Rw1G027350

Lysine-specific histone demethylase 1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005668
Physical Location & Seq
Reverse (-)
54838466 .. 54844415
5950 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G027350.1

Sequence Viewer

Length: 2373 bp
ATGTCCGACGAGATCATTGTGATCAATAAGGAGTCCACGGCCGAGGCGTTGATCGCGCTCTCGGCTGGGTTTCCAGCCGATTCTCTCACAGAGGAGGAAATCGATTTCGGGGTTATTCGGGTTATTGGGGGAATAGAGCAGGTCAATTATATTCTCATTCGGAACCATATCATTGCAAAATGGCGCGAAAATGTGTCCAATTGGGTCACAAAGGATATTTTTGCTAACTCTATACCCAAGCATTGTCATGCTCTTTTAGACTCTACTTATAACTATCTGGTTTCACATGGATATATTAATTTTGGGATTGCACCGGCCATTAAAGAGAAGATTCCCGCGGAGCCTAGTAAGCCGAGTGTGATTATCATTGGTGCTGGGCTTGCTGGTCTTGCTGCTGCTAGGCAAATGATGCGTTTTGGGTTTAAAGTGACTGTTTTGGAGGGTAGGAAGCGTGCAGGTGGGCGGGTGTACACTAAGAAAATGGAGGGAGGAATTAGGGCATGTCAAGCAGCGGCGGATTTAGGGGGTAGTGTTTTGACTGGTACATTAGGGAACCCACTTGGCATTGTTGCTCGCCAATTGGGCTACTCGCTTCATAAGATTAGAGACAAGTGCCCACTTTATAATGTGGAGGGGAAACCGGTAGATCATGATATGGATATGAAGGTGGAAAGTGTTTTTAATCATCTTTTAGATAAGGCAAGTAGGCTTAGGCAGTTAATGGGTGGGGTTTCGGTTGATGTGTCTCTTGGTTCGGCATTGGAAACATTCTGGCATGTTTCTGGGAATGCTGTGAATGCTGAGGAGATGAACTTGTTCAATTGGCATCTTGCGAATTTGGAATACGCAAATGCAGGTTTACTGTCGAAACTTTCGCTTGCATTTTGGGACCAAGATGATCCATATGATATGGGAGGGGACCATTGCTTCTTACCTGGTGGTAATGGAAGGCTGGTGCAGGCTTTGGCAGAGAATGTGCCGATCCTATATGAGAAAACTGTTCACACCATAAGGTATGGTAGTGACGGGGTACAGATTCTTGCTGGAAGCCAGGCTTTTGAGGGTGATATGGTTTTGTGCACTGTTCCGCTTGGTGTTCTGAAGAGTGGGTCCATCAAGTTTATTCCTGAGTTGCCTCAGCGAAAGCTTGATGGAATTAAGAGGTTGGGTTTTGGTTTGTTGAATAAGGTTGCAATGCTTTTTCCTCATGTGTTTTGGGGCACAGATCTTGATACATTCGGCCATCTGTCTGATGATTCAAGCCGTCGAGGGGAATTCTTCCTGTTTTACAGCTATGCAACAGTTGCTGGTGGTCCTCTCTTGCTTGCTTTAGTAGCAGGTGAAGCTGCGCATAAGTTTGAGAGCATGCCCCCTACGGATGCTGTGACCCGGGTTCTTCAGATTCTTAAGGGTATTTATGAACCACAAGGAATTAGTGTCCCAGAGCCCATCCAAACAGTCTGTACCAGATGGGGAAGTGACCCCTTCAGCCTCGGTTCTTACTCTAATGTTGCAGTTGGGGCATCAGGAGATGATTATGATATCTTAGCAGAAAGTGTTGGAGATGGGCGACTTTTCTTTGCCGGTGAGGCTACCAATAGGAGATATCCTGCAACTATGCACGGAGCTTTCCTTAGTGGGCTCAGAGAAGCTGCAAATATGGCCAACTATGCTAATGCTCGAGCTCTGAGGATAAAGCTTAACAGGAACCCATCAAAGAATGCCCATTCTTGTGCTTCCCTTCTTGCAGATTTATTCAGAGAGCCTGATTTAGAATTTGGGAGTTTTTCTGTTATTTTTGGTCGAAGAAATGCTGATCCCAAGTCAACAGCAATTTTAAGGGTGACATTTAATGACCCTCGGAAGAAGAGTCATGACGGTTCAAGACCTGATCAACAGCACTCAAATAAGTTACTTTTTCAGCAGCTTCAGTCACATTTTAATCAGCAGCAGCAGCTTCATGTTTACACTTTGTTATCTAGGCAGCAGGCTCTTGAGCTCCGAGAAGTACGAGGTGGTGATGAGATGAGGTTGAATTACCTCTGTGAAAAGCTTGGAGTGAAGCTTGTGGGGAGAAAGGGTTTGGGGCCTTCAGCTGATTCTGTAATTGCCTTAATAAAAGCTGAGAGGGGCAATCGTAAACCTGCTTCAACTTTATCATCTTTTAAGTCTGGTACATCGAAGCTGAAACCTGGCACTTTGAAACGAAAATTGGTCAGAAGGGCTAAAATATTGCGCAGCATTAATGCGTCTCCCCTTTCTGCTAATTCAAACTTGGCTAGTAAACTCCCAGAGGAAAATAAGAACAGTGTTCAAGTAGTCTCCAACACACTCGGTTCAGGCCAAAACGATTCTGAGGTGTTGAAGCAATGA

Protein Analysis

790

Amino Acids

86.26

Weight (kDa)

9.0

Isoelectric Point (pI)

37.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SWIRM PF04433 27 - 101 3.3e-14 SWIRM domain
Pyr_redox_2 PF07992 119 - 164 5.8e-06 Pyridine nucleotide-disulphide oxidoreductase
NAD_binding_8 PF13450 122 - 181 4.4e-10 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 127 - 554 1.1e-83 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 270, 624
AarI CACCTGC 2 cut(s) 446, 1328
Acc16I TGCGCA 2 cut(s) 1350, 2237
Acc36I ACCTGC 5 cut(s) 130, 446, 845, 1328, 2152
AccII CGCG 3 cut(s) 56, 186, 338
AciI CCGC 6 cut(s) 336, 338, 463, 512, 515, 1088
AclWI GGATC 3 cut(s) 893, 976, 1811
AcoI YGGCCR 4 cut(s) 39, 315, 1240, 1662
AcsI RAATTY 3 cut(s) 835, 1274, 1775
AcuI CTGAAG 5 cut(s) 1121, 1382, 1471, 1913, 2076
AfaI GTAC 6 cut(s) 470, 544, 1032, 1465, 2010, 2176
AfiI CCNNNNNNNGG 1 cut(s) 1270
AflII CTTAAG 1 cut(s) 1406
AgeI ACCGGT 1 cut(s) 640
AjnI CCWGG 3 cut(s) 934, 1050, 2191
AjuI GAANNNNNNNTTGG 2 cut(s) 2195, 2227
Alw21I GWGCWC 3 cut(s) 1082, 1687, 2001
Alw26I GTCTC 4 cut(s) 600, 750, 2256, 2326
Alw44I GTGCAC 1 cut(s) 1078
AlwI GGATC 3 cut(s) 893, 976, 1811
AlwNI CAGNNNCTG 1 cut(s) 1307
Ama87I CYCGRG 2 cut(s) 1389, 1680
AoxI GGCC 6 cut(s) 39, 315, 1240, 1662, 2087, 2341
ApaLI GTGCAC 1 cut(s) 1078
ApoI RAATTY 3 cut(s) 835, 1274, 1775
AseI ATTAAT 2 cut(s) 297, 2244
AsiGI ACCGGT 1 cut(s) 640
Asp700I GAANNNNTTC 1 cut(s) 815
AspLEI GCGC 4 cut(s) 58, 186, 1351, 2238
AspS9I GGNCC 5 cut(s) 889, 919, 1110, 1313, 2087
AsuC2I CCSGG 2 cut(s) 1390, 1391
AsuHPI GGTGA 5 cut(s) 1076, 1352, 1598, 1855, 2030
AvaI CYCGRG 2 cut(s) 1389, 1680
AvaII GGWCC 4 cut(s) 889, 919, 1110, 1313
BaeGI GKGCMC 3 cut(s) 617, 1082, 1223
BalI TGGCCA 1 cut(s) 1664
BanII GRGCYC 4 cut(s) 1449, 1644, 1687, 2001
BarI GAAGNNNNNNTAC 2 cut(s) 2131, 2163
Bbv12I GWGCWC 3 cut(s) 1082, 1687, 2001
BbvCI CCTCAGC 2 cut(s) 801, 1137
BccI CCATC 7 cut(s) 1121, 1145, 1251, 1457, 1464, 1559, 1720
BceAI ACGGC 2 cut(s) 54, 1248
BciT130I CCWGG 3 cut(s) 936, 1052, 2193
BclI TGATCA 2 cut(s) 21, 1891
BcnI CCSGG 2 cut(s) 1390, 1391
BcoDI GTCTC 4 cut(s) 600, 750, 2256, 2326
BfaI CTAG 4 cut(s) 345, 399, 1980, 2280
BfrI CTTAAG 1 cut(s) 1406
BfuAI ACCTGC 5 cut(s) 130, 446, 845, 1328, 2152
BglI GCCNNNNNGGC 2 cut(s) 582, 1589
BglII AGATCT 1 cut(s) 1225
Bme1390I CCNGG 5 cut(s) 936, 1052, 1390, 1391, 2193
Bme18I GGWCC 4 cut(s) 889, 919, 1110, 1313
BmeT110I CYCGRG 2 cut(s) 1389, 1680
BmgT120I GGNCC 5 cut(s) 889, 919, 1110, 1313, 2087
BmiI GGNNCC 8 cut(s) 164, 342, 554, 890, 920, 1111, 1709, 2088
BmrFI CCNGG 5 cut(s) 936, 1052, 1390, 1391, 2193
BmsI GCATC 4 cut(s) 399, 835, 1369, 1532
Bpu10I CCTNAGC 3 cut(s) 710, 801, 1137
BpuEI CTTGAG 1 cut(s) 2015
BpuMI CCSGG 2 cut(s) 1390, 1391
Bsa29I ATCGAT 1 cut(s) 102
BsaJI CCNNGG 6 cut(s) 36, 42, 336, 1389, 1492, 1859
BsaWI WCCGGW 1 cut(s) 640
BsaXI ACNNNNNCTCC 2 cut(s) 2065, 2095
Bsc4I CCNNNNNNNGG 1 cut(s) 1270
Bse118I RCCGGY 3 cut(s) 313, 640, 1583
Bse1I ACTGG 1 cut(s) 544
Bse3DI GCAATG 3 cut(s) 171, 922, 1200
BseBI CCWGG 3 cut(s) 936, 1052, 2193
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 6 cut(s) 36, 42, 336, 1389, 1492, 1859
BseGI GGATG 2 cut(s) 1384, 1449
BseLI CCNNNNNNNGG 1 cut(s) 1270
BseMI GCAATG 3 cut(s) 171, 922, 1200
BseMII CTCAG 7 cut(s) 792, 1119, 1151, 1657, 1679, 2115, 2346
BseNI ACTGG 1 cut(s) 544
BseRI GAGGAG 2 cut(s) 107, 818
BseSI GKGCMC 3 cut(s) 617, 1082, 1223
BseX3I CGGCCG 1 cut(s) 39
BseYI CCCAGC 2 cut(s) 65, 374
BsgI GTGCAG 2 cut(s) 474, 977
Bsh1236I CGCG 3 cut(s) 56, 186, 338
Bsh1285I CGRYCG 1 cut(s) 42
BshFI GGCC 6 cut(s) 41, 317, 1242, 1664, 2089, 2343
BshTI ACCGGT 1 cut(s) 640
BshVI ATCGAT 1 cut(s) 102
BsiEI CGRYCG 1 cut(s) 42
BsiHKAI GWGCWC 3 cut(s) 1082, 1687, 2001
BsiHKCI CYCGRG 2 cut(s) 1389, 1680
BsiSI CCGG 4 cut(s) 314, 641, 1390, 1584
BslFI GGGAC 3 cut(s) 902, 932, 1424
BslI CCNNNNNNNGG 1 cut(s) 1270
BsmAI GTCTC 4 cut(s) 600, 750, 2256, 2326
BsmBI CGTCTC 1 cut(s) 2256
BsmFI GGGAC 3 cut(s) 902, 932, 1424
BsmI GAATGC 3 cut(s) 793, 802, 1726
BsnI GGCC 6 cut(s) 41, 317, 1242, 1664, 2089, 2343
BsoBI CYCGRG 2 cut(s) 1389, 1680
Bsp1286I GDGCHC 7 cut(s) 617, 1082, 1223, 1449, 1644, 1687, 2001
Bsp1407I TGTACA 1 cut(s) 468
Bsp143I GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
BspACI CCGC 6 cut(s) 336, 338, 463, 512, 515, 1088
BspANI GGCC 6 cut(s) 41, 317, 1242, 1664, 2089, 2343
BspCNI CTCAG 7 cut(s) 793, 1120, 1150, 1656, 1680, 2116, 2347
BspDI ATCGAT 1 cut(s) 102
BspFNI CGCG 3 cut(s) 56, 186, 338
BspHI TCATGA 2 cut(s) 649, 1873
BspLI GGNNCC 8 cut(s) 164, 342, 554, 890, 920, 1111, 1709, 2088
BspMI ACCTGC 5 cut(s) 130, 446, 845, 1328, 2152
BspPI GGATC 3 cut(s) 893, 976, 1811
BspTI CTTAAG 1 cut(s) 1406
BsrDI GCAATG 3 cut(s) 171, 922, 1200
BsrFI RCCGGY 3 cut(s) 313, 640, 1583
BsrGI TGTACA 1 cut(s) 468
BsrI ACTGG 1 cut(s) 544
BssAI RCCGGY 3 cut(s) 313, 640, 1583
BssECI CCNNGG 6 cut(s) 36, 42, 336, 1389, 1492, 1859
BssMI GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
Bst2UI CCWGG 3 cut(s) 936, 1052, 2193
Bst4CI ACNGT 8 cut(s) 433, 864, 1000, 1084, 1303, 1459, 1880, 2309
Bst6I CTCTTC 2 cut(s) 1097, 1862
BstAFI CTTAAG 1 cut(s) 1406
BstAPI GCANNNNNTGC 2 cut(s) 409, 1304
BstAUI TGTACA 1 cut(s) 468
BstC8I GCNNGC 8 cut(s) 381, 453, 574, 879, 960, 1326, 1367, 1989
BstDSI CCRYGG 2 cut(s) 36, 336
BstF5I GGATG 2 cut(s) 1384, 1449
BstFNI CGCG 3 cut(s) 56, 186, 338
BstHHI GCGC 4 cut(s) 58, 186, 1351, 2238
BstKTI GATC 9 cut(s) 15, 24, 54, 649, 901, 984, 1228, 1819, 1894
BstMAI GTCTC 4 cut(s) 600, 750, 2256, 2326
BstMBI GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
BstMCI CGRYCG 1 cut(s) 42
BstNI CCWGG 3 cut(s) 936, 1052, 2193
BstNSI RCATGY 3 cut(s) 504, 779, 1369
BstSCI CCNGG 5 cut(s) 934, 1050, 1388, 1389, 2191
BstSLI GKGCMC 3 cut(s) 617, 1082, 1223
BstUI CGCG 3 cut(s) 56, 186, 338
BstX2I RGATCY 1 cut(s) 1225
BstYI RGATCY 1 cut(s) 1225
BstZI CGGCCG 1 cut(s) 39
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 6 cut(s) 41, 317, 1242, 1664, 2089, 2343
BsuTUI ATCGAT 1 cut(s) 102
BtgI CCRYGG 2 cut(s) 36, 336
BtsCI GGATG 2 cut(s) 1384, 1449
BtsIMutI CAGTG 2 cut(s) 1080, 2314
BveI ACCTGC 5 cut(s) 130, 446, 845, 1328, 2152
Cac8I GCNNGC 8 cut(s) 381, 453, 574, 879, 960, 1326, 1367, 1989
CaiI CAGNNNCTG 1 cut(s) 1307
CciI TCATGA 2 cut(s) 649, 1873
CfoI GCGC 4 cut(s) 58, 186, 1351, 2238
Cfr10I RCCGGY 3 cut(s) 313, 640, 1583
Cfr13I GGNCC 5 cut(s) 889, 919, 1110, 1313, 2087
Cfr42I CCGCGG 1 cut(s) 339
Cfr9I CCCGGG 1 cut(s) 1389
ClaI ATCGAT 1 cut(s) 102
CseI GACGC 1 cut(s) 2238
CsiI ACCWGGT 1 cut(s) 934
Csp6I GTAC 6 cut(s) 469, 543, 1031, 1464, 2009, 2175
CspAI ACCGGT 1 cut(s) 640
CviAII CATG 9 cut(s) 248, 287, 501, 650, 776, 1208, 1366, 1874, 1961
CviQI GTAC 6 cut(s) 469, 543, 1031, 1464, 2009, 2175
DpnI GATC 9 cut(s) 14, 23, 53, 648, 900, 983, 1227, 1818, 1893
DpnII GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
DraI TTTAAA 1 cut(s) 424
EaeI YGGCCR 4 cut(s) 39, 315, 1240, 1662
EagI CGGCCG 1 cut(s) 39
Eam1104I CTCTTC 2 cut(s) 1097, 1862
EarI CTCTTC 2 cut(s) 1097, 1862
EciI GGCGGA 1 cut(s) 530
Ecl136II GAGCTC 2 cut(s) 1685, 1999
EclXI CGGCCG 1 cut(s) 39
Eco24I GRGCYC 4 cut(s) 1449, 1644, 1687, 2001
Eco32I GATATC 2 cut(s) 1543, 1607
Eco47I GGWCC 4 cut(s) 889, 919, 1110, 1313
Eco52I CGGCCG 1 cut(s) 39
Eco53kI GAGCTC 2 cut(s) 1685, 1999
Eco57I CTGAAG 5 cut(s) 1121, 1382, 1471, 1913, 2076
Eco88I CYCGRG 2 cut(s) 1389, 1680
EcoICRI GAGCTC 2 cut(s) 1685, 1999
EcoO109I RGGNCCY 1 cut(s) 2087
EcoRI GAATTC 1 cut(s) 1274
EcoRII CCWGG 3 cut(s) 934, 1050, 2191
EcoRV GATATC 2 cut(s) 1543, 1607
EcoT38I GRGCYC 4 cut(s) 1449, 1644, 1687, 2001
Esp3I CGTCTC 1 cut(s) 2256
FaeI CATG 9 cut(s) 251, 290, 504, 653, 779, 1211, 1369, 1877, 1964
FalI AAGNNNNNCTT 2 cut(s) 1039, 1071
FaqI GGGAC 3 cut(s) 902, 932, 1424
FatI CATG 9 cut(s) 247, 286, 500, 649, 775, 1207, 1365, 1873, 1960
FauI CCCGC 2 cut(s) 343, 456
FauNDI CATATG 1 cut(s) 904
FbaI TGATCA 2 cut(s) 21, 1891
FokI GGATG 2 cut(s) 1391, 1436
FriOI GRGCYC 4 cut(s) 1449, 1644, 1687, 2001
FspBI CTAG 4 cut(s) 345, 399, 1980, 2280
FspI TGCGCA 2 cut(s) 1350, 2237
GlaI GCGC 4 cut(s) 57, 185, 1350, 2237
GsaI CCCAGC 2 cut(s) 69, 378
HaeIII GGCC 6 cut(s) 41, 317, 1242, 1664, 2089, 2343
HapII CCGG 4 cut(s) 314, 641, 1390, 1584
HgaI GACGC 1 cut(s) 2238
HhaI GCGC 4 cut(s) 58, 186, 1351, 2238
Hin1II CATG 9 cut(s) 251, 290, 504, 653, 779, 1211, 1369, 1877, 1964
Hin6I GCGC 4 cut(s) 56, 184, 1349, 2236
HinP1I GCGC 4 cut(s) 56, 184, 1349, 2236
HincII GTYRAC 1 cut(s) 1827
HindII GTYRAC 1 cut(s) 1827
HindIII AAGCTT 4 cut(s) 1145, 1697, 2051, 2063
HpaII CCGG 4 cut(s) 314, 641, 1390, 1584
HphI GGTGA 5 cut(s) 1076, 1352, 1598, 1855, 2030
Hpy188III TCNNGA 7 cut(s) 650, 1127, 1229, 1527, 1874, 1884, 1994
Hpy99I CGWCG 2 cut(s) 11, 1269
HpyAV CCTTC 6 cut(s) 658, 942, 1495, 1751, 2100, 2214
HpyCH4III ACNGT 8 cut(s) 433, 864, 1000, 1084, 1303, 1459, 1880, 2309
Hsp92II CATG 9 cut(s) 251, 290, 504, 653, 779, 1211, 1369, 1877, 1964
HspAI GCGC 4 cut(s) 56, 184, 1349, 2236
Ksp22I TGATCA 2 cut(s) 21, 1891
KspI CCGCGG 1 cut(s) 339
Kzo9I GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
LmnI GCTCC 3 cut(s) 340, 1625, 2004
LweI GCATC 4 cut(s) 399, 835, 1369, 1532
MabI ACCWGGT 1 cut(s) 934
MaeI CTAG 4 cut(s) 345, 399, 1980, 2280
MaeIII GTNAC 8 cut(s) 205, 427, 1022, 1384, 1478, 1843, 1911, 1932
MalI GATC 9 cut(s) 14, 23, 53, 648, 900, 983, 1227, 1818, 1893
MboI GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
MboII GAAGA 7 cut(s) 340, 1114, 1270, 1388, 1818, 1876, 1879
MfeI CAATTG 3 cut(s) 199, 578, 820
MflI RGATCY 1 cut(s) 1225
MhlI GDGCHC 7 cut(s) 617, 1082, 1223, 1449, 1644, 1687, 2001
MlsI TGGCCA 1 cut(s) 1664
MluNI TGGCCA 1 cut(s) 1664
MlyI GAGTC 3 cut(s) 41, 254, 1879
MmeI TCCRAC 3 cut(s) 30, 1540, 2349
Mox20I TGGCCA 1 cut(s) 1664
MroXI GAANNNNTTC 1 cut(s) 815
MscI TGGCCA 1 cut(s) 1664
MslI CAYNNNNRTG 2 cut(s) 246, 1731
Msp20I TGGCCA 1 cut(s) 1664
MspA1I CMGCKG 3 cut(s) 338, 512, 2096
MspCI CTTAAG 1 cut(s) 1406
MspI CCGG 4 cut(s) 314, 641, 1390, 1584
MspR9I CCNGG 5 cut(s) 936, 1052, 1390, 1391, 2193
MunI CAATTG 3 cut(s) 199, 578, 820
Mva1269I GAATGC 3 cut(s) 793, 802, 1726
MvaI CCWGG 3 cut(s) 936, 1052, 2193
MvnI CGCG 3 cut(s) 56, 186, 338
NciI CCSGG 2 cut(s) 1390, 1391
NdeI CATATG 1 cut(s) 904
NdeII GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
NlaIII CATG 9 cut(s) 251, 290, 504, 653, 779, 1211, 1369, 1877, 1964
NlaIV GGNNCC 8 cut(s) 164, 342, 554, 890, 920, 1111, 1709, 2088
NmeAIII GCCGAG 3 cut(s) 41, 67, 378
NmuCI GTSAC 7 cut(s) 205, 427, 1022, 1384, 1478, 1843, 1932
NsbI TGCGCA 2 cut(s) 1350, 2237
NspI RCATGY 3 cut(s) 504, 779, 1369
PaeI GCATGC 1 cut(s) 1369
PaeR7I CTCGAG 1 cut(s) 1680
PagI TCATGA 2 cut(s) 649, 1873
PaqCI CACCTGC 2 cut(s) 446, 1328
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PctI GAATGC 3 cut(s) 793, 802, 1726
PdmI GAANNNNTTC 1 cut(s) 815
PfeI GAWTC 7 cut(s) 80, 331, 1036, 1256, 1402, 2099, 2351
PinAI ACCGGT 1 cut(s) 640
PleI GAGTC 3 cut(s) 40, 254, 1878
PpsI GAGTC 3 cut(s) 40, 254, 1878
PshBI ATTAAT 2 cut(s) 297, 2244
PsiI TTATAA 2 cut(s) 270, 624
Psp124BI GAGCTC 2 cut(s) 1687, 2001
Psp6I CCWGG 3 cut(s) 934, 1050, 2191
PspFI CCCAGC 2 cut(s) 65, 374
PspGI CCWGG 3 cut(s) 934, 1050, 2191
PspN4I GGNNCC 8 cut(s) 164, 342, 554, 890, 920, 1111, 1709, 2088
PspPI GGNCC 5 cut(s) 889, 919, 1110, 1313, 2087
PspXI VCTCGAGB 1 cut(s) 1680
PstNI CAGNNNCTG 1 cut(s) 1307
PsuI RGATCY 1 cut(s) 1225
PvuII CAGCTG 1 cut(s) 2096
RsaI GTAC 6 cut(s) 470, 544, 1032, 1465, 2010, 2176
RsaNI GTAC 6 cut(s) 469, 543, 1031, 1464, 2009, 2175
RseI CAYNNNNRTG 2 cut(s) 246, 1731
SacI GAGCTC 2 cut(s) 1687, 2001
SacII CCGCGG 1 cut(s) 339
Sau3AI GATC 9 cut(s) 12, 21, 51, 646, 898, 981, 1225, 1816, 1891
Sau96I GGNCC 5 cut(s) 889, 919, 1110, 1313, 2087
SchI GAGTC 3 cut(s) 41, 254, 1879
ScrFI CCNGG 5 cut(s) 936, 1052, 1390, 1391, 2193
SduI GDGCHC 7 cut(s) 617, 1082, 1223, 1449, 1644, 1687, 2001
SexAI ACCWGGT 1 cut(s) 934
SfaNI GCATC 4 cut(s) 399, 835, 1369, 1532
Sfr274I CTCGAG 1 cut(s) 1680
Sfr303I CCGCGG 1 cut(s) 339
SgrBI CCGCGG 1 cut(s) 339
SinI GGWCC 4 cut(s) 889, 919, 1110, 1313
SlaI CTCGAG 1 cut(s) 1680
SmaI CCCGGG 1 cut(s) 1391
SmiMI CAYNNNNRTG 2 cut(s) 246, 1731
SmlI CTYRAG 3 cut(s) 1406, 1680, 1994
SmoI CTYRAG 3 cut(s) 1406, 1680, 1994
SphI GCATGC 1 cut(s) 1369
SsiI CCGC 6 cut(s) 336, 338, 463, 512, 515, 1088
SspI AATATT 1 cut(s) 2232
SspMI CTAG 4 cut(s) 345, 399, 1980, 2280
SstI GAGCTC 2 cut(s) 1687, 2001
StyD4I CCNGG 5 cut(s) 934, 1050, 1388, 1389, 2191
TaaI ACNGT 8 cut(s) 433, 864, 1000, 1084, 1303, 1459, 1880, 2309
TaqI TCGA 6 cut(s) 102, 866, 1267, 1681, 1804, 2180
TatI WGTACW 1 cut(s) 468
TauI GCSGC 1 cut(s) 515
TfiI GAWTC 7 cut(s) 80, 331, 1036, 1256, 1402, 2099, 2351
TscAI CASTG 2 cut(s) 1087, 2314
TseFI GTSAC 7 cut(s) 205, 427, 1022, 1384, 1478, 1843, 1932
Tsp45I GTSAC 7 cut(s) 205, 427, 1022, 1384, 1478, 1843, 1932
TspDTI ATGAA 5 cut(s) 584, 677, 824, 1434, 1949
TspGWI ACGGA 2 cut(s) 1391, 1638
TspMI CCCGGG 1 cut(s) 1389
TspRI CASTG 2 cut(s) 1087, 2314
Vha464I CTTAAG 1 cut(s) 1406
VneI GTGCAC 1 cut(s) 1078
VpaK11BI GGWCC 4 cut(s) 889, 919, 1110, 1313
VspI ATTAAT 2 cut(s) 297, 2244
XapI RAATTY 3 cut(s) 835, 1274, 1775
XceI RCATGY 3 cut(s) 504, 779, 1369
XhoI CTCGAG 1 cut(s) 1680
XmaI CCCGGG 1 cut(s) 1389
XmnI GAANNNNTTC 1 cut(s) 815
XspI CTAG 4 cut(s) 345, 399, 1980, 2280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.