FvH4_4g14890

GPI-anchored protein At3g06035-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0008438
Physical Location & Seq
Forward (+)
18509361 .. 18511882
2522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g14890.t1

Sequence Viewer

Length: 627 bp
ATGGCGTCTTCTTCGCTTCGTCTTCTCTTTCCATTGCTCCTGTCTTGCATTGTTCTCTTGAATCACCAAGTCAAATGCGATGAAGCTGATGAGGAGGATAATCTGCTTCAAGGCATCAACAGTTATAGGGCTACTGCCTTGAACTTAACAGCCCTTACAAAGAATGACAATGCAGAGTGCCTAGCAGATGAGATAGCTGACCAATTCAAAAAGCAACCCTGTACAAACACCACTGGTTCTTTCACAGTACCAGGCACTGAAACCCAGTTTGCCAACTTTCCAGACCTTTTAGCCAAATGTCATTTGAATGTCTCAAACACAAGGGATGGAGTCATAATGCCTGCTTGCGTTCCGAATCTGGATCCTACTCTTGTCCTTAACAACTTCACAAAGTCTCAGTACTCATCGAGTCTTAATCAGACAAATTTTACAGGAATCGGAATTGGTTCTGAAGATAACTGGATTGTTGTTATTTTGACCACGAGCACACCTGAAGGAAGCTATGTACCTGCACAAAGCATTGACAGTGCCGGAGCCGATACTGGTACCAGTTCAGTTTCCAAGTCCAAGATCAGTTTGATTCAATACTTGCTGTGTTTGCTGATGGGGTTTGTGTTCCATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0031224 GO:0031225 GO:0031226 GO:0044425 GO:0044459 GO:0044464 GO:0046658 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

209

Amino Acids

22.44

Weight (kDa)

4.59

Isoelectric Point (pI)

34.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At5g19230 PF25884 32 - 159 2e-59 Uncharacterized GPI-anchored protein At5g19230-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 517
Acc65I GGTACC 1 cut(s) 545
AccB1I GGYRCC 1 cut(s) 545
AclWI GGATC 2 cut(s) 356, 369
AcsI RAATTY 1 cut(s) 424
AcuI CTGAAG 2 cut(s) 471, 513
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 5 cut(s) 223, 249, 401, 507, 547
AgsI TTSAA 6 cut(s) 61, 110, 142, 208, 307, 584
AjnI CCWGG 1 cut(s) 250
AluBI AGCT 3 cut(s) 86, 197, 501
AluI AGCT 3 cut(s) 86, 197, 501
Alw21I GWGCWC 1 cut(s) 488
Alw26I GTCTC 2 cut(s) 316, 399
AlwI GGATC 2 cut(s) 356, 369
AlwNI CAGNNNCTG 1 cut(s) 257
ApoI RAATTY 1 cut(s) 424
Asp700I GAANNNNTTC 1 cut(s) 445
Asp718I GGTACC 1 cut(s) 545
AsuHPI GGTGA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 1 cut(s) 545
BauI CACGAG 1 cut(s) 481
BbsI GAAGAC 1 cut(s) 14
Bbv12I GWGCWC 1 cut(s) 488
BccI CCATC 2 cut(s) 320, 598
BciT130I CCWGG 1 cut(s) 252
BcoDI GTCTC 2 cut(s) 316, 399
BfaI CTAG 1 cut(s) 182
BfuAI ACCTGC 1 cut(s) 517
BmcAI AGTACT 1 cut(s) 401
Bme1390I CCNGG 1 cut(s) 252
BmiI GGNNCC 3 cut(s) 363, 535, 547
BmrFI CCNGG 1 cut(s) 252
BmrI ACTGGG 1 cut(s) 259
BmsI GCATC 1 cut(s) 123
BmuI ACTGGG 1 cut(s) 259
BpiI GAAGAC 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 5
Bse1I ACTGG 5 cut(s) 238, 265, 464, 547, 549
Bse3DI GCAATG 1 cut(s) 32
BseBI CCWGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 331
BseMI GCAATG 1 cut(s) 32
BseMII CTCAG 1 cut(s) 410
BseNI ACTGG 5 cut(s) 238, 265, 464, 547, 549
BseRI GAGGAG 1 cut(s) 107
BsgI GTGCAG 1 cut(s) 495
BshNI GGYRCC 1 cut(s) 545
BsiHKAI GWGCWC 1 cut(s) 488
BsiSI CCGG 1 cut(s) 531
BsmAI GTCTC 2 cut(s) 316, 399
Bsp1286I GDGCHC 1 cut(s) 488
Bsp1407I TGTACA 1 cut(s) 221
Bsp143I GATC 2 cut(s) 361, 570
BspCNI CTCAG 1 cut(s) 409
BspLI GGNNCC 3 cut(s) 363, 535, 547
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 2 cut(s) 356, 369
BspT107I GGYRCC 1 cut(s) 545
BsrDI GCAATG 1 cut(s) 32
BsrGI TGTACA 1 cut(s) 221
BsrI ACTGG 5 cut(s) 238, 265, 464, 547, 549
BssMI GATC 2 cut(s) 361, 570
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 481
Bst2BI CACGAG 1 cut(s) 481
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 3 cut(s) 122, 247, 527
BstACI GRCGYC 1 cut(s) 5
BstAUI TGTACA 1 cut(s) 221
BstC8I GCNNGC 2 cut(s) 342, 346
BstDEI CTNAG 1 cut(s) 396
BstF5I GGATG 1 cut(s) 331
BstKTI GATC 2 cut(s) 364, 573
BstMAI GTCTC 2 cut(s) 316, 399
BstMBI GATC 2 cut(s) 361, 570
BstMWI GCNNNNNNNGC 1 cut(s) 598
BstNI CCWGG 1 cut(s) 252
BstSCI CCNGG 1 cut(s) 250
BstV2I GAAGAC 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BtgZI GCGATG 1 cut(s) 93
BtsCI GGATG 1 cut(s) 331
BtsIMutI CAGTG 3 cut(s) 231, 255, 532
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 2 cut(s) 342, 346
CaiI CAGNNNCTG 1 cut(s) 257
Csp6I GTAC 5 cut(s) 222, 248, 400, 506, 546
CviJI RGCY 7 cut(s) 86, 131, 152, 197, 293, 501, 536
CviKI_1 RGCY 7 cut(s) 86, 131, 152, 197, 293, 501, 536
CviQI GTAC 5 cut(s) 222, 248, 400, 506, 546
DdeI CTNAG 1 cut(s) 396
DpnI GATC 2 cut(s) 363, 572
DpnII GATC 2 cut(s) 361, 570
Eco57I CTGAAG 2 cut(s) 471, 513
EcoRII CCWGG 1 cut(s) 250
FaiI YATR 3 cut(s) 126, 335, 504
FokI GGATG 1 cut(s) 338
FspBI CTAG 1 cut(s) 182
HapII CCGG 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 5
HinfI GANTC 6 cut(s) 61, 330, 355, 409, 435, 580
HpaII CCGG 1 cut(s) 531
HphI GGTGA 1 cut(s) 56
Hpy188I TCNGA 4 cut(s) 354, 420, 440, 451
Hpy188III TCNNGA 3 cut(s) 58, 281, 359
HpyAV CCTTC 1 cut(s) 488
HpyCH4III ACNGT 3 cut(s) 122, 247, 527
HpyCH4V TGCA 3 cut(s) 48, 173, 512
HpyF10VI GCNNNNNNNGC 1 cut(s) 598
HpyF3I CTNAG 1 cut(s) 396
Hsp92I GRCGYC 1 cut(s) 5
KpnI GGTACC 1 cut(s) 549
Kzo9I GATC 2 cut(s) 361, 570
LmnI GCTCC 2 cut(s) 42, 533
LweI GCATC 1 cut(s) 123
MaeI CTAG 1 cut(s) 182
MalI GATC 2 cut(s) 363, 572
MboI GATC 2 cut(s) 361, 570
MboII GAAGA 3 cut(s) 3, 14, 464
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 3 cut(s) 203, 424, 441
MlyI GAGTC 2 cut(s) 339, 418
MnlI CCTC 2 cut(s) 85, 88
MroXI GAANNNNTTC 1 cut(s) 445
MseI TTAA 3 cut(s) 146, 378, 414
MslI CAYNNNNRTG 1 cut(s) 306
MspI CCGG 1 cut(s) 531
MspR9I CCNGG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 252
MwoI GCNNNNNNNGC 1 cut(s) 598
NdeII GATC 2 cut(s) 361, 570
NlaIV GGNNCC 3 cut(s) 363, 535, 547
PdmI GAANNNNTTC 1 cut(s) 445
PfeI GAWTC 4 cut(s) 61, 355, 435, 580
PleI GAGTC 2 cut(s) 338, 417
PpsI GAGTC 2 cut(s) 338, 417
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspN4I GGNNCC 3 cut(s) 363, 535, 547
PstNI CAGNNNCTG 1 cut(s) 257
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 5 cut(s) 223, 249, 401, 507, 547
RsaNI GTAC 5 cut(s) 222, 248, 400, 506, 546
RseI CAYNNNNRTG 1 cut(s) 306
SaqAI TTAA 3 cut(s) 146, 378, 414
Sau3AI GATC 2 cut(s) 361, 570
ScaI AGTACT 1 cut(s) 401
SchI GAGTC 2 cut(s) 339, 418
ScrFI CCNGG 1 cut(s) 252
SduI GDGCHC 1 cut(s) 488
SetI ASST 6 cut(s) 88, 199, 288, 493, 503, 511
SfaNI GCATC 1 cut(s) 123
SmiMI CAYNNNNRTG 1 cut(s) 306
Sse9I AATT 3 cut(s) 203, 424, 441
SspMI CTAG 1 cut(s) 182
StyD4I CCNGG 1 cut(s) 250
TaaI ACNGT 3 cut(s) 122, 247, 527
TaqI TCGA 1 cut(s) 407
TasI AATT 3 cut(s) 203, 424, 441
TatI WGTACW 2 cut(s) 221, 399
TfiI GAWTC 4 cut(s) 61, 355, 435, 580
Tru1I TTAA 3 cut(s) 146, 378, 414
Tru9I TTAA 3 cut(s) 146, 378, 414
TscAI CASTG 3 cut(s) 238, 262, 532
TspDTI ATGAA 1 cut(s) 96
TspRI CASTG 3 cut(s) 238, 262, 532
XapI RAATTY 1 cut(s) 424
XmnI GAANNNNTTC 1 cut(s) 445
XspI CTAG 1 cut(s) 182
ZrmI AGTACT 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.