RLG00000007918

GPI-anchored protein At3g06035-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0008438
Physical Location & Seq
Forward (+)
22915650 .. 22917763
2114 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007918

Sequence Viewer

Length: 651 bp
ATGGCGTCTTCTCTTCGTCTTCTCTTTCCATTGTTCCTGTCTTGCATTGTTTTGTTGGATCACCAAGTCAAATGCGATGACGTTGATGAGGAGGATAATCTTCTTCAAGGCATCAACAGCTATAGGGCTTCTGCCAACTTAACAGCCCTTACAAAGAATGACAATGCAGAGTGCCTGGCAGATGAGATAGCTGACCAATTCAAAAAGCAACCCTGTACAAACACCACAGGTTCCTTCACAGTACCAGGCACTGAAACCCAGTTTACCAACTTTCCAGACCTTTTAGCCAAATGTCATTTGAATGTCTCAAACACAAGGGATGGAGTCATTATGCCTTCCTGCGTTCCGAATCTGGATGCAAGTCTCGTCCTTACCAACTTCACTAAGTCTCAGTACTCGTCGAATCTTAATCAGACAAATTTTACAGGAATTGGAATTGGTTCTGAAGATAACTGGATTGTTGTTATTTTGACCACGAGCACGCCCGAAGGAAGCTATGTTCCTGCACAAAGCACTGACAGTGCCACTGGCAGTGCCAGTTTAGACCAAGAGGTTTATCATACCAACTTTCTGCTTTTCCGACCTAGAGAGGACACTGATTCTTCCATTGGAGCTGCTTCCGTGGTGGAATTCGATTTCGTACTTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

23.46

Weight (kDa)

4.24

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 31 - 157 2.6e-60 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 66
AcsI RAATTY 2 cut(s) 418, 629
AcuI CTGAAG 1 cut(s) 465
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 4 cut(s) 217, 243, 395, 642
AgsI TTSAA 3 cut(s) 107, 202, 301
AjnI CCWGG 2 cut(s) 174, 244
AloI GAACNNNNNNTCC 2 cut(s) 483, 515
AluBI AGCT 4 cut(s) 120, 191, 495, 614
AluI AGCT 4 cut(s) 120, 191, 495, 614
Alw21I GWGCWC 1 cut(s) 482
Alw26I GTCTC 3 cut(s) 310, 368, 393
AlwI GGATC 1 cut(s) 66
AlwNI CAGNNNCTG 1 cut(s) 251
ApeKI GCWGC 1 cut(s) 614
ApoI RAATTY 2 cut(s) 418, 629
Asp700I GAANNNNTTC 1 cut(s) 439
AsuHPI GGTGA 1 cut(s) 53
BauI CACGAG 1 cut(s) 475
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 482
BbvI GCAGC 1 cut(s) 601
BccI CCATC 1 cut(s) 314
BciT130I CCWGG 2 cut(s) 176, 246
BcoDI GTCTC 3 cut(s) 310, 368, 393
BfaI CTAG 1 cut(s) 585
BfmI CTRYAG 1 cut(s) 121
BisI GCNGC 1 cut(s) 615
BlsI GCNGC 1 cut(s) 616
BmcAI AGTACT 1 cut(s) 395
Bme1390I CCNGG 2 cut(s) 176, 246
BmiI GGNNCC 1 cut(s) 232
BmrFI CCNGG 2 cut(s) 176, 246
BmrI ACTGGG 1 cut(s) 253
BmsI GCATC 2 cut(s) 120, 346
BmuI ACTGGG 1 cut(s) 253
BpiI GAAGAC 1 cut(s) 11
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 1 cut(s) 621
Bse1I ACTGG 4 cut(s) 259, 458, 532, 537
BseBI CCWGG 2 cut(s) 176, 246
BseDI CCNNGG 1 cut(s) 621
BseGI GGATG 2 cut(s) 325, 361
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 4 cut(s) 259, 458, 532, 537
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 1 cut(s) 601
BsgI GTGCAG 1 cut(s) 489
BsiHKAI GWGCWC 1 cut(s) 482
BsmAI GTCTC 3 cut(s) 310, 368, 393
Bsp1286I GDGCHC 1 cut(s) 482
Bsp1407I TGTACA 1 cut(s) 215
Bsp143I GATC 1 cut(s) 58
BspCNI CTCAG 1 cut(s) 403
BspLI GGNNCC 1 cut(s) 232
BspPI GGATC 1 cut(s) 66
BsrGI TGTACA 1 cut(s) 215
BsrI ACTGG 4 cut(s) 259, 458, 532, 537
BssECI CCNNGG 1 cut(s) 621
BssMI GATC 1 cut(s) 58
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 475
Bst2BI CACGAG 1 cut(s) 475
Bst2UI CCWGG 2 cut(s) 176, 246
Bst4CI ACNGT 2 cut(s) 241, 521
Bst6I CTCTTC 1 cut(s) 18
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 215
BstC8I GCNNGC 1 cut(s) 482
BstDEI CTNAG 2 cut(s) 384, 390
BstDSI CCRYGG 1 cut(s) 621
BstF5I GGATG 2 cut(s) 325, 361
BstKTI GATC 1 cut(s) 61
BstMAI GTCTC 3 cut(s) 310, 368, 393
BstMBI GATC 1 cut(s) 58
BstMWI GCNNNNNNNGC 1 cut(s) 117
BstNI CCWGG 2 cut(s) 176, 246
BstSCI CCNGG 2 cut(s) 174, 244
BstSFI CTRYAG 1 cut(s) 121
BstV1I GCAGC 1 cut(s) 601
BstV2I GAAGAC 1 cut(s) 11
BtgI CCRYGG 1 cut(s) 621
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 2 cut(s) 325, 361
BtsI GCAGTG 1 cut(s) 538
BtsIMutI CAGTG 6 cut(s) 249, 513, 525, 526, 538, 594
Cac8I GCNNGC 1 cut(s) 482
CaiI CAGNNNCTG 1 cut(s) 251
Csp6I GTAC 4 cut(s) 216, 242, 394, 641
CviJI RGCY 7 cut(s) 120, 128, 146, 191, 287, 495, 614
CviKI_1 RGCY 7 cut(s) 120, 128, 146, 191, 287, 495, 614
CviQI GTAC 4 cut(s) 216, 242, 394, 641
DdeI CTNAG 2 cut(s) 384, 390
DpnI GATC 1 cut(s) 60
DpnII GATC 1 cut(s) 58
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco57I CTGAAG 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 629
EcoRII CCWGG 2 cut(s) 174, 244
FaiI YATR 5 cut(s) 123, 332, 498, 561, 649
Fnu4HI GCNGC 1 cut(s) 615
FokI GGATG 2 cut(s) 332, 368
Fsp4HI GCNGC 1 cut(s) 615
FspBI CTAG 1 cut(s) 585
GluI GCNGC 1 cut(s) 615
Hin1I GRCGYC 1 cut(s) 5
HinfI GANTC 4 cut(s) 324, 349, 403, 599
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 1 cut(s) 264
Hpy188I TCNGA 4 cut(s) 348, 414, 445, 581
Hpy188III TCNNGA 2 cut(s) 275, 353
Hpy8I GTNNAC 1 cut(s) 264
Hpy99I CGWCG 1 cut(s) 403
HpyAV CCTTC 3 cut(s) 244, 345, 482
HpyCH4III ACNGT 2 cut(s) 241, 521
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 5 cut(s) 45, 167, 359, 506, 647
HpyF10VI GCNNNNNNNGC 1 cut(s) 117
HpyF3I CTNAG 2 cut(s) 384, 390
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 5
Kzo9I GATC 1 cut(s) 58
LmnI GCTCC 1 cut(s) 611
Lsp1109I GCAGC 1 cut(s) 601
LweI GCATC 2 cut(s) 120, 346
MaeI CTAG 1 cut(s) 585
MaeII ACGT 1 cut(s) 81
MalI GATC 1 cut(s) 60
MboI GATC 1 cut(s) 58
MboII GAAGA 6 cut(s) 5, 11, 92, 95, 458, 594
MhlI GDGCHC 1 cut(s) 482
MluCI AATT 5 cut(s) 197, 418, 429, 435, 629
MlyI GAGTC 1 cut(s) 333
MmeI TCCRAC 2 cut(s) 36, 604
MnlI CCTC 4 cut(s) 82, 85, 544, 583
MroXI GAANNNNTTC 1 cut(s) 439
MseI TTAA 2 cut(s) 140, 408
MslI CAYNNNNRTG 1 cut(s) 300
MspR9I CCNGG 2 cut(s) 176, 246
MvaI CCWGG 2 cut(s) 176, 246
MwoI GCNNNNNNNGC 1 cut(s) 117
NdeII GATC 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 232
PdmI GAANNNNTTC 1 cut(s) 439
PfeI GAWTC 3 cut(s) 349, 403, 599
PkrI GCNGC 1 cut(s) 616
PleI GAGTC 1 cut(s) 332
PpsI GAGTC 1 cut(s) 332
Psp6I CCWGG 2 cut(s) 174, 244
PspGI CCWGG 2 cut(s) 174, 244
PspN4I GGNNCC 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 251
RsaI GTAC 4 cut(s) 217, 243, 395, 642
RsaNI GTAC 4 cut(s) 216, 242, 394, 641
RseI CAYNNNNRTG 1 cut(s) 300
SaqAI TTAA 2 cut(s) 140, 408
SatI GCNGC 1 cut(s) 615
Sau3AI GATC 1 cut(s) 58
ScaI AGTACT 1 cut(s) 395
SchI GAGTC 1 cut(s) 333
ScrFI CCNGG 2 cut(s) 176, 246
SduI GDGCHC 1 cut(s) 482
SetI ASST 9 cut(s) 84, 122, 193, 232, 282, 497, 555, 586, 616
SfaNI GCATC 2 cut(s) 120, 346
SfcI CTRYAG 1 cut(s) 121
SmiMI CAYNNNNRTG 1 cut(s) 300
Sse9I AATT 5 cut(s) 197, 418, 429, 435, 629
SspMI CTAG 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 174, 244
TaaI ACNGT 2 cut(s) 241, 521
TaiI ACGT 1 cut(s) 84
TaqI TCGA 2 cut(s) 401, 633
TasI AATT 5 cut(s) 197, 418, 429, 435, 629
TatI WGTACW 2 cut(s) 215, 393
TfiI GAWTC 3 cut(s) 349, 403, 599
Tru1I TTAA 2 cut(s) 140, 408
Tru9I TTAA 2 cut(s) 140, 408
TscAI CASTG 6 cut(s) 256, 520, 526, 532, 538, 601
TseI GCWGC 1 cut(s) 614
TspGWI ACGGA 1 cut(s) 610
TspRI CASTG 6 cut(s) 256, 520, 526, 532, 538, 601
XapI RAATTY 2 cut(s) 418, 629
XmnI GAANNNNTTC 1 cut(s) 439
XspI CTAG 1 cut(s) 585
ZrmI AGTACT 1 cut(s) 395
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.