MD16G1187000.v1.1

GPI-anchored protein At3g06035-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0008438
Physical Location & Seq
Forward (+)
16159576 .. 16162022
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1187000.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCGTCTCCGCTGCGTCTCCTCCTTCTCCCACTATTACTTTCCTGCATTGTTTTGTTGAGCCACCAGGTCAAATGCGATGGAGTAGATGAAGAAGATAGTCTTCTTCAAGGCATCAACAGCTATAGGACTGCCACCTTGAACCTAACGGCCCTTACAAAGAATGCCAACGCAGAGTGCCTTGCTGATGAGATAGCTGAGCAATTCAAAAATCAACCCTGTACAAACACCACAGGCTCCAACACAGTACCAGGCACTGAACCTAATTTCCCCGACTACCCAAAACTTTTAGCCAAATGCCATTTGAATGTCTCTAACACAAGAGATGGGGTCATAATGCCTGCTTGCGTTCCCAGCCTCGTTCCTAGTCTGGTCCTTACCAACTTCACAAAGTCTCAGTACTCGGATAAACTTAACGACACCAAGTTTACCGGAATTGGAATTGGCTCTGAAGATAACTGGATTGTTGTTGTTCTGACCACCAGCACACCAGAAGGAAGCTTTGTGACTGCACAAAGTTCACCAGATGCACAAGATTCCACCGGTTCTGCTTCCAAGATCAGTTTGGTTCCCTACTTGCTATTTCTACTAATGGGTTTTATCTTTCATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

205

Amino Acids

21.97

Weight (kDa)

4.8

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 32 - 159 4.1e-60 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 11
AcuI CTGAAG 1 cut(s) 471
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 3 cut(s) 223, 249, 401
AgeI ACCGGT 1 cut(s) 542
AgsI TTSAA 4 cut(s) 110, 142, 208, 307
AjnI CCWGG 2 cut(s) 66, 250
AloI GAACNNNNNNTCC 2 cut(s) 252, 284
AluBI AGCT 3 cut(s) 123, 197, 501
AluI AGCT 3 cut(s) 123, 197, 501
Alw26I GTCTC 4 cut(s) 12, 23, 316, 399
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 1 cut(s) 150
ApeKI GCWGC 1 cut(s) 13
AsiGI ACCGGT 1 cut(s) 542
AspS9I GGNCC 2 cut(s) 151, 373
AsuHPI GGTGA 1 cut(s) 513
AvaII GGWCC 1 cut(s) 373
BbsI GAAGAC 1 cut(s) 95
BccI CCATC 2 cut(s) 74, 320
BceAI ACGGC 1 cut(s) 165
BciT130I CCWGG 2 cut(s) 68, 252
BcoDI GTCTC 4 cut(s) 12, 23, 316, 399
BfaI CTAG 1 cut(s) 366
BfmI CTRYAG 1 cut(s) 124
BisI GCNGC 1 cut(s) 14
BlpI GCTNAGC 1 cut(s) 198
BlsI GCNGC 1 cut(s) 15
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 2 cut(s) 68, 252
Bme18I GGWCC 1 cut(s) 373
BmgT120I GGNCC 2 cut(s) 151, 373
BmiI GGNNCC 2 cut(s) 238, 570
BmrFI CCNGG 2 cut(s) 68, 252
BmsI GCATC 2 cut(s) 123, 517
BpiI GAAGAC 1 cut(s) 95
Bpu1102I GCTNAGC 1 cut(s) 198
BsaHI GRCGYC 1 cut(s) 5
BsaWI WCCGGW 2 cut(s) 431, 542
Bse118I RCCGGY 1 cut(s) 542
Bse1I ACTGG 1 cut(s) 464
BseBI CCWGG 2 cut(s) 68, 252
BseMII CTCAG 2 cut(s) 189, 410
BseNI ACTGG 1 cut(s) 464
BseRI GAGGAG 1 cut(s) 11
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 495
BshFI GGCC 1 cut(s) 152
BshTI ACCGGT 1 cut(s) 542
BsiSI CCGG 2 cut(s) 432, 543
BsmAI GTCTC 4 cut(s) 12, 23, 316, 399
BsmBI CGTCTC 2 cut(s) 12, 23
BsmI GAATGC 1 cut(s) 169
BsnI GGCC 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 221
Bsp143I GATC 1 cut(s) 558
Bsp1720I GCTNAGC 1 cut(s) 198
BspACI CCGC 1 cut(s) 11
BspANI GGCC 1 cut(s) 152
BspCNI CTCAG 2 cut(s) 190, 409
BspLI GGNNCC 2 cut(s) 238, 570
BsrFI RCCGGY 1 cut(s) 542
BsrGI TGTACA 1 cut(s) 221
BsrI ACTGG 1 cut(s) 464
BssAI RCCGGY 1 cut(s) 542
BssMI GATC 1 cut(s) 558
BssNI GRCGYC 1 cut(s) 5
Bst2UI CCWGG 2 cut(s) 68, 252
Bst4CI ACNGT 1 cut(s) 247
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 342, 346
BstDEI CTNAG 2 cut(s) 198, 396
BstKTI GATC 1 cut(s) 561
BstMAI GTCTC 4 cut(s) 12, 23, 316, 399
BstMBI GATC 1 cut(s) 558
BstMWI GCNNNNNNNGC 2 cut(s) 120, 354
BstNI CCWGG 2 cut(s) 68, 252
BstSCI CCNGG 2 cut(s) 66, 250
BstSFI CTRYAG 1 cut(s) 124
BstV2I GAAGAC 1 cut(s) 95
BsuRI GGCC 1 cut(s) 152
BtgZI GCGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 342, 346
CaiI CAGNNNCTG 1 cut(s) 257
Cfr10I RCCGGY 1 cut(s) 542
Cfr13I GGNCC 2 cut(s) 151, 373
CseI GACGC 1 cut(s) 5
CsiI ACCWGGT 1 cut(s) 66
Csp6I GTAC 3 cut(s) 222, 248, 400
CspAI ACCGGT 1 cut(s) 542
CviJI RGCY 9 cut(s) 63, 123, 152, 197, 237, 293, 357, 447, 501
CviKI_1 RGCY 9 cut(s) 63, 123, 152, 197, 237, 293, 357, 447, 501
CviQI GTAC 3 cut(s) 222, 248, 400
DdeI CTNAG 2 cut(s) 198, 396
DpnI GATC 1 cut(s) 560
DpnII GATC 1 cut(s) 558
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 471
EcoRII CCWGG 2 cut(s) 66, 250
Esp3I CGTCTC 2 cut(s) 12, 23
FaiI YATR 2 cut(s) 126, 335
FalI AAGNNNNNCTT 2 cut(s) 87, 119
Fnu4HI GCNGC 1 cut(s) 14
Fsp4HI GCNGC 1 cut(s) 14
FspBI CTAG 1 cut(s) 366
GluI GCNGC 1 cut(s) 14
GsaI CCCAGC 1 cut(s) 357
HaeIII GGCC 1 cut(s) 152
HapII CCGG 2 cut(s) 432, 543
HgaI GACGC 1 cut(s) 5
Hin1I GRCGYC 1 cut(s) 5
HindIII AAGCTT 1 cut(s) 499
HinfI GANTC 1 cut(s) 536
HpaII CCGG 2 cut(s) 432, 543
HphI GGTGA 1 cut(s) 513
Hpy166II GTNNAC 2 cut(s) 429, 521
Hpy188I TCNGA 3 cut(s) 406, 451, 477
Hpy8I GTNNAC 2 cut(s) 429, 521
HpyAV CCTTC 2 cut(s) 35, 488
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4V TGCA 3 cut(s) 48, 512, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 120, 354
HpyF3I CTNAG 2 cut(s) 198, 396
Hsp92I GRCGYC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 558
LmnI GCTCC 1 cut(s) 242
LweI GCATC 2 cut(s) 123, 517
MabI ACCWGGT 1 cut(s) 66
MaeI CTAG 1 cut(s) 366
MaeIII GTNAC 1 cut(s) 505
MalI GATC 1 cut(s) 560
MboI GATC 1 cut(s) 558
MboII GAAGA 5 cut(s) 95, 98, 104, 107, 464
MluCI AATT 4 cut(s) 203, 265, 435, 441
MmeI TCCRAC 1 cut(s) 264
MnlI CCTC 2 cut(s) 32, 368
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 2 cut(s) 432, 543
MspR9I CCNGG 2 cut(s) 68, 252
Mva1269I GAATGC 1 cut(s) 169
MvaI CCWGG 2 cut(s) 68, 252
MwoI GCNNNNNNNGC 2 cut(s) 120, 354
NdeII GATC 1 cut(s) 558
NlaIV GGNNCC 2 cut(s) 238, 570
NmuCI GTSAC 1 cut(s) 505
PctI GAATGC 1 cut(s) 169
PfeI GAWTC 1 cut(s) 536
PinAI ACCGGT 1 cut(s) 542
PkrI GCNGC 1 cut(s) 15
Psp6I CCWGG 2 cut(s) 66, 250
PspFI CCCAGC 1 cut(s) 353
PspGI CCWGG 2 cut(s) 66, 250
PspN4I GGNNCC 2 cut(s) 238, 570
PspPI GGNCC 2 cut(s) 151, 373
PstNI CAGNNNCTG 1 cut(s) 257
RsaI GTAC 3 cut(s) 223, 249, 401
RsaNI GTAC 3 cut(s) 222, 248, 400
RseI CAYNNNNRTG 1 cut(s) 306
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 1 cut(s) 558
Sau96I GGNCC 2 cut(s) 151, 373
ScaI AGTACT 1 cut(s) 401
ScrFI CCNGG 2 cut(s) 68, 252
SetI ASST 7 cut(s) 72, 125, 140, 147, 199, 265, 503
SexAI ACCWGGT 1 cut(s) 66
SfaNI GCATC 2 cut(s) 123, 517
SfcI CTRYAG 1 cut(s) 124
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 306
Sse9I AATT 4 cut(s) 203, 265, 435, 441
SsiI CCGC 1 cut(s) 11
SspMI CTAG 1 cut(s) 366
StyD4I CCNGG 2 cut(s) 66, 250
TaaI ACNGT 1 cut(s) 247
TasI AATT 4 cut(s) 203, 265, 435, 441
TatI WGTACW 2 cut(s) 221, 399
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 505
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 1 cut(s) 505
TspDTI ATGAA 2 cut(s) 105, 596
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 562
XspI CTAG 1 cut(s) 366
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.