
HspAI GCGC 2 cut(s) 491, 735
Ksp22I TGATCA 1 cut(s) 813
LguI GCTCTTC 2 cut(s) 625, 960
LmnI GCTCC 1 cut(s) 347
Lsp1109I GCAGC 3 cut(s) 500, 556, 766
LweI GCATC 2 cut(s) 216, 899
MaeI CTAG 3 cut(s) 63, 188, 1053
MaeII ACGT 1 cut(s) 1082
MaeIII GTNAC 7 cut(s) 20, 170, 470, 520, 602, 854, 987
MbiI CCGCTC 1 cut(s) 875
MflI RGATCY 2 cut(s) 664, 1144
MhlI GDGCHC 1 cut(s) 386
MluCI AATT 4 cut(s) 26, 31, 299, 428
MluI ACGCGT 1 cut(s) 839
MmeI TCCRAC 2 cut(s) 224, 776
MroXI GAANNNNTTC 1 cut(s) 1063
MseI TTAA 5 cut(s) 135, 1128, 1179, 1221, 1276
MspA1I CMGCKG 1 cut(s) 779
MspI CCGG 4 cut(s) 536, 998, 1042, 1123
MspR9I CCNGG 2 cut(s) 536, 918
Mva1269I GAATGC 1 cut(s) 951
MvaI CCWGG 1 cut(s) 918
MvnI CGCG 1 cut(s) 841
MwoI GCNNNNNNNGC 7 cut(s) 59, 68, 212, 497, 734, 959, 1003
NciI CCSGG 1 cut(s) 536
NdeI CATATG 2 cut(s) 517, 1133
NlaIII CATG 4 cut(s) 156, 513, 743, 854
NlaIV GGNNCC 4 cut(s) 70, 383, 666, 935
NmuCI GTSAC 4 cut(s) 170, 602, 854, 987
PaeR7I CTCGAG 3 cut(s) 122, 792, 1017
PaqCI CACCTGC 1 cut(s) 1094
PciSI GCTCTTC 2 cut(s) 625, 960
PctI GAATGC 1 cut(s) 951
PdmI GAANNNNTTC 1 cut(s) 1063
PfeI GAWTC 1 cut(s) 1001
PflMI CCANNNNNTGG 1 cut(s) 851
PfoI TCCNGGA 1 cut(s) 916
PkrI GCNGC 4 cut(s) 490, 571, 778, 781
Ple19I CGATCG 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 916
PspFI CCCAGC 1 cut(s) 681
PspGI CCWGG 1 cut(s) 916
PspN4I GGNNCC 4 cut(s) 70, 383, 666, 935
PspPI GGNCC 4 cut(s) 533, 933, 1022, 1047
PstI CTGCAG 1 cut(s) 574
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 2 cut(s) 664, 1144
PvuI CGATCG 1 cut(s) 148
SapI GCTCTTC 2 cut(s) 625, 960
SaqAI TTAA 5 cut(s) 135, 1128, 1179, 1221, 1276
SatI GCNGC 4 cut(s) 489, 570, 777, 780
Sau96I GGNCC 4 cut(s) 533, 933, 1022, 1047
ScrFI CCNGG 2 cut(s) 536, 918
SduI GDGCHC 1 cut(s) 386
SfaNI GCATC 2 cut(s) 216, 899
SfcI CTRYAG 2 cut(s) 570, 1008
Sfr274I CTCGAG 3 cut(s) 122, 792, 1017
SinI GGWCC 1 cut(s) 933
SlaI CTCGAG 3 cut(s) 122, 792, 1017
SmlI CTYRAG 4 cut(s) 122, 553, 792, 1017
SmoI CTYRAG 4 cut(s) 122, 553, 792, 1017
Sse9I AATT 4 cut(s) 26, 31, 299, 428
SsiI CCGC 9 cut(s) 498, 668, 695, 710, 777, 827, 875, 1050, 1260
SspMI CTAG 3 cut(s) 63, 188, 1053
StyD4I CCNGG 2 cut(s) 534, 916
TaaI ACNGT 8 cut(s) 130, 242, 281, 587, 860, 975, 987, 1256
TaiI ACGT 1 cut(s) 1085
TaqI TCGA 7 cut(s) 113, 123, 148, 309, 417, 793, 1018
TasI AATT 4 cut(s) 26, 31, 299, 428
TauI GCSGC 1 cut(s) 779
TfiI GAWTC 1 cut(s) 1001
Tru1I TTAA 5 cut(s) 135, 1128, 1179, 1221, 1276
Tru9I TTAA 5 cut(s) 135, 1128, 1179, 1221, 1276
TscAI CASTG 6 cut(s) 269, 383, 565, 611, 619, 871
TseFI GTSAC 4 cut(s) 170, 602, 854, 987
TseI GCWGC 3 cut(s) 488, 569, 779
Tsp45I GTSAC 4 cut(s) 170, 602, 854, 987
TspDTI ATGAA 5 cut(s) 279, 432, 839, 1206, 1239
TspRI CASTG 6 cut(s) 269, 383, 565, 611, 619, 871
Van91I CCANNNNNTGG 1 cut(s) 851
VpaK11BI GGWCC 1 cut(s) 933
XapI RAATTY 3 cut(s) 26, 299, 428
XbaI TCTAGA 1 cut(s) 187
XhoI CTCGAG 3 cut(s) 122, 792, 1017
XmnI GAANNNNTTC 1 cut(s) 1063
XspI CTAG 3 cut(s) 63, 188, 1053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.