AT3G10390

flowering locus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0005668
Physical Location & Seq
Forward (+)
3229060 .. 3232531
3472 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G10390.3

Sequence Viewer

Length: 2655 bp
ATGGTCTCATTCTCCGCACCAAAGAAACGAAGGAGAGGACGTTCTCAGCGCTCAATGTCTTCGCTTAATTCCCTTCCCGTCCCTAACGTAGGTCTTCTTCCTGGTAACAGTAATTTCGTGTCATCTTCAGCTTCTTCTTCCGGTAGATTTAACGTAGAGGTCGTCAATGGATCAAACCAGACTGTGAAAAGTTATCCTGGGATTGGTGATGAGATCATTACGATTAACAAGGAAGCTACCACTGAAGCTTTGCTTGCTCTCACTGCTGGGTTTCCTGCTGATTCGCTTACCGAGGAAGAGATTGAATTCGGTGTGGTGCCTATTGTTGGAGGCATTGAACAAGTCAATTACATTTTGATTAGGAACCACATTATATCCAAATGGCGAGAAAATATATCTAGTTGGGTCACAAAGGAGATGTTTCTTAATTCGATTCCTAAACATTGTAGTAGTCTCTTAGATTCGGCTTATAATTACTTGGTTACACATGGATATATCAATTTTGGAATAGCACAGGCGATTAAAGATAAGTTTCCTGCTCAGTCAAGCAAGTCTAGTGTGATTATAGTTGGTGCTGGTTTATCTGGGTTGGCTGCAGCTAGGCAACTGATGAGGTTTGGCTTCAAAGTCACGGTTTTGGAGGGTAGGAAACGACCCGGTGGACGGGTTTATACCAAAAAGATGGAAGCTAATAGGGTAGGTGCTGCAGCGGATTTAGGTGGGAGTGTTTTGACTGGAACATTAGGAAATCCTTTAGGGATTATAGCGAGACAGCTTGGTTCTTCTCTCTATAAGGTACGAGACAAGTGTCCTCTTTATAGGGTGGATGGTAAGCCGGTTGATCCAGATGTGGATATAAAGGTTGAGGTAGCGTTTAACCAGCTTCTAGACAAGGCAAGTAAACTTAGGCAGTTGATGGGTGATGTTTCCATGGATGTCTCCCTTGGGGCAGCACTCGAGACATTCAGGCAGGTTTCTGGGAATGATGTGGCCACCGAGGAGATGGGTTTGTTTAACTGGCATCTTGCTAACTTGGAGTATGCAAATGCGGGCCTAGTATCAAAGCTTTCTCTTGCATTCTGGGATCAAGATGACCCATATGATATGGGTGGAGATCACTGCTTTCTGCCTGGAGGAAATGGAAGGCTGGTTCAGGCTTTGGCTGAAAATGTACCAATATTATATGAGAAGACCGTCCAAACAATCAGATATGGTTCAAATGGTGTCAAGGTAACTGCTGGAAATCAAGTGTATGAGGGTGATATGGTGCTGTGTACAGTTCCCCTTGGTGTCCTTAAGAACGGATCCATCAAATTTGTTCCCGAATTACCTCAGAGAAAGCTTGATTGTATAAAGAGATTAGGTTTTGGGTTACTGAATAAAGTTGCAATGCTCTTTCCGTATGTATTTTGGAGCACAGATCTCGATACCTTTGGGCATCTTACTGAGGATCCGAACTATCGAGGTGAATTCTTTCTGTTCTATAGCTACGCACCGGTTGCTGGTGGTGCTCTCTTGATCGCGTTGGTTGCGGGAGAAGCTGCTCATAAGTTTGAGACAATGCCACCCACTGACGCAGTGACTCGTGTTCTTCATATTCTTAGGGGTATTTATGAACCTCAAGGAATAAATGTCCCTGATCCACTTCAAACGGTCTGCACCAGATGGGGTGGGGATCCATTCAGTTTAGGATCTTACTCTAATGTTGCAGTGGGAGCTTCAGGCGATGATTACGATATATTAGCAGAAAGTGTGGGAGATGGAAGGCTTTTCTTTGCCGGAGAAGCCACAACAAGGCGATACCCTGCAACCATGCATGGAGCTTTTGTTACTGGTCTGCGAGAAGCTGCAAACATGGCTCAATCTGCAAAGGCCCGAGGCATAAGGAAAAGAATCGATAGGAACCCATCAAGGAATGCTCATTCCTGTGCCATTCTTCTTGCAGATTTATTTAGGGACCCTGATCTGGAATTTGGGAGTTTTTGCATAATCTTTAGTAGGAGGAATCCAGACCCAAAATCTCCTGCTATTTTGAGGGTAACTCTAAGCGAGCCTCGTAAGAGAAACGAGGACCCAAAAGCAGATCAGCACTCAAACAAGATACTGTTTCAGCAGCTTCAGTCTCATTTCAACCAGCAGCAACAGATTCAGGTGTATACATTATTAACTAGACAACAGGCTCTTGACCTGAGAGAAGTCAGAGGTGGTGATGAAAAGAGACTATACTATCTCTGTGAGACACTTGGAGTGAAACTGGTGGGGAGAAAAGGTCTTGGTGTTGGAGCTGATTCAGTCATTGCTTCTATTAAAGCCGAGCGAACTGGTCGCAAGCTACCATCATCATCTACCTCAGGCACAAAATCTGGTATATTAAAAGCAAAATCCGGTGCATTGAAGCGGAAGATGATCAGAAGGATTAAAGGACCGTTGCCGCTGAAGCAAAGTAATAACAATGGCGTCTCAGAGAGTATAAAATCAGAGTCTTTAGGTAATGGCAAATCCTTAGAGCAACAACAACAACCTAGAATAGTAGAGTCGATAGGAGGAGGTAGCAGCATGCAAGGGGAAAGCAAGTCTTTGAGCACAGGAGTTGCATCTAGTTCTGGTGGTTCCTTACATGTTGGTGGTGAGACGATGAAAAAGATTGAACAATGA

Protein Analysis

884

Amino Acids

95.94

Weight (kDa)

9.31

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIRM PF04433 146 - 220 7.7e-15 SWIRM domain
DAO PF01266 239 - 273 5.7e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 241 - 298 1.6e-11 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 246 - 671 1e-85 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 471
Acc36I ACCTGC 1 cut(s) 961
AccB1I GGYRCC 1 cut(s) 316
AccI GTMKAC 1 cut(s) 2156
AccII CGCG 1 cut(s) 1523
AciI CCGC 6 cut(s) 15, 710, 1049, 1532, 2398, 2432
AcoI YGGCCR 1 cut(s) 990
AcsI RAATTY 4 cut(s) 305, 1313, 1469, 1970
AcuI CTGAAG 5 cut(s) 111, 264, 1704, 2102, 2456
AcyI GRCGYC 1 cut(s) 2457
AfaI GTAC 3 cut(s) 798, 1173, 1276
AfeI AGCGCT 1 cut(s) 50
AfiI CCNNNNNNNGG 7 cut(s) 89, 203, 326, 663, 1502, 1794, 1966
AflII CTTAAG 1 cut(s) 1295
AflIII ACRYGT 1 cut(s) 2617
AgeI ACCGGT 1 cut(s) 1495
AgsI TTSAA 8 cut(s) 305, 338, 625, 1218, 1649, 2131, 2395, 2648
AjnI CCWGG 3 cut(s) 100, 196, 1129
AloI GAACNNNNNNTCC 4 cut(s) 25, 57, 1134, 1166
Alw21I GWGCWC 3 cut(s) 1418, 1513, 2585
Ama87I CYCGRG 2 cut(s) 956, 1875
Aor51HI AGCGCT 1 cut(s) 50
AoxI GGCC 3 cut(s) 990, 1051, 1872
ApoI RAATTY 4 cut(s) 305, 1313, 1469, 1970
AsiGI ACCGGT 1 cut(s) 1495
Asp700I GAANNNNTTC 1 cut(s) 1473
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 5 cut(s) 1051, 1873, 1957, 2071, 2423
AsuC2I CCSGG 1 cut(s) 657
AsuHPI GGTGA 6 cut(s) 218, 932, 1271, 1478, 2219, 2639
AvaI CYCGRG 2 cut(s) 956, 1875
AvaII GGWCC 3 cut(s) 1957, 2071, 2423
AxyI CCTNAGG 1 cut(s) 2350
BalI TGGCCA 1 cut(s) 992
BamHI GGATCC 3 cut(s) 1304, 1450, 1675
BanI GGYRCC 1 cut(s) 316
BarI GAAGNNNNNNTAC 2 cut(s) 81, 113
BauI CACGAG 1 cut(s) 1584
BbsI GAAGAC 3 cut(s) 51, 86, 1196
Bbv12I GWGCWC 3 cut(s) 1418, 1513, 2585
BccI CCATC 9 cut(s) 676, 821, 910, 997, 1316, 1659, 1754, 1915, 2344
BcgI CGANNNNNNTGC 2 cut(s) 1405, 1439
BciT130I CCWGG 3 cut(s) 102, 198, 1131
BclI TGATCA 1 cut(s) 2406
BcnI CCSGG 1 cut(s) 657
BfaI CTAG 8 cut(s) 399, 555, 600, 887, 1055, 2169, 2523, 2598
BfmI CTRYAG 3 cut(s) 594, 705, 1483
BfoI RGCGCY 1 cut(s) 52
BfrI CTTAAG 1 cut(s) 1295
BfuAI ACCTGC 1 cut(s) 961
BglII AGATCT 1 cut(s) 1420
Bme1390I CCNGG 4 cut(s) 102, 198, 657, 1131
Bme18I GGWCC 3 cut(s) 1957, 2071, 2423
BmeT110I CYCGRG 2 cut(s) 956, 1875
BmgT120I GGNCC 5 cut(s) 1051, 1873, 1957, 2071, 2423
BmrFI CCNGG 4 cut(s) 102, 198, 657, 1131
BmsI GCATC 3 cut(s) 1030, 1447, 2603
BoxI GACNNNNGTC 1 cut(s) 807
BpiI GAAGAC 3 cut(s) 51, 86, 1196
BplI GAGNNNNNCTC 2 cut(s) 2026, 2058
BpmI CTGGAG 1 cut(s) 1152
BpuEI CTTGAG 1 cut(s) 1605
BpuMI CCSGG 1 cut(s) 657
Bsa29I ATCGAT 1 cut(s) 1896
BsaHI GRCGYC 1 cut(s) 2457
BsaI GGTCTC 1 cut(s) 10
BsaJI CCNNGG 7 cut(s) 197, 291, 930, 943, 996, 1285, 1876
BsaWI WCCGGW 3 cut(s) 140, 1495, 2384
BsaXI ACNNNNNCTCC 6 cut(s) 25, 55, 1812, 1842, 2254, 2284
Bsc4I CCNNNNNNNGG 7 cut(s) 89, 203, 326, 663, 1502, 1794, 1966
Bse118I RCCGGY 2 cut(s) 835, 1495
Bse1I ACTGG 5 cut(s) 739, 1022, 1837, 2259, 2326
Bse21I CCTNAGG 1 cut(s) 2350
Bse3DI GCAATG 2 cut(s) 1395, 2295
BseBI CCWGG 3 cut(s) 102, 198, 1131
BseCI ATCGAT 1 cut(s) 1896
BseDI CCNNGG 7 cut(s) 197, 291, 930, 943, 996, 1285, 1876
BseGI GGATG 2 cut(s) 832, 940
BseLI CCNNNNNNNGG 7 cut(s) 89, 203, 326, 663, 1502, 1794, 1966
BseMI GCAATG 2 cut(s) 1395, 2295
BseMII CTCAG 7 cut(s) 59, 554, 1346, 1437, 2180, 2364, 2475
BseNI ACTGG 5 cut(s) 739, 1022, 1837, 2259, 2326
BseRI GAGGAG 2 cut(s) 1013, 2559
BseYI CCCAGC 1 cut(s) 266
BsgI GTGCAG 1 cut(s) 1642
Bsh1236I CGCG 1 cut(s) 1523
BshFI GGCC 3 cut(s) 992, 1053, 1874
BshNI GGYRCC 1 cut(s) 316
BshTI ACCGGT 1 cut(s) 1495
BshVI ATCGAT 1 cut(s) 1896
BsiHKAI GWGCWC 3 cut(s) 1418, 1513, 2585
BsiHKCI CYCGRG 2 cut(s) 956, 1875
BsiSI CCGG 6 cut(s) 141, 657, 836, 1496, 1779, 2385
BslFI GGGAC 3 cut(s) 65, 1619, 1970
BslI CCNNNNNNNGG 7 cut(s) 89, 203, 326, 663, 1502, 1794, 1966
BsmBI CGTCTC 2 cut(s) 2464, 2624
BsmFI GGGAC 3 cut(s) 65, 1619, 1970
BsmI GAATGC 2 cut(s) 1076, 1921
BsnI GGCC 3 cut(s) 992, 1053, 1874
Bso31I GGTCTC 1 cut(s) 10
BsoBI CYCGRG 2 cut(s) 956, 1875
Bsp1286I GDGCHC 3 cut(s) 1418, 1513, 2585
Bsp1407I TGTACA 1 cut(s) 1274
Bsp19I CCATGG 1 cut(s) 930
BspACI CCGC 6 cut(s) 15, 710, 1049, 1532, 2398, 2432
BspANI GGCC 3 cut(s) 992, 1053, 1874
BspCNI CTCAG 7 cut(s) 58, 553, 1345, 1438, 2181, 2363, 2474
BspDI ATCGAT 1 cut(s) 1896
BspFNI CGCG 1 cut(s) 1523
BspMAI CTGCAG 2 cut(s) 598, 709
BspMI ACCTGC 1 cut(s) 961
BspT107I GGYRCC 1 cut(s) 316
BspTI CTTAAG 1 cut(s) 1295
BspTNI GGTCTC 1 cut(s) 10
BsrDI GCAATG 2 cut(s) 1395, 2295
BsrFI RCCGGY 2 cut(s) 835, 1495
BsrGI TGTACA 1 cut(s) 1274
BsrI ACTGG 5 cut(s) 739, 1022, 1837, 2259, 2326
BssAI RCCGGY 2 cut(s) 835, 1495
BssECI CCNNGG 7 cut(s) 197, 291, 930, 943, 996, 1285, 1876
BssNAI GTATAC 1 cut(s) 2157
BssNI GRCGYC 1 cut(s) 2457
BssSI CACGAG 1 cut(s) 1584
BssT1I CCWWGG 3 cut(s) 930, 943, 1285
Bst1107I GTATAC 1 cut(s) 2157
Bst2BI CACGAG 1 cut(s) 1584
Bst2UI CCWGG 3 cut(s) 102, 198, 1131
Bst4CI ACNGT 8 cut(s) 110, 184, 634, 1195, 1279, 1654, 2106, 2427
Bst6I CTCTTC 1 cut(s) 291
BstACI GRCGYC 1 cut(s) 2457
BstAFI CTTAAG 1 cut(s) 1295
BstAPI GCANNNNNTGC 1 cut(s) 1499
BstAUI TGTACA 1 cut(s) 1274
BstC8I GCNNGC 5 cut(s) 255, 1051, 2051, 2330, 2558
BstDSI CCRYGG 1 cut(s) 930
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 2 cut(s) 832, 940
BstFNI CGCG 1 cut(s) 1523
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 1 cut(s) 51
BstNI CCWGG 3 cut(s) 102, 198, 1131
BstNSI RCATGY 2 cut(s) 2560, 2621
BstPAI GACNNNNGTC 1 cut(s) 807
BstSCI CCNGG 4 cut(s) 100, 196, 655, 1129
BstSFI CTRYAG 3 cut(s) 594, 705, 1483
BstUI CGCG 1 cut(s) 1523
BstV2I GAAGAC 3 cut(s) 51, 86, 1196
BstX2I RGATCY 5 cut(s) 1304, 1420, 1450, 1675, 1691
BstXI CCANNNNNNTGG 1 cut(s) 682
BstYI RGATCY 5 cut(s) 1304, 1420, 1450, 1675, 1691
BstZ17I GTATAC 1 cut(s) 2157
Bsu15I ATCGAT 1 cut(s) 1896
Bsu36I CCTNAGG 1 cut(s) 2350
BsuRI GGCC 3 cut(s) 992, 1053, 1874
BsuTUI ATCGAT 1 cut(s) 1896
BtgI CCRYGG 1 cut(s) 930
BtgZI GCGATG 1 cut(s) 1740
BtsCI GGATG 2 cut(s) 832, 940
BtsI GCAGTG 4 cut(s) 261, 1117, 1584, 1716
BtsIMutI CAGTG 6 cut(s) 240, 261, 1117, 1569, 1584, 1716
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 5 cut(s) 255, 1051, 2051, 2330, 2558
CfoI GCGC 1 cut(s) 51
Cfr10I RCCGGY 2 cut(s) 835, 1495
Cfr13I GGNCC 5 cut(s) 1051, 1873, 1957, 2071, 2423
ClaI ATCGAT 1 cut(s) 1896
CseI GACGC 2 cut(s) 1583, 2446
Csp6I GTAC 3 cut(s) 797, 1172, 1275
CspAI ACCGGT 1 cut(s) 1495
CviAII CATG 7 cut(s) 488, 931, 1813, 1817, 1855, 2557, 2618
CviQI GTAC 3 cut(s) 797, 1172, 1275
EaeI YGGCCR 1 cut(s) 990
Eam1104I CTCTTC 1 cut(s) 291
EarI CTCTTC 1 cut(s) 291
Eco130I CCWWGG 3 cut(s) 930, 943, 1285
Eco31I GGTCTC 1 cut(s) 10
Eco47I GGWCC 3 cut(s) 1957, 2071, 2423
Eco47III AGCGCT 1 cut(s) 50
Eco57I CTGAAG 5 cut(s) 111, 264, 1704, 2102, 2456
Eco81I CCTNAGG 1 cut(s) 2350
Eco88I CYCGRG 2 cut(s) 956, 1875
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoO109I RGGNCCY 2 cut(s) 1957, 2071
EcoRI GAATTC 2 cut(s) 305, 1469
EcoRII CCWGG 3 cut(s) 100, 196, 1129
EcoT14I CCWWGG 3 cut(s) 930, 943, 1285
EcoT22I ATGCAT 1 cut(s) 1818
ErhI CCWWGG 3 cut(s) 930, 943, 1285
Esp3I CGTCTC 2 cut(s) 2464, 2624
FaeI CATG 7 cut(s) 491, 934, 1816, 1820, 1858, 2560, 2621
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 2560, 2592
FaqI GGGAC 3 cut(s) 65, 1619, 1970
FatI CATG 7 cut(s) 487, 930, 1812, 1816, 1854, 2556, 2617
FauI CCCGC 2 cut(s) 1042, 1525
FauNDI CATATG 1 cut(s) 1099
FbaI TGATCA 1 cut(s) 2406
FblI GTMKAC 1 cut(s) 2156
FokI GGATG 2 cut(s) 839, 947
FspBI CTAG 8 cut(s) 399, 555, 600, 887, 1055, 2169, 2523, 2598
GlaI GCGC 1 cut(s) 50
GsaI CCCAGC 1 cut(s) 270
GsuI CTGGAG 1 cut(s) 1152
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 3 cut(s) 992, 1053, 1874
HapII CCGG 6 cut(s) 141, 657, 836, 1496, 1779, 2385
HgaI GACGC 2 cut(s) 1583, 2446
HhaI GCGC 1 cut(s) 51
Hin1I GRCGYC 1 cut(s) 2457
Hin1II CATG 7 cut(s) 491, 934, 1816, 1820, 1858, 2560, 2621
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HindIII AAGCTT 3 cut(s) 246, 1064, 1340
HpaII CCGG 6 cut(s) 141, 657, 836, 1496, 1779, 2385
HphI GGTGA 6 cut(s) 218, 932, 1271, 1478, 2219, 2639
Hpy166II GTNNAC 4 cut(s) 662, 902, 1275, 2157
Hpy188I TCNGA 7 cut(s) 1208, 1335, 1455, 2201, 2411, 2464, 2479
Hpy8I GTNNAC 4 cut(s) 662, 902, 1275, 2157
HpyAV CCTTC 5 cut(s) 24, 83, 1137, 1758, 2406
HpyCH4III ACNGT 8 cut(s) 110, 184, 634, 1195, 1279, 1654, 2106, 2427
HpyCH4IV ACGT 3 cut(s) 40, 87, 153
HpySE526I ACGT 3 cut(s) 40, 87, 153
Hsp92I GRCGYC 1 cut(s) 2457
Hsp92II CATG 7 cut(s) 491, 934, 1816, 1820, 1858, 2560, 2621
HspAI GCGC 1 cut(s) 49
KflI GGGWCCC 1 cut(s) 1957
Ksp22I TGATCA 1 cut(s) 2406
LmnI GCTCC 4 cut(s) 1413, 1715, 1820, 2282
LweI GCATC 3 cut(s) 1030, 1447, 2603
MaeI CTAG 8 cut(s) 399, 555, 600, 887, 1055, 2169, 2523, 2598
MaeII ACGT 3 cut(s) 40, 87, 153
MaeIII GTNAC 9 cut(s) 104, 406, 481, 628, 1231, 1371, 1579, 1828, 2038
MflI RGATCY 5 cut(s) 1304, 1420, 1450, 1675, 1691
MhlI GDGCHC 3 cut(s) 1418, 1513, 2585
MlsI TGGCCA 1 cut(s) 992
MluNI TGGCCA 1 cut(s) 992
MlyI GAGTC 3 cut(s) 1576, 2489, 2543
MmeI TCCRAC 2 cut(s) 307, 2260
Mox20I TGGCCA 1 cut(s) 992
Mph1103I ATGCAT 1 cut(s) 1818
MroXI GAANNNNTTC 1 cut(s) 1473
MscI TGGCCA 1 cut(s) 992
MslI CAYNNNNRTG 2 cut(s) 1926, 2622
Msp20I TGGCCA 1 cut(s) 992
MspA1I CMGCKG 2 cut(s) 710, 2434
MspCI CTTAAG 1 cut(s) 1295
MspI CCGG 6 cut(s) 141, 657, 836, 1496, 1779, 2385
MspR9I CCNGG 4 cut(s) 102, 198, 657, 1131
Mva1269I GAATGC 2 cut(s) 1076, 1921
MvaI CCWGG 3 cut(s) 102, 198, 1131
MvnI CGCG 1 cut(s) 1523
NciI CCSGG 1 cut(s) 657
NcoI CCATGG 1 cut(s) 930
NdeI CATATG 1 cut(s) 1099
NlaIII CATG 7 cut(s) 491, 934, 1816, 1820, 1858, 2560, 2621
NmeAIII GCCGAG 1 cut(s) 2338
NmuCI GTSAC 3 cut(s) 406, 628, 1579
NsiI ATGCAT 1 cut(s) 1818
NspI RCATGY 2 cut(s) 2560, 2621
PaeI GCATGC 1 cut(s) 2560
PaeR7I CTCGAG 1 cut(s) 956
PciI ACATGT 1 cut(s) 2617
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PctI GAATGC 2 cut(s) 1076, 1921
PdmI GAANNNNTTC 1 cut(s) 1473
PfeI GAWTC 7 cut(s) 281, 433, 461, 1893, 2005, 2146, 2288
PinAI ACCGGT 1 cut(s) 1495
PleI GAGTC 3 cut(s) 1576, 2488, 2542
PpsI GAGTC 3 cut(s) 1576, 2488, 2542
PpuMI RGGWCCY 2 cut(s) 1957, 2071
PscI ACATGT 1 cut(s) 2617
PshAI GACNNNNGTC 1 cut(s) 807
PsiI TTATAA 1 cut(s) 471
Psp5II RGGWCCY 2 cut(s) 1957, 2071
Psp6I CCWGG 3 cut(s) 100, 196, 1129
PspFI CCCAGC 1 cut(s) 266
PspGI CCWGG 3 cut(s) 100, 196, 1129
PspPI GGNCC 5 cut(s) 1051, 1873, 1957, 2071, 2423
PspPPI RGGWCCY 2 cut(s) 1957, 2071
PstI CTGCAG 2 cut(s) 598, 709
PsuI RGATCY 5 cut(s) 1304, 1420, 1450, 1675, 1691
RsaI GTAC 3 cut(s) 798, 1173, 1276
RsaNI GTAC 3 cut(s) 797, 1172, 1275
RseI CAYNNNNRTG 2 cut(s) 1926, 2622
Sau96I GGNCC 5 cut(s) 1051, 1873, 1957, 2071, 2423
SchI GAGTC 3 cut(s) 1576, 2489, 2543
ScrFI CCNGG 4 cut(s) 102, 198, 657, 1131
SduI GDGCHC 3 cut(s) 1418, 1513, 2585
SfaNI GCATC 3 cut(s) 1030, 1447, 2603
SfcI CTRYAG 3 cut(s) 594, 705, 1483
Sfr274I CTCGAG 1 cut(s) 956
SinI GGWCC 3 cut(s) 1957, 2071, 2423
SlaI CTCGAG 1 cut(s) 956
SmiMI CAYNNNNRTG 2 cut(s) 1926, 2622
SmlI CTYRAG 3 cut(s) 956, 1295, 1620
SmoI CTYRAG 3 cut(s) 956, 1295, 1620
SphI GCATGC 1 cut(s) 2560
SsiI CCGC 6 cut(s) 15, 710, 1049, 1532, 2398, 2432
SspI AATATT 1 cut(s) 1179
SspMI CTAG 8 cut(s) 399, 555, 600, 887, 1055, 2169, 2523, 2598
StyD4I CCNGG 4 cut(s) 100, 196, 655, 1129
StyI CCWWGG 3 cut(s) 930, 943, 1285
TaaI ACNGT 8 cut(s) 110, 184, 634, 1195, 1279, 1654, 2106, 2427
TaiI ACGT 3 cut(s) 43, 90, 156
TaqI TCGA 6 cut(s) 431, 957, 1425, 1462, 1896, 2537
TatI WGTACW 1 cut(s) 1274
TauI GCSGC 1 cut(s) 2434
TfiI GAWTC 7 cut(s) 281, 433, 461, 1893, 2005, 2146, 2288
TscAI CASTG 6 cut(s) 247, 268, 1124, 1576, 1584, 1716
TseFI GTSAC 3 cut(s) 406, 628, 1579
Tsp45I GTSAC 3 cut(s) 406, 628, 1579
TspDTI ATGAA 4 cut(s) 1583, 1629, 2226, 2651
TspGWI ACGGA 2 cut(s) 1317, 1389
TspRI CASTG 6 cut(s) 247, 268, 1124, 1576, 1584, 1716
Vha464I CTTAAG 1 cut(s) 1295
VpaK11BI GGWCC 3 cut(s) 1957, 2071, 2423
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 4 cut(s) 305, 1313, 1469, 1970
XbaI TCTAGA 1 cut(s) 886
XceI RCATGY 2 cut(s) 2560, 2621
XcmI CCANNNNNNNNNTGG 1 cut(s) 1000
XhoI CTCGAG 1 cut(s) 956
XmiI GTMKAC 1 cut(s) 2156
XmnI GAANNNNTTC 1 cut(s) 1473
XspI CTAG 8 cut(s) 399, 555, 600, 887, 1055, 2169, 2523, 2598
Zsp2I ATGCAT 1 cut(s) 1818
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.