AT3G06035

GPI-anchored protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0008438
Physical Location & Seq
Reverse (-)
1822904 .. 1824309
1406 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G06035.1

Sequence Viewer

Length: 603 bp
ATGGCGATCGACAAGCTTCCACTTCTTCTCTTTCTCTCCATACTCCTCTGCCTCAATCGCCCTGTTCTTAGCGACACCGATGAAGAGGACATTCTTCTCACAGGAATCAACAGCTACAGAACAACACAGAATCTAACAATCTTAAGCAAGAACGAAAACGCAGAATGCCTAGCTGACGAAATCGCAGACCAATTCAAGAACAAACCTTGTACCAACGACACTGGCTCAGCCACAGTACCTGGAACTGAGCCACAGTTCGCAAACTACCCTCAGATTCTAGCAAAATGCCACTTGAACGTCTCAGACACGAGAGACGGCTCGATTATGCCTGCATGTGTTCCTCGTTTAGAATCAAACCTCGTCCTCACAAACTTCACCAAGTCACAATACTCAATGAGTTTGAACGATTCCAAGTTTACAGGGATTGGTATTGGTAAAGAAGATGATTGGATCGTTGTAGTCCTCACTACCAACACACCAGAAGGAAGCTATTCTACAGCTACTCCTACAAAACAAGAGTCTAATGGCTTCACCTTTGGTATTGGTCTCGTTAGTTATTTAGTCATTTTCATGTACTCTTCCTTTTGTTTCTTTCTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.05

Weight (kDa)

4.49

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 29 - 155 1.4e-58 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 458
AfaI GTAC 3 cut(s) 211, 237, 575
AflII CTTAAG 1 cut(s) 142
AgsI TTSAA 3 cut(s) 196, 295, 403
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 5 cut(s) 16, 114, 173, 489, 500
AluI AGCT 5 cut(s) 16, 114, 173, 489, 500
Alw26I GTCTC 3 cut(s) 304, 306, 551
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 239
ArsI GACNNNNNNTTYG 2 cut(s) 503, 535
Asp700I GAANNNNTTC 1 cut(s) 490
AsuHPI GGTGA 2 cut(s) 367, 523
BarI GAAGNNNNNNTAC 2 cut(s) 478, 510
BauI CACGAG 1 cut(s) 307
BceAI ACGGC 1 cut(s) 331
BciT130I CCWGG 1 cut(s) 240
BcoDI GTCTC 3 cut(s) 304, 306, 551
BfaI CTAG 2 cut(s) 170, 278
BfmI CTRYAG 2 cut(s) 115, 495
BfrI CTTAAG 1 cut(s) 142
BlpI GCTNAGC 1 cut(s) 226
Bme1390I CCNGG 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 240
Bpu1102I GCTNAGC 1 cut(s) 226
BsaI GGTCTC 1 cut(s) 551
BsaXI ACNNNNNCTCC 2 cut(s) 487, 517
Bse1I ACTGG 1 cut(s) 226
BseBI CCWGG 1 cut(s) 240
BseMII CTCAG 4 cut(s) 237, 240, 284, 315
BseNI ACTGG 1 cut(s) 226
BseRI GAGGAG 1 cut(s) 35
Bsh1285I CGRYCG 1 cut(s) 9
BsiEI CGRYCG 1 cut(s) 9
BsmAI GTCTC 3 cut(s) 304, 306, 551
BsmBI CGTCTC 2 cut(s) 304, 306
BsmI GAATGC 1 cut(s) 170
Bso31I GGTCTC 1 cut(s) 551
Bsp143I GATC 2 cut(s) 6, 450
Bsp1720I GCTNAGC 1 cut(s) 226
BspCNI CTCAG 4 cut(s) 238, 239, 283, 314
BspPI GGATC 1 cut(s) 458
BspTI CTTAAG 1 cut(s) 142
BspTNI GGTCTC 1 cut(s) 551
BsrI ACTGG 1 cut(s) 226
BssMI GATC 2 cut(s) 6, 450
BssSI CACGAG 1 cut(s) 307
Bst2BI CACGAG 1 cut(s) 307
Bst2UI CCWGG 1 cut(s) 240
Bst4CI ACNGT 2 cut(s) 235, 255
Bst6I CTCTTC 2 cut(s) 78, 583
BstAFI CTTAAG 1 cut(s) 142
BstC8I GCNNGC 1 cut(s) 330
BstDEI CTNAG 5 cut(s) 68, 226, 246, 270, 301
BstKTI GATC 2 cut(s) 9, 453
BstMAI GTCTC 3 cut(s) 304, 306, 551
BstMBI GATC 2 cut(s) 6, 450
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 57
BstNI CCWGG 1 cut(s) 240
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 1 cut(s) 238
BstSFI CTRYAG 2 cut(s) 115, 495
BtsIMutI CAGTG 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 330
CaiI CAGNNNCTG 1 cut(s) 239
Csp6I GTAC 3 cut(s) 210, 236, 574
CviAII CATG 2 cut(s) 333, 571
CviQI GTAC 3 cut(s) 210, 236, 574
DdeI CTNAG 5 cut(s) 68, 226, 246, 270, 301
DpnI GATC 2 cut(s) 8, 452
DpnII GATC 2 cut(s) 6, 450
Eam1104I CTCTTC 2 cut(s) 78, 583
EarI CTCTTC 2 cut(s) 78, 583
Eco31I GGTCTC 1 cut(s) 551
EcoRII CCWGG 1 cut(s) 238
Esp3I CGTCTC 2 cut(s) 304, 306
FaeI CATG 2 cut(s) 336, 574
FaiI YATR 4 cut(s) 41, 326, 334, 572
FatI CATG 2 cut(s) 332, 570
FspBI CTAG 2 cut(s) 170, 278
Hin1II CATG 2 cut(s) 336, 574
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 6 cut(s) 105, 130, 274, 350, 407, 518
HphI GGTGA 2 cut(s) 367, 523
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 2 cut(s) 273, 304
Hpy188III TCNNGA 1 cut(s) 196
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 1 cut(s) 476
HpyCH4III ACNGT 2 cut(s) 235, 255
HpyCH4IV ACGT 1 cut(s) 297
HpyCH4V TGCA 1 cut(s) 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 57
HpyF3I CTNAG 5 cut(s) 68, 226, 246, 270, 301
HpySE526I ACGT 1 cut(s) 297
Hsp92II CATG 2 cut(s) 336, 574
Kzo9I GATC 2 cut(s) 6, 450
LpnPI CCDG 8 cut(s) 75, 87, 207, 225, 252, 342, 405, 492
MaeI CTAG 2 cut(s) 170, 278
MaeII ACGT 1 cut(s) 297
MaeIII GTNAC 1 cut(s) 381
MalI GATC 2 cut(s) 8, 452
MboI GATC 2 cut(s) 6, 450
MboII GAAGA 5 cut(s) 17, 86, 95, 452, 570
MluCI AATT 1 cut(s) 191
MlyI GAGTC 1 cut(s) 527
MnlI CCTC 8 cut(s) 56, 62, 79, 279, 351, 368, 374, 473
MroXI GAANNNNTTC 1 cut(s) 490
MseI TTAA 1 cut(s) 143
MslI CAYNNNNRTG 1 cut(s) 569
MspCI CTTAAG 1 cut(s) 142
MspR9I CCNGG 1 cut(s) 240
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 1 cut(s) 57
NdeII GATC 2 cut(s) 6, 450
NlaIII CATG 2 cut(s) 336, 574
NmuCI GTSAC 1 cut(s) 381
NspI RCATGY 1 cut(s) 336
PctI GAATGC 1 cut(s) 170
PdmI GAANNNNTTC 1 cut(s) 490
PfeI GAWTC 5 cut(s) 105, 130, 274, 350, 407
Ple19I CGATCG 1 cut(s) 9
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PstNI CAGNNNCTG 1 cut(s) 239
PvuI CGATCG 1 cut(s) 9
RsaI GTAC 3 cut(s) 211, 237, 575
RsaNI GTAC 3 cut(s) 210, 236, 574
RseI CAYNNNNRTG 1 cut(s) 569
SaqAI TTAA 1 cut(s) 143
Sau3AI GATC 2 cut(s) 6, 450
SchI GAGTC 1 cut(s) 527
ScrFI CCNGG 1 cut(s) 240
SfcI CTRYAG 2 cut(s) 115, 495
SmiMI CAYNNNNRTG 1 cut(s) 569
SmlI CTYRAG 1 cut(s) 142
SmoI CTYRAG 1 cut(s) 142
Sse9I AATT 1 cut(s) 191
SspMI CTAG 2 cut(s) 170, 278
StyD4I CCNGG 1 cut(s) 238
TaaI ACNGT 2 cut(s) 235, 255
TaiI ACGT 1 cut(s) 300
TaqI TCGA 2 cut(s) 9, 320
TasI AATT 1 cut(s) 191
TatI WGTACW 1 cut(s) 573
TfiI GAWTC 5 cut(s) 105, 130, 274, 350, 407
Tru1I TTAA 1 cut(s) 143
Tru9I TTAA 1 cut(s) 143
TscAI CASTG 1 cut(s) 226
TseFI GTSAC 1 cut(s) 381
Tsp45I GTSAC 1 cut(s) 381
TspDTI ATGAA 2 cut(s) 96, 559
TspRI CASTG 1 cut(s) 226
Vha464I CTTAAG 1 cut(s) 142
XceI RCATGY 1 cut(s) 336
XmnI GAANNNNTTC 1 cut(s) 490
XspI CTAG 2 cut(s) 170, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.