Rorug04G0146800

GPI-anchored protein At3g06035-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0008438
Physical Location & Seq
Forward (+)
25479792 .. 25482409
2618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0146800.1

Sequence Viewer

Length: 603 bp
ATGAAAATGGGGATTGAACGAGTGCTTAAAGAAAAGTCTGGAGATGCACTCAAGGATATTCAACAAGTCTTTGATGAAGAAAACAGAATGACCACAGTTGAGGTTCTCGTCCTAGATGGGGTGATTCAGCTGACAGAAAGGGATGAATGTGTCTATCAAGAAATCATTACTCATCTTCCTCTCTGCCCAATTCCAAACCCCAAGAAGGTTTTGGTTATCGGTGGAGGAGATGGTGGTGTACTGAGGGAAGTGGCTAGTCATTCTTCTATCAAGCAAATAGATATATGTGAGATTGATAAGATGGTTTCAAAACAGTTTTTCTCTCGTGTAGCTATTGGATATGAGGATCCCAGGGTGACACTCCATGTTGGTGATGGAGTCGCATTTTCAAAGGCTGTTCTAGCAGGAACCTATGATGCAATAATTGTGGACTCTTCTGATCCCATAGGTAATACTGTTTCATTTAACAGCTGCTGCAGTGAAAAGAGTTTGAGTTTGAAGAAGCGGAAGAAGAAGGAGAAGTTCCAAATGCAGCTTGCCAAGAAAGATGACATCTGGGCAGAATCGGCAGTAGGTCTTATGCAAAAGAATTTCAGTCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

200

Amino Acids

22.13

Weight (kDa)

5.98

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Spermine_synth PF01564 49 - 155 8.4e-39 Spermine/spermidine synthase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 505
AclWI GGATC 3 cut(s) 341, 354, 434
AcsI RAATTY 1 cut(s) 589
AfaI GTAC 1 cut(s) 240
AfiI CCNNNNNNNGG 2 cut(s) 118, 205
AgsI TTSAA 5 cut(s) 17, 62, 309, 390, 499
AjnI CCWGG 1 cut(s) 350
AluBI AGCT 4 cut(s) 130, 332, 471, 535
AluI AGCT 4 cut(s) 130, 332, 471, 535
AlwI GGATC 3 cut(s) 341, 354, 434
AlwNI CAGNNNCTG 1 cut(s) 474
ApeKI GCWGC 3 cut(s) 471, 474, 532
ApoI RAATTY 1 cut(s) 589
AsuHPI GGTGA 3 cut(s) 133, 367, 383
BamHI GGATCC 1 cut(s) 346
BauI CACGAG 1 cut(s) 324
BbvI GCAGC 3 cut(s) 458, 461, 544
BccI CCATC 4 cut(s) 110, 224, 295, 368
BciT130I CCWGG 1 cut(s) 352
BfaI CTAG 3 cut(s) 113, 255, 401
BfmI CTRYAG 1 cut(s) 475
BisI GCNGC 3 cut(s) 472, 475, 533
BlsI GCNGC 3 cut(s) 473, 476, 534
Bme1390I CCNGG 1 cut(s) 352
BmiI GGNNCC 2 cut(s) 348, 409
BmrFI CCNGG 1 cut(s) 352
BmsI GCATC 2 cut(s) 34, 406
BplI GAGNNNNNCTC 2 cut(s) 33, 65
BpmI CTGGAG 1 cut(s) 60
BpuEI CTTGAG 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 350, 351
Bsc4I CCNNNNNNNGG 2 cut(s) 118, 205
BseBI CCWGG 1 cut(s) 352
BseDI CCNNGG 2 cut(s) 350, 351
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 2 cut(s) 118, 205
BseMII CTCAG 1 cut(s) 233
BseRI GAGGAG 1 cut(s) 240
BseXI GCAGC 3 cut(s) 458, 461, 544
BslI CCNNNNNNNGG 2 cut(s) 118, 205
Bsp143I GATC 2 cut(s) 346, 439
BspACI CCGC 1 cut(s) 505
BspCNI CTCAG 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 348, 409
BspMAI CTGCAG 1 cut(s) 479
BspPI GGATC 3 cut(s) 341, 354, 434
BssECI CCNNGG 2 cut(s) 350, 351
BssMI GATC 2 cut(s) 346, 439
BssSI CACGAG 1 cut(s) 324
Bst2BI CACGAG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 352
Bst4CI ACNGT 3 cut(s) 97, 315, 457
Bst6I CTCTTC 1 cut(s) 439
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 1 cut(s) 242
BstF5I GGATG 1 cut(s) 148
BstKTI GATC 2 cut(s) 349, 442
BstMBI GATC 2 cut(s) 346, 439
BstMWI GCNNNNNNNGC 2 cut(s) 401, 566
BstNI CCWGG 1 cut(s) 352
BstSCI CCNGG 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 475
BstV1I GCAGC 3 cut(s) 458, 461, 544
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BtsCI GGATG 1 cut(s) 148
BtsI GCAGTG 1 cut(s) 484
BtsIMutI CAGTG 1 cut(s) 484
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 474
Csp6I GTAC 1 cut(s) 239
CspCI CAANNNNNGTGG 2 cut(s) 408, 443
CviAII CATG 1 cut(s) 365
CviJI RGCY 6 cut(s) 130, 254, 332, 395, 471, 535
CviKI_1 RGCY 6 cut(s) 130, 254, 332, 395, 471, 535
CviQI GTAC 1 cut(s) 239
DdeI CTNAG 1 cut(s) 242
DpnI GATC 2 cut(s) 348, 441
DpnII GATC 2 cut(s) 346, 439
Eam1104I CTCTTC 1 cut(s) 439
EarI CTCTTC 1 cut(s) 439
EcoRII CCWGG 1 cut(s) 350
FaeI CATG 1 cut(s) 368
FaiI YATR 7 cut(s) 284, 286, 342, 366, 414, 446, 581
FatI CATG 1 cut(s) 364
Fnu4HI GCNGC 3 cut(s) 472, 475, 533
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 3 cut(s) 472, 475, 533
FspBI CTAG 3 cut(s) 113, 255, 401
GluI GCNGC 3 cut(s) 472, 475, 533
GsuI CTGGAG 1 cut(s) 60
Hin1II CATG 1 cut(s) 368
HinfI GANTC 4 cut(s) 124, 378, 431, 563
HphI GGTGA 3 cut(s) 133, 367, 383
Hpy166II GTNNAC 2 cut(s) 239, 430
Hpy188I TCNGA 1 cut(s) 439
Hpy188III TCNNGA 2 cut(s) 39, 158
Hpy8I GTNNAC 2 cut(s) 239, 430
HpyAV CCTTC 2 cut(s) 199, 508
HpyCH4III ACNGT 3 cut(s) 97, 315, 457
HpyCH4V TGCA 5 cut(s) 47, 419, 477, 532, 583
HpyF10VI GCNNNNNNNGC 2 cut(s) 401, 566
HpyF3I CTNAG 1 cut(s) 242
Hsp92II CATG 1 cut(s) 368
Kzo9I GATC 2 cut(s) 346, 439
LpnPI CCDG 5 cut(s) 24, 337, 364, 390, 541
Lsp1109I GCAGC 3 cut(s) 458, 461, 544
LweI GCATC 2 cut(s) 34, 406
MaeI CTAG 3 cut(s) 113, 255, 401
MaeIII GTNAC 1 cut(s) 355
MalI GATC 2 cut(s) 348, 441
MboI GATC 2 cut(s) 346, 439
MboII GAAGA 7 cut(s) 89, 167, 255, 426, 511, 520, 523
MflI RGATCY 1 cut(s) 346
MluCI AATT 3 cut(s) 189, 423, 589
MlyI GAGTC 2 cut(s) 387, 425
MnlI CCTC 5 cut(s) 94, 189, 218, 237, 337
MseI TTAA 3 cut(s) 27, 465, 601
MslI CAYNNNNRTG 1 cut(s) 369
MspA1I CMGCKG 2 cut(s) 130, 471
MspR9I CCNGG 1 cut(s) 352
MvaI CCWGG 1 cut(s) 352
MwoI GCNNNNNNNGC 2 cut(s) 401, 566
NdeII GATC 2 cut(s) 346, 439
NlaIII CATG 1 cut(s) 368
NlaIV GGNNCC 2 cut(s) 348, 409
NmuCI GTSAC 1 cut(s) 355
PasI CCCWGGG 1 cut(s) 351
PfeI GAWTC 2 cut(s) 124, 563
PkrI GCNGC 3 cut(s) 473, 476, 534
PleI GAGTC 2 cut(s) 386, 425
PpsI GAGTC 2 cut(s) 386, 425
Psp6I CCWGG 1 cut(s) 350
PspGI CCWGG 1 cut(s) 350
PspN4I GGNNCC 2 cut(s) 348, 409
PstI CTGCAG 1 cut(s) 479
PstNI CAGNNNCTG 1 cut(s) 474
PsuI RGATCY 1 cut(s) 346
PvuII CAGCTG 2 cut(s) 130, 471
RsaI GTAC 1 cut(s) 240
RsaNI GTAC 1 cut(s) 239
RseI CAYNNNNRTG 1 cut(s) 369
SaqAI TTAA 3 cut(s) 27, 465, 601
SatI GCNGC 3 cut(s) 472, 475, 533
Sau3AI GATC 2 cut(s) 346, 439
SchI GAGTC 2 cut(s) 387, 425
ScrFI CCNGG 1 cut(s) 352
SetI ASST 9 cut(s) 105, 132, 210, 334, 413, 451, 473, 537, 577
SfaNI GCATC 2 cut(s) 34, 406
SfcI CTRYAG 1 cut(s) 475
SmiMI CAYNNNNRTG 1 cut(s) 369
SmlI CTYRAG 1 cut(s) 50
SmoI CTYRAG 1 cut(s) 50
Sse9I AATT 3 cut(s) 189, 423, 589
SsiI CCGC 1 cut(s) 505
SspMI CTAG 3 cut(s) 113, 255, 401
StyD4I CCNGG 1 cut(s) 350
TaaI ACNGT 3 cut(s) 97, 315, 457
TasI AATT 3 cut(s) 189, 423, 589
TatI WGTACW 1 cut(s) 238
TfiI GAWTC 2 cut(s) 124, 563
Tru1I TTAA 3 cut(s) 27, 465, 601
Tru9I TTAA 3 cut(s) 27, 465, 601
TscAI CASTG 1 cut(s) 484
TseFI GTSAC 1 cut(s) 355
TseI GCWGC 3 cut(s) 471, 474, 532
Tsp45I GTSAC 1 cut(s) 355
TspDTI ATGAA 4 cut(s) 17, 90, 159, 450
TspRI CASTG 1 cut(s) 484
XapI RAATTY 1 cut(s) 589
XcmI CCANNNNNNNNNTGG 2 cut(s) 208, 371
XspI CTAG 3 cut(s) 113, 255, 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.