Rh4AG210300

GPI-anchored protein At3g06035-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0008438
Physical Location & Seq
Reverse (-)
50344098 .. 50346717
2620 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG210300.1

Sequence Viewer

Length: 603 bp
ATGGCGTCTTCTCTTCGTCTTCTCTTTCCATTGTTCCTGTCTTGCATTGTTTTGTTGAATCACCAAGTCAAATGCGATGACGTTGATGAGGAGGATAATCTTCTTCAGGGCATCAACAGCTATAGGGCTTCTGCCAACTTAACAGCCCTTACAAAGAATGACAATGCAGAGTGCCTGGCAGATGAGATAGCTGACCAATTCAAAAAGCAACCCTGTACAAACACCACAGGTTCCTTCACGGTACCAGGCACCGAAACCCAGTTGACCAACTTTCCAGACCTTTTAGCCAAATGTCATTTGAATGTCTCAAACACAAGGGATGGAGTCATAATGCCTTCTTGCGTTCCGAATCTGGATCCTAGTCTCGTCCTTACCAACTTCACTAAGTCTCAGTACTCGTCAAATCTTAATCAGACAAATTTTACAGGAATTGGAATTGGTTCTGAAGATAACTGGATTGTTGTAATTTTGACCACGAGCACACCCGAAGGAAGCTATGTTCCTGCACAAAGCACTGACAGTGCCAGTTTAGGTTCCAAATCCAAGATTAGTTTGATTCAATACTTGCTGTGTTTGCTGATGGGGTTTGTCTTTCATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

21.76

Weight (kDa)

4.66

Isoelectric Point (pI)

33.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 31 - 157 1.6e-60 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 241
AccB1I GGYRCC 2 cut(s) 241, 248
AclWI GGATC 2 cut(s) 350, 363
AcsI RAATTY 1 cut(s) 418
AcuI CTGAAG 2 cut(s) 89, 465
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 217, 243, 395
AgsI TTSAA 4 cut(s) 58, 202, 301, 560
AjnI CCWGG 2 cut(s) 174, 244
AloI GAACNNNNNNTCC 2 cut(s) 483, 515
AluBI AGCT 3 cut(s) 120, 191, 495
AluI AGCT 3 cut(s) 120, 191, 495
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 3 cut(s) 310, 368, 393
AlwI GGATC 2 cut(s) 350, 363
ApoI RAATTY 1 cut(s) 418
Asp700I GAANNNNTTC 1 cut(s) 439
Asp718I GGTACC 1 cut(s) 241
AsuHPI GGTGA 1 cut(s) 53
BamHI GGATCC 1 cut(s) 355
BanI GGYRCC 2 cut(s) 241, 248
BauI CACGAG 1 cut(s) 475
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 482
BccI CCATC 2 cut(s) 314, 574
BciT130I CCWGG 2 cut(s) 176, 246
BcoDI GTCTC 3 cut(s) 310, 368, 393
BfaI CTAG 1 cut(s) 360
BfmI CTRYAG 1 cut(s) 121
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 2 cut(s) 176, 246
BmiI GGNNCC 5 cut(s) 232, 243, 250, 357, 535
BmrFI CCNGG 2 cut(s) 176, 246
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 1 cut(s) 120
BmuI ACTGGG 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 5
Bse1I ACTGG 3 cut(s) 259, 458, 525
BseBI CCWGG 2 cut(s) 176, 246
BseGI GGATG 1 cut(s) 325
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 3 cut(s) 259, 458, 525
BseRI GAGGAG 1 cut(s) 104
BsgI GTGCAG 1 cut(s) 489
BshNI GGYRCC 2 cut(s) 241, 248
BsiHKAI GWGCWC 1 cut(s) 482
BsmAI GTCTC 3 cut(s) 310, 368, 393
Bsp1286I GDGCHC 1 cut(s) 482
Bsp1407I TGTACA 1 cut(s) 215
Bsp143I GATC 1 cut(s) 355
BspCNI CTCAG 1 cut(s) 403
BspLI GGNNCC 5 cut(s) 232, 243, 250, 357, 535
BspPI GGATC 2 cut(s) 350, 363
BspT107I GGYRCC 2 cut(s) 241, 248
BsrGI TGTACA 1 cut(s) 215
BsrI ACTGG 3 cut(s) 259, 458, 525
BssMI GATC 1 cut(s) 355
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 475
Bst2UI CCWGG 2 cut(s) 176, 246
Bst4CI ACNGT 2 cut(s) 241, 521
Bst6I CTCTTC 1 cut(s) 18
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 215
BstDEI CTNAG 2 cut(s) 384, 390
BstF5I GGATG 1 cut(s) 325
BstKTI GATC 1 cut(s) 358
BstMAI GTCTC 3 cut(s) 310, 368, 393
BstMBI GATC 1 cut(s) 355
BstMWI GCNNNNNNNGC 2 cut(s) 117, 574
BstNI CCWGG 2 cut(s) 176, 246
BstSCI CCNGG 2 cut(s) 174, 244
BstSFI CTRYAG 1 cut(s) 121
BstV2I GAAGAC 1 cut(s) 11
BstX2I RGATCY 1 cut(s) 355
BstYI RGATCY 1 cut(s) 355
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 513, 526
Csp6I GTAC 3 cut(s) 216, 242, 394
CviJI RGCY 6 cut(s) 120, 128, 146, 191, 287, 495
CviKI_1 RGCY 6 cut(s) 120, 128, 146, 191, 287, 495
CviQI GTAC 3 cut(s) 216, 242, 394
DdeI CTNAG 2 cut(s) 384, 390
DpnI GATC 1 cut(s) 357
DpnII GATC 1 cut(s) 355
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 89, 465
EcoRII CCWGG 2 cut(s) 174, 244
FaiI YATR 3 cut(s) 123, 329, 498
FokI GGATG 1 cut(s) 332
FspBI CTAG 1 cut(s) 360
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 264
HindII GTYRAC 1 cut(s) 264
HinfI GANTC 4 cut(s) 58, 324, 349, 556
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 3 cut(s) 348, 414, 445
Hpy188III TCNNGA 2 cut(s) 275, 353
Hpy8I GTNNAC 1 cut(s) 264
HpyAV CCTTC 3 cut(s) 244, 345, 482
HpyCH4III ACNGT 2 cut(s) 241, 521
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 3 cut(s) 45, 167, 506
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 574
HpyF3I CTNAG 2 cut(s) 384, 390
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 5
KpnI GGTACC 1 cut(s) 245
Kzo9I GATC 1 cut(s) 355
LweI GCATC 1 cut(s) 120
MaeI CTAG 1 cut(s) 360
MaeII ACGT 1 cut(s) 81
MalI GATC 1 cut(s) 357
MboI GATC 1 cut(s) 355
MboII GAAGA 5 cut(s) 5, 11, 92, 95, 458
MflI RGATCY 1 cut(s) 355
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 5 cut(s) 197, 418, 429, 435, 465
MlyI GAGTC 1 cut(s) 333
MnlI CCTC 2 cut(s) 82, 85
MroXI GAANNNNTTC 1 cut(s) 439
MseI TTAA 2 cut(s) 140, 408
MslI CAYNNNNRTG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 176, 246
MvaI CCWGG 2 cut(s) 176, 246
MwoI GCNNNNNNNGC 2 cut(s) 117, 574
NdeII GATC 1 cut(s) 355
NlaIV GGNNCC 5 cut(s) 232, 243, 250, 357, 535
PdmI GAANNNNTTC 1 cut(s) 439
PfeI GAWTC 3 cut(s) 58, 349, 556
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Psp6I CCWGG 2 cut(s) 174, 244
PspGI CCWGG 2 cut(s) 174, 244
PspN4I GGNNCC 5 cut(s) 232, 243, 250, 357, 535
PsuI RGATCY 1 cut(s) 355
RsaI GTAC 3 cut(s) 217, 243, 395
RsaNI GTAC 3 cut(s) 216, 242, 394
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 2 cut(s) 140, 408
Sau3AI GATC 1 cut(s) 355
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 2 cut(s) 176, 246
SduI GDGCHC 1 cut(s) 482
SetI ASST 7 cut(s) 84, 122, 193, 232, 282, 497, 535
SfaNI GCATC 1 cut(s) 120
SfcI CTRYAG 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 5 cut(s) 197, 418, 429, 435, 465
SspMI CTAG 1 cut(s) 360
StyD4I CCNGG 2 cut(s) 174, 244
TaaI ACNGT 2 cut(s) 241, 521
TaiI ACGT 1 cut(s) 84
TasI AATT 5 cut(s) 197, 418, 429, 435, 465
TatI WGTACW 2 cut(s) 215, 393
TfiI GAWTC 3 cut(s) 58, 349, 556
Tru1I TTAA 2 cut(s) 140, 408
Tru9I TTAA 2 cut(s) 140, 408
TscAI CASTG 2 cut(s) 520, 526
TspDTI ATGAA 1 cut(s) 584
TspRI CASTG 2 cut(s) 520, 526
XapI RAATTY 1 cut(s) 418
XmnI GAANNNNTTC 1 cut(s) 439
XspI CTAG 1 cut(s) 360
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.